LOCUS       V036644                12154 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V036644
VERSION     V036644
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2317..6180
                     /label="Cachd1(NM_001191758)"
                     /note="Cachd1(NM_001191758)"
                     /gene="Cachd1"
     CDS             6187..6252
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        6296..6507
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             6520..6788
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      6817..6826
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             6847..7509
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             7579..7632
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             7633..8232
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    8233..8821
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             8895..9128
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    9200..9321
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      9361..9496
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(9529..9545)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    9553..9569
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(9577..9607)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    9622..9643
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(9931..10519)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(10690..11550)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(11551..11655)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     12129..12145
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc ctgatacgaa ctcggaattc gccaccatgg cccgcgagcc cgaggaagag
     2341 gagaccgtaa ggacggccgc tctggtccgt cgctgtcccc gctgccctgg ctggcctggg
     2401 gccccgcggc cgcccttctg gctgctctgc cttgtggcat gctggatctt gggcgccgtg
     2461 gcggacgccg acttctccat cctggacgag gcgcaagtgc tagcgagcca gatgcggagg
     2521 ctggctgccg aggagctggg ggtggtcacc atgcagagga tcttcaactc cttggtttac
     2581 acagagaaga tctcaaacgg agagagcgaa gttcagcagc tagccaaaaa aatccgggag
     2641 aagttcaacc gttacctgga cgtggtcaat cggaacaagc aagttgtaga agcctcctat
     2701 acagcccacc tgacctcccc gctgaccgcc atccaggact gctgcacaat tccaccctcc
     2761 atgatggagt ttgatgggaa cttcaatacc aatgtgtcta gaacagtgag ctgcgatcga
     2821 ctctctacta ctgtgaacag ccgagccttc aatccaggac gagacttaaa ttcagttctt
     2881 gcagacaacc tgaaatctaa ccctggaatc aagtggcaat atttcagctc agaagaagga
     2941 attttcactg tcttcccagc acacaagttc cggtgtaagg gcagctatga gcaccgcagt
     3001 cggcccatct atgtctctac cgtccggccg cagtccaaac acatagtcgt gatcctggac
     3061 cacggggcct cagtcacgga cacccagctc cagattgcca aggacgccgc tcaggtcatc
     3121 ctcagtgcca ttgatgaaca cgacaagatt tctgtattga ctgtggcaga caccgtcagg
     3181 acctgttcgt tggaccagtg ctataagacg tacctgtctc cagccaccag tgagacaaaa
     3241 aggaaaatgt ccacatttgt tagcagtgtc aaaccctccg acagccccac ccagcacgca
     3301 gtagggtttc atagggcgtt ccagttgatt cgaagcacca gcaataacac tcggttccaa
     3361 gcaaacacag acatggtcat catttacctg tcagcgggta tcacatcaaa ggactcctcg
     3421 gaagaggata aaaaggcaac tctccgcgtc atcaacgaag aaaatggctt cctgaacaac
     3481 tctgtaatga ttcttaccta cgccctcatg aatgatgggg tgactggttt gaaggaactg
     3541 gcgttcctca gggatctggc tgaacagaac tctgggaagt atggaatccc agaccggacg
     3601 gccttgcctg tgattaaggg cagcatgatg gtgctgaacc agctgagcaa cctggagacc
     3661 actgttggca ggttctatac caaccttccc aaccggatga ttgacgaggc cgtcttcagc
     3721 ctccccttct ctgatgagat gggagatggt ctgatcatga ctgtgagtaa accctgttac
     3781 tttggaaacc ttctcctggg gatcgtaggt gtggatgtga atctggctta cattctcgaa
     3841 gatgtgacat attaccaaga ctctttagct tcctatactt ttctcataga tgacaaaggg
     3901 tacacactta tgcacccgtc tctcaccagg ccatacttac tgtcagagcc tccccttcat
     3961 accgacatca tacattatga aaatatcccc aagtttgaat tggttcgaca aaatatccta
     4021 agcctccctc taggcagcca gatcatcaca gtgcctgtta actcgtccct gtcttggcac
     4081 atcaacaagc tgagggagac tggaaaggag gcctacaatg tgagctatgc ctggaagatg
     4141 gtacaggaca cttccttcat tctgtgtatt gtggtgatac aaccagaaat acctgtcaaa
     4201 caattgaaga acctcaacac cgtacccagc agcaagctgc tgtaccacag gctagacctt
     4261 ctgggccaac ccagtgcttg tctccacttc aagcagctgg caacactaga aagtccaacc
     4321 gtcatgctgt ctgccggcag cttttcctcc ccctatgagc acctcagcca gccagagacc
     4381 aagcgcatgg tggagcatta cacggcctat ctcagtgaca acactcgcct cattgctaac
     4441 ccagggctca aattctctgt cagaaacgaa gtaatggcga ccagccacgt cacagatgaa
     4501 tggatgacac aaatggaaat gagcagcctg aacacgtaca ttgtccgccg ttacatagca
