LOCUS       V031559                10228 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V031559
VERSION     V031559
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2317..4254
                     /label="Crtac1(NM_145123)"
                     /note="Crtac1(NM_145123)"
                     /gene="Crtac1"
     CDS             4261..4326
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        4370..4581
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             4594..4862
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      4891..4900
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             4921..5583
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             5653..5706
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             5707..6306
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    6307..6895
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             6969..7202
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    7274..7395
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      7435..7570
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(7603..7619)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    7627..7643
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(7651..7681)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    7696..7717
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8005..8593)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(8764..9624)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(9625..9729)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     10203..10219
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc ctgatacgaa ctcggaattc gccaccatgg ctccgagcgc tgacccgggc
     2341 atggtcagga tggctttgct cctgctgccg cccctgtggc ttctgcctct cactggggga
     2401 tcccagaggg ctgagcccat gttcactgca gtcaccaact cagtcctgcc ccctgactat
     2461 gacagcaacc ccacccagct caactatgga gtggcagtga cagatgtgga ccatgatggg
     2521 gacttcgaga tcgttgtggc ggggtacacc ggccccaacc tggttctgaa gtacaatcga
     2581 gcccagaatc ggctggtgaa tattgcggtg gacgagcgca gctcacccta ctatgctctg
     2641 agggaccgtc aggggaacgc catcggggtc acagcctgtg acatcgatgg ggacggccgt
     2701 gaggagatct actttctcaa caccaataac gccttctcag gtgtggccac atacacagac
     2761 aagttgttca aattccgaaa taaccggtgg gaagacatcc tgagtgatga cgtcaatgtg
     2821 gcccgtggag tggccagcct ctttgcggga cgctccgtgg cctgtgtaga caggacgggc
     2881 tctggaagat actctatcta catagccaac tatgcctatg gtgatgtggg gcctgatgcc
     2941 ctcatcgaaa tggaccctga ggccagtgac ctgtcccggg ggatcctggc actcagggac
     3001 gtggcggctg aggctggggt cagcaagtac acagcgggcc ggggtgtcag cgtaggcccc
     3061 attctcagca gcagtgcctc agatatcttc tgtgacaacg agaacgggcc caacttcctc
     3121 ttccacaacc aaggcaacgg tacctttgtg gatactgcgg ccagtgccgg cgtagacgac
     3181 cctcatcagc atggccgagg tgtggccctg gcagatttca accgtgacgg caaagtagac
     3241 atcgtctatg gcaactggaa cggcccacat cgcctctatc tacagatgag tgctcacggg
     3301 aaggtccggt ttcgggacat tgcttcgccc aagttctcca cgccctcccc tgtccggact
     3361 gtcattgctg ccgactttga caatgaccag gaactggaag tcttcttcaa caacattgcc
     3421 tacagaagct cctcagccaa tcgcctcttc cgtgtcatcc gcagggagca cggggaccct
     3481 ctcatcgagg agctcaatcc tggtgacgcc ctagagcctg agggccgggg tacagggggc
     3541 gtagtgaccg acttcgatgg tgatgggatg ctggatctca tcttgtcaca tggagagtcc
     3601 atggctcagc cactgtctgt cttccgggga aatcagggct tcagcaacaa ctggttgcgt
     3661 gtggtacctc gcacccggtt cggggccttt gccaggggcg ccaaggttgt actctacacc
     3721 aagaagagtg gggcgcacct acggatcatt gatgggggtt ccggctacct gtgtgaaatg
     3781 gagcctgtgg cacattttgg cctgggacgg gatgaagcca gcagtgtgga ggtgacgtgg
     3841 ccagatggca agatggtgag ccgaagtgtg gccagcgagg agatgaactc ggtgttggag
     3901 atcctctacc cccaggatga ggacaaactt cagaacacag ccccactaga gtgcggccaa
     3961 ggattctccc agcaggacaa tggccattgc atggacacca atgaatgcat ccagttccca
     4021 tttgtgtgcc ctcgagacaa acccgtatgt gtcaacacct atggaagcta caggtgccgg
     4081 accaataaaa gatgcaatcg gggctatgaa cccaatgaag acggcacagc ctgtgtggct
     4141 caagtggcct ttttaggtgg gtactcatcg gctgccttta gactctctga gcctctctct
     4201 caggcctcgt atctttctct aggcctggga ctttgccttc agttatatgc acttggatcc
     4261 gactacaaag accatgacgg tgattataaa gatcatgaca tcgactacaa ggatgacgat
     4321 gacaagtagc ggccgcgaag gatctgcgat cgctccggtg cccgtcagtg ggcagagcgc
     4381 acatcgccca cagtccccga gaagttgggg ggaggggtcg gcaattgaac gggtgcctag
     4441 agaaggtggc gcggggtaaa ctgggaaagt gatgtcgtgt actggctccg cctttttccc
     4501 gagggtgggg gagaaccgta tataagtgca gtagtcgccg tgaacgttct ttttcgcaac
     4561 gggtttgccg ccagaacaca gctgaagctt cgaggggctc gcatctctcc ttcacgcgcc
     4621 cgccgcccta cctgaggccg ccatccacgc cggttgagtc gcgttctgcc gcctcccgcc
     4681 tgtggtgcct cctgaactgc gtccgccgtc taggtaagtt taaagctcag gtcgagaccg
     4741 ggcctttgtc cggcgctccc ttggagccta cctagactca gccggctctc cacgctttgc
     4801 ctgaccctgc ttgctcaact ctacgtcttt gtttcgtttt ctgttctgcg ccgttacaga
