LOCUS       V042297                12637 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V042297
VERSION     V042297
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1920
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2010..2213
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2302..7491
                     /label="CUL7(NM_001168370)"
                     /note="CUL7(NM_001168370)"
                     /gene="CUL7"
     CDS             7498..7563
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        7607..7818
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             7831..8099
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     CDS             8126..8725
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    8732..9320
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             9394..9627
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    9699..9820
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      9839..9974
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(10012..10028)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    10036..10052
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(10060..10090)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    10105..10126
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(10414..11002)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(11173..12033)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(12034..12138)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     12612..12628
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt
     1921 atcgatacta gtattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
     1981 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
     2041 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
     2101 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
     2161 tgacgcaaat gggcggtagg cgtgtacggt gggaggttta tataagcaga gctcgtttag
     2221 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagattct
     2281 agagctagcg aattcgccac catggtggga gaactccgct acagggaatt cagggtgccc
     2341 ctggggcccg gcttacatgc ctatcctgat gagctgatcc gccagcgcgt gggccatgat
     2401 gggcatcctg agtaccagat ccgttggctc atcctgcggc gtggcgatga gggggacggg
     2461 ggctctggcc aagtggactg caaggctgag cacatcctgc tgtggatgtc caaggatgag
     2521 atctatgcca actgccacaa gatgctgggc gaggatggcc aggtcatcgg gccctcccag
     2581 gagtctgcag gggaggttgg ggccctggac aaatctgtgc tggaggagat ggaaaccgac
     2641 gtgaagtccc tcattcagag agcccttcgg cagctggagg agtgtgtggg cactatccct
     2701 cctgctcctc tacttcacac tgtccacgtg ctcagcgcct atgccagcat tgagcccctc
     2761 actggagtat tcaaggaccc aagggtcctg gacttgctca tgcacatgtt gagtagtcct
     2821 gattatcaga ttcgctggag tgcaggccgg atgatacaag ccctgtcctc ccatgacgct
     2881 ggtgaggggc agtgtgggga ggaagggaaa gcaggagaag ggctgggtcg gctcagggac
     2941 tcacaggaca ctgtggcagg agcctctgat ctcatcagga cccggactca gatccttctg
     3001 tcactgagcc aacaagaagc cattgagaaa cacctggatt ttgacagccg ctgtgctctg
     3061 ctagcactgt ttgcacaggc cacgctctct gaacacccca tgtctttcga gggcattcag
     3121 ctaccacagg tcccaggaag ggtgctcttc tccctggtga agcggtattt gcatgtcacc
     3181 tcgctcctgg atcagctgaa cgacagtgct gcggagccag gagcccagaa cacctctgct
     3241 cctgaggagt tgagtgggga gaggggtcaa ctggagctgg agttcagtat ggccatgggc
     3301 accctgatct cggagctggt gcaagccatg cgctgggacc aggcctcaga cagaccaagg
     3361 agctcagcac ggtcccccgg ttccatcttc cagcctcagc tggcagatgt gagcccaggg
     3421 ctccccgctg cccaggctca gccctccttc aggaggtcaa gacgttttcg ccctcgttct
     3481 gagttcgcaa gtggcaatac ctatgctttg tatgtgcggg acacactgca gccggggatg
     3541 cgagtgcgga tgctggatga ttatgaggag atcagtgccg gggatgaggg cgagtttcgg
     3601 cagagcaaca acggtgtgcc tcctgtgcag gtattttggg agtcaacagg ccgcacctat
     3661 tgggtgcact ggcacatgct ggagatcttg ggctttgagg aagacattga ggacatggtt
     3721 gaggctgatg agtaccaagg ggcagtggcc agtagagtcc tgggtagagc cctgcctgcc
     3781 tggcgctgga ggcccatgac agaactctat gctgtgcctt atgtgctgcc tgaggatgag
     3841 gacactgagg agtgtgaaca cctgaccctg gctgagtggt gggaactcct cttcttcatc
     3901 aagaagctgg atggacctga ccatcaggag gttctccaga tcctccagga gaacctagat
     3961 ggggagattc tggatgatga gatcctagct gaactggccg tgcccataga attggcccag
     4021 gacttgctgc tgactctgcc acagcgactc aatgacagtg ccctcaggga cctgatcaac
     4081 tgccatgtct acaagaagta tgggcctgaa gccctagcag ggaaccaagc ctacccatcc
     4141 cttctagaag cccaagaaga tgtcctcctg ctagacgcgc aggcccaggc taaggactca
     4201 gaagatgcag ccaaagtgga agcaaaagaa cccccatctc agagtcccaa cactcccctg
     4261 cagcgtctgg tggagggtta tggtccagct gggaaaatcc tcctggatct agagcaagcc
     4321 ctcagctcag aggggaccca ggagaacaag gtcaagccac tcctgctgca gctgcagcgg
     4381 cagccgcagc ccttcctggc actgatgcag agcctggaca ctccggagac taacaggacc
     4441 ctgcacctga ctgtgctgag aatcctgaag cagctggtgg acttccccga ggcactgctg
     4501 ctcccctggc acgaggccgt ggatgcctgc atggcctgcc tgcggtcccc aaacactgat
