LOCUS       V042765                 9835 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V042765
VERSION     V042765
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1920
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2010..2213
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2302..4689
                     /label="Cdh11(NM_053392)"
                     /note="Cdh11(NM_053392)"
                     /gene="Cdh11"
     CDS             4696..4761
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        4805..5016
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             5029..5297
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     CDS             5324..5923
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    5930..6518
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             6592..6825
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    6897..7018
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      7037..7172
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(7210..7226)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    7234..7250
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(7258..7288)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    7303..7324
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(7612..8200)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(8371..9231)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(9232..9336)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     9810..9826
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt
     1921 atcgatacta gtattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
     1981 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
     2041 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
     2101 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
     2161 tgacgcaaat gggcggtagg cgtgtacggt gggaggttta tataagcaga gctcgtttag
     2221 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagattct
     2281 agagctagcg aattcgccac catgaaggag aactactgtt tacaagctgc cctggtgtgc
     2341 ctgagcatgc tataccacag ccaggcattt gccctggagc gacgaagcca cctgcgtccc
     2401 tcattccatg gacatcatga gaagggcaag gagggacagg tgctgcaacg ctccaagaga
     2461 ggttgggtct ggaaccagtt ctttgtgata gaagagtaca ccgggcctga ccctgtgctt
     2521 gtgggcaggc ttcattctga cattgactct ggtgatggga acattaaata cattctctca
     2581 ggtgaaggag caggaaccat ttttgtgatt gatgacaaat cagggaacat tcacgccacc
     2641 aagacactgg accgagagga gagagcccag tatacactga tggctcaggc ggtggacagg
     2701 gacaccaaca ggccattgga accaccttca gaatttatcg ttaaggtcca ggacattaat
     2761 gacaaccctc cagagtttct gcatgaaacc tatcatgcca atgtgcctga gaggtccaat
     2821 gtgggaactt cagtaatcca agtgacagcc tctgatgcag atgatcccac ctatggaaat
     2881 agtgccaagt tagtgtatag tatccttgaa ggacaaccct acttctcagt ggaggcccaa
     2941 acaggtatca tcagaacagc ccttcccaat atggacagag aagccaagga ggagtaccat
     3001 gtggtgatcc aggccaagga catgggtgga cacatgggcg gactctcagg gacaaccaaa
     3061 gtgacgatca cactgactga tgtcaatgac aacccaccaa agtttccaca gagcgtgtac
     3121 cagatgtctg tttcagaagc agctgtcccg ggggaggaag taggaagggt gaaggctaaa
     3181 gacccagaca tcggagaaaa tggcttagtc acatacaata tcgttgatgg agatggcatg
     3241 gaactgtttg aaatcacaac agactatgaa acacaggatg gtgtggtgaa gctgaaaaag
     3301 cctgtagatt ttgaaaccaa aagagcatat agcttgaagg tagaggcggc caatgtacac
     3361 attgatccga agttcatcag caatggacct ttcaaggaca cagtgactgt caagattgca
     3421 gtagaagatg ccgacgagcc tcccatgttc ttggccccaa gttatatcca tgaagttcaa
     3481 gaaaatgcag ctgctggcac tgtggttggg agagtacatg ccaaagaccc ggatgctgcc
     3541 aacagcccaa taaggtattc aattgatcgt catactgacc tcgacaggtt tttcacgatt
     3601 aatccagaag acggttttat taaaactacg aaacctctag atagggaaga aactgcctgg
     3661 ctcaacatct ctgtctttgc agcagaaatc cacaacagac atcaggaagc caaagtccca
     3721 gtggccatca gggtcctgga tgtcaatgac aatgctccta agtttgctgc cccttatgaa
     3781 ggctttatct gtgagagtga tcaccccaaa ccactctcca accagccaat agttacagtt
     3841 agtgcagatg acctggatga cacagccaat ggaccaagat ttatcttcag tctaccccct
     3901 gaaatcattc acaacccaaa tttcacagta agagacaaca gagataacac tgcaggagta
     3961 tatgcccgac gtggagggtt cagtcggcag aagcaggacc tctacctcct gcccattgtg
     4021 attagcgatg gtggcatccc acctatgagt agcaccaata ccctcactat aaaagtctgt
     4081 gggtgtgatg tgaatggggc actgttgtcc tgtaacgctg aagcctacat cctgaatgcc
     4141 ggtctgagca ctggggcatt gatcgccatc cttgcctgca tcgtcattct tctggtcatc
     4201 gttgtgttgt ttgtgaccct gaggaggcaa aagaaagaac cactcattgt atttgaagaa
     4261 gaggatgtcc gggagaacat cataacctat gatgatgaag ggggtggtga ggaagacacc
     4321 gaagccttcg acattgccac cctgcagaat cctgacggta tcaatggatt tatccctcgc
     4381 aaagatatca aacctgagta tcagtacatg cctagacctg ggctgcgacc agcacccaac
     4441 agtgtggatg tggacgactt catcaacaca agaatacagg aggccgataa tgatcccacg
     4501 gcccctccct atgactccat ccaaatctat ggttatgagg gcaggggttc cgtggctggg
     4561 tccctgagct ccttggagtc tgccaccaca gattcggacc tggactacga ctatctacag
     4621 aactggggac ctcgttttaa gaaattagca gacttgtatg gctccaaaga cacttttgat
     4681 gatgactctg gatccgacta caaagaccat gacggtgatt ataaagatca tgacatcgac
     4741 tacaaggatg acgatgacaa gtagcggccg cgaaggatct gcgatcgctc cggtgcccgt
     4801 cagtgggcag agcgcacatc gcccacagtc cccgagaagt tggggggagg ggtcggcaat
     4861 tgaacgggtg cctagagaag gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg
     4921 ctccgccttt ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac
     4981 gttctttttc gcaacgggtt tgccgccaga acacagctga agcttcgagg ggctcgcatc
     5041 tctccttcac gcgcccgccg ccctacctga ggccgccatc cacgccggtt gagtcgcgtt
