LOCUS       V025616                12879 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V025616
VERSION     V025616
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     sig_peptide     2320..2379
                     /codon_start=1
                     /product="leader sequence from mouse immunoglobulin&nbsp;κ
                     light chain"
                     /transl_table=1
                     /translation="METDTLLLWVLLLWVPGSTG"
                     /label="Igκ leader"
     CDS             2383..2400
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /transl_table=1
                     /translation="HHHHHH"
                     /label="6xHis"
     CDS             2401..2424
                     /codon_start=1
                     /product="Xpress™ epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /transl_table=1
                     /translation="DLY,DDDDK"
                     /label="Xpress™ tag"
     CDS             2503..6993
                     /label="COL17A1(NM_000494)"
                     /note="COL17A1(NM_000494)"
                     /gene="COL17A1"
     promoter        7038..7249
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             7262..7530
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      7559..7568
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             7589..8251
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             8321..8374
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             8375..8974
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    8975..9562
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             9636..9869
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    9941..10062
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      10081..10216
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(10254..10270)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    10278..10294
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(10302..10332)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    10347..10368
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(10656..11244)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(11415..12275)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(12276..12380)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     12854..12870
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc gaattcgaat ttaaatcgga tccgccacca tggagacaga cacactcctg
     2341 ctatgggtac tgctgctctg ggttccaggt tccactggtg accatcatca tcatcatcat
     2401 gatctgtacg acgatgacga taagtacggc cgcaagaaac gccgccagcg ccgccgcggt
     2461 ggaggcggaa gcggaggtgg cggatctggt ggaggcggaa gcatggatgt aaccaagaaa
     2521 aacaaacgag atggaactga agtcactgag agaattgtca ctgaaacagt aaccacaaga
     2581 cttacatcct taccaccaaa aggcgggacc agcaatggct atgctaaaac agcctctctt
     2641 ggtggaggga gccggctgga gaaacaaagc ctgactcatg gcagcagcgg ctacataaac
     2701 tcaactggaa gcacacgagg ccatgcctcc acctctagtt acaggagggc tcactcacct
     2761 gcctccactc tgcccaactc cccaggctca acctttgaaa ggaaaactca cgttacccgc
     2821 catgcgtatg aagggagctc cagtggcaac tcttctccgg agtaccctcg gaaggaattt
     2881 gcatcttctt caaccagagg acggagtcaa acacgagaga gtgaaattcg agttcgactg
     2941 cagagtgcgt ccccatccac ccgatggaca gaattggatg atgttaagcg tttgctcaag
     3001 gggagtcgat cggcaagtgt gagccccacc cggaattcct ccaacacact ccccatcccc
     3061 aagaaaggca ctgtggagac caaaattgtg acagcgagct cccagtcggt gtcaggcacc
     3121 tacgatgcaa cgatcctgga tgccaacctt ccctcccatg tgtggtcctc caccctgccc
     3181 gcggggtcct ccatggggac ctatcacaac aacatgacaa cccagagctc atccctcctc
     3241 aacaccaatg cctactctgc gggatcagtc ttcggagttc caaacaacat ggcgtcctgc
     3301 tcacccactt tgcaccctgg actcagcaca tcctcctcag tgtttggcat gcagaacaat
     3361 ctggccccca gcttgaccac cctgtcccat ggcaccacca ccacttccac agcatatggg
