LOCUS       V033062                11331 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V033062
VERSION     V033062
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1798..1915
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2005..2208
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2297..5350
                     /label="CNTN1(NM_001843)"
                     /note="CNTN1(NM_001843)"
                     /gene="CNTN1"
     CDS             5357..5446
                     /codon_start=1
                     /product="3 tandem Myc epitope tags"
                     /transl_table=1
                     /translation="EQKLISEEDLEQKLISEEDLEQKLISEEDL"
                     /label="3xMyc"
     promoter        5490..5701
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             5714..5982
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      6011..6020
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             6041..6703
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             6773..6826
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             6827..7426
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    7427..8014
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             8088..8321
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    8393..8514
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      8533..8668
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(8706..8722)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8730..8746
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8754..8784)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8799..8820
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(9108..9696)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9867..10727)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10728..10832)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     11306..11322
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggttaacttt
     1801 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc
     1861 aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa attttatcga
     1921 tactagtatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac
     1981 gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga
     2041 tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg
     2101 ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg
     2161 caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctcg tttagtgaac
     2221 cgtcagatcg cctggagacg ccatccacgc tgttttgacc tccatagaag attctagagc
     2281 tagcgaattc gccaccatga aaatgtggtt gctggtcagt catcttgtga taatatctat
     2341 tactacctgt ttagcagagt ttacatggta tagaagatat ggtcatggag tttctgagga
     2401 agacaaagga tttggaccaa tttttgaaga gcagccaatc aataccattt atccagagga
     2461 atcactggaa ggaaaagtct cactcaactg tagggcacga gccagccctt tcccggttta
     2521 caaatggaga atgaataatg gggacgttga tctcacaagt gatcgataca gtatggtagg
     2581 aggaaacctt gttatcaaca accctgacaa acagaaagat gctggaatat actactgttt
     2641 agcatctaat aactacggga tggtcagaag cactgaagca accctgagct ttggatatct
     2701 tgatcctttc ccacctgagg aacgtcctga ggtcagagta aaagaaggga aaggaatggt
     2761 gcttctctgt gaccccccat accattttcc agatgatctt agctatcgct ggcttctaaa
     2821 tgaatttcct gtatttatca caatggataa acggcgattt gtgtctcaga caaatggcaa
     2881 tctctacatt gcaaatgttg aggcttccga caaaggcaat tattcctgct ttgtttccag
     2941 tccttctatt acaaagagcg tgttcagcaa attcatccca ctcattccaa tacctgaacg
     3001 aacaacaaaa ccatatcctg ctgatattgt agttcagttc aaggatgtat atgcattgat
     3061 gggccaaaat gtgaccttag aatgttttgc acttggaaat cctgttccgg atatccgatg
     3121 gcggaaggtt ctagaaccaa tgccaagcac tgctgagatt agcacctctg gggctgttct
     3181 taagatcttc aatattcagc tagaagatga aggcatctat gaatgtgagg ctgagaacat
     3241 tagaggaaag gataaacatc aagcaagaat ttatgttcaa gcattccctg agtgggtaga
     3301 acacatcaat gacacagagg tggacatagg cagtgatctc tactggcctt gtgtggccac
     3361 aggaaagccc atccctacaa tccgatggtt gaaaaatgga tatgcgtatc ataaagggga
     3421 attaagactg tatgatgtga cttttgaaaa tgccggaatg tatcagtgca tagctgaaaa
     3481 cacatatgga gccatttatg caaatgctga gttgaagatc ttggcgttgg ctccaacttt
     3541 tgaaatgaat cctatgaaga aaaagatcct ggctgctaaa ggtggaaggg tgataattga
     3601 atgcaaacct aaagctgcac cgaaaccaaa gttttcatgg agtaaaggga cagagtggct
     3661 tgtcaatagc agcagaatac tcatttggga agatggtagc ttggaaatca acaacattac
     3721 aaggaatgat ggaggtatct atacatgctt tgcagaaaat aacagaggga aagctaatag
     3781 cactggaacc cttgttatca cagatcctac gcgaattata ttggccccaa ttaatgccga
