LOCUS       V039580                10760 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V039580
VERSION     V039580
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2317..4854
                     /label="CLOCK(NM_004898)"
                     /note="CLOCK(NM_004898)"
                     /gene="CLOCK"
     promoter        4919..5130
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             5143..5411
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      5440..5449
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             5470..6132
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             6202..6255
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             6256..6855
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    6856..7443
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7517..7750
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    7822..7943
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      7962..8097
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(8135..8151)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8159..8175
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8183..8213)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8228..8249
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8537..9125)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9296..10156)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10157..10261)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     10735..10751
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc ctgatacgaa ctcggaattc gccaccatgt tgtttaccgt aagctgtagt
     2341 aaaatgagct cgattgttga cagagatgac agtagtattt ttgatgggtt ggtggaagaa
     2401 gatgacaagg acaaagcgaa aagagtatct agaaacaaat ctgaaaagaa acgtagagat
     2461 caatttaatg ttctcattaa agaactggga tccatgcttc ctggtaatgc tagaaagatg
     2521 gacaaatcta ctgttctgca gaaaagcatt gattttttac gaaaacataa agaaatcact
     2581 gcacagtcag atgctagtga aattcgacag gactggaaac ctacattcct tagtaatgaa
     2641 gagtttacac aattaatgtt agaggctctt gatggttttt ttttagcaat catgacagat
     2701 ggaagcataa tatatgtgtc tgagagtgta acttcattac ttgaacattt accatctgat
     2761 cttgtggatc aaagtatatt taattttatc ccagaagggg aacattcaga ggtttataaa
     2821 atactctcta ctcatctgct ggaaagtgat tcattaaccc cagaatattt aaaatcaaaa
     2881 aatcagttag aattctgttg tcacatgctg cgaggaacaa tagacccaaa ggagccatct
     2941 acctatgaat atgtaaaatt tataggaaat ttcaaatctt taaacagtgt atcctcttca
     3001 gcacacaatg gttttgaagg aactatacaa cgcacacata ggccatctta tgaagataga
     3061 gtttgttttg tagctactgt caggttagct acacctcagt tcatcaagga aatgtgcact
     3121 gttgaagaac ccaatgaaga gtttacatct agacatagtt tagaatggaa gtttctgttt
     3181 ctagatcaca gggcaccacc cataataggg tatttgccat ttgaagttct gggaacatca
     3241 ggctatgatt actatcatgt ggatgaccta gaaaatttgg caaaatgtca tgagcactta
     3301 atgcaatatg ggaaaggcaa atcatgttat tataggttcc tgactaaggg gcaacagtgg
     3361 atttggcttc agactcatta ttatatcact taccatcagt ggaattcaag gccagagttt
     3421 attgtttgta ctcacactgt agtaagttat gcagaagtta gggctgaaag acgacgagaa
     3481 cttggcattg aagagtctct tcctgagaca gctgctgaca aaagccaaga ttctgggtca
     3541 gataatcgta taaacacagt cagtctcaag gaagcattgg aaaggtttga tcacagccca
     3601 accccttctg cctcttctcg gagttcaaga aaatcatctc acacggccgt ctcagaccct
     3661 tcctcaacac caaccaagat cccgacggat acgagcactc cacccaggca gcatttacca
     3721 gctcatgaga agatggtgca aagaaggtca tcatttagta gtcagtccat aaattcccag
     3781 tctgttggtt catcattaac acagccagtg atgtctcaag ctacaaattt accaattcca
     3841 caaggcatgt cccagtttca gttttcagct caattaggag ccatgcaaca tctgaaagac
     3901 caattggaac aacggacacg catgatagaa gcaaatattc atcggcaaca agaagaacta
     3961 agaaaaattc aagaacaact tcagatggtc catggtcagg ggctgcagat gtttttgcaa
     4021 caatcaaatc ctgggttgaa ttttggttcc gttcaacttt cttctggaaa ttcatctaat
     4081 atccagcaac ttgcacctat aaatatgcaa ggccaagttg ttcctactaa ccagattcaa
     4141 agtggaatga atactggaca cattggcaca actcagcaca tgatacaaca acagacttta
     4201 cagagtacat caactcagag tcaacaaaat gtactgagtg ggcacagtca gcaaacatct
     4261 ctacccagtc agacacagag cactcttaca gccccactgt ataacactat ggtgatttct
     4321 cagcctgcag ccggaagcat ggtccagatt ccatctagta tgccacaaaa cagcacccag
     4381 agtgctgcag taactacatt cactcaggac aggcagataa gattttctca aggtcaacaa
     4441 cttgtgacca aattagtgac tgctcctgta gcttgtgggg cagtcatggt acctagtact
     4501 atgcttatgg gccaggtggt gactgcatat cctacttttg ctacacaaca gcaacagtca
     4561 cagacattgt cagtaacgca gcagcagcag cagcagagct cccaggagca gcagctcact
     4621 tcagttcagc aaccatctca ggctcagctg acccagccac cgcaacaatt tttacagact
     4681 tctaggttgc tccatgggaa tccctcaact caactcattc tctctgctgc atttcctcta
     4741 caacagagca ccttccctca gtcacatcac cagcaacatc agtctcagca acagcagcaa
     4801 ctcagccggc acaggactga cagcttgccc gacccttcca aggttcaacc acagtaggga
     4861 tccctgcgat atccagcgcg gccgcgaagg atctgcgatc gctccggtgc ccgtcagtgg
     4921 gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacg
     4981 ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc
     5041 ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt
     5101 tttcgcaacg ggtttgccgc cagaacacag ctgaagcttc gaggggctcg catctctcct
     5161 tcacgcgccc gccgccctac ctgaggccgc catccacgcc ggttgagtcg cgttctgccg
     5221 cctcccgcct gtggtgcctc ctgaactgcg tccgccgtct aggtaagttt aaagctcagg
     5281 tcgagaccgg gcctttgtcc ggcgctccct tggagcctac ctagactcag ccggctctcc
     5341 acgctttgcc tgaccctgct tgctcaactc tacgtctttg tttcgttttc tgttctgcgc
     5401 cgttacagat ccaagctgtg accggcgcct acgctagacg ccaccatgga gagcgacgag
     5461 agcggcctgc ccgccatgga gatcgagtgc cgcatcaccg gcaccctgaa cggcgtggag
     5521 ttcgagctgg tgggcggcgg agagggcacc cccaagcagg gccgcatgac caacaagatg
     5581 aagagcacca aaggcgccct gaccttcagc ccctacctgc tgagccacgt gatgggctac
     5641 ggcttctacc acttcggcac ctaccccagc ggctacgaga accccttcct gcacgccatc
     5701 aacaacggcg gctacaccaa cacccgcatc gagaagtacg aggacggcgg cgtgctgcac