     3421 gtgaagaaaa acatgcccca gagtcctgcg gctgtgaaca ctggcgtttc cacctccgcc
     3481 gcctgcacca caagtgtgca gagcgatgac cttttgcaca aggactgcaa gttcctgatc
     3541 ctagagaaag acaacacacc tgccaagaag gagatggagc tgctcatcat gaccaaggac
     3601 agcgggaagg tctttacagc ctcccctgcc agcatcgctg caacttcttt ttcagaagac
     3661 accctaaaaa aagaaaagca agctgcctac aatgctgact caggcctaaa agccgaagct
     3721 aatggagacc tgaagactgt gtccacaaag ggcaagacca ccactgcaga tatccacagc
     3781 tacggcagca gtggtggtgg tggcagtgga ggaggtggcg gtgttggtgg cgctggcggc
     3841 ggcccttggg gaccagcgcc agcctggtgc ccctgcggct cctgctgcag ctggtggaag
     3901 tggctgctgg gcctgctgct cacctggctg ctactcctgg ggctgctctt cggcctcatt
     3961 gctctggcgg aggaggtgag gaagctgaag gcgcgtgtgg atgagctgga gaggatcagg
     4021 aggagcatac tgccctatgg ggacagcatg gatagaatag aaaaggaccg cctccagggc
     4081 atggcacccg cggcgggagc agacctggac aaaattgggc tgcacagtga cagccaggag
     4141 gagctctgga tgttcgtgag gaagaagcta atgatggaac aggaaaatgg aaatctccga
     4201 ggaagccctg gccctaaagg tgacatggga agtccaggcc ctaaaggaga tcgagggttc
     4261 cctgggactc caggtatccc tgggcccttg ggccacccag gtccacaagg accaaagggt
     4321 caaaaaggca gcgtgggaga tcctggcatg gaaggcccca tgggccagag agggcgagaa
     4381 ggccccatgg gacctcgtgg tgaggcaggg cctcctggat ctggagagaa aggggaaaga
     4441 ggggctgctg gtgaaccagg tcctcatggc ccacctggtg tcccaggttc tgtgggtccc
     4501 aaaggttcca gcggctctcc tggcccacag ggccctccag gtcctgtagg tctccaaggg
     4561 ctccgaggtg aagtaggact tcctggtgtc aaaggtgaca aaggaccaat gggaccacca
     4621 ggacccaaag gtgaccaggg tgagaaagga cctcgaggcc tcacaggcga gcctggcatg
     4681 agaggtttgc ctggtgctgt tggtgagccc ggggctaaag gagcaatggg tcctgctggc
     4741 ccagacggac accaaggccc aagaggtgaa caaggtctta ctgggatgcc tggaatccgt
     4801 ggcccaccag gaccttctgg agacccagga aagccaggtc tcacaggacc ccagggacct
     4861 cagggacttc ccggtacccc tggccgacca ggaataaaag gtgaaccagg agctccaggc
     4921 aagatcgtga cttcggaggg gtcatcgatg ctcactgtcc caggcccccc aggacctcct
     4981 ggagccatgg gacccccagg acctccaggt gccccaggcc ctgccggccc agctggtctc
     5041 ccaggacatc aagaagttct taatttacaa ggtcccccag gcccacccgg cccacgcggg
     5101 ccaccagggc cttccattcc aggcccacca ggaccccgag gcccaccagg ggagggtttg
     5161 ccaggcccac caggcccacc aggatcgttc ctgtccaact cagaaacctt cctctccggc
     5221 cccccaggcc cacctggccc cccaggtccc aagggagacc aaggtccccc aggccccaga
     5281 ggacaccaag gcgagcaagg cctcccaggt ttctcaacct cagggtccag ttctttcgga
     5341 ctcaaccttc agggaccacc aggcccacct ggcccccagg gacccaaagg tgacaaaggt
     5401 gatccaggtg ttccaggggc tcttggcatt cctagtggtc cttctgaagg gggatcatca
     5461 agtaccatgt acgtgtcagg cccgccaggg ccccctgggc cccctgggcc tccgggctct
     5521 atcagcagct ctggccagga gattcagcag tacatctctg agtacatgca gagtgacagt
     5581 attagatctt acctatccgg agttcagggt cccccaggcc cacctggtcc cccaggacct
     5641 gtcaccacca tcacaggcga gactttcgac tactcagagc tggcaagcca cgttgtgagc
     5701 tacttacgga cttcggggta cggtgtcagc ttgttctcgt cctccatctc ttctgaagac
     5761 attctggctg tgctgcagcg ggatgacgtg cgtcagtacc tacgtcagta cttgatgggc
     5821 cctcggggtc cgccagggcc accaggagcc agtggagatg ggtccctcct gtctttggac
     5881 tatgcagagc tgagtagtcg cattctcagc tacatgtcga gttctgggat cagcattggg
     5941 cttcctggtc ccccggggcc ccctggcttg ccgggaacct cctatgagga gctcctctcc
     6001 ttgctgcgag ggtctgaatt cagaggcatc gttggacccc caggtccccc gggtccacca
     6061 gggatcccag gcaatgtgtg gtccagcatc agcgtggagg acctctcgtc ttacttacat
     6121 actgccggct tgtcattcat cccaggccct ccaggacctc ctggtccccc agggcctcga
     6181 gggcccccgg gtgtctcagg agccctggca acctatgcag ctgaaaacag cgacagcttc
     6241 cggagcgagc tgatcagcta cctcacaagt cctgatgtgc gcagcttcat tgttggcccc
     6301 ccaggccctc ctgggccgca gggaccccct ggggacagcc gcctcctgtc cacggatgcc
     6361 tcccacagtc ggggtagcag ctcctcctca cacagctcat ctgtcaggcg gggcagctcc
