LOCUS       V029503                11737 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V029503
VERSION     V029503
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2317..5763
                     /label="CFAP251(NM_144668)"
                     /note="CFAP251(NM_144668)"
                     /gene="CFAP251"
     CDS             5770..5835
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        5879..6090
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             6103..6371
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      6400..6409
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             6430..7092
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             7162..7215
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             7216..7815
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    7816..8404
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             8478..8711
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    8783..8904
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      8944..9079
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(9112..9128)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    9136..9152
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(9160..9190)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    9205..9226
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(9514..10102)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(10273..11133)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(11134..11238)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     11712..11728
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc ctgatacgaa ctcggaattc gccaccatgt cagatgcagc agaagctccc
     2341 cgagaagcaa caggagaaaa tggagaaaca gaaatgaaag aagaggagga acctaatcca
     2401 aattataaag aagtagaaga tccacaacag gaatcaaaag atgacacaat agcatggaga
     2461 gagtctcagg aggaggagag gaaaacgggc gaggaggaag gggaggagga ggggaaggag
     2521 gacaaaaaga ttgtcatgga agaaactgag gaaaaggctg gagaagtcca agagaaggag
     2581 gcttcaggaa tacaggaaga aaccacagta gagccccaag aagtcacagc gtccatgatc
     2641 cgtttggaga cacagattac tgattcccag tcaatcacat caggaatttt cccaaaaacc
     2701 caaagaggta gcaagtcaaa gctttcctta caattggagg atgcagaaac agatgagctt
     2761 ttaagagacc tgagcacaca aattgaattt cttgatttgg atcaaatcag tcctgaggaa
     2821 caacagatta gttcccctga aaggcagccc tcaggagagc ttgaggagaa aaccgaccgg
     2881 atgccccaag atgaactggg acaagaaaga agggacttgg agccagaaaa cagagaggag
     2941 ggacaagaaa ggagagtatc cgacatccag tccaaagcag ggatctcccg ggagtcactg
     3001 gtgtccagca ccacagagga cattctgttt caaaaggata aaagcacccc ggtgtatccc
     3061 ttgaccatga cctggtcgtt tggatggaac agttctcttc ctgtttacta tattcgagag
     3121 gaaaggcaga gagttcttct gtatgtttgt gctcacactg cgatcatcta caacgtgttc
     3181 aggaacaatc aataccacct tcagggccac gccaatatta tctcctgcct ctgcgtcagt
     3241 gaagacaggc ggtggatcgc cacagcagac aaagggccag actgcctggt gattatatgg
     3301 gactccttca caggtattcc tgtgcacaca atatttgaca gctgccctga agggaatggc
     3361 atcatggcca tggccatgac ccacgacgcc aagtatctgg caaccatctc agatgctgaa
     3421 gtccagaagg tatgcatctg gaagtggact ttggcagtgg aaacgccagc atgcactctc
     3481 gaactcccca cagagtacgg tgttcagaac tacgttactt ttaacccaac aaataataaa
     3541 gaattggtga gcaatagtaa aacacgggca atatattatg catggtatga agagagggat
     3601 acactggctc acagtgcccc acttttaact gaaaaaacct tcaacaagct tgtgggaaag
     3661 tttagccagt ccatctttca cttgaattta acacaaatac tctcagccac aatggaaggg
     3721 aagctggttg tctgggacat acaccgccca ccctcatctg cctccacctt tttgggcttt
     3781 ccctatatca agccttgtaa attggttcat ttgcagaaag agggtatcac ggtacttacc
     3841 acaattgata gctacattgt cacaggtgac attaagggga acattaagtt ctatgatcac
     3901 accctgtcta ttgttaactg gtacagtcac ttgaaactgg gcgccataag aactctgtcc
     3961 ttttcaaaga ccccagcaac tcctcctact gaaaaatcaa actatcctcc tgactgcact
     4021 ttaaaaggtg acctttttgt cttaaggaat tttatcattg gaacatctga tgccgcggtg
     4081 taccacttaa caacagatgg gaccaaactt gagaagttat ttgtagagcc caaggatgcc
     4141 atttgtgcca tctcctgcca cccatatcaa cccctcattg ccatcgggag catctgtggg
     4201 atgatcaaag tgtggaatta tgaaaacaaa caatatcttt tcagcagggt ttttgagaag
     4261 gggcttggag tccagagtct gacctacaac cccgaaggag cccttcttgg agctggcttt
     4321 acagagggga cagtttacat tcttgatgca atgtctttag aaaatgaaag cccagagcct
     4381 ttcaaatatt ccagaaccag tgtgactcat ataagctttt cccatgactc ccagtatatg
