LOCUS       V028147                10522 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V028147
VERSION     V028147
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2317..4548
                     /label="COL8A1(NM_001850)"
                     /note="COL8A1(NM_001850)"
                     /gene="COL8A1"
     CDS             4555..4620
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        4664..4875
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             4888..5156
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      5185..5194
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             5215..5877
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             5947..6000
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             6001..6600
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    6601..7189
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7263..7496
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    7568..7689
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      7729..7864
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(7897..7913)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    7921..7937
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(7945..7975)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    7990..8011
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8299..8887)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9058..9918)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(9919..10023)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     10497..10513
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc ctgatacgaa ctcggaattc gccaccatgg ctgtgctgcc tggccctctg
     2341 cagctgctgg gagtgctgct taccatttcc ctgagttcca tcaggctcat tcaggctggt
     2401 gcctactatg ggatcaagcc gctgccacct caaattcctc ctcagatgcc accacaaatt
     2461 ccacaatacc agcccctggg tcagcaagta cctcacatgc ctttggccaa agatggcctt
     2521 gccatgggca aggagatgcc ccacttgcag tatggcaaag agtatccaca cctaccccaa
     2581 tatatgaagg aaattcaacc ggcgccaaga atgggcaagg aagccgtacc caagaaaggc
     2641 aaagaaatac cattagccag tttacgaggg gaacaaggtc cccgtggaga gcctggccca
     2701 agaggaccac ctgggccccc tggtttgcca ggtcatggga tacctggaat taaaggaaaa
     2761 ccagggccac agggatatcc aggagttgga aagccaggta tgcctggaat gccagggaag
     2821 ccaggagcca tgggcatgcc tggggcaaaa ggagaaattg gacagaaagg ggaaattggg
     2881 cctatgggga tcccaggacc acaaggacct ccagggcctc atggacttcc tggcattggg
     2941 aagccaggtg ggccagggtt accagggcaa ccaggaccaa agggtgatcg aggacccaaa
     3001 ggactaccag gacctcaagg ccttcggggt cctaaaggag acaagggctt cgggatgcca
     3061 ggtgcgccag gtgtaaaggg gcctccaggg atgcacggcc ctcccggccc tgttggactg
     3121 ccaggagtgg gcaaaccagg agtgacaggc ttccctgggc cccagggccc cctgggaaag
     3181 ccaggggctc caggagaacc tgggccacaa ggccctattg gggtaccggg ggttcaagga
     3241 cctcctggga tacccggaat tggaaagcca ggccaggatg ggatcccagg ccagccagga
     3301 tttccaggtg gcaaagggga gcaaggactg ccagggctac caggaccccc aggccttcca
     3361 gggattggga aaccaggctt cccaggaccc aaaggtgacc ggggcatggg aggtgttcct
     3421 ggggctcttg gaccaagagg ggagaaagga ccaataggtg ccccaggaat agggggtcct
     3481 ccaggagagc caggcctgcc tggaatccca ggtcctatgg gccctccagg tgctattggt
     3541 tttcctggac ccaaaggaga aggtgggatt gtagggccac aggggccacc aggtcccaag
     3601 ggtgagccag ggcttcaagg cttcccagga aagccaggtt tccttggtga agtagggcct
     3661 cctggcatga ggggtttgcc aggtcccata gggcccaagg gggaagctgg gcaaaaaggt
     3721 gtaccaggac tccctggtgt tccagggctt ctcggaccta agggagagcc aggaatccca
     3781 ggggatcagg gtttacaggg ccccccaggt atcccaggga ttgggggccc tagtggcccc
     3841 attggaccac ctgggattcc aggccccaaa ggggagccgg gcctcccagg gccccctggg
     3901 ttccctggta tagggaaacc cggagtggca ggacttcatg gccccccagg gaagcctggt
     3961 gcccttggtc ctcaaggcca gcctggcctt ccaggacccc caggccctcc aggacctcca
     4021 ggacccccag ctgtgatgcc ccctacacca ccaccccagg gagagtatct gccagatatg
     4081 gggctgggaa ttgatggcgt gaaacccccc catgcctacg gggctaagaa aggcaagaat
     4141 ggagggccag cctatgagat gcctgcattt accgccgagc taaccgcacc tttcccaccg
     4201 gtgggggccc cagtgaagtt taacaaactg ctgtataacg gcagacagaa ctacaacccg
     4261 cagacaggca tcttcacctg tgaggtccct ggtgtctact actttgcata ccacgttcac
     4321 tgcaaggggg gcaacgtgtg ggttgctcta ttcaagaaca acgagcccgt gatgtacacg
     4381 tacgacgagt acaaaaaggg cttcctggac caggcatctg ggagtgcagt gctgctgctc
     4441 aggcccggag accgggtgtt cctccagatg ccctcagaac aggctgcagg actgtatgcc
     4501 gggcagtatg tccactcctc cttttcagga tatttattgt atcccatggg atccgactac
     4561 aaagaccatg acggtgatta taaagatcat gacatcgact acaaggatga cgatgacaag
     4621 tagcggccgc gaaggatctg cgatcgctcc ggtgcccgtc agtgggcaga gcgcacatcg
     4681 cccacagtcc ccgagaagtt ggggggaggg gtcggcaatt gaacgggtgc ctagagaagg
     4741 tggcgcgggg taaactggga aagtgatgtc gtgtactggc tccgcctttt tcccgagggt
     4801 gggggagaac cgtatataag tgcagtagtc gccgtgaacg ttctttttcg caacgggttt
     4861 gccgccagaa cacagctgaa gcttcgaggg gctcgcatct ctccttcacg cgcccgccgc
     4921 cctacctgag gccgccatcc acgccggttg agtcgcgttc tgccgcctcc cgcctgtggt
     4981 gcctcctgaa ctgcgtccgc cgtctaggta agtttaaagc tcaggtcgag accgggcctt
     5041 tgtccggcgc tcccttggag cctacctaga ctcagccggc tctccacgct ttgcctgacc
     5101 ctgcttgctc aactctacgt ctttgtttcg ttttctgttc tgcgccgtta cagatccaag
     5161 ctgtgaccgg cgcctacgct agacgccacc atggagagcg acgagagcgg cctgcccgcc
     5221 atggagatcg agtgccgcat caccggcacc ctgaacggcg tggagttcga gctggtgggc
     5281 ggcggagagg gcacccccaa gcagggccgc atgaccaaca agatgaagag caccaaaggc
     5341 gccctgacct tcagccccta cctgctgagc cacgtgatgg gctacggctt ctaccacttc
     5401 ggcacctacc ccagcggcta cgagaacccc ttcctgcacg ccatcaacaa cggcggctac
     5461 accaacaccc gcatcgagaa gtacgaggac ggcggcgtgc tgcacgtgag cttcagctac
     5521 cgctacgagg ccggccgcgt gatcggcgac ttcaaggtgg tgggcaccgg cttccccgag