     3841 tatcacagtt ggagaaaacg ccaccatgca gtgtgctgcg tcctttgatc ctgccttgga
     3901 tctcacattt gtttggtcct tcaatggcta tgtgatcgat tttaacaaag agaatattca
     3961 ctaccagagg aattttatgc tggattccaa tggggaatta ctaatccgaa atgcgcagct
     4021 gaaacatgct ggaagataca catgcactgc ccagacaatt gtggacaatt cttcagcttc
     4081 agctgacctt gtagtgagag gccctccagg ccctccaggt ggtctgagaa tagaagacat
     4141 tagagccact tctgtggcac ttacttggag ccgtggttca gacaatcata gtcctatttc
     4201 taaatacact atccagacca agactattct ttcagatgac tggaaagatg caaagacaga
     4261 tcccccaatt attgaaggaa atatggaggc agcaagagca gtggacttaa tcccatggat
     4321 ggagtatgaa ttccgcgtgg tagcaaccaa tacactgggt agaggagagc ccagtatacc
     4381 atctaacaga attaaaacag acggtgctgc accaaatgtg gctccttcag atgtaggagg
     4441 tggaggtgga agaaacagag agctgaccat aacatgggcg cctttgtcaa gagaatacca
     4501 ctatggcaac aattttggtt acatagtggc atttaagcca tttgatggag aagaatggaa
     4561 aaaagtcaca gttactaatc ctgatactgg ccgatatgtc cataaagatg aaaccatgag
     4621 cccttccact gcatttcaag ttaaagtcaa ggccttcaac aacaaaggag atggacctta
     4681 cagcctagta gcagtcatta attcagcaca agacgctccc agtgaagccc caacagaagt
     4741 aggtgtaaaa gtcttatcat cttctgagat atctgttcat tgggaacatg ttttagaaaa
     4801 aatagtggaa agctatcaga ttcggtattg ggctgcccat gacaaagaag aagctgcaaa
     4861 cagagttcaa gtcaccagcc aagagtactc ggccaggctc gagaaccttc tgccagacac
     4921 ccagtatttt atagaagtcg gggcctgcaa tagtgcaggg tgtggacctc caagtgacat
     4981 gattgaggct ttcaccaaga aagcacctcc tagccagcct ccaaggatca tcagttcagt
     5041 aaggtctggt tcacgctata taatcacctg ggatcatgtc gttgcactat caaatgaatc
     5101 tacagtgacg ggatataagg tactctacag acctgatggc cagcatgatg gcaagctgta
     5161 ttcaactcac aaacactcca tagaagtccc aatccccaga gatggagaat acgttgtgga
     5221 ggttcgcgcg cacagtgatg gaggagatgg agtggtgtct caagtcaaaa tttcaggtgc
     5281 acccacccta tccccaagtc ttctcggctt actgctgcct gcctttggca tccttgtcta
     5341 cttggaattc ggatccgaac aaaaactaat atcagaggaa gatttggaac agaaactaat
     5401 cagtgaagaa gatttagagc aaaagttaat ttccgaggag gacttatagc ggccgcgaag
     5461 gatctgcgat cgctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga
     5521 gaagttgggg ggaggggtcg gcaattgaac gggtgcctag agaaggtggc gcggggtaaa
     5581 ctgggaaagt gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta
     5641 tataagtgca gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca
     5701 gctgaagctt cgaggggctc gcatctctcc ttcacgcgcc cgccgcccta cctgaggccg
     5761 ccatccacgc cggttgagtc gcgttctgcc gcctcccgcc tgtggtgcct cctgaactgc
     5821 gtccgccgtc taggtaagtt taaagctcag gtcgagaccg ggcctttgtc cggcgctccc
     5881 ttggagccta cctagactca gccggctctc cacgctttgc ctgaccctgc ttgctcaact
     5941 ctacgtcttt gtttcgtttt ctgttctgcg ccgttacaga tccaagctgt gaccggcgcc
     6001 tacgctagac gccaccatgg agagcgacga gagcggcctg cccgccatgg agatcgagtg
     6061 ccgcatcacc ggcaccctga acggcgtgga gttcgagctg gtgggcggcg gagagggcac
     6121 ccccaagcag ggccgcatga ccaacaagat gaagagcacc aaaggcgccc tgaccttcag
     6181 cccctacctg ctgagccacg tgatgggcta cggcttctac cacttcggca cctaccccag
     6241 cggctacgag aaccccttcc tgcacgccat caacaacggc ggctacacca acacccgcat
     6301 cgagaagtac gaggacggcg gcgtgctgca cgtgagcttc agctaccgct acgaggccgg
     6361 ccgcgtgatc ggcgacttca aggtggtggg caccggcttc cccgaggaca gcgtgatctt
     6421 caccgacaag atcatccgca gcaacgccac cgtggagcac ctgcacccca tgggcgataa
     6481 cgtgctggtg ggcagcttcg cccgcacctt cagcctgcgc gacggcggct actacagctt
     6541 cgtggtggac agccacatgc acttcaagag cgccatccac cccagcatcc tgcagaacgg
     6601 gggccccatg ttcgccttcc gccgcgtgga ggagctgcac agcaacaccg agctgggcat
     6661 cgtggagtac cagcacgcct tcaagacccc catcgccttc gccagatccc gcgctcagtc
     6721 gtccaattct gccgtggacg gcaccgccgg acccggctcc accggatctc gcgagggcag
     6781 aggaagtctt ctaacatgcg gtgacgtgga ggagaatccc ggccctatga ccgagtacaa
     6841 gcccacggtg cgcctcgcca cccgcgacga cgtccccagg gccgtacgca ccctcgccgc
     6901 cgcgttcgcc gactaccccg ccacgcgcca caccgtcgat ccggaccgcc acatcgagcg
     6961 ggtcaccgag ctgcaagaac tcttcctcac gcgcgtcggg ctcgacatcg gcaaggtgtg
     7021 ggtcgcggac gacggcgccg cggtggcggt ctggaccacg ccggagagcg tcgaagcggg
     7081 ggcggtgttc gccgagatcg gcccgcgcat ggccgagttg agcggttccc ggctggccgc
     7141 gcagcaacag atggaaggcc tcctggcgcc gcaccggccc aaggagcccg cgtggttcct
     7201 ggccaccgtc ggcgtctcgc ccgaccacca gggcaagggt ctgggcagcg ccgtcgtgct
     7261 ccccggagtg gaggcggccg agcgcgccgg ggtgcccgcc ttcctggaga cctccgcgcc
     7321 ccgcaacctc cccttctacg agcggctcgg cttcaccgtc accgccgacg tcgaggtgcc
