LOCUS       V040755                12076 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V040755
VERSION     V040755
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2317..3966
                     /EC_number=""
                     /codon_start=1
                     /gene="luc+"
                     /note="enhanced&nbsp;luc+ version of the luciferase gene"
                     /product="firefly luciferase"
                     /protein_id=""
                     /transl_table=1
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
                     /label="luciferase"
     CDS             3997..6102
                     /label="CEACAM5(NM_004363)"
                     /note="CEACAM5(NM_004363)"
                     /gene="CEACAM5"
     CDS             6109..6174
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        6218..6429
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             6442..6710
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      6739..6748
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             6769..7431
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             7501..7554
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             7555..8154
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    8155..8743
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             8817..9050
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    9122..9243
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      9283..9418
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(9451..9467)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    9475..9491
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(9499..9529)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    9544..9565
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(9853..10441)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(10612..11472)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(11473..11577)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     12051..12067
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc ctgatacgaa ctcggaattc gccaccatgg aagacgccaa aaacataaag
     2341 aaaggcccgg cgccattcta tccgctggaa gatggaaccg ctggagagca actgcataag
     2401 gctatgaaga gatacgccct ggttcctgga acaattgctt ttacagatgc acatatcgag
     2461 gtggacatca cttacgctga gtacttcgaa atgtccgttc ggttggcaga agctatgaaa
     2521 cgatatgggc tgaatacaaa tcacagaatc gtcgtatgca gtgaaaactc tcttcaattc
     2581 tttatgccgg tgttgggcgc gttatttatc ggagttgcag ttgcgcccgc gaacgacatt
     2641 tataatgaac gtgaattgct caacagtatg ggcatttcgc agcctaccgt ggtgttcgtt
     2701 tccaaaaagg ggttgcaaaa aattttgaac gtgcaaaaaa agctcccaat catccaaaaa
     2761 attattatca tggattctaa aacggattac cagggatttc agtcgatgta cacgttcgtc
     2821 acatctcatc tacctcccgg ttttaatgaa tacgattttg tgccagagtc cttcgatagg
     2881 gacaagacaa ttgcactgat catgaactcc tctggatcta ctggtctgcc taaaggtgtc
     2941 gctctgcctc atagaactgc ctgcgtgaga ttctcgcatg ccagagatcc tatttttggc
     3001 aatcaaatca ttccggatac tgcgatttta agtgttgttc cattccatca cggttttgga
     3061 atgtttacta cactcggata tttgatatgt ggatttcgag tcgtcttaat gtatagattt
     3121 gaagaagagc tgtttctgag gagccttcag gattacaaga ttcaaagtgc gctgctggtg
     3181 ccaaccctat tctccttctt cgccaaaagc actctgattg acaaatacga tttatctaat
     3241 ttacacgaaa ttgcttctgg tggcgctccc ctctctaagg aagtcgggga agcggttgcc
     3301 aagaggttcc atctgccagg tatcaggcaa ggatatgggc tcactgagac tacatcagct
     3361 attctgatta cacccgaggg ggatgataaa ccgggcgcgg tcggtaaagt tgttccattt
     3421 tttgaagcga aggttgtgga tctggatacc gggaaaacgc tgggcgttaa tcaaagaggc
     3481 gaactgtgtg tgagaggtcc tatgattatg tccggttatg taaacaatcc ggaagcgacc
     3541 aacgccttga ttgacaagga tggatggcta cattctggag acatagctta ctgggacgaa
     3601 gacgaacact tcttcatcgt tgaccgcctg aagtctctga ttaagtacaa aggctatcag
     3661 gtggctcccg ctgaattgga atccatcttg ctccaacacc ccaacatctt cgacgcaggt
     3721 gtcgcaggtc ttcccgacga tgacgccggt gaacttcccg ccgccgttgt tgttttggag
     3781 cacggaaaga cgatgacgga aaaagagatc gtggattacg tcgccagtca agtaacaacc
     3841 gcgaaaaagt tgcgcggagg agttgtgttt gtggacgaag taccgaaagg tcttaccgga
     3901 aaactcgacg caagaaaaat cagagagatc ctcataaagg ccaagaaggg cggaaagatc
     3961 gccgtgggtg ggggcggttc aggtggcggt ggatccatgg agtctccctc ggcccctccc
     4021 cacagatggt gcatcccctg gcagaggctc ctgctcacag cctcacttct aaccttctgg
     4081 aacccgccca ccactgccaa gctcactatt gaatccacgc cgttcaatgt cgcagagggg
     4141 aaggaggtgc ttctacttgt ccacaatctg ccccagcatc tttttggcta cagctggtac
     4201 aaaggtgaaa gagtggatgg caaccgtcaa attataggat atgtaatagg aactcaacaa
     4261 gctaccccag ggcccgcata cagtggtcga gagataatat accccaatgc atccctgctg