     5581 gacagcgtga tcttcaccga caagatcatc cgcagcaacg ccaccgtgga gcacctgcac
     5641 cccatgggcg ataacgtgct ggtgggcagc ttcgcccgca ccttcagcct gcgcgacggc
     5701 ggctactaca gcttcgtggt ggacagccac atgcacttca agagcgccat ccaccccagc
     5761 atcctgcaga acgggggccc catgttcgcc ttccgccgcg tggaggagct gcacagcaac
     5821 accgagctgg gcatcgtgga gtaccagcac gccttcaaga cccccatcgc cttcgccaga
     5881 tcccgcgctc agtcgtccaa ttctgccgtg gacggcaccg ccggacccgg ctccaccgga
     5941 tctcgcgagg gcagaggaag tcttctaaca tgcggtgacg tggaggagaa tcccggccct
     6001 atgaccgagt acaagcccac ggtgcgcctc gccacccgcg acgacgtccc cagggccgta
     6061 cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc gccacaccgt cgatccggac
     6121 cgccacatcg agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac
     6181 atcggcaagg tgtgggtcgc ggacgacggc gccgcggtgg cggtctggac cacgccggag
     6241 agcgtcgaag cgggggcggt gttcgccgag atcggcccgc gcatggccga gttgagcggt
     6301 tcccggctgg ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg gcccaaggag
     6361 cccgcgtggt tcctggccac cgtcggcgtc tcgcccgacc accagggcaa gggtctgggc
     6421 agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg
     6481 gagacctccg cgccccgcaa cctccccttc tacgagcggc tcggcttcac cgtcaccgcc
     6541 gacgtcgagg tgcccgaagg accgcgcacc tggtgcatga cccgcaagcc cggtgcctga
     6601 aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct
     6661 ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt
     6721 atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg
     6781 tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact
     6841 ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct
     6901 attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg
     6961 ttgggcactg acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc
     7021 gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc
     7081 aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt
     7141 cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgcc tggtaccttt
     7201 aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag aaaagggggg
     7261 actggaaggg ctaattcact cccaacgaag ataagatctg ctttttgctt gtactgggtc
     7321 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct
     7381 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga
     7441 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtag
     7501 tagttcatgt catcttatta ttcagtattt ataacttgca aagaaatgaa tatcagagag
     7561 tgagaggaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
     7621 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
     7681 tgtatcttat catgtctggc tctagctatc ccgcccctaa ctccgcccat cccgccccta
     7741 actccgccca gttccgccca ttctccgccc catggctgac taattttttt tatttatgca
     7801 gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga
     7861 ggcctagact tttgcagaga ccaaattcgt aatcatgtca tagctgtttc ctgtgtgaaa
     7921 ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg
     7981 gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca
     8041 gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg
     8101 tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
     8161 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
     8221 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
     8281 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
     8341 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     8401 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
     8461 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
     8521 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
     8581 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
     8641 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
     8701 gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc
     8761 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
     8821 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
     8881 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
     8941 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
     9001 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
     9061 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
     9121 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
     9181 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca
     9241 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
     9301 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
     9361 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
     9421 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
     9481 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
     9541 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
     9601 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
     9661 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat
     9721 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
     9781 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
     9841 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
     9901 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
     9961 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
    10021 gcgcacattt ccccgaaaag tgccacctga cgtctaagaa accattatta tcatgacatt
    10081 aacctataaa aataggcgta tcacgaggcc ctttcgtctc gcgcgtttcg gtgatgacgg
    10141 tgaaaacctc tgacacatgc agctcccgga gacggtcaca gcttgtctgt aagcggatgc
    10201 cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc ggggctggct
    10261 taactatgcg gcatcagagc agattgtact gagagtgcac catatgcggt gtgaaatacc
    10321 gcacagatgc gtaaggagaa aataccgcat caggcgccat tcgccattca ggctgcgcaa
    10381 ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta cgccagctgg cgaaaggggg
    10441 atgtgctgca aggcgattaa gttgggtaac gccagggttt tcccagtcac gacgttgtaa
    10501 aacgacggcc agtgccaagc tg
//
