LOCUS       V032596                10569 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V032596
VERSION     V032596
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1798..1915
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2005..2208
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2297..4588
                     /label="CUL2(NM_001198778)"
                     /note="CUL2(NM_001198778)"
                     /gene="CUL2"
     CDS             4595..4684
                     /codon_start=1
                     /product="3 tandem Myc epitope tags"
                     /transl_table=1
                     /translation="EQKLISEEDLEQKLISEEDLEQKLISEEDL"
                     /label="3xMyc"
     promoter        4728..4939
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             4952..5220
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      5249..5258
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             5279..5941
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             6011..6064
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             6065..6664
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    6665..7252
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7326..7559
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    7631..7752
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      7771..7906
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(7944..7960)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    7968..7984
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(7992..8022)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8037..8058
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8346..8934)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9105..9965)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(9966..10070)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     10544..10560
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggttaacttt
     1801 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc
     1861 aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa attttatcga
     1921 tactagtatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac
     1981 gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga
     2041 tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg
     2101 ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg
     2161 caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctcg tttagtgaac
     2221 cgtcagatcg cctggagacg ccatccacgc tgttttgacc tccatagaag attctagagc
     2281 tagcgaattc gccaccatgt acagagtaac atggtcaact ttttggctta gatttcaaca
     2341 ctacacttgc acaatgtctt tgaaaccaag agtagtagat tttgatgaaa catggaacaa
     2401 acttttgacg acaataaaag ccgtggtcat gttggaatac gtcgaaagag caacatggaa
     2461 tgaccgtttc tcagatatct atgctttatg tgtggcctat cctgaacccc ttggagaaag
     2521 actttataca gaaactaaga tttttttgga aaatcatgtt cggcatttgc ataagagagt
     2581 tttggagtca gaagaacaag tacttgttat gtatcatagg tactgggaag aatacagcaa
     2641 gggtgcagac tatatggact gcttatatag gtatctcaac acccagttta ttaaaaagaa
     2701 taaattaaca gaagcggacc ttcagtatgg ctatggtggt gtagatatga atgaaccact
     2761 tatggaaata ggagagctag cattggatat gtggaggaaa ttgatggttg aaccacttca
     2821 ggccatcctt atccgaatgc tgctccgaga aatcaaaaat gatcgtggtg gagaagaccc
     2881 aaaccagaaa gtaatccatg gggttattaa ctcctttgtt catgttgaac agtataagaa
     2941 aaaattcccc ttaaagtttt atcaggaaat ttttgagtct ccctttctga ctgaaacagg
     3001 agagtattac aaacaagaag cttcaaattt attacaagaa tcaaactgct cacagtatat
     3061 ggaaaaggtt ctaggtagat taaaagatga agaaattcga tgtcgaaaat acctacatcc
     3121 aagttcatat actaaggtga ttcatgaatg tcaacaacga atggtagcag accacttaca
     3181 gtttttacat gcagaatgtc ataatataat tcgacaagag aaaaaaaatg acatggcaaa
     3241 tatgtacgtc ttactccgtg ctgtgtccac tggtttacct catatgattc aggagctgca
     3301 aaaccacatc catgatgagg gccttcgagc aaccagcaac cttactcagg aaaacatgcc
     3361 aacactattt gtggagtcag ttttggaagt gcatggtaaa tttgttcagc ttatcaacac
     3421 tgttttgaat ggtgatcagc attttatgag tgcgttggat aaggccctta cgtcagttgt
     3481 aaattacaga gaacctaagt ctgtttgcaa agcacctgaa ctgcttgcta agtactgtga
     3541 caacttactg aagaagtcag cgaaagggat gacagagaat gaagtggaag acaggctcac
     3601 gagcttcatc acagtgttca aatacattga tgacaaggac gtctttcaaa agttctacgc
     3661 aagaatgctg gcaaaacgtt taattcatgg gttatccatg tctatggact ctgaagaagc
     3721 catgatcaac aaattaaagc aagcctgtgg ttatgagttt accagcaagc tacatcggat
     3781 gtatacagat atgagtgtca gcgctgatct caacaataag ttcaacaatt ttatcaaaaa
     3841 ccaagacaca gtaatagatt tgggaattag ttttcaaata tatgttctac aggctggtgc
     3901 gtggcctctt actcaggctc cttcatctac gtttgcaatt ccccaggaat tagaaaaaag
     3961 tgtacagatg tttgaattat tttatagcca acatttcagt ggaaggaaac ttacatggtt
     4021 acattatctg tgtacaggtg aagttaaaat gaactatttg ggcaaaccat atgtagccat
     4081 ggttacaaca taccaaatgg cagttcttct tgcctttaac aacagtgaaa ctgtcagtta
     4141 taaagagctt caggacagca ctcagatgaa tgaaaaggaa ctgacaaaaa caatcaaatc
     4201 attacttgat gtgaaaatga ttaaccatga ttcagaaaag gaagatattg atgcagaatc
     4261 ttcgttttca ttaaatatga actttagcag taaaagaaca aaatttaaaa ttactacatc
     4321 aatgcagaaa gacacaccac aagaaatgga gcagactaga agtgcagttg atgaggaccg
     4381 gaaaatgtat ctccaagctg ctatagttcg tatcatgaaa gcacgaaaag tgcttcggca
     4441 caatgccctt attcaagagg tgattagcca gtcaagagct aggtttaatc ccagtatcag
     4501 catgattaag aagtgtattg aagttctgat agacaaacaa tacatagaac gcagccaggc
     4561 gtcggcagat gaatacagct acgtcgcggg atccgaacaa aaactaatat cagaggaaga
     4621 tttggaacag aaactaatca gtgaagaaga tttagagcaa aagttaattt ccgaggagga
     4681 cttatagcgg ccgcgaagga tctgcgatcg ctccggtgcc cgtcagtggg cagagcgcac
     4741 atcgcccaca gtccccgaga agttgggggg aggggtcggc aattgaacgg gtgcctagag
     4801 aaggtggcgc ggggtaaact gggaaagtga tgtcgtgtac tggctccgcc tttttcccga
     4861 gggtggggga gaaccgtata taagtgcagt agtcgccgtg aacgttcttt ttcgcaacgg
     4921 gtttgccgcc agaacacagc tgaagcttcg aggggctcgc atctctcctt cacgcgcccg
     4981 ccgccctacc tgaggccgcc atccacgccg gttgagtcgc gttctgccgc ctcccgcctg
     5041 tggtgcctcc tgaactgcgt ccgccgtcta ggtaagttta aagctcaggt cgagaccggg
     5101 cctttgtccg gcgctccctt ggagcctacc tagactcagc cggctctcca cgctttgcct
     5161 gaccctgctt gctcaactct acgtctttgt ttcgttttct gttctgcgcc gttacagatc