     5761 gtgagcttca gctaccgcta cgaggccggc cgcgtgatcg gcgacttcaa ggtggtgggc
     5821 accggcttcc ccgaggacag cgtgatcttc accgacaaga tcatccgcag caacgccacc
     5881 gtggagcacc tgcaccccat gggcgataac gtgctggtgg gcagcttcgc ccgcaccttc
     5941 agcctgcgcg acggcggcta ctacagcttc gtggtggaca gccacatgca cttcaagagc
     6001 gccatccacc ccagcatcct gcagaacggg ggccccatgt tcgccttccg ccgcgtggag
     6061 gagctgcaca gcaacaccga gctgggcatc gtggagtacc agcacgcctt caagaccccc
     6121 atcgccttcg ccagatcccg cgctcagtcg tccaattctg ccgtggacgg caccgccgga
     6181 cccggctcca ccggatctcg cgagggcaga ggaagtcttc taacatgcgg tgacgtggag
     6241 gagaatcccg gccctatgac cgagtacaag cccacggtgc gcctcgccac ccgcgacgac
     6301 gtccccaggg ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc cacgcgccac
     6361 accgtcgatc cggaccgcca catcgagcgg gtcaccgagc tgcaagaact cttcctcacg
     6421 cgcgtcgggc tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc ggtggcggtc
     6481 tggaccacgc cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg
     6541 gccgagttga gcggttcccg gctggccgcg cagcaacaga tggaaggcct cctggcgccg
     6601 caccggccca aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag
     6661 ggcaagggtc tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg
     6721 gtgcccgcct tcctggagac ctccgcgccc cgcaacctcc ccttctacga gcggctcggc
     6781 ttcaccgtca ccgccgacgt cgaggtgccc gaaggaccgc gcacctggtg catgacccgc
     6841 aagcccggtg cctgaaatca acctctggat tacaaaattt gtgaaagatt gactggtatt
     6901 cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat
     6961 gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct
     7021 ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct
     7081 gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc
     7141 gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg
     7201 acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa gctgacgtcc
     7261 tttccatggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac
     7321 gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg
     7381 cctcttccgc gtctccgcct tcgccctcag acgagtcgga tctccctttg gccgcctccc
     7441 cgcctggtac ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta
     7501 aaagaaaagg ggggactgga agggctaatt cactcccaac gaaaataaga tctgcttttt
     7561 gcttgtactg ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta
     7621 gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc
     7681 cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa
     7741 atctctagca gtagtagttc atgtcatctt attattcagt atttataact tgcaaagaaa
     7801 tgaatatcag agagtgagag gaacttgttt attgcagctt ataatggtta caaataaagc
     7861 aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg
     7921 tccaaactca tcaatgtatc ttatcatgtc tggctctagc tatcccgccc ctaactccgc
     7981 ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg
     8041 aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag
     8101 acttttgcag agacggccca aattcgtaat catggtcata gctgtttcct gtgtgaaatt
     8161 gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg
     8221 gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt
     8281 cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt
     8341 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
     8401 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
     8461 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
     8521 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
     8581 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     8641 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
     8701 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
     8761 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     8821 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
     8881 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
     8941 tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc
     9001 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
     9061 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
     9121 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
     9181 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
     9241 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc
     9301 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
     9361 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
     9421 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
     9481 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
     9541 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
     9601 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
     9661 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
     9721 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
     9781 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
     9841 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
     9901 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
     9961 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
    10021 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
    10081 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
    10141 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt
    10201 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc
    10261 gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac cattattatc atgacattaa
    10321 cctataaaaa taggcgtatc acgaggccct ttcgtctcgc gcgtttcggt gatgacggtg
    10381 aaaacctctg acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg
    10441 ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggctggctta
    10501 actatgcggc atcagagcag attgtactga gagtgcacca tatgcggtgt gaaataccgc
    10561 acagatgcgt aaggagaaaa taccgcatca ggcgccattc gccattcagg ctgcgcaact
    10621 gttgggaagg gcgatcggtg cgggcctctt cgctattacg ccagctggcg aaagggggat
    10681 gtgctgcaag gcgattaagt tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa
    10741 cgacggccag tgccaagctg
//