     4321 atccagaaca tcatccagaa tgacacagga ttctacaccc tacacgtcat aaagtcagat
     4381 cttgtgaatg aagaagcaac tggccagttc cgggtatacc cggagctgcc caagccctcc
     4441 atctccagca acaactccaa acccgtggag gacaaggatg ctgtggcctt cacctgtgaa
     4501 cctgagactc aggacgcaac ctacctgtgg tgggtaaaca atcagagcct cccggtcagt
     4561 cccaggctgc agctgtccaa tggcaacagg accctcactc tattcaatgt cacaagaaat
     4621 gacacagcaa gctacaaatg tgaaacccag aacccagtga gtgccaggcg cagtgattca
     4681 gtcatcctga atgtcctcta tggcccggat gcccccacca tttcccctct aaacacatct
     4741 tacagatcag gggaaaatct gaacctctcc tgccacgcag cctctaaccc acctgcacag
     4801 tactcttggt ttgtcaatgg gactttccag caatccaccc aagagctctt tatccccaac
     4861 atcactgtga ataatagtgg atcctatacg tgccaagccc ataactcaga cactggcctc
     4921 aataggacca cagtcacgac gatcacagtc tatgcagagc cacccaaacc cttcatcacc
     4981 agcaacaact ccaaccccgt ggaggatgag gatgctgtag ccttaacctg tgaacctgag
     5041 attcagaaca caacctacct gtggtgggta aataatcaga gcctcccggt cagtcccagg
     5101 ctgcagctgt ccaatgacaa caggaccctc actctactca gtgtcacaag gaatgatgta
     5161 ggaccctatg agtgtggaat ccagaacgaa ttaagtgttg accacagcga cccagtcatc
     5221 ctgaatgtcc tctatggccc agacgacccc accatttccc cctcatacac ctattaccgt
     5281 ccaggggtga acctcagcct ctcctgccat gcagcctcta acccacctgc acagtattct
     5341 tggctgattg atgggaacat ccagcaacac acacaagagc tctttatctc caacatcact
     5401 gagaagaaca gcggactcta tacctgccag gccaataact cagccagtgg ccacagcagg
     5461 actacagtca agacaatcac agtctctgcg gagctgccca agccctccat ctccagcaac
     5521 aactccaaac ccgtggagga caaggatgct gtggccttca cctgtgaacc tgaggctcag
     5581 aacacaacct acctgtggtg ggtaaatggt cagagcctcc cagtcagtcc caggctgcag
     5641 ctgtccaatg gcaacaggac cctcactcta ttcaatgtca caagaaatga cgcaagagcc
     5701 tatgtatgtg gaatccagaa ctcagtgagt gcaaaccgca gtgacccagt caccctggat
     5761 gtcctctatg ggccggacac ccccatcatt tcccccccag actcgtctta cctttcggga
     5821 gcgaacctca acctctcctg ccactcggcc tctaacccat ccccgcagta ttcttggcgt
     5881 atcaatggga taccgcagca acacacacaa gttctcttta tcgccaaaat cacgccaaat
     5941 aataacggga cctatgcctg ttttgtctct aacttggcta ctggccgcaa taattccata
     6001 gtcaagagca tcacagtctc tgcatctgga acttctcctg gtctctcagc tggggccact
     6061 gtcggcatca tgattggagt gctggttggg gttgctctga tactcgagga ctacaaagac
     6121 catgacggtg attataaaga tcatgacatc gactacaagg atgacgatga caagtagcgg
     6181 ccgcgaagga tctgcgatcg ctccggtgcc cgtcagtggg cagagcgcac atcgcccaca
     6241 gtccccgaga agttgggggg aggggtcggc aattgaacgg gtgcctagag aaggtggcgc
     6301 ggggtaaact gggaaagtga tgtcgtgtac tggctccgcc tttttcccga gggtggggga
     6361 gaaccgtata taagtgcagt agtcgccgtg aacgttcttt ttcgcaacgg gtttgccgcc
     6421 agaacacagc tgaagcttcg aggggctcgc atctctcctt cacgcgcccg ccgccctacc
     6481 tgaggccgcc atccacgccg gttgagtcgc gttctgccgc ctcccgcctg tggtgcctcc
     6541 tgaactgcgt ccgccgtcta ggtaagttta aagctcaggt cgagaccggg cctttgtccg
     6601 gcgctccctt ggagcctacc tagactcagc cggctctcca cgctttgcct gaccctgctt
     6661 gctcaactct acgtctttgt ttcgttttct gttctgcgcc gttacagatc caagctgtga
     6721 ccggcgccta cgctagacgc caccatggag agcgacgaga gcggcctgcc cgccatggag
     6781 atcgagtgcc gcatcaccgg caccctgaac ggcgtggagt tcgagctggt gggcggcgga
     6841 gagggcaccc ccaagcaggg ccgcatgacc aacaagatga agagcaccaa aggcgccctg
     6901 accttcagcc cctacctgct gagccacgtg atgggctacg gcttctacca cttcggcacc
     6961 taccccagcg gctacgagaa ccccttcctg cacgccatca acaacggcgg ctacaccaac
     7021 acccgcatcg agaagtacga ggacggcggc gtgctgcacg tgagcttcag ctaccgctac
     7081 gaggccggcc gcgtgatcgg cgacttcaag gtggtgggca ccggcttccc cgaggacagc
     7141 gtgatcttca ccgacaagat catccgcagc aacgccaccg tggagcacct gcaccccatg
     7201 ggcgataacg tgctggtggg cagcttcgcc cgcaccttca gcctgcgcga cggcggctac
     7261 tacagcttcg tggtggacag ccacatgcac ttcaagagcg ccatccaccc cagcatcctg
     7321 cagaacgggg gccccatgtt cgccttccgc cgcgtggagg agctgcacag caacaccgag
     7381 ctgggcatcg tggagtacca gcacgccttc aagaccccca tcgccttcgc cagatcccgc
     7441 gctcagtcgt ccaattctgc cgtggacggc accgccggac ccggctccac cggatctcgc
     7501 gagggcagag gaagtcttct aacatgcggt gacgtggagg agaatcccgg ccctatgacc
     7561 gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg tccccagggc cgtacgcacc
     7621 ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca ccgtcgatcc ggaccgccac
     7681 atcgagcggg tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc
     7741 aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc
     7801 gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg
     7861 ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg
     7921 tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc
     7981 gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt cctggagacc
     8041 tccgcgcccc gcaacctccc cttctacgag cggctcggct tcaccgtcac cgccgacgtc
     8101 gaggtgcccg aaggaccgcg cacctggtgc atgacccgca agcccggtgc ctgaaatcaa
     8161 cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt
     8221 acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct
     8281 ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc
     8341 gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg
     8401 ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc
     8461 acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc
     8521 actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct gctcgcctgt
     8581 gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca
     8641 gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt
     8701 cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcctggtac ctttaagacc
     8761 aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg ggggactgga
     8821 agggctaatt cactcccaac gaagataaga tctgcttttt gcttgtactg ggtctctctg
     8881 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
     8941 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
     9001 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtagtagttc
     9061 atgtcatctt attattcagt atttataact tgcaaagaaa tgaatatcag agagtgagag
     9121 gaacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac
     9181 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc
     9241 ttatcatgtc tggctctagc tatcccgccc ctaactccgc ccatcccgcc cctaactccg
     9301 cccagttccg cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc
     9361 gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta
     9421 gacttttgca gagaccaaat tcgtaatcat gtcatagctg tttcctgtgt gaaattgtta
     9481 tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc
     9541 ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg
     9601 aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg
     9661 tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
     9721 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
     9781 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
     9841 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
     9901 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     9961 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
    10021 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
    10081 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
    10141 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
    10201 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
    10261 gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct
    10321 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
    10381 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
    10441 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
    10501 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
    10561 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
    10621 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
    10681 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
    10741 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc
    10801 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
    10861 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
    10921 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
    10981 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
    11041 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
    11101 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
    11161 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
    11221 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
    11281 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
    11341 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
    11401 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
    11461 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
    11521 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
    11581 atttccccga aaagtgccac ctgacgtcta agaaaccatt attatcatga cattaaccta
    11641 taaaaatagg cgtatcacga ggccctttcg tctcgcgcgt ttcggtgatg acggtgaaaa
    11701 cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag
    11761 cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggct ggcttaacta
    11821 tgcggcatca gagcagattg tactgagagt gcaccatatg cggtgtgaaa taccgcacag
    11881 atgcgtaagg agaaaatacc gcatcaggcg ccattcgcca ttcaggctgc gcaactgttg
    11941 ggaagggcga tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc
    12001 tgcaaggcga ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac
    12061 ggccagtgcc aagctg
//
