LOCUS V037631 8104 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V037631 VERSION V037631 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers LTR 234..414 /note="truncated 5' long terminal repeat (LTR) from HIV-1" /label="5' LTR (truncated)" misc_feature 458..583 /note="packaging signal of human immunodeficiency virus type 1" /label="HIV-1 Ψ" misc_feature 1076..1309 /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." /label="RRE" CDS 1493..1537 /codon_start=1 /note="recognized by the 2H10 single-chain llama nanobody" /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /transl_table=1 /translation="KNEQELLELDKWASL" /label="gp41 peptide" misc_feature 1804..1920 /note="central polypurine tract and central termination sequence of HIV-1" /label="cPPT/CTS" promoter 2010..2213 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" CDS 2302..2958 /label="CDO1(NM_001323565)" /note="CDO1(NM_001323565)" /gene="CDO1" CDS 2965..3030 /codon_start=1 /product="three tandem FLAG® epitope tags, followed by an enterokinase cleavage site" /transl_table=1 /translation="DYKDHDGDYKDHDIDYKDDDDK" /label="3xFLAG" promoter 3074..3285 /note="core promoter for human elongation factor EF-1α" /label="EF-1α core promoter" LTR 3298..3566 /note="truncated 5' long terminal repeat (LTR) from human T-cell leukemia virus (HTLV) type 1" /label="5' LTR (truncated)" CDS 3593..4192 /codon_start=1 /gene="pac from Streptomyces alboniger" /note="confers resistance to puromycin" /product="puromycin N-acetyltransferase" /transl_table=1 /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*" /label="PuroR" misc_feature 4199..4787 /note="woodchuck hepatitis virus posttranscriptional regulatory element" /label="WPRE" LTR 4861..5094 /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" /label="3' LTR (ΔU3)" polyA_signal 5166..5287 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" rep_origin 5306..5441 /note="SV40 origin of replication" /label="SV40 ori" primer_bind complement(5479..5495) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 5503..5519 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(5527..5557) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 5572..5593 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(5881..6469) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(6640..7500) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(7501..7605) /gene="bla" /label="AmpR promoter" primer_bind 8079..8095 /note="common sequencing primer, one of multiple similar variants" /label="M13 fwd" ORIGIN 1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt 1921 atcgatacta gtattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca 1981 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc 2041 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga 2101 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat 2161 tgacgcaaat gggcggtagg cgtgtacggt gggaggttta tataagcaga gctcgtttag 2221 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagattct 2281 agagctagcg aattcgccac catggaacag accgaagtgc tgaagccacg gaccctggct 2341 gatctgatcc gcatcctgca ccagctcttt gccggcgatg aggtcaatgt agaggaggtg 2401 caggccatca tggaagccta cgagagcgac cccaccgagt gggcaatgta cgccaagttc 2461 gaccagtaca gtcgtggtcg tggtttgcag tttgttgttg gtggtggcag tggtggtggt 2521 tggttgtggt atacccgaaa tcttgtggat caaggaaatg gaaaatttaa tctgatgatt 2581 ctctgttggg gtgaaggaca tggcagcagt attcatgatc ataccaactc ccactgcttt 2641 ctgaagatgc tacagggaaa tctaaaggag acattatttg cctggcctga caaaaaatcc 2701 aatgagatgg tcaagaagtc tgaaagagtc ttgagggaaa accagtgtgc ctacatcaat 2761 gattccattg gcttacatcg agtagagaac atcagccata cggaacctgc tgtgagcctt 2821 cacttgtaca gtccaccttt tgatacatgc catgcctttg atcaaagaac aggacataaa 2881 aacaaagtca caatgacatt ccatagtaaa tttggaatca gaactccaaa tgcaacttcg 2941 ggctcgctgg agaacaacgg atccgactac aaagaccatg acggtgatta taaagatcat 3001 gacatcgact acaaggatga cgatgacaag tagcggccgc gaaggatctg cgatcgctcc 3061 ggtgcccgtc agtgggcaga gcgcacatcg cccacagtcc ccgagaagtt ggggggaggg 3121 gtcggcaatt gaacgggtgc ctagagaagg tggcgcgggg taaactggga aagtgatgtc 3181 gtgtactggc tccgcctttt tcccgagggt gggggagaac cgtatataag tgcagtagtc 3241 gccgtgaacg ttctttttcg caacgggttt gccgccagaa cacagctgaa gcttcgaggg 3301 gctcgcatct ctccttcacg cgcccgccgc cctacctgag gccgccatcc acgccggttg 3361 agtcgcgttc tgccgcctcc cgcctgtggt gcctcctgaa ctgcgtccgc cgtctaggta 3421 agtttaaagc tcaggtcgag accgggcctt tgtccggcgc tcccttggag cctacctaga 3481 ctcagccggc tctccacgct ttgcctgacc ctgcttgctc aactctacgt ctttgtttcg 3541 ttttctgttc tgcgccgtta cagatccaag ctgtgaccgg cgcctacgct agatgaccga 3601 gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc ccccgggccg tacgcaccct 3661 cgccgccgcg ttcgccgact accccgccac gcgccacacc gtcgacccgg accgccacat 3721 