LOCUS       V044899                 9680 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V044899
VERSION     V044899
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1920
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2010..2213
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2302..3747
                     /label="CHRNA1(NM_001039523)"
                     /note="CHRNA1(NM_001039523)"
                     /gene="CHRNA1"
     CDS             3784..3849
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     CDS             3850..3906
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from porcine teschovirus-1
                     polyprotein"
                     /transl_table=1
                     /translation="ATNFSLLKQAGDVEENPGP"
                     /label="P2A"
     CDS             3907..4602
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="red fluorescent protein from Entacmaea
                     quadricolor"
                     /transl_table=1
                     /translation="MSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMKIKV
                     VEGGPLPFAFDILATSFMYGSKAFINHTQGIPDFFKQSFPEGFTWERITTYEDGGVLTA
                     TQDTSFQNGCIIYNVKINGVNFPSNGPVMQKKTRGWEANTEMLYPADGGLRGHSQMALK
                     LVGGGYLHCSFKTTYRSKKPAKNLKMPGFHFVDHRLERIKEADKETYVEQHEMAVAKYC
                     DLPSKLGHR*"
                     /label="TurboRFP"
     promoter        4650..4861
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             4874..5142
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     CDS             5169..5768
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    5775..6363
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             6437..6670
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    6742..6863
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      6882..7017
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(7055..7071)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    7079..7095
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(7103..7133)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    7148..7169
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(7457..8045)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(8216..9076)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(9077..9181)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     9655..9671
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt
     1921 atcgatacta gtattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
     1981 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
     2041 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
     2101 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
     2161 tgacgcaaat gggcggtagg cgtgtacggt gggaggttta tataagcaga gctcgtttag
     2221 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagattct
     2281 agagctagcg aattcgccac catggagccc tggcctctcc tcctgctctt tagcctttgc
     2341 tcagctggcc tcgtcctggg ctccgaacat gagacccgtc tggtggcaaa gctatttaaa
     2401 gactacagca gcgtggtgcg gccagtggaa gaccaccgcc aggtcgtgga ggtcaccgtg
     2461 ggcctgcagc tgatacagct catcaatgtg gatgaagtaa atcagatcgt gacaaccaat
     2521 gtgcgtctga aacagggtga catggtagat ctgccacgcc ccagctgcgt gactttggga
     2581 gttcctttgt tttctcatct gcagaatgag caatgggtgg attacaacct aaaatggaat
     2641 ccagatgact atggcggtgt gaaaaaaatt cacattcctt cagaaaagat ctggcgccca
     2701 gaccttgttc tctataacaa tgcagatggt gactttgcta ttgtcaagtt caccaaagtg
     2761 ctcctgcagt acactggcca catcacgtgg acacctccag ccatctttaa aagctactgt
     2821 gagatcatcg tcacccactt tccctttgat gaacagaact gcagcatgaa gctgggcacc
     2881 tggacctacg acggctctgt cgtggccatc aacccggaaa gcgaccagcc agacctgagc
     2941 aacttcatgg agagcgggga gtgggtgatc aaggagtccc ggggctggaa gcactccgtg
     3001 acctattcct gctgccccga caccccctac ctggacatca cctaccactt cgtcatgcag
     3061 cgcctgcccc tctacttcat cgtcaacgtc atcatcccct gcctgctctt ctccttctta
     3121 actggcctgg tattctacct gcccacagac tcaggggaga agatgactct gagcatctct
     3181 gtcttactgt ctttgactgt gttccttctg gtcatcgtgg agctgatccc ctccacgtcc
     3241 agtgctgtgc ccttgattgg aaaatacatg ctgttcacca tggtgttcgt cattgcctcc
     3301 atcatcatca ctgtcatcgt catcaacaca caccaccgct cacccagcac ccatgtcatg
     3361 cccaactggg tgcggaaggt ttttatcgac actatcccaa atatcatgtt tttctccaca
     3421 atgaaaagac catccagaga aaagcaagac aaaaagattt ttacagaaga cattgatatc
     3481 tctgacattt ctggaaagcc agggcctcca cccatgggct tccactctcc cctgatcaaa
     3541 caccccgagg tgaaaagtgc catcgagggc atcaagtaca tcgcagagac catgaagtca
     3601 gaccaggagt ctaacaatgc ggcggcagag tggaagtacg ttgcaatggt gatggaccac
     3661 atactcctcg gagtcttcat gcttgtttgc atcatcggaa ccctagccgt gtttgcaggt
     3721 cgactcattg aattaaatca gcaaggaggt ggaggcggat caggaggcgg tggatctctc
     3781 gaggactaca aagaccatga cggtgattat aaagatcatg acatcgacta caaggatgac
     3841 gatgacaagg ctactaactt cagcctgctg aagcaggctg gagacgtgga ggagaaccct
     3901 ggacctatga gcgagctgat caaggagaac atgcacatga agctgtacat ggagggcacc
     3961 gtgaacaacc accacttcaa gtgcacatcc gagggcgaag gcaagcccta cgagggcacc
     4021 cagaccatga agatcaaggt ggtcgagggc ggccctctcc ccttcgcctt cgacatcctg
     4081 gctaccagct tcatgtacgg cagcaaagcc ttcatcaacc acacccaggg catccccgac
     4141 ttctttaagc agtccttccc tgagggcttc acatgggaga gaatcaccac atacgaagac
     4201 gggggcgtgc tgaccgctac ccaggacacc agcttccaga acggctgcat catctacaac
     4261 gtcaagatca acggggtgaa cttcccatcc aacggccctg tgatgcagaa gaaaacacgc
     4321 ggctgggagg ccaacaccga gatgctgtac cccgctgacg gcggcctgag aggccacagc
     4381 cagatggccc tgaagctcgt gggcgggggc tacctgcact gctccttcaa gaccacatac
     4441 agatccaaga aacccgctaa gaacctcaag atgcccggct tccacttcgt ggaccacaga
     4501 ctggaaagaa tcaaggaggc cgacaaagag acctacgtcg agcagcacga gatggctgtg
     4561 gccaagtact gcgacctccc tagcaaactg gggcacagat gaggatccgc ggccgcgaag