     5221 caagctgtga ccggcgccta cgctagacgc caccatggag agcgacgaga gcggcctgcc
     5281 cgccatggag atcgagtgcc gcatcaccgg caccctgaac ggcgtggagt tcgagctggt
     5341 gggcggcgga gagggcaccc ccaagcaggg ccgcatgacc aacaagatga agagcaccaa
     5401 aggcgccctg accttcagcc cctacctgct gagccacgtg atgggctacg gcttctacca
     5461 cttcggcacc taccccagcg gctacgagaa ccccttcctg cacgccatca acaacggcgg
     5521 ctacaccaac acccgcatcg agaagtacga ggacggcggc gtgctgcacg tgagcttcag
     5581 ctaccgctac gaggccggcc gcgtgatcgg cgacttcaag gtggtgggca ccggcttccc
     5641 cgaggacagc gtgatcttca ccgacaagat catccgcagc aacgccaccg tggagcacct
     5701 gcaccccatg ggcgataacg tgctggtggg cagcttcgcc cgcaccttca gcctgcgcga
     5761 cggcggctac tacagcttcg tggtggacag ccacatgcac ttcaagagcg ccatccaccc
     5821 cagcatcctg cagaacgggg gccccatgtt cgccttccgc cgcgtggagg agctgcacag
     5881 caacaccgag ctgggcatcg tggagtacca gcacgccttc aagaccccca tcgccttcgc
     5941 cagatcccgc gctcagtcgt ccaattctgc cgtggacggc accgccggac ccggctccac
     6001 cggatctcgc gagggcagag gaagtcttct aacatgcggt gacgtggagg agaatcccgg
     6061 ccctatgacc gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg tccccagggc
     6121 cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca ccgtcgatcc
     6181 ggaccgccac atcgagcggg tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct
     6241 cgacatcggc aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc
     6301 ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag
     6361 cggttcccgg ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc accggcccaa
     6421 ggagcccgcg tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct
     6481 gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt
     6541 cctggagacc tccgcgcccc gcaacctccc cttctacgag cggctcggct tcaccgtcac
     6601 cgccgacgtc gaggtgcccg aaggaccgcg cacctggtgc atgacccgca agcccggtgc
     6661 ctgaaatcaa cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt
     6721 tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc
     6781 ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga
     6841 gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc
     6901 cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct
     6961 ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg
     7021 gctgttgggc actgacaatt ccgtggtgtt gtcggggaag ctgacgtcct ttccatggct
     7081 gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc
     7141 cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg
     7201 tctccgcctt cgccctcaga cgagtcggat ctccctttgg ccgcctcccc gcctggtacc
     7261 tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaaggg
     7321 gggactggaa gggctaattc actcccaacg aaaataagat ctgctttttg cttgtactgg
     7381 gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact
     7441 gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg
     7501 tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag
     7561 tagtagttca tgtcatctta ttattcagta tttataactt gcaaagaaat gaatatcaga
     7621 gagtgagagg aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac
     7681 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat
     7741 caatgtatct tatcatgtct ggctctagct atcccgcccc taactccgcc cagttccgcc
     7801 cattctccgc cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg
     7861 gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctaga cttttgcaga
     7921 gacggcccaa attcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc
     7981 acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga
     8041 gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg
     8101 tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg
     8161 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
     8221 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
     8281 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
     8341 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
     8401 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
     8461 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
     8521 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
     8581 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
     8641 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
     8701 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
     8761 tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca
     8821 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
     8881 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
     8941 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
     9001 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
     9061 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc
     9121 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc
     9181 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata
     9241 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg
     9301 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc
     9361 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct
     9421 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
     9481 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt
     9541 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca
     9601 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac
     9661 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca
     9721 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt
     9781 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
     9841 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca
     9901 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
     9961 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
    10021 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
    10081 cgaaaagtgc cacctgacgt ctaagaaacc attattatca tgacattaac ctataaaaat
    10141 aggcgtatca cgaggccctt tcgtctcgcg cgtttcggtg atgacggtga aaacctctga
    10201 cacatgcagc tcccggagac ggtcacagct tgtctgtaag cggatgccgg gagcagacaa
    10261 gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg gctggcttaa ctatgcggca
    10321 tcagagcaga ttgtactgag agtgcaccat atgcggtgtg aaataccgca cagatgcgta
    10381 aggagaaaat accgcatcag gcgccattcg ccattcaggc tgcgcaactg ttgggaaggg
    10441 cgatcggtgc gggcctcttc gctattacgc cagctggcga aagggggatg tgctgcaagg
    10501 cgattaagtt gggtaacgcc agggttttcc cagtcacgac gttgtaaaac gacggccagt
    10561 gccaagctg
//
