LOCUS       V037571                13870 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V037571
VERSION     V037571
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2317..7896
                     /label="COL27A1(NM_032888)"
                     /note="COL27A1(NM_032888)"
                     /gene="COL27A1"
     CDS             7903..7968
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        8012..8223
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             8236..8504
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      8533..8542
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             8563..9225
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             9295..9348
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             9349..9948
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    9949..10537
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             10611..10844
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    10916..11037
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      11077..11212
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(11245..11261)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    11269..11285
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(11293..11323)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    11338..11359
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(11647..12235)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(12406..13266)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(13267..13371)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     13845..13861
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc ctgatacgaa ctcggaattc gccaccatgg gagcgggatc ggcgcggggg
     2341 gcccgaggca cagcggcggc ggcggcggcg cgcggggggg ggtttctctt ctcctggatc
     2401 ttagtctcgt ttgcctgtca cctggcctcc acccaaggag ctcctgaaga tgtggacatc
     2461 ctccagcggc tgggcctcag ctggacgaag gccgggagcc ctgcaccccc gggagtcatt
     2521 cctttccagt cgggcttcat ctttacgcag cgggcccggc tccaggctcc cacgggcacc
     2581 gtcattcctg ccgccttggg cacagagctg gcactggtgc tgagcctctg ctcccaccgg
     2641 gtgaaccatg ccttcctctt cgctgtccgc agccagaaac gcaagctgca gctgggcctg
     2701 cagttcctcc ccggcaagac ggtcgtccac ctcgggtccc ggcgctcagt ggccttcgac
     2761 ctcgacatgc acgacgggcg ctggcaccac ctggccctcg agctccgagg ccgcacagtc
     2821 actctggtga ctgcctgcgg gcagcgccgg gtgcctgtcc tgctgccttt ccacagggac
     2881 cctgcactcg accctggggg ctccttcctc tttgggaaga tgaacccgca tgcagtccag
     2941 tttgaaggtg ctctctgcca gttcagtatc taccctgtga cgcaggtcgc tcacaattac
     3001 tgtacccacc tgaggaagca gtgtggacag gctgacacgt accagtcccc actgggacct
     3061 ctcttctccc aagactctgg cagacctttt accttccagt ccgacctcgc cctgctaggc
     3121 ctggagaact tgaccactgc cacaccagcc ctggggtcac tgccagcagg caggggaccc
     3181 agggggactg tggcacccgc cacgcccacc aagccccaaa ggactagccc cacaaaccct
     3241 caccagcata tggcggtggg aggcccagcc caaaccccgc tgctacctgc caagctgtca
     3301 gccagtaacg cacttgatcc catgctccca gcctctgttg gcggctctac cagaacgcct
     3361 cgccctgcgg ccgctcaacc atcacagaag atcacagcca ccaaaatccc caaaagcctc
     3421 cctaccaagc cttcggcccc ttctacttca attgtgccca tcaaaagccc ccatcctacc
     3481 cagaaaacag ctccatcttc atttacaaag tcagccctac ccactcagaa gcaagtgcca
     3541 cctacttccc gtccagttcc tgccagagtc tcccgtcccg cagagaagcc catccagagg
     3601 aacccgggaa tgcccaggcc cccaccgccc agcacccggc ccctacctcc taccaccagc
     3661 tcctctaaaa aacccattcc cacactagct cggactgagg ccaagataac cagccatgcc
     3721 agtaagccgg cctctgcccg caccagcacc cacaaacctc ccccatttac tgctttatcc
     3781 tcatctcctg cccctactcc tggttctacc aggagtactc ggccaccagc cacgatggta
     3841 cctccaactt cgggcaccag cactcccaga acagcacctg ccgtccccac tcctggctca
     3901 gctcccactg gaagcaagaa gcccattgga tcggaagcct caaagaaagc cggacccaag
     3961 agcagccccc ggaagcctgt ccccctcaga cctgggaagg cagccaggga tgtccccttg
     4021 agcgatctga caaccaggcc tagccccaga cagccccagc ccagtcagca gaccaccccg