     4621 gatctgcgat cgctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga
     4681 gaagttgggg ggaggggtcg gcaattgaac gggtgcctag agaaggtggc gcggggtaaa
     4741 ctgggaaagt gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta
     4801 tataagtgca gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca
     4861 gctgaagctt cgaggggctc gcatctctcc ttcacgcgcc cgccgcccta cctgaggccg
     4921 ccatccacgc cggttgagtc gcgttctgcc gcctcccgcc tgtggtgcct cctgaactgc
     4981 gtccgccgtc taggtaagtt taaagctcag gtcgagaccg ggcctttgtc cggcgctccc
     5041 ttggagccta cctagactca gccggctctc cacgctttgc ctgaccctgc ttgctcaact
     5101 ctacgtcttt gtttcgtttt ctgttctgcg ccgttacaga tccaagctgt gaccggcgcc
     5161 tacgctagat gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac gacgtccccc
     5221 gggccgtacg caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg
     5281 acccggaccg ccacatcgag cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg
     5341 ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca
     5401 cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc atggccgagt
     5461 tgagcggttc ccggctggcc gcgcagcaac agatggaagg cctcctggcg ccgcaccggc
     5521 ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac cagggcaagg
     5581 gtctgggcag cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg
     5641 ccttcctgga gacctccgcg ccccgcaacc tccccttcta cgagcggctc ggcttcaccg
     5701 tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg
     5761 gtgcctgagt cgacaatcaa cctctggatt acaaaatttg tgaaagattg actggtattc
     5821 ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg
     5881 ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc
     5941 tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg
     6001 acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg
     6061 ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga
     6121 caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct
     6181 ttccttggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg
     6241 tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc
     6301 ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc
     6361 cgcctggtac ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta
     6421 aaagaaaagg ggggactgga agggctaatt cactcccaac gaaaataaga tctgcttttt
     6481 gcttgtactg ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta
     6541 gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc
     6601 cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa
     6661 atctctagca gtagtagttc atgtcatctt attattcagt atttataact tgcaaagaaa
     6721 tgaatatcag agagtgagag gaacttgttt attgcagctt ataatggtta caaataaagc
     6781 aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg
     6841 tccaaactca tcaatgtatc ttatcatgtc tggctctagc tatcccgccc ctaactccgc
     6901 ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg
     6961 aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag
     7021 acttttgcag agacggccca aattcgtaat catggtcata gctgtttcct gtgtgaaatt
     7081 gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg
     7141 gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt
     7201 cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt
     7261 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
     7321 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
     7381 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
     7441 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
     7501 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     7561 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
     7621 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
     7681 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     7741 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
     7801 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
     7861 tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc
     7921 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
     7981 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
     8041 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
     8101 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
     8161 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc
     8221 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
     8281 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
     8341 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
     8401 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
     8461 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
     8521 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
     8581 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
     8641 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
     8701 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
     8761 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
     8821 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
     8881 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
     8941 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
     9001 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
     9061 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt
     9121 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc
     9181 gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac cattattatc atgacattaa
     9241 cctataaaaa taggcgtatc acgaggccct ttcgtctcgc gcgtttcggt gatgacggtg
     9301 aaaacctctg acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg
     9361 ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggctggctta
     9421 actatgcggc atcagagcag attgtactga gagtgcacca tatgcggtgt gaaataccgc
     9481 acagatgcgt aaggagaaaa taccgcatca ggcgccattc gccattcagg ctgcgcaact
     9541 gttgggaagg gcgatcggtg cgggcctctt cgctattacg ccagctggcg aaagggggat
     9601 gtgctgcaag gcgattaagt tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa
     9661 cgacggccag tgccaagctg
//
