LOCUS       V032872                13956 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V032872
VERSION     V032872
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2303..8038
                     /label="CHD4(NM_001273)"
                     /note="CHD4(NM_001273)"
                     /gene="CHD4"
     CDS             8045..8110
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        8154..8365
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             8378..8646
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     CDS             8681..9388
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /transl_table=1
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
                     /label="mCherry"
     CDS             9398..9451
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             9452..10051
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    10052..10639
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             10713..10946
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    11018..11139
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      11158..11293
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(11331..11347)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    11355..11371
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(11379..11409)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    11424..11445
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(11733..12321)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(12492..13352)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(13353..13457)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     13931..13947
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc gaattcgcca ccatggcgtc gggcctgggc tccccgtccc cctgctcggc
     2341 gggcagtgag gaggaggata tggatgcact tttgaacaac agcctgcccc caccccaccc
     2401 agaaaatgaa gaggacccag aagaggattt gtcagaaaca gagactccaa agctcaagaa
     2461 gaagaaaaag cctaagaaac ctcgggaccc taaaatccct aagagcaagc gccaaaaaaa
     2521 ggagcgtatg ctcttatgcc ggcagctggg ggacagctct ggggaggggc cagagtttgt
     2581 ggaggaggag gaagaggtgg ctctgcgctc agacagtgag ggcagcgact atactcctgg
     2641 caagaagaag aagaagaagc ttggacctaa gaaagagaag aagagcaaat ccaagcggaa
     2701 ggaggaggag gaggaggagg atgatgatga tgattcaaag gagcctaaat catctgctca
     2761 gctcctggaa gactggggca tggaagacat tgaccacgtg ttctcagagg aggattatcg
     2821 aaccctcacc aactacaagg ccttcagcca gtttgtcaga cccctcattg ctgccaaaaa
     2881 tcccaagatt gctgtctcca agatgatgat ggttttgggt gcaaaatggc gggagttcag
     2941 taccaataac cccttcaaag gcagttctgg ggcatcagtg gcagctgcgg cagcagcagc
     3001 ggtagctgtg gtggagagca tggtgacagc cactgaggtt gcaccaccac ctccccctgt
     3061 ggaggtgcct atccgcaagg ccaagaccaa ggagggcaaa ggtcccaatg ctcggaggaa
     3121 gcccaagggc agccctcgtg tacctgatgc caagaagcct aaacccaaga aagtagctcc
     3181 cctgaaaatc aagctgggag gttttggttc caagcgtaag agatcctcga gtgaggatga
     3241 tgacttagat gtggaatctg acttcgatga tgccagtatc aatagctatt ctgtttctga
     3301 tggttccacc agccgtagta gccgcagccg caagaaactc cgaaccacta aaaagaaaaa
     3361 gaaaggcgag gaggaggtga ctgctgtgga tggttatgag acagaccacc aggactattg
     3421 cgaggtgtgc cagcaaggcg gtgagatcat cctgtgtgat acctgtcccc gtgcttacca
     3481 catggtctgc ctggatcccg acatggagaa ggctcccgag ggcaagtgga gctgcccaca
     3541 ctgcgagaag gaaggcatcc agtgggaagc taaagaggac aattcggagg gtgaggagat
     3601 cctggaagag gttgggggag acctcgaaga ggaggatgac caccatatgg aattctgtcg
     3661 ggtctgcaag gatggtgggg aactgctctg ctgtgatacc tgtccttctt cctaccacat
     3721 ccactgcctg aatcccccac ttccagagat ccccaacggt gaatggctct gtccccgttg
     3781 tacgtgtcca gctctgaagg gcaaagtgca gaagatccta atctggaagt ggggtcagcc
     3841 accatctccc acaccagtgc ctcggcctcc agatgctgat cccaacacgc cctccccaaa
     3901 gcccttggag gggcggccag agcggcagtt ctttgtgaaa tggcaaggca tgtcttactg
     3961 gcactgctcc tgggtttctg aactgcagct ggagctgcac tgtcaggtga tgttccgaaa
     4021 ctatcagcgg aagaatgata tggatgagcc accttctggg gactttggtg gtgatgaaga
