LOCUS       V047307                11146 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V047307
VERSION     V047307
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1920
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2010..2213
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2302..6000
                     /label="COPA(NM_001098398)"
                     /note="COPA(NM_001098398)"
                     /gene="COPA"
     CDS             6007..6072
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        6116..6327
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             6340..6608
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     CDS             6635..7234
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    7241..7829
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7903..8136
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    8208..8329
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      8348..8483
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(8521..8537)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8545..8561
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8569..8599)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8614..8635
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8923..9511)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9682..10542)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10543..10647)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     11121..11137
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt
     1921 atcgatacta gtattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
     1981 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
     2041 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
     2101 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
     2161 tgacgcaaat gggcggtagg cgtgtacggt gggaggttta tataagcaga gctcgtttag
     2221 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagattct
     2281 agagctagcg aattcgccac catgttaacc aaattcgaga ccaagagcgc gcgggtcaaa
     2341 gggctcagct ttcaccccaa aagaccttgg atcctgacta gtttacataa tggggtcatc
     2401 cagttatggg actatcggat gtgcactctc attgacaagt ttgatgaaca tgatggtcca
     2461 gtgcgaggca ttgacttcca taagcagcag ccactgttcg tctctggagg agatgactat
     2521 aagattaagg tttggaatta caagcttcgg cgctgtcttt tcacattgct tgggcactta
     2581 gattatattc gcaccacgtt ttttcatcat gaatatccct ggattctgag tgcctccgat
     2641 gatcagacca tccgagtgtg gaactggcaa tctagaacct gtgtttgtgt gttaacaggg
     2701 cacaaccatt atgtgatgtg tgctcagttc caccccacag aagacttggt agtatcagcc
     2761 agcctggacc agactgtgcg cgtttgggat atttctggtc tgaggaaaaa aaacctgtcc
     2821 cctggtgcgg tggaatcgga tgtgagagga ataactgggg ttgatctatt tggaactaca
     2881 gatgcagtgg tgaagcatgt actagagggt cacgatcgtg gagtaaactg ggctgccttc
     2941 caccccacta tgccccttat tgtatctggg gcagatgatc gtcaagtgaa gatctggcgc
     3001 atgaatgaat caaaggcatg ggaggttgat acctgccggg gccattacaa caatgtatct
     3061 tgtgccgtct tccaccctcg ccaagagttg atcctcagca attctgagga caagagtatt
     3121 cgagtctggg atatgtctaa gcggactggg gttcagactt tccgcagaga ccatgatcgt
     3181 ttctgggtcc tagctgctca ccctaacctt aacctctttg cagcaggcca tgatggtggt
     3241 atgattgtgt ttaagctgga acgggaacgg ccagcctatg ctgttcatgg caatatgcta
     3301 cactatgtca aggaccgatt cttacgacag ctggatttca acagctccaa agatgtagct
     3361 gtgatgcagt tgcggagtgg ttccaagttt ccagtattca atatgtcata caatccagca
     3421 gaaaatgcag tcctgctttg tacaagagct agcaatctag agaatagtac ctatgacctg
     3481 tacaccatcc ctaaagatgc tgactcccag aatcctgatg cgcctgaagg gaaacgatcc
     3541 tcaggcctga cagccgtttg ggtcgctcga aatcggtttg ctgtcctaga tcggatgcat
     3601 tcgcttctga tcaagaatct gaagaatgag atcaccaaaa aggtacaggt gcccaactgt
     3661 gatgagatct tctatgctgg cacaggcaat ctcctgcttc gagatgcgga ctctatcaca
     3721 ctctttgacg tacagcagaa gcggactctg gcatctgtga agatttctaa agtgaaatac
     3781 gttatctggt cagcagacat gtcacatgta gcactactag ccaaacacga acactcatgc
     3841 cctttgcctc ttacagccat tgtgatctgt aaccgcaaac tggatgcttt atgtaacatt
     3901 catgagaaca ttcgtgtcaa gagtggggcc tgggatgaga gtggggtatt tatctatacc
     3961 acaagcaacc acatcaaata tgctgtcacc actggggacc acgggatcat tcgaactctg
     4021 gatttaccca tctatgtcac acgggtgaag ggcaacaatg tatactgcct agacagggag