     6421 tacagctctt ccatgagcac aggaggaggt ggtgcaggct ccctgggtgc aggcggtgcc
     6481 tttggtgaag ctgcaggaga caggggtccc tatggcactg acatcggccc aggcggaggc
     6541 tatggggcag cagcagaagg cggcatgtat gctggcaatg gcggactatt gggagctgac
     6601 tttgctggag atctggatta caatgagctg gctgtgaggg tgtcagagag catgcagcgt
     6661 cagggcctac tgcaagggat ggcctacact gtccagggcc caccaggcca gcctgggcca
     6721 caggggccac ccggcatcag caaggtcttc tctgcctaca gcaacgtgac tgcggacctc
     6781 atggacttct tccaaactta tggagccatt caaggacccc ctgggcaaaa aggagagatg
     6841 ggcactccag gacccaaagg tgacaggggc cctgctgggc caccaggtca tcctgggcca
     6901 cctggccctc gaggacacaa gggagaaaaa ggagacaaag gtgaccaagt ctatgctggg
     6961 cggagaagga gaagaagtat tgctgtcaag ccgtgagcgg ccgcgaagga tctgcgatcg
     7021 ctccggtgcc cgtcagtggg cagagcgcac atcgcccaca gtccccgaga agttgggggg
     7081 aggggtcggc aattgaacgg gtgcctagag aaggtggcgc ggggtaaact gggaaagtga
     7141 tgtcgtgtac tggctccgcc tttttcccga gggtggggga gaaccgtata taagtgcagt
     7201 agtcgccgtg aacgttcttt ttcgcaacgg gtttgccgcc agaacacagc tgaagcttcg
     7261 aggggctcgc atctctcctt cacgcgcccg ccgccctacc tgaggccgcc atccacgccg
     7321 gttgagtcgc gttctgccgc ctcccgcctg tggtgcctcc tgaactgcgt ccgccgtcta
     7381 ggtaagttta aagctcaggt cgagaccggg cctttgtccg gcgctccctt ggagcctacc
     7441 tagactcagc cggctctcca cgctttgcct gaccctgctt gctcaactct acgtctttgt
     7501 ttcgttttct gttctgcgcc gttacagatc caagctgtga ccggcgccta cgctagacgc
     7561 caccatggag agcgacgaga gcggcctgcc cgccatggag atcgagtgcc gcatcaccgg
     7621 caccctgaac ggcgtggagt tcgagctggt gggcggcgga gagggcaccc ccaagcaggg
     7681 ccgcatgacc aacaagatga agagcaccaa aggcgccctg accttcagcc cctacctgct
     7741 gagccacgtg atgggctacg gcttctacca cttcggcacc taccccagcg gctacgagaa
     7801 ccccttcctg cacgccatca acaacggcgg ctacaccaac acccgcatcg agaagtacga
     7861 ggacggcggc gtgctgcacg tgagcttcag ctaccgctac gaggccggcc gcgtgatcgg
     7921 cgacttcaag gtggtgggca ccggcttccc cgaggacagc gtgatcttca ccgacaagat
     7981 catccgcagc aacgccaccg tggagcacct gcaccccatg ggcgataacg tgctggtggg
     8041 cagcttcgcc cgcaccttca gcctgcgcga cggcggctac tacagcttcg tggtggacag
     8101 ccacatgcac ttcaagagcg ccatccaccc cagcatcctg cagaacgggg gccccatgtt
     8161 cgccttccgc cgcgtggagg agctgcacag caacaccgag ctgggcatcg tggagtacca
     8221 gcacgccttc aagaccccca tcgccttcgc cagatcccgc gctcagtcgt ccaattctgc
     8281 cgtggacggc accgccggac ccggctccac cggatctcgc gagggcagag gaagtcttct
     8341 aacatgcggt gacgtggagg agaatcccgg ccctatgacc gagtacaagc ccacggtgcg
     8401 cctcgccacc cgcgacgacg tccccagggc cgtacgcacc ctcgccgccg cgttcgccga
     8461 ctaccccgcc acgcgccaca ccgtcgatcc ggaccgccac atcgagcggg tcaccgagct
     8521 gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg tcgcggacga
     8581 cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg cggtgttcgc
     8641 cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg ctggccgcgc agcaacagat
     8701 ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg tggttcctgg ccaccgtcgg
     8761 cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc ccggagtgga
     8821 ggcggccgag cgcgccgggg tgcccgcctt cctggagacc tccgcgcccc gcaacctccc
     8881 cttctacgag cggctcggct tcaccgtcac cgccgacgtc gaggtgcccg aaggaccgcg
     8941 cacctggtgc atgacccgca agcccggtgc ctgaaatcaa cctctggatt acaaaatttg
     9001 tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc
     9061 tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta
     9121 taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt
     9181 ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca
     9241 gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc
     9301 ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt
     9361 gtcggggaag ctgacgtcct ttccatggct gctcgcctgt gttgccacct ggattctgcg
     9421 cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg
     9481 cctgctgccg gctctgcggc ctcttccgcg tctccgcctt cgccctcaga cgagtcggat
     9541 ctccctttgg ccgcctcccc gcctggtacc tttaagacca atgacttaca aggcagctgt
     9601 agatcttagc cactttttaa aagaaaaggg gggactggaa gggctaattc actcccaacg
     9661 aaaataagat ctgctttttg cttgtactgg gtctctctgg ttagaccaga tctgagcctg
     9721 ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt
     9781 gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc
     9841 cttttagtca gtgtggaaaa tctctagcag tagtagttca tgtcatctta ttattcagta
     9901 tttataactt gcaaagaaat gaatatcaga gagtgagagg aacttgttta ttgcagctta
     9961 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact
    10021 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggctctagct
    10081 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt
    10141 tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga
    10201 ggcttttttg gaggcctaga cttttgcaga gacggcccaa attcgtaatc atggtcatag
    10261 ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc
    10321 ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc
    10381 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa
    10441 cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg
    10501 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
    10561 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
    10621 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
    10681 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
    10741 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
    10801 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
    10861 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
    10921 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
    10981 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
    11041 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca
    11101 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
    11161 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
    11221 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
    11281 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
    11341 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
    11401 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
    11461 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
    11521 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat
    11581 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
    11641 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
    11701 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt
    11761 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
    11821 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
    11881 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
    11941 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
    12001 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga
    12061 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
    12121 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
    12181 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
    12241 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga
    12301 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
    12361 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc
    12421 attattatca tgacattaac ctataaaaat aggcgtatca cgaggccctt tcgtctcgcg
    12481 cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac ggtcacagct
    12541 tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc
    12601 gggtgtcggg gctggcttaa ctatgcggca tcagagcaga ttgtactgag agtgcaccat
    12661 atgcggtgtg aaataccgca cagatgcgta aggagaaaat accgcatcag gcgccattcg
    12721 ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc
    12781 cagctggcga aagggggatg tgctgcaagg cgattaagtt gggtaacgcc agggttttcc
    12841 cagtcacgac gttgtaaaac gacggccagt gccaagctg
//