     7381 cgaaggaccg cgcacctggt gcatgacccg caagcccggt gcctgaaatc aacctctgga
     7441 ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg
     7501 tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt
     7561 ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag
     7621 gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc
     7681 caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga
     7741 actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa
     7801 ttccgtggtg ttgtcgggga agctgacgtc ctttccatgg ctgctcgcct gtgttgccac
     7861 ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct
     7921 tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtctccgcc ttcgccctca
     7981 gacgagtcgg atctcccttt ggccgcctcc ccgcctggta cctttaagac caatgactta
     8041 caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg aagggctaat
     8101 tcactcccaa cgaaaataag atctgctttt tgcttgtact gggtctctct ggttagacca
     8161 gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag
     8221 cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag
     8281 atccctcaga cccttttagt cagtgtggaa aatctctagc agtagtagtt catgtcatct
     8341 tattattcag tatttataac ttgcaaagaa atgaatatca gagagtgaga ggaacttgtt
     8401 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc
     8461 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt
     8521 ctggctctag ctatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc
     8581 tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag
     8641 aagtagtgag gaggcttttt tggaggccta gacttttgca gagacggccc aaattcgtaa
     8701 tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata
     8761 cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta
     8821 attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa
     8881 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg
     8941 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
     9001 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
     9061 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
     9121 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
     9181 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
     9241 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
     9301 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
     9361 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
     9421 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
     9481 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
     9541 actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
     9601 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
     9661 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
     9721 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
     9781 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
     9841 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
     9901 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
     9961 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
    10021 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
    10081 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
    10141 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca
    10201 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
    10261 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
    10321 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
    10381 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
    10441 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg
    10501 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
    10561 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
    10621 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
    10681 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
    10741 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
    10801 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac
    10861 gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc
    10921 tttcgtctcg cgcgtttcgg tgatgacggt gaaaacctct gacacatgca gctcccggag
    10981 acggtcacag cttgtctgta agcggatgcc gggagcagac aagcccgtca gggcgcgtca
    11041 gcgggtgttg gcgggtgtcg gggctggctt aactatgcgg catcagagca gattgtactg
    11101 agagtgcacc atatgcggtg tgaaataccg cacagatgcg taaggagaaa ataccgcatc
    11161 aggcgccatt cgccattcag gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct
    11221 tcgctattac gccagctggc gaaaggggga tgtgctgcaa ggcgattaag ttgggtaacg
    11281 ccagggtttt cccagtcacg acgttgtaaa acgacggcca gtgccaagct g
//