     4081 gccctggtat tggccccggc gcaattcctg tcctccagcc cccggcccac gagcagtggc
     4141 tattcgatct tccacctggc aggatctacg cctttccctc tgctgatggg gcctccggga
     4201 cccaagggag actgtggctt gccgggtccc cctgggctac ctgggctacc tggaatccct
     4261 ggtgcacgtg ggcctcgggg tcctcctggg ccttatggaa atccaggtct ccccggccct
     4321 cctggagcca aaggacagaa aggggaccca gggctctcac caggaaaggc ccacgatggg
     4381 gcaaagggtg acatgggctt gcctgggctc tccgggaatc caggacctcc gggacgaaag
     4441 ggacacaagg gctatcctgg accggcaggg caccccggag aacaggggca gccaggacct
     4501 gagggcagcc caggggccaa aggttaccct ggcaggcagg ggttacctgg accggtagga
     4561 gatcccggcc ccaaaggcag caggggctac attgggctcc cagggctctt cggcctgcca
     4621 gggtctgatg gagaacgagg cctgcctggc gttcctggca agaggggcaa gatgggtatg
     4681 ccggggtttc ctggagtctt tggggaaaga ggccctcctg gactggatgg aaatcctgga
     4741 gaactgggcc tgccaggccc ccctggagtc cccggcctca ttggtgactt aggagtgttg
     4801 ggtccgattg gctacccggg acccaagggc atgaagggac tgatgggcag cgtgggggag
     4861 cccggactga aaggtgataa gggtgaacaa ggggttccag gtgtgtcagg agatcccgga
     4921 ttccaaggag acaaggggag ccaggggttg ccagggttcc ccggtgcacg ggggaagcca
     4981 gggcctctgg gcaaagtcgg agacaaagga tccattgggt ttcccgggcc ccctggaccc
     5041 gagggattcc caggagacat cggcccccct ggcgacaatg gcccagaagg catgaagggt
     5101 aagcctggag cccgaggcct gccgggaccc cgtgggcagc tggggcccga gggagatgag
     5161 ggacccatgg ggccgccagg ggcccctggc ttggagggtc agcctggcag gaaggggttt
     5221 cctgggaggc ccggcctgga tggcgtgaag ggggaaccag gggatcctgg tcggccgggg
     5281 cctgtgggag agcagggatt tatgggattc attggtctgg tcggggagcc aggaatcgtg
     5341 ggagaaaagg gtgatcgtgg catgatggga cccccaggcg tgcctggacc caaggggtcg
     5401 atgggtcatc ctggaatgcc aggtggtatg gggacccctg gagagcctgg accccagggt
     5461 cctccaggat ctcgaggccc accaggcatg aggggagcaa agggacgtcg gggcccccga
     5521 ggaccggacg gaccagctgg ggagcaaggg tccaggggcc tgaagggccc tccaggaccc
     5581 cagggcagac cgggccggcc tggacagcag ggtgtggctg gtgagcgagg ccacttgggc
     5641 tcgagaggct ttcctggcat cccgggtccc tcaggccccc caggcaccaa gggcctccca
     5701 ggagaaccgg gccctcaggg accccagggg ccaattgggc ctccaggaga gatgggaccc
     5761 aaggggccgc ctggtgcagt gggagaaccg ggccttcctg gggaagccgg gatgaagggt
     5821 gaccttggac ccctgggcac tcctggggag cagggcctca ttgggcaacg gggagagcca
     5881 ggccttgagg gtgacagtgg ccccatggga cctgatgggc tgaaggggga caggggagac
     5941 ccagggcctg atggagaaca tggcgagaaa ggccaggaag ggctgatggg tgaggacggg
     6001 ccccccggcc cccctggcgt cactggtgtc cggggtcctg aaggaaaatc agggaagcaa
     6061 ggcgagaagg gccgcactgg agccaagggt gccaagggct atcaaggaca gctgggtgag
     6121 atgggcgtcc ctggagaccc tggaccccct ggcactccag gccctaaagg gtcccggggc
     6181 agcctgggac caacgggtgc tccgggacgc atgggggccc aaggagaacc gggactggct
     6241 ggttatgatg gacacaaagg cattgtggga ccccttggac ctcctggacc aaaaggcgaa
     6301 aagggggagc agggcgagga cggcaaggct gaggggcccc ctgggccacc tggagatcgg
     6361 ggccctgtgg gtgatcgagg agaccgcggg gaaccgggag accctgggta ccctggacag
     6421 gagggtgtgc aaggcctccg tggaaagcca ggccagcagg gccaacccgg gcatccggga
     6481 ccccgggggt ggccgggacc caaaggatcg aaaggcgcag agggaccaaa gggaaagcaa
     6541 ggcaaggcag gggccccagg ccggaggggg gtccagggcc tgcaggggct gccagggccc
     6601 cggggcgtgg tggggagaca gggcctcgag ggcatcgctg gaccagatgg gcttcctggc
     6661 agggacgggc aagcaggaca gcagggggag cagggagacg atggggaccc tggccccatg
     6721 ggccctgctg ggaagagagg aaatccaggt gtggccggct tacctggagc acagggaccc
     6781 ccaggattca agggtgagag tgggttaccc ggacagctgg gtccccctgg caagcgagga
     6841 acagagggca gaacggggct ccctggaaac cagggggagc ctgggtccaa aggccagccg
     6901 ggcgactctg gcgagatggg cttcccagga atggcaggtc tcttcggacc caagggcccg
     6961 cctggagaca ttggcttcaa aggcatccag ggccctcggg ggccacctgg cttgatggga
     7021 aaggaaggca tcgtcgggcc cctcggaatc ctgggacctt cgggactccc gggtccgaag
     7081 ggtgacaaag gcagccgtgg ggactgggga ttgcaaggtc cgaggggtcc tcccggcccc
     7141 agagggcggc ccggcccccc gggtcctcca gggggtccta tccaattgca acaagatgat
     7201 cttggggcag ctttccagac gtggatggac accagtggag cactcaggcc agagagttac