     4441 gcaactgctg atagaagttt tactgtggct gtttacatgc tggtggtcag aaatggacag
     4501 agggtctggg agtacttagc aagacttcgc tctcatcgca aaagcattcg aagtctcctg
     4561 tttggggttt acctggacag caatgagcct agactgctga gccttgggac agacaggctc
     4621 ttgatagagt atgatcttct caggagctac aaagaccacc tggaagtcct ggacattcac
     4681 cacaccgacc agggctgcta tcccacctgc atggtctggt acccaccact caccagggaa
     4741 ctcttcctgc ttatttgcaa cagtggctac aaagtgaagc tttttaatgc tactaccaaa
     4801 atgtgcagaa agacgcttct ggggccagct tatggttccc ctattgagca gacacaagtc
     4861 ctcccagtga gaagcatggc ggagctacag aaacgctact tggtgtttat taacagagac
     4921 aaggtgggac ttcagatctt accagttgac ggcaatccac ataagacatc tgctattgtt
     4981 tgccacccga acggggtggc cggcatggcc gtttcctatg atggctgcta cgccttcact
     5041 gcgggagggc acgatcgctc ggtggtgcag tggaaaatca ccttaagtgt cctggaggca
     5101 gcggtttctc ttgggggtga agacttgacc ccattctatg gtctgctgtc tggtggccgg
     5161 gaaggaaaat tctacaggga gctagaagac tacttctact attctcagct ccgcagtcaa
     5221 ggcatcgaca caatggagac cagaaaggtg tcagaacaca tttgcctgtc agagcttcct
     5281 tttgtcatga gagcaattgg cttttaccca tctgaagaga agattgatga tatatttaac
     5341 gaaatcaaat ttggtgaata tgtggacact ggaaagctaa tcgacaagat caacttacca
     5401 gatttcctaa aagtgtacct taaccacaag ccaccttttg gtaacaccat gagtggcatc
     5461 cacaagagct ttgaggtgct cggttatacc aactccaaag ggaaaaaggc cattcgaaga
     5521 gaggacttcc tgagactgct cgttactaaa ggtgagcata tgacggagga ggagatgttg
     5581 gattgctttg cttcactgtt tggcctgaat cccgagggat ggaaatccga gcctgcaacc
     5641 tgctccgtca aaggttcaga aatttgcctt gaagaagaac ttccagacga aatcactgca
     5701 gaaatattcg cgactgaaat tcttggctta accatttcag aagattccgg ccaggatggt
     5761 cagggatccg actacaaaga ccatgacggt gattataaag atcatgacat cgactacaag
     5821 gatgacgatg acaagtagcg gccgcgaagg atctgcgatc gctccggtgc ccgtcagtgg
     5881 gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacg
     5941 ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc
     6001 ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt
     6061 tttcgcaacg ggtttgccgc cagaacacag ctgaagcttc gaggggctcg catctctcct
     6121 tcacgcgccc gccgccctac ctgaggccgc catccacgcc ggttgagtcg cgttctgccg
     6181 cctcccgcct gtggtgcctc ctgaactgcg tccgccgtct aggtaagttt aaagctcagg
     6241 tcgagaccgg gcctttgtcc ggcgctccct tggagcctac ctagactcag ccggctctcc
     6301 acgctttgcc tgaccctgct tgctcaactc tacgtctttg tttcgttttc tgttctgcgc
     6361 cgttacagat ccaagctgtg accggcgcct acgctagacg ccaccatgga gagcgacgag
     6421 agcggcctgc ccgccatgga gatcgagtgc cgcatcaccg gcaccctgaa cggcgtggag
     6481 ttcgagctgg tgggcggcgg agagggcacc cccaagcagg gccgcatgac caacaagatg
     6541 aagagcacca aaggcgccct gaccttcagc ccctacctgc tgagccacgt gatgggctac
     6601 ggcttctacc acttcggcac ctaccccagc ggctacgaga accccttcct gcacgccatc
     6661 aacaacggcg gctacaccaa cacccgcatc gagaagtacg aggacggcgg cgtgctgcac
     6721 gtgagcttca gctaccgcta cgaggccggc cgcgtgatcg gcgacttcaa ggtggtgggc
     6781 accggcttcc ccgaggacag cgtgatcttc accgacaaga tcatccgcag caacgccacc
     6841 gtggagcacc tgcaccccat gggcgataac gtgctggtgg gcagcttcgc ccgcaccttc
     6901 agcctgcgcg acggcggcta ctacagcttc gtggtggaca gccacatgca cttcaagagc
     6961 gccatccacc ccagcatcct gcagaacggg ggccccatgt tcgccttccg ccgcgtggag
     7021 gagctgcaca gcaacaccga gctgggcatc gtggagtacc agcacgcctt caagaccccc
     7081 atcgccttcg ccagatcccg cgctcagtcg tccaattctg ccgtggacgg caccgccgga
     7141 cccggctcca ccggatctcg cgagggcaga ggaagtcttc taacatgcgg tgacgtggag
     7201 gagaatcccg gccctatgac cgagtacaag cccacggtgc gcctcgccac ccgcgacgac
     7261 gtccccaggg ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc cacgcgccac
     7321 accgtcgatc cggaccgcca catcgagcgg gtcaccgagc tgcaagaact cttcctcacg
     7381 cgcgtcgggc tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc ggtggcggtc
     7441 tggaccacgc cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg
     7501 gccgagttga gcggttcccg gctggccgcg cagcaacaga tggaaggcct cctggcgccg
     7561 caccggccca aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag
     7621 ggcaagggtc tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg
     7681 gtgcccgcct tcctggagac ctccgcgccc cgcaacctcc ccttctacga gcggctcggc
     7741 ttcaccgtca ccgccgacgt cgaggtgccc gaaggaccgc gcacctggtg catgacccgc
     7801 aagcccggtg cctgaaatca acctctggat tacaaaattt gtgaaagatt gactggtatt
     7861 cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat
     7921 gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct
     7981 ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct
     8041 gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc
     8101 gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg
     8161 acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc
     8221 tttccttggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac
     8281 gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg
     8341 cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc
     8401 ccgcctggta cctttaagac caatgactta caaggcagct gtagatctta gccacttttt
     8461 aaaagaaaag gggggactgg aagggctaat tcactcccaa cgaagataag atctgctttt
     8521 tgcttgtact gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact
     8581 agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc
     8641 ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa
     8701 aatctctagc agtagtagtt catgtcatct tattattcag tatttataac ttgcaaagaa
     8761 atgaatatca gagagtgaga ggaacttgtt tattgcagct tataatggtt acaaataaag
     8821 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
     8881 gtccaaactc atcaatgtat cttatcatgt ctggctctag ctatcccgcc cctaactccg
     8941 cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt
     9001 ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga
     9061 ggaggctttt ttggaggcct agacttttgc agagaccaaa ttcgtaatca tgtcatagct
     9121 gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat
     9181 aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc
     9241 actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg
     9301 cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct
     9361 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
     9421 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
     9481 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
     9541 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
     9601 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
     9661 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
     9721 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
     9781 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
     9841 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
     9901 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt
     9961 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
    10021 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
    10081 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
    10141 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
    10201 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac
    10261 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt
    10321 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt
    10381 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt
    10441 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc
    10501 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa
    10561 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg
    10621 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt
    10681 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc
    10741 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt
    10801 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg
    10861 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac
    10921 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc
    10981 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt
    11041 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg
    11101 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag
    11161 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa
    11221 acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtct aagaaaccat
    11281 tattatcatg acattaacct ataaaaatag gcgtatcacg aggccctttc gtctcgcgcg
    11341 tttcggtgat gacggtgaaa acctctgaca catgcagctc ccggagacgg tcacagcttg
    11401 tctgtaagcg gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg
    11461 gtgtcggggc tggcttaact atgcggcatc agagcagatt gtactgagag tgcaccatat
    11521 gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc gccattcgcc
    11581 attcaggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc tattacgcca
    11641 gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg gtaacgccag ggttttccca
    11701 gtcacgacgt tgtaaaacga cggccagtgc caagctg
//