     4561 acacccaatg gcgtcctcag aatttatcct ggttccctca tggacaaagc atttgatccc
     4621 acgaggagac agtggtatct tcatgcagta gccaatccag ggctgatttc cttgacgggc
     4681 ccttacctag atgttggagg agctggctat gtcgtgacaa tcagccacac aattcactca
     4741 tccagtaccc agctgtcttc cggacacact gtggctgtga tgggcatcga cttcacactg
     4801 aggtacttct acaaggttct gatggatctc ttaccagttt gtaaccaaga tggtggcaac
     4861 aaaataaggt gcttcataat ggaggacaga ggttatctgg tagctcatcc aaccctcgtt
     4921 gaccccaaag gtcatgcacc tctggaacag caacacatca cccacaagga gcccctggta
     4981 gcaaacgata tcctgaacca tcccaacttt gtgaagaaga acttgtgcaa cagcttcagt
     5041 gaccggacag tgcagagatc ttacaagttc aacaccagcc tggtggggga tttgacaaac
     5101 cttgtgcatg gcagccactg ttctaaatac cgcctgacaa ggatcccagg aaccaacgcg
     5161 tttgtcggca tcgtcaatga gacctgcgac tctcttgcct tctgcgcttg cagcatggtg
     5221 gaccgtctct gtctcaactg tcaccgaatg gagcaaaatg aatgtgaatg tccttgtgag
     5281 tgccccctgg aggtcaatga gtgtactggc aacctcacca atgcagagaa cagaaacccg
     5341 agctgtgagg tccaccagga gccagtgaca tacacagcta tcgatcctgg cctgcaagat
     5401 gccctgcacc agtgtgtcaa cagtcgatgt aaccagagga tggaaagtgg ggactgtttc
     5461 ggagtgctgg actgcgagtg gtgcgtggtg gacagtgacg gaaagactca cctggacaaa
     5521 tcctactgtg cgccccagaa agaatgcttt ggagggatcg tgggagccaa aagtccctac
     5581 gttgatgaca tgggagccat cggtgatgag gtgatcacac tgaacatgat taagagcgcc
     5641 ccagtgggcc ctgtggccgg agggatcatg ggctgcatca tggtgctggt ccttgccgtc
     5701 tacgcttacc gccaccagat ccatcgccgg agccaccagc atatgtctcc tcttgctgct
     5761 caagaaatgt cagtgcgtat gtccaacctg gagaatgaca gagatgaaag agaggatgac
     5821 agtcacgaag acagaggcat catcagcaac actcggttca tagctgcagt catggagcgg
     5881 cacgcacaca gcccggaaag gaggcgacgc tactggggcc gctcgggaac cgaaagtgat
     5941 catgggtaca gcaccatgag cccgcaggaa gacagtgaga accctccatg caacaatgac
     6001 cctttgtcag ctggggtgga tgtagggaac catgatgacg acttggacct ggacaccccg
     6061 cctcagactg cagccctgct gagccacaag ttccaccact accgctcaca ccacccgacc
     6121 ctccatcaca gccaccacct gcaggcggcc gtgacagtgc acacggtgga tgctgagtgc
     6181 ggatccgact acaaagacca tgacggtgat tataaagatc atgacatcga ctacaaggat
     6241 gacgatgaca agtagcggcc gcgaaggatc tgcgatcgct ccggtgcccg tcagtgggca
     6301 gagcgcacat cgcccacagt ccccgagaag ttggggggag gggtcggcaa ttgaacgggt
     6361 gcctagagaa ggtggcgcgg ggtaaactgg gaaagtgatg tcgtgtactg gctccgcctt
     6421 tttcccgagg gtgggggaga accgtatata agtgcagtag tcgccgtgaa cgttcttttt
     6481 cgcaacgggt ttgccgccag aacacagctg aagcttcgag gggctcgcat ctctccttca
     6541 cgcgcccgcc gccctacctg aggccgccat ccacgccggt tgagtcgcgt tctgccgcct
     6601 cccgcctgtg gtgcctcctg aactgcgtcc gccgtctagg taagtttaaa gctcaggtcg
     6661 agaccgggcc tttgtccggc gctcccttgg agcctaccta gactcagccg gctctccacg
     6721 ctttgcctga ccctgcttgc tcaactctac gtctttgttt cgttttctgt tctgcgccgt
     6781 tacagatcca agctgtgacc ggcgcctacg ctagacgcca ccatggagag cgacgagagc
     6841 ggcctgcccg ccatggagat cgagtgccgc atcaccggca ccctgaacgg cgtggagttc
     6901 gagctggtgg gcggcggaga gggcaccccc aagcagggcc gcatgaccaa caagatgaag
     6961 agcaccaaag gcgccctgac cttcagcccc tacctgctga gccacgtgat gggctacggc
     7021 ttctaccact tcggcaccta ccccagcggc tacgagaacc ccttcctgca cgccatcaac
     7081 aacggcggct acaccaacac ccgcatcgag aagtacgagg acggcggcgt gctgcacgtg
     7141 agcttcagct accgctacga ggccggccgc gtgatcggcg acttcaaggt ggtgggcacc
     7201 ggcttccccg aggacagcgt gatcttcacc gacaagatca tccgcagcaa cgccaccgtg
     7261 gagcacctgc accccatggg cgataacgtg ctggtgggca gcttcgcccg caccttcagc
     7321 ctgcgcgacg gcggctacta cagcttcgtg gtggacagcc acatgcactt caagagcgcc
     7381 atccacccca gcatcctgca gaacgggggc cccatgttcg ccttccgccg cgtggaggag
     7441 ctgcacagca acaccgagct gggcatcgtg gagtaccagc acgccttcaa gacccccatc
     7501 gccttcgcca gatcccgcgc tcagtcgtcc aattctgccg tggacggcac cgccggaccc
     7561 ggctccaccg gatctcgcga gggcagagga agtcttctaa catgcggtga cgtggaggag
     7621 aatcccggcc ctatgaccga gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc
     7681 cccagggccg tacgcaccct cgccgccgcg ttcgccgact accccgccac gcgccacacc
     7741 gtcgatccgg accgccacat cgagcgggtc accgagctgc aagaactctt cctcacgcgc
     7801 gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg gcgccgcggt ggcggtctgg
     7861 accacgccgg agagcgtcga agcgggggcg gtgttcgccg agatcggccc gcgcatggcc
     7921 gagttgagcg gttcccggct ggccgcgcag caacagatgg aaggcctcct ggcgccgcac
     7981 cggcccaagg agcccgcgtg gttcctggcc accgtcggcg tctcgcccga ccaccagggc
     8041 aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg cggccgagcg cgccggggtg
     8101 cccgccttcc tggagacctc cgcgccccgc aacctcccct tctacgagcg gctcggcttc