     4081 tgtcgtcccc gggtactcac cattgatccc actgagttca aattcaagct ggccctgatc
     4141 aacagaaaat atgatgaggt actgcacatg gtgaggaatg ccaaactagt tggccagtct
     4201 attattgctt atctccagaa gaagggctat cctgaagtgg cactgcattt tgtcaaggat
     4261 gagaaaactc gctttagtct ggcactggag tgtggaaaca ttgagattgc tctggaagca
     4321 gccaaagcac tggatgacaa gaactgctgg gaaaagctgg gagaagtggc cctgctgcag
     4381 gggaaccacc agattgtgga aatgtgctat cagcgtacca aaaactttga caaactttcc
     4441 ttcctgtatc ttatcactgg caacttagaa aaacttcgca agatgatgaa gattgctgag
     4501 atcagaaagg acatgagtgg ccactatcag aatgccctat acctgggtga tgtgtcagag
     4561 cgtgtgcgga tcctgaagaa ctgtggacag aagtccctgg cctatctcac agctgctacc
     4621 catggcttag atgaagaagc tgagagccta aaggagacat ttgacccaga gaaggagaca
     4681 atcccagaca ttgaccctaa tgccaagctg ctccagccac ctgcacctat catgccattg
     4741 gataccaatt ggcctttatt gactgtatcc aaaggatttt ttgaaggcac cattgccagc
     4801 aaagggaagg gaggagcact ggctgctgac attgacattg acactgttgg tacagagggc
     4861 tggggagagg atgcagagct gcagttggat gaagatgggt ttgtggaggc tacagaaggt
     4921 ttgggggatg atgctcttgg caagggacag gaagaaggag gtggctggga tgtagaagaa
     4981 gatctggagc tccctcctga gctggatata tcccctgggg cagctggtgg ggctgaagat
     5041 ggtttctttg tgcccccaac caagggaaca agtccaactc agatctggtg taataactct
     5101 cagcttccag ttgatcacat cctggcaggc tctttcgaaa cagccatgcg gctccttcat
     5161 gaccaagtag gggtaatcca gtttggcccc tacaagcaac tgttcctaca gacatacgcc
     5221 cgaggccgca caacctatca ggctctgccc tgcctaccct ccatgtatgg ctatcctaat
     5281 cgcaactgga aggatgcagg gctgaagaat ggtgtaccag ctgtgggcct gaagcttaat
     5341 gacctcatcc aacggttgca gctgtgctac cagctcacca cagttggcaa atttgaggag
     5401 gctgtggaaa aattccgttc catccttctc agtgtgccac ttcttgttgt ggacaataaa
     5461 caagagattg cagaggccca gcagctcatc accatttgcc gtgagtacat tgtgggtttg
     5521 tccgtggaga cagaaaggaa gaagctgccc aaagagactc tagaacagca gaagcgcatc
     5581 tgtgagatgg cagcctattt cacccactca aacctgcagc ctgtgcacat gatcctggtg
     5641 ctgcgtacag ccctcaatct gttcttcaag ctcaagaact tcaagacagc tgccaccttt
     5701 gctcggcgcc tactagaact cgggcccaag cctgaggtgg cccaacagac ccgaaaaatc
     5761 ctgtctgcct gtgagaagaa tcccacagat gcctaccagc tcaattatga catgcacaac
     5821 ccctttgaca tttgtgctgc atcatatcgg cccatctacc gtggaaagcc agtagaaaag
     5881 tgtccactca gtggggcctg ctattcccct gagttcaaag gtcaaatctg cagggtcacc
     5941 acagtgacag agattggcaa agatgtgatt ggtttaagga tcagtcctct gcagtttcgc
     6001 ggatccgact acaaagacca tgacggtgat tataaagatc atgacatcga ctacaaggat
     6061 gacgatgaca agtagcggcc gcgaaggatc tgcgatcgct ccggtgcccg tcagtgggca
     6121 gagcgcacat cgcccacagt ccccgagaag ttggggggag gggtcggcaa ttgaacgggt
     6181 gcctagagaa ggtggcgcgg ggtaaactgg gaaagtgatg tcgtgtactg gctccgcctt
     6241 tttcccgagg gtgggggaga accgtatata agtgcagtag tcgccgtgaa cgttcttttt
     6301 cgcaacgggt ttgccgccag aacacagctg aagcttcgag gggctcgcat ctctccttca
     6361 cgcgcccgcc gccctacctg aggccgccat ccacgccggt tgagtcgcgt tctgccgcct
     6421 cccgcctgtg gtgcctcctg aactgcgtcc gccgtctagg taagtttaaa gctcaggtcg
     6481 agaccgggcc tttgtccggc gctcccttgg agcctaccta gactcagccg gctctccacg
     6541 ctttgcctga ccctgcttgc tcaactctac gtctttgttt cgttttctgt tctgcgccgt
     6601 tacagatcca agctgtgacc ggcgcctacg ctagatgacc gagtacaagc ccacggtgcg
     6661 cctcgccacc cgcgacgacg tcccccgggc cgtacgcacc ctcgccgccg cgttcgccga
     6721 ctaccccgcc acgcgccaca ccgtcgaccc ggaccgccac atcgagcggg tcaccgagct
     6781 gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg tcgcggacga
     6841 cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg cggtgttcgc
     6901 cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg ctggccgcgc agcaacagat
     6961 ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg tggttcctgg ccaccgtcgg
     7021 cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc ccggagtgga
     7081 ggcggccgag cgcgccgggg tgcccgcctt cctggagacc tccgcgcccc gcaacctccc
     7141 cttctacgag cggctcggct tcaccgtcac cgccgacgtc gaggtgcccg aaggaccgcg
     7201 cacctggtgc atgacccgca agcccggtgc ctgagtcgac aatcaacctc tggattacaa
     7261 aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata
     7321 cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc
     7381 cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg
     7441 tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac
     7501 ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat
     7561 cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt
     7621 ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg ccacctggat
     7681 tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc
     7741 ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag
     7801 tcggatctcc ctttgggccg cctccccgcc tggtaccttt aagaccaatg acttacaagg
     7861 cagctgtaga tcttagccac tttttaaaag aaaagggggg actggaaggg ctaattcact
     7921 cccaacgaaa ataagatctg ctttttgctt gtactgggtc tctctggtta gaccagatct
     7981 gagcctggga gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc
     8041 cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc
     8101 tcagaccctt ttagtcagtg tggaaaatct ctagcagtag tagttcatgt catcttatta
     8161 ttcagtattt ataacttgca aagaaatgaa tatcagagag tgagaggaac ttgtttattg
     8221 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt
     8281 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctggc
     8341 tctagctatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact
     8401 aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta
     8461 gtgaggaggc ttttttggag gcctagactt ttgcagagac ggcccaaatt cgtaatcatg
     8521 gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc
     8581 cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc
     8641 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat
     8701 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac
     8761 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
     8821 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
     8881 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
     8941 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     9001 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     9061 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
     9121 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     9181 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     9241 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
     9301 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
     9361 aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
     9421 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
     9481 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
     9541 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
     9601 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
     9661 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
     9721 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
     9781 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
     9841 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
     9901 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
     9961 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc
    10021 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
    10081 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
    10141 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
    10201 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
    10261 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca
    10321 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
    10381 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
    10441 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
    10501 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
    10561 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
    10621 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta
    10681 agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga ggccctttcg
    10741 tctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac atgcagctcc cggagacggt
    10801 cacagcttgt ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg cgtcagcggg
    10861 tgttggcggg tgtcggggct ggcttaacta tgcggcatca gagcagattg tactgagagt
    10921 gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc gcatcaggcg
    10981 ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct
    11041 attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg
    11101 gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc aagctg
//