     5101 ctgccgcctc ccgcctgtgg tgcctcctga actgcgtccg ccgtctaggt aagtttaaag
     5161 ctcaggtcga gaccgggcct ttgtccggcg ctcccttgga gcctacctag actcagccgg
     5221 ctctccacgc tttgcctgac cctgcttgct caactctacg tctttgtttc gttttctgtt
     5281 ctgcgccgtt acagatccaa gctgtgaccg gcgcctacgc tagatgaccg agtacaagcc
     5341 cacggtgcgc ctcgccaccc gcgacgacgt cccccgggcc gtacgcaccc tcgccgccgc
     5401 gttcgccgac taccccgcca cgcgccacac cgtcgacccg gaccgccaca tcgagcgggt
     5461 caccgagctg caagaactct tcctcacgcg cgtcgggctc gacatcggca aggtgtgggt
     5521 cgcggacgac ggcgccgcgg tggcggtctg gaccacgccg gagagcgtcg aagcgggggc
     5581 ggtgttcgcc gagatcggcc cgcgcatggc cgagttgagc ggttcccggc tggccgcgca
     5641 gcaacagatg gaaggcctcc tggcgccgca ccggcccaag gagcccgcgt ggttcctggc
     5701 caccgtcggc gtctcgcccg accaccaggg caagggtctg ggcagcgccg tcgtgctccc
     5761 cggagtggag gcggccgagc gcgccggggt gcccgccttc ctggagacct ccgcgccccg
     5821 caacctcccc ttctacgagc ggctcggctt caccgtcacc gccgacgtcg aggtgcccga
     5881 aggaccgcgc acctggtgca tgacccgcaa gcccggtgcc tgagtcgaca atcaacctct
     5941 ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct
     6001 atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat
     6061 tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt
     6121 caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat
     6181 tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc
     6241 ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga
     6301 caattccgtg gtgttgtcgg ggaaatcatc gtcctttcct tggctgctcg cctgtgttgc
     6361 cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga
     6421 ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc gccttcgccc
     6481 tcagacgagt cggatctccc tttgggccgc ctccccgcct ggtaccttta agaccaatga
     6541 cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga ctggaagggc
     6601 taattcactc ccaacgaaaa taagatctgc tttttgcttg tactgggtct ctctggttag
     6661 accagatctg agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat
     6721 aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact
     6781 agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtagt agttcatgtc
     6841 atcttattat tcagtattta taacttgcaa agaaatgaat atcagagagt gagaggaact
     6901 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata
     6961 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc
     7021 atgtctggct ctagctatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca
     7081 tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt
     7141 ccagaagtag tgaggaggct tttttggagg cctagacttt tgcagagacg gcccaaattc
     7201 gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa
     7261 catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac
     7321 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca
     7381 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc
     7441 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
     7501 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
     7561 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
     7621 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
     7681 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
     7741 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
     7801 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
     7861 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
     7921 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
     7981 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
     8041 ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
     8101 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
     8161 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
     8221 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
     8281 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta
     8341 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat
     8401 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac
     8461 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg
     8521 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag
     8581 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt
     8641 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt
     8701 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt
     8761 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt
     8821 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct
     8881 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt
     8941 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac
     9001 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa
     9061 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa
     9121 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca
     9181 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct
     9241 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga
     9301 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc
     9361 tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag
     9421 gccctttcgt ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca tgcagctccc
     9481 ggagacggtc acagcttgtc tgtaagcgga tgccgggagc agacaagccc gtcagggcgc
     9541 gtcagcgggt gttggcgggt gtcggggctg gcttaactat gcggcatcag agcagattgt
     9601 actgagagtg caccatatgc ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg
     9661 catcaggcgc cattcgccat tcaggctgcg caactgttgg gaagggcgat cggtgcgggc
     9721 ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat taagttgggt
     9781 aacgccaggg ttttcccagt cacgacgttg taaaacgacg gccagtgcca agctg
//