     8161 accgtcaccg ccgacgtcga ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag
     8221 cccggtgcct gaaatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt
     8281 aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct
     8341 attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt
     8401 tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac
     8461 gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct
     8521 ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca
     8581 ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaaatc atcgtccttt
     8641 ccttggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc
     8701 ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct
     8761 cttccgcgtc ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg
     8821 cctggtacct ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa
     8881 agaaaagggg ggactggaag ggctaattca ctcccaacga agataagatc tgctttttgc
     8941 ttgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg
     9001 gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg
     9061 tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat
     9121 ctctagcagt agtagttcat gtcatcttat tattcagtat ttataacttg caaagaaatg
     9181 aatatcagag agtgagagga acttgtttat tgcagcttat aatggttaca aataaagcaa
     9241 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc
     9301 caaactcatc aatgtatctt atcatgtctg gctctagcta tcccgcccct aactccgccc
     9361 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt
     9421 tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga
     9481 ggcttttttg gaggcctaga cttttgcaga gaccaaattc gtaatcatgt catagctgtt
     9541 tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa
     9601 gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact
     9661 gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc
     9721 ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg
     9781 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
     9841 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
     9901 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
     9961 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
    10021 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
    10081 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
    10141 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
    10201 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
    10261 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
    10321 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt
    10381 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
    10441 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
    10501 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
    10561 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
    10621 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
    10681 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
    10741 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
    10801 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc
    10861 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
    10921 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
    10981 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
    11041 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
    11101 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
    11161 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
    11221 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
    11281 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
    11341 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
    11401 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac
    11461 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
    11521 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
    11581 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
    11641 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat
    11701 tatcatgaca ttaacctata aaaataggcg tatcacgagg ccctttcgtc tcgcgcgttt
    11761 cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca cagcttgtct
    11821 gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg
    11881 tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc accatatgcg
    11941 gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc attcgccatt
    12001 caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat tacgccagct
    12061 ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt tttcccagtc
    12121 acgacgttgt aaaacgacgg ccagtgccaa gctg
//