     4081 gaaaagccga aagcgaaaga acaaggaccc taaatttgca gagatggagg aacgcttcta
     4141 tcgctatggg ataaaacccg agtggatgat gatccaccga atcctcaacc acagtgtgga
     4201 caagaagggc cacgtccact acttgatcaa gtggcgggac ttaccttacg atcaggcttc
     4261 ttgggagagt gaggatgtgg agatccagga ttacgacctg ttcaagcaga gctattggaa
     4321 tcacagggag ttaatgaggg gtgaggaagg ccgaccaggc aagaagctca agaaggtgaa
     4381 gcttcggaag ttggagaggc ctccagaaac gccaacagtt gatccaacag tgaagtatga
     4441 gcgacagcca gagtacctgg atgctacagg tggaaccctg cacccctatc aaatggaggg
     4501 cctgaattgg ttgcgcttct cctgggctca gggcactgac accatcttgg ctgatgagat
     4561 gggccttggg aaaactgtac agacagcagt cttcctgtat tccctttaca aggagggtca
     4621 ttccaaaggc cccttcctag tgagcgcccc tctttctacc atcatcaact gggagcggga
     4681 gtttgaaatg tgggctccag acatgtatgt cgtaacctat gtgggtgaca aggacagccg
     4741 tgccatcatc cgagagaatg agttctcctt tgaagacaat gccattcgtg gtggcaagaa
     4801 ggcctcccgc atgaagaaag aggcatctgt gaaattccat gtgctgctga catcctatga
     4861 attgatcacc attgacatgg ctattttggg ctctattgat tgggcctgcc tcatcgtgga
     4921 tgaagcccat cggctgaaga acaatcagtc taagttcttc cgggtattga atggttactc
     4981 actccagcac aagctgttgc tgactgggac accattacaa aacaatctgg aagagttgtt
     5041 tcatctgctc aactttctca cccccgagag gttccacaat ttggaaggtt ttttggagga
     5101 gtttgctgac attgccaagg aggaccagat aaaaaaactg catgacatgc tggggccgca
     5161 catgttgcgg cggctcaaag ccgatgtgtt caagaacatg ccctccaaga cagaactaat
     5221 tgtgcgtgtg gagctgagcc ctatgcagaa gaaatactac aagtacatcc tcactcgaaa
     5281 ttttgaagca ctcaatgccc gaggtggtgg caaccaggtg tctctgctga atgtggtgat
     5341 ggatcttaag aagtgctgca accatccata cctcttccct gtggctgcaa tggaagctcc
     5401 taagatgcct aatggcatgt atgatggcag tgccctaatc agagcatctg ggaaattatt
     5461 gctgctgcag aaaatgctca agaaccttaa ggagggtggg catcgtgtac tcatcttttc
     5521 ccagatgacc aagatgctag acctgctaga ggatttcttg gaacatgaag gttataaata
     5581 cgaacgcatc gatggtggaa tcactgggaa catgcggcaa gaggccattg accgcttcaa
     5641 tgcaccgggt gctcagcagt tctgcttctt gctttccact cgagctgggg gccttggaat
     5701 caatctggcc actgctgaca cagttattat ctatgactct gactggaacc cccataatga
     5761 cattcaggcc tttagcagag ctcaccggat tgggcaaaat aaaaaggtaa tgatctaccg
     5821 gtttgtgacc cgtgcgtcag tggaggagcg catcacgcag gtggcaaaga agaaaatgat
     5881 gctgacgcat ctagtggtgc ggcctgggct gggctccaag actggatcta tgtccaaaca
     5941 ggagcttgat gatatcctca aatttggcac tgaggaacta ttcaaggatg aagccactga
     6001 tggaggagga gacaacaaag agggagaaga tagcagtgtt atccactacg atgataaggc
     6061 cattgaacgg ctgctagacc gtaaccagga tgagactgaa gacacagaat tgcagggcat
     6121 gaatgaatat ttgagctcat tcaaagtggc ccagtatgtg gtacgggaag aagaaatggg
     6181 ggaggaagag gaggtagaac gggaaatcat taaacaggaa gaaagtgtgg atcctgacta
     6241 ctgggagaaa ttgctgcggc accattatga gcagcagcaa gaagatctag cccgaaatct
     6301 gggcaaagga aaaagaatcc gtaaacaggt caactacaat gatggctccc aggaggaccg
     6361 agattggcag gacgaccagt ccgacaacca gtccgattac tcagtggctt cagaggaagg
     6421 tgatgaagac tttgatgaac gttcagaagc tccccgtagg cccagtcgta agggcctgcg
     6481 gaatgataaa gataagccat tgcctcctct gttggcccgt gttggtggga atattgaagt
     6541 acttggtttt aatgctcgtc agcgaaaagc ctttcttaat gcaattatgc gatatggtat
     6601 gccacctcag gatgctttta ctacccagtg gcttgtaaga gacctgcgag gcaaatcaga
     6661 gaaagagttc aaggcatatg tctctctttt catgcggcat ttatgtgagc cgggggcaga
     6721 tggggctgag acctttgctg atggtgtccc ccgagaaggc ctgtctcgcc agcatgtcct
     6781 tactagaatt ggtgttatgt ctttgattcg caagaaggtt caggagtttg aacatgttaa
     6841 tgggcgctgg agcatgcctg aactggctga ggtggaggaa aacaagaaga tgtcccagcc
     6901 agggtcaccc tccccaaaaa ctcctacacc ctccactcca ggggacacgc agcccaacac
     6961 tcctgcacct gtcccacctg ctgaagatgg gataaaaata gaggaaaata gcctcaaaga
     7021 agaagagagc atagaaggag aaaaggaggt taaatctaca gcccctgaga ctgccattga
     7081 gtgtacacag gcccctgccc ctgcctcaga ggatgaaaag gtcgttgttg aaccccctga
     7141 gggagaggag aaagtggaaa aggcagaggt gaaggagaga acagaggaac ctatggagac
     7201 agagcccaaa ggtgctgctg atgtagagaa ggtggaggaa aagtcagcaa tagatctgac