     7261 agctatccag accggctggt gctggaccag ggaggagaga tctttaaaac cttacactac
     7321 ctcagcaacc tcatccagag cattaagacg cccctgggca ccaaagagaa ccccgcccgg
     7381 gtctgcaggg acctcatgga ctgtgagcag aagatggtgg atggtaccta ctgggtggat
     7441 ccaaaccttg gctgctcctc tgacaccatc gaggtctcct gcaacttcac tcatggtgga
     7501 cagacgtgtc tcaagcccat cacggcctcc aaggtcgagt ttgccatcag ccgggtccag
     7561 atgaatttcc tgcacctgct aagctccgag gtgacccagc acatcaccat ccactgcctt
     7621 aacatgaccg tgtggcagga gggcactggg cagaccccag ccaagcaggc cgtacgcttc
     7681 cgggcctgga atggacagat ttttgaagct gggggtcagt tccggcccga ggtgtccatg
     7741 gatggctgca aggtccaaga tggccgctgg catcagacac tcttcacctt ccggacccaa
     7801 gacccccaac agctgcccat catcagtgtg gacaacctcc ctcctgcctc atcagggaag
     7861 cagtaccgcc tggaagttgg acctgcgtgc ttcctcggat ccgactacaa agaccatgac
     7921 ggtgattata aagatcatga catcgactac aaggatgacg atgacaagta gcggccgcga
     7981 aggatctgcg atcgctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc
     8041 gagaagttgg ggggaggggt cggcaattga acgggtgcct agagaaggtg gcgcggggta
     8101 aactgggaaa gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg
     8161 tatataagtg cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca
     8221 cagctgaagc ttcgaggggc tcgcatctct ccttcacgcg cccgccgccc tacctgaggc
     8281 cgccatccac gccggttgag tcgcgttctg ccgcctcccg cctgtggtgc ctcctgaact
     8341 gcgtccgccg tctaggtaag tttaaagctc aggtcgagac cgggcctttg tccggcgctc
     8401 ccttggagcc tacctagact cagccggctc tccacgcttt gcctgaccct gcttgctcaa
     8461 ctctacgtct ttgtttcgtt ttctgttctg cgccgttaca gatccaagct gtgaccggcg
     8521 cctacgctag acgccaccat ggagagcgac gagagcggcc tgcccgccat ggagatcgag
     8581 tgccgcatca ccggcaccct gaacggcgtg gagttcgagc tggtgggcgg cggagagggc
     8641 acccccaagc agggccgcat gaccaacaag atgaagagca ccaaaggcgc cctgaccttc
     8701 agcccctacc tgctgagcca cgtgatgggc tacggcttct accacttcgg cacctacccc
     8761 agcggctacg agaacccctt cctgcacgcc atcaacaacg gcggctacac caacacccgc
     8821 atcgagaagt acgaggacgg cggcgtgctg cacgtgagct tcagctaccg ctacgaggcc
     8881 ggccgcgtga tcggcgactt caaggtggtg ggcaccggct tccccgagga cagcgtgatc
     8941 ttcaccgaca agatcatccg cagcaacgcc accgtggagc acctgcaccc catgggcgat
     9001 aacgtgctgg tgggcagctt cgcccgcacc ttcagcctgc gcgacggcgg ctactacagc
     9061 ttcgtggtgg acagccacat gcacttcaag agcgccatcc accccagcat cctgcagaac
     9121 gggggcccca tgttcgcctt ccgccgcgtg gaggagctgc acagcaacac cgagctgggc
     9181 atcgtggagt accagcacgc cttcaagacc cccatcgcct tcgccagatc ccgcgctcag
     9241 tcgtccaatt ctgccgtgga cggcaccgcc ggacccggct ccaccggatc tcgcgagggc
     9301 agaggaagtc ttctaacatg cggtgacgtg gaggagaatc ccggccctat gaccgagtac
     9361 aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca gggccgtacg caccctcgcc
     9421 gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg atccggaccg ccacatcgag
     9481 cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg
     9541 tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca cgccggagag cgtcgaagcg
     9601 ggggcggtgt tcgccgagat cggcccgcgc atggccgagt tgagcggttc ccggctggcc
     9661 gcgcagcaac agatggaagg cctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc
     9721 ctggccaccg tcggcgtctc gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg
     9781 ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga gacctccgcg
     9841 ccccgcaacc tccccttcta cgagcggctc ggcttcaccg tcaccgccga cgtcgaggtg
     9901 cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg gtgcctgaaa tcaacctctg
     9961 gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta
    10021 tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt
    10081 ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc
    10141 aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt
    10201 gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg
    10261 gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac
    10321 aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc ctgtgttgcc