     4561 cgagaggtgc tccaggaact gattttcttc ctgcaccgcc tgacctcagt gagcagggac
     4621 tatgccgtgg tgctgaatca gctgggagca agagacgcta tctccaaggc cctggaaaag
     4681 cacctgggaa agctggagct ggctcaggag ctgcgggaca tggtgttcaa gtgtgagaag
     4741 catgcccacc tctaccgcaa actcatcacc aacatcctgg gaggctgcat ccagatggtg
     4801 ctgggccaga tcgaagacca cagacgaacc caccaaccca tcaacatccc tttctttgat
     4861 gtgttcctca gatacctgtg ccagggctcc agtgtggaag tgaaggagga caagtgctgg
     4921 gagaaggtgg aggtgtcctc caacccgcac cgggccagca agctgacgga ccacaacccc
     4981 aagacctatt gggagtccaa cggcagcgcc ggctcccact acatcaccct gcacatgcgc
     5041 cggggcatcc tcatcaggca actgactctg cttgtggcta gtgaggactc gagttacatg
     5101 ccggcccgag tggtggtgtg cgggggtgat agcactagct ctcttcacac ggaactcaac
     5161 tcggtgaatg tgatgccctc tgccagccgg gtgatcctcc tggagaacct gacccgcttc
     5221 tggcccatca tccagatccg cataaagcgc tgccagcagg gtggcattga tacgcgcatt
     5281 cgggggttag agatcctagg ccccaagccc acgttctggc cagtgttccg ggagcagctc
     5341 tgtcgtcaca cacgcctctt ctacatggtt cgggcacagg cctggagcca ggacatggca
     5401 gaggaccgca ggagcctcct gcacctgagt tctagactca acggtgctct gcgccaggag
     5461 cagaattttg ctgaccgctt cctccctgat gacgaggctg cccaagctct gggcaagacc
     5521 tgctgggagg ccctggtcag ccccgtggtg cagaacatca cctcccctga tgaggatggc
     5581 attagccccc tgggttggct gctggaccag tacctggagt gtcaggaagc tgtcttcaac
     5641 ccccagagcc gcggcccagc tttcttctcg cgggtgcgcc gtctcactca cctgctggtg
     5701 catgtcgagc cctgtgaggc accccctcct gtggtggcca ctcctcggcc caaaggcaga
     5761 aacagaagcc acgactggag ctccttggct acccggggcc ttccaagcag catcatgaga
     5821 aacctgacgc gctgttggcg ggccgtggtg gagaagcagg tgaacaattt tctgacctca
     5881 tcctggcggg atgatgactt tgtgccacgc tactgtgagc actttaatat tctgcagaac
     5941 tcaagctctg aactgtttgg gcctcgggca gccttcttgc tggcgctgca aaatggctgt
     6001 gcgggagcct tgctgaagct cccttttctc aaagctgccc acgtgagtga gcagttcgcc
     6061 cggcacattg accagcagat ccagggcagc cggatcggtg gagcccagga aatggagagg
     6121 ctggcacagc tgcagcaatg cctgcaagct gtcctgattt tctccggctt ggagatagcc
     6181 accacttttg agcattatta ccagcactac atggcggacc gtctcctggg cgtggtctcg
     6241 agctggctgg agggggccgt gctggagcag atcggtccct gcttccccaa ccgcctcccc
     6301 cagcagatgt tgcagagcct gagcacctct aaggagctgc agcgccagtt ccacgtctac
     6361 cagctccagc agctggatca ggaactcctg aagctggagg atacagagaa gaaaatacag
     6421 gtgggccttg gggccagtgg caaggagcac aagagcgaga aggaagagga agctggggca
     6481 gcagcagtgg tggatgtggc ggagggagag gaggaagagg aggagaatga ggacctctac
     6541 tatgaagggg caatgccaga agtgtctgtg cttgtcctgt cccgacactc ctggcctgtt
     6601 gcctcaatct gccacacact gaaccccaga acctgcctgc cctcctacct gaggggcact
     6661 ttgaacagat actccaactt ctacaacaag agtcagagcc accctgccct tgagcgaggc
     6721 tcacagaggc gactgcagtg gacgtggctg ggctgggctg agctgcagtt tgggaaccag
     6781 accctgcatg tgtccaccgt gcagatgtgg ctactgctgt atctcaacga cctgaaggcg
     6841 gtctctgtgg agagtctgct ggcgttctca gggctctccg cagacatgct caatcaggcg
     6901 attgggcccc tcacctcttc aagaggcccc ctggaccttc acgagcaaaa ggatatacca
     6961 ggaggggtcc tcaagattcg agatggcagc aaggaaccca ggtcgagatg ggacattgtg
     7021 cggctcatcc cacctcagac gtacctgcaa gctgagggtg aagacggcca gaacttggag
     7081 aagagacgga atcttctgaa ctgcctcatc gtccgaatcc tcaaggccca tggagatgag
     7141 gggctgcaca ttgaccagct tgtctgtctg gtgctggagg cttggcagaa gggcccgtgt
     7201 cctcccaggg gtttggtcag cagccttggt aaggggtctg catgcagcag cactgacgtc
     7261 ctctcctgca tcctacacct cctgggcaag ggcacgctga gacgccatga cgaccggccc
     7321 caggtgctgt cctatgcagt ccctgtgact gtcatggagc ctcacactga gtccctgaac
     7381 ccaggctcct caggccccaa cccacccctc accttccata ccctacagat tcgctcccgg
     7441 ggtgtgccct atgcctcctg cactgccacc cagagcttct ctaccttccg gggatccgac
     7501 tacaaagacc atgacggtga ttataaagat catgacatcg actacaagga tgacgatgac
     7561 aagtagcggc cgcgaaggat ctgcgatcgc tccggtgccc gtcagtgggc agagcgcaca
     7621 tcgcccacag tccccgagaa gttgggggga ggggtcggca attgaacggg tgcctagaga
     7681 aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact ggctccgcct ttttcccgag
     7741 ggtgggggag aaccgtatat aagtgcagta gtcgccgtga acgttctttt tcgcaacggg
     7801 tttgccgcca gaacacagct gaagcttcga ggggctcgca tctctccttc acgcgcccgc
     7861 cgccctacct gaggccgcca tccacgccgg ttgagtcgcg ttctgccgcc tcccgcctgt
     7921 ggtgcctcct gaactgcgtc cgccgtctag gtaagtttaa agctcaggtc gagaccgggc
     7981 ctttgtccgg cgctcccttg gagcctacct agactcagcc ggctctccac gctttgcctg
     8041 accctgcttg ctcaactcta cgtctttgtt tcgttttctg ttctgcgccg ttacagatcc
     8101 aagctgtgac cggcgcctac gctagatgac cgagtacaag cccacggtgc gcctcgccac
     8161 ccgcgacgac gtcccccggg ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc
     8221 cacgcgccac accgtcgacc cggaccgcca catcgagcgg gtcaccgagc tgcaagaact
     8281 cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc
     8341 ggtggcggtc tggaccacgc cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg
     8401 cccgcgcatg gccgagttga gcggttcccg gctggccgcg cagcaacaga tggaaggcct
     8461 cctggcgccg caccggccca aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc
     8521 cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga
     8581 gcgcgccggg gtgcccgcct tcctggagac ctccgcgccc cgcaacctcc ccttctacga
     8641 gcggctcggc ttcaccgtca ccgccgacgt cgaggtgccc gaaggaccgc gcacctggtg
     8701 catgacccgc aagcccggtg cctgagtcga caatcaacct ctggattaca aaatttgtga
     8761 aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt
     8821 aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa
     8881 atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt
     8941 gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct
     9001 cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg
     9061 ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc
     9121 ggggaaatca tcgtcctttc cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg
     9181 gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct
     9241 gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc
     9301 cctttgggcc gcctccccgc ctggtacctt taagaccaat gacttacaag gcagctgtag
     9361 atcttagcca ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaacgaa
     9421 aataagatct gctttttgct tgtactgggt ctctctggtt agaccagatc tgagcctggg
     9481 agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc
     9541 ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct
     9601 tttagtcagt gtggaaaatc tctagcagta gtagttcatg tcatcttatt attcagtatt
     9661 tataacttgc aaagaaatga atatcagaga gtgagaggaa cttgtttatt gcagcttata
     9721 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc
     9781 attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg ctctagctat
     9841 cccgccccta actccgccca gttccgccca ttctccgccc catggctgac taattttttt
     9901 tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg
     9961 cttttttgga ggcctagact tttgcagaga cggcccaaat tcgtaatcat ggtcatagct
    10021 gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat
    10081 aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc
    10141 actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg
    10201 cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct
    10261 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
    10321 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
    10381 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
    10441 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
    10501 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
    10561 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
    10621 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
    10681 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
    10741 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
    10801 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt
    10861 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
    10921 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
    10981 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
    11041 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
    11101 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac
    11161 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt
    11221 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt
    11281 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt
    11341 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc
    11401 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa
    11461 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg
    11521 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt
    11581 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc
    11641 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt
    11701 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg
    11761 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac
    11821 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc
    11881 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt
    11941 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg
    12001 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag
    12061 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa
    12121 acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtct aagaaaccat
    12181 tattatcatg acattaacct ataaaaatag gcgtatcacg aggccctttc gtctcgcgcg
    12241 tttcggtgat gacggtgaaa acctctgaca catgcagctc ccggagacgg tcacagcttg
    12301 tctgtaagcg gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg
    12361 gtgtcggggc tggcttaact atgcggcatc agagcagatt gtactgagag tgcaccatat
    12421 gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc gccattcgcc
    12481 attcaggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc tattacgcca
    12541 gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg gtaacgccag ggttttccca
    12601 gtcacgacgt tgtaaaacga cggccagtgc caagctg
//