     7261 ccctattgtg gtagaagaca aagaagagaa gaaagaagaa gaagagaaaa aagaggtgat
     7321 gcttcagaat ggagagaccc ccaaggacct gaatgatgag aaacagaaga aaaatattaa
     7381 acaacgtttc atgtttaaca ttgcagatgg tggttttact gagttgcact ccctttggca
     7441 gaatgaagag cgggcagcca cagttaccaa gaagacttat gagatctggc atcgacggca
     7501 tgactactgg ctgctagccg gcattataaa ccatggctat gcccggtggc aagacatcca
     7561 gaatgaccca cgctatgcca tcctcaatga gcctttcaag ggtgaaatga accgtggcaa
     7621 tttcttagag atcaagaata aatttctagc tcgaaggttt aagctcttag aacaagctct
     7681 ggtgattgag gaacagctgc gccgggctgc ttacttgaac atgtcagaag acccttctca
     7741 cccttccatg gccctcaaca cccgctttgc tgaggtggag tgtttggcgg aaagtcatca
     7801 gcacctgtcc aaggagtcaa tggcaggaaa caagccagcc aatgcagtcc tgcacaaagt
     7861 tctgaaacag ctggaagaac tgctgagtga catgaaagct gatgtgactc gactcccagc
     7921 taccattgcc cgaattcccc cagttgctgt gaggttacag atgtcagagc gtaacattct
     7981 cagccgcctg gcaaaccggg cacccgaacc taccccacag caggtagccc agcagcaggg
     8041 atccgactac aaagaccatg acggtgatta taaagatcat gacatcgact acaaggatga
     8101 cgatgacaag tagcggccgc gaaggatctg cgatcgctcc ggtgcccgtc agtgggcaga
     8161 gcgcacatcg cccacagtcc ccgagaagtt ggggggaggg gtcggcaatt gaacgggtgc
     8221 ctagagaagg tggcgcgggg taaactggga aagtgatgtc gtgtactggc tccgcctttt
     8281 tcccgagggt gggggagaac cgtatataag tgcagtagtc gccgtgaacg ttctttttcg
     8341 caacgggttt gccgccagaa cacagctgaa gcttcgaggg gctcgcatct ctccttcacg
     8401 cgcccgccgc cctacctgag gccgccatcc acgccggttg agtcgcgttc tgccgcctcc
     8461 cgcctgtggt gcctcctgaa ctgcgtccgc cgtctaggta agtttaaagc tcaggtcgag
     8521 accgggcctt tgtccggcgc tcccttggag cctacctaga ctcagccggc tctccacgct
     8581 ttgcctgacc ctgcttgctc aactctacgt ctttgtttcg ttttctgttc tgcgccgtta
     8641 cagatccaag ctgtgaccgg cgcctacgct agacgccacc atggtgagca agggcgagga
     8701 ggataacatg gccatcatca aggagttcat gcgcttcaag gtgcacatgg agggctccgt
     8761 gaacggccac gagttcgaga tcgagggcga gggcgagggc cgcccctacg agggcaccca
     8821 gaccgccaag ctgaaggtga ccaagggtgg ccccctgccc ttcgcctggg acatcctgtc
     8881 ccctcagttc atgtacggct ccaaggccta cgtgaagcac cccgccgaca tccccgacta
     8941 cttgaagctg tccttccccg agggcttcaa gtgggagcgc gtgatgaact tcgaggacgg
     9001 cggcgtggtg accgtgaccc aggactcctc cctgcaggac ggcgagttca tctacaaggt
     9061 gaagctgcgc ggcaccaact tcccctccga cggccccgta atgcagaaga agaccatggg
     9121 ctgggaggcc tcctccgagc ggatgtaccc cgaggacggc gccctgaagg gcgagatcaa
     9181 gcagaggctg aagctgaagg acggcggcca ctacgacgct gaggtcaaga ccacctacaa
     9241 ggccaagaag cccgtgcagc tgcccggcgc ctacaacgtc aacatcaagt tggacatcac
     9301 ctcccacaac gaggactaca ccatcgtgga acagtacgaa cgcgccgagg gccgccactc
     9361 caccggcggc atggacgagc tgtacaaggg atctcgcgag ggcagaggaa gtcttctaac
     9421 atgcggtgac gtggaggaga atcccggccc tatgaccgag tacaagccca cggtgcgcct
     9481 cgccacccgc gacgacgtcc ccagggccgt acgcaccctc gccgccgcgt tcgccgacta
     9541 ccccgccacg cgccacaccg tcgatccgga ccgccacatc gagcgggtca ccgagctgca
     9601 agaactcttc ctcacgcgcg tcgggctcga catcggcaag gtgtgggtcg cggacgacgg
     9661 cgccgcggtg gcggtctgga ccacgccgga gagcgtcgaa gcgggggcgg tgttcgccga
     9721 gatcggcccg cgcatggccg agttgagcgg ttcccggctg gccgcgcagc aacagatgga
     9781 aggcctcctg gcgccgcacc ggcccaagga gcccgcgtgg ttcctggcca ccgtcggcgt
     9841 ctcgcccgac caccagggca agggtctggg cagcgccgtc gtgctccccg gagtggaggc
     9901 ggccgagcgc gccggggtgc ccgccttcct ggagacctcc gcgccccgca acctcccctt
     9961 ctacgagcgg ctcggcttca ccgtcaccgc cgacgtcgag gtgcccgaag gaccgcgcac
    10021 ctggtgcatg acccgcaagc ccggtgcctg aaatcaacct ctggattaca aaatttgtga
    10081 aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt
    10141 aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa
    10201 atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt
    10261 gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct
    10321 cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg
    10381 ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc
    10441 ggggaagctg acgtcctttc catggctgct cgcctgtgtt gccacctgga ttctgcgcgg
    10501 gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct
    10561 gctgccggct ctgcggcctc ttccgcgtct ccgccttcgc cctcagacga gtcggatctc
    10621 cctttggccg cctccccgcc tggtaccttt aagaccaatg acttacaagg cagctgtaga
    10681 tcttagccac tttttaaaag aaaagggggg actggaaggg ctaattcact cccaacgaaa
    10741 ataagatctg ctttttgctt gtactgggtc tctctggtta gaccagatct gagcctggga
    10801 gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct
    10861 tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt
    10921 ttagtcagtg tggaaaatct ctagcagtag tagttcatgt catcttatta ttcagtattt
    10981 ataacttgca aagaaatgaa tatcagagag tgagaggaac ttgtttattg cagcttataa
    11041 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
    11101 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctggc tctagctatc
    11161 ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt
    11221 atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc
    11281 ttttttggag gcctagactt ttgcagagac ggcccaaatt cgtaatcatg gtcatagctg
    11341 tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata
    11401 aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca
    11461 ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc
    11521 gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg
    11581 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta
    11641 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc
    11701 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
    11761 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
    11821 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
    11881 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
    11941 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
    12001 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
    12061 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
    12121 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta
    12181 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
    12241 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
    12301 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
    12361 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc
    12421 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
    12481 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt
    12541 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta
    12601 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta
    12661 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
    12721 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat
    12781 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt
    12841 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg
    12901 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca
    12961 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta
    13021 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg
    13081 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact
    13141 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg
    13201 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt
    13261 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga
    13321 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc
    13381 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
    13441 caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta agaaaccatt
    13501 attatcatga cattaaccta taaaaatagg cgtatcacga ggccctttcg tctcgcgcgt
    13561 ttcggtgatg acggtgaaaa cctctgacac atgcagctcc cggagacggt cacagcttgt
    13621 ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg
    13681 tgtcggggct ggcttaacta tgcggcatca gagcagattg tactgagagt gcaccatatg
    13741 cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc gcatcaggcg ccattcgcca
    13801 ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct attacgccag
    13861 ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg gttttcccag
    13921 tcacgacgtt gtaaaacgac ggccagtgcc aagctg
//