    10381 acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac
    10441 cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct
    10501 cagacgagtc ggatctccct ttgggccgcc tccccgcctg gtacctttaa gaccaatgac
    10561 ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac tggaagggct
    10621 aattcactcc caacgaagat aagatctgct ttttgcttgt actgggtctc tctggttaga
    10681 ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata
    10741 aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta
    10801 gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtagta gttcatgtca
    10861 tcttattatt cagtatttat aacttgcaaa gaaatgaata tcagagagtg agaggaactt
    10921 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa
    10981 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca
    11041 tgtctggctc tagctatccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt
    11101 tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc
    11161 gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg cctagacttt
    11221 tgcagagacc aaattcgtaa tcatgtcata gctgtttcct gtgtgaaatt gttatccgct
    11281 cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg
    11341 agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct
    11401 gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg
    11461 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
    11521 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
    11581 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
    11641 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
    11701 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
    11761 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
    11821 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
    11881 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
    11941 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
    12001 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
    12061 gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc
    12121 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
    12181 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
    12241 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
    12301 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
    12361 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
    12421 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
    12481 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
    12541 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
    12601 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
    12661 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
    12721 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
    12781 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
    12841 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
    12901 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
    12961 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
    13021 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
    13081 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
    13141 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
    13201 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
    13261 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
    13321 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
    13381 ccgaaaagtg ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa
    13441 taggcgtatc acgaggccct ttcgtctcgc gcgtttcggt gatgacggtg aaaacctctg
    13501 acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca
    13561 agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggctggctta actatgcggc
    13621 atcagagcag attgtactga gagtgcacca tatgcggtgt gaaataccgc acagatgcgt
    13681 aaggagaaaa taccgcatca ggcgccattc gccattcagg ctgcgcaact gttgggaagg
    13741 gcgatcggtg cgggcctctt cgctattacg ccagctggcg aaagggggat gtgctgcaag
    13801 gcgattaagt tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa cgacggccag
    13861 tgccaagctg
//