cgagcgggtc accgagctgc aagaactctt cctcacgcgc gtcgggctcg acatcggcaa 3781 ggtgtgggtc gcggacgacg gcgccgcggt ggcggtctgg accacgccgg agagcgtcga 3841 agcgggggcg gtgttcgccg agatcggccc gcgcatggcc gagttgagcg gttcccggct 3901 ggccgcgcag caacagatgg aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg 3961 gttcctggcc accgtcggcg tctcgcccga ccaccagggc aagggtctgg gcagcgccgt 4021 cgtgctcccc ggagtggagg cggccgagcg cgccggggtg cccgccttcc tggagacctc 4081 cgcgccccgc aacctcccct tctacgagcg gctcggcttc accgtcaccg ccgacgtcga 4141 ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag cccggtgcct gagtcgacaa 4201 tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc 4261 ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat 4321 ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg 4381 gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg 4441 ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat 4501 tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt 4561 gggcactgac aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc 4621 ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa 4681 tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg 4741 ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcctg gtacctttaa 4801 gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac 4861 tggaagggct aattcactcc caacgaaaat aagatctgct ttttgcttgt actgggtctc 4921 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 4981 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 5041 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtagta 5101 gttcatgtca tcttattatt cagtatttat aacttgcaaa gaaatgaata tcagagagtg 5161 agaggaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt 5221 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg 5281 tatcttatca tgtctggctc tagctatccc gcccctaact ccgcccagtt ccgcccattc 5341 tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc 5401 tgagctattc cagaagtagt gaggaggctt ttttggaggc ctagactttt gcagagacgg 5461 cccaaattcg taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat 5521 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag 5581 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg 5641 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc 5701 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc 5761 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa 5821 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt 5881 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg 5941 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg 6001 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag 6061 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc 6121 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa 6181 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg 6241 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc 6301 taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac 6361 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg 6421 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt 6481 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt 6541 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa 6601 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga 6661 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt 6721 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg 6781 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga 6841 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga 6901 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg 6961 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc 7021 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc 7081 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca 7141 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac 7201 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg 7261 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc 7321 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg 7381 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac 7441 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat 7501 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata 7561 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa 7621 agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg 7681 tatcacgagg ccctttcgtc tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat 7741 gcagctcccg gagacggtca cagcttgtct gtaagcggat gccgggagca gacaagcccg 7801 tcagggcgcg tcagcgggtg ttggcgggtg tcggggctgg cttaactatg cggcatcaga 7861 gcagattgta ctgagagtgc accatatgcg gtgtgaaata ccgcacagat gcgtaaggag 7921 aaaataccgc atcaggcgcc attcgccatt caggctgcgc aactgttggg aagggcgatc 7981 ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt 8041 aagttgggta acgccagggt tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa 8101 gctg //