     4861 tccaagctgt gaccggcgcc tacgctagac gccaccatgg agagcgacga gagcggcctg
     4921 cccgccatgg agatcgagtg ccgcatcacc ggcaccctga acggcgtgga gttcgagctg
     4981 gtgggcggcg gagagggcac ccccaagcag ggccgcatga ccaacaagat gaagagcacc
     5041 aaaggcgccc tgaccttcag cccctacctg ctgagccacg tgatgggcta cggcttctac
     5101 cacttcggca cctaccccag cggctacgag aaccccttcc tgcacgccat caacaacggc
     5161 ggctacacca acacccgcat cgagaagtac gaggacggcg gcgtgctgca cgtgagcttc
     5221 agctaccgct acgaggccgg ccgcgtgatc ggcgacttca aggtggtggg caccggcttc
     5281 cccgaggaca gcgtgatctt caccgacaag atcatccgca gcaacgccac cgtggagcac
     5341 ctgcacccca tgggcgataa cgtgctggtg ggcagcttcg cccgcacctt cagcctgcgc
     5401 gacggcggct actacagctt cgtggtggac agccacatgc acttcaagag cgccatccac
     5461 cccagcatcc tgcagaacgg gggccccatg ttcgccttcc gccgcgtgga ggagctgcac
     5521 agcaacaccg agctgggcat cgtggagtac cagcacgcct tcaagacccc catcgccttc
     5581 gccagatccc gcgctcagtc gtccaattct gccgtggacg gcaccgccgg acccggctcc
     5641 accggatctc gcgagggcag aggaagtctt ctaacatgcg gtgacgtgga ggagaatccc
     5701 ggccctatga ccgagtacaa gcccacggtg cgcctcgcca cccgcgacga cgtccccagg
     5761 gccgtacgca ccctcgccgc cgcgttcgcc gactaccccg ccacgcgcca caccgtcgat
     5821 ccggaccgcc acatcgagcg ggtcaccgag ctgcaagaac tcttcctcac gcgcgtcggg
     5881 ctcgacatcg gcaaggtgtg ggtcgcggac gacggcgccg cggtggcggt ctggaccacg
     5941 ccggagagcg tcgaagcggg ggcggtgttc gccgagatcg gcccgcgcat ggccgagttg
     6001 agcggttccc ggctggccgc gcagcaacag atggaaggcc tcctggcgcc gcaccggccc
     6061 aaggagcccg cgtggttcct ggccaccgtc ggcgtctcgc ccgaccacca gggcaagggt
     6121 ctgggcagcg ccgtcgtgct ccccggagtg gaggcggccg agcgcgccgg ggtgcccgcc
     6181 ttcctggaga cctccgcgcc ccgcaacctc cccttctacg agcggctcgg cttcaccgtc
     6241 accgccgacg tcgaggtgcc cgaaggaccg cgcacctggt gcatgacccg caagcccggt
     6301 gcctgaaatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat
     6361 gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct
     6421 tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag
     6481 gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc
     6541 cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc
     6601 ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct
     6661 cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg
     6721 ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg
     6781 gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg
     6841 cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcctggt
     6901 acctttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa
     6961 ggggggactg gaagggctaa ttcactccca acgaagataa gatctgcttt ttgcttgtac
     7021 tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc
     7081 actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt
     7141 gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag
     7201 cagtagtagt tcatgtcatc ttattattca gtatttataa cttgcaaaga aatgaatatc
     7261 agagagtgag aggaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat
     7321 cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact
     7381 catcaatgta tcttatcatg tctggctcta gctatcccgc ccctaactcc gcccatcccg
     7441 cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt
     7501 tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt
     7561 tttggaggcc tagacttttg cagagaccaa attcgtaatc atgtcatagc tgtttcctgt
     7621 gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa
     7681 agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc
     7741 tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag
     7801 aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt
     7861 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
     7921 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
     7981 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
     8041 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
     8101 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
     8161 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
     8221 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
     8281 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
     8341 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
     8401 tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat
     8461 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
     8521 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
     8581 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
     8641 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
     8701 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga
     8761 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc
     8821 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg
     8881 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat
     8941 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat
     9001 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg
     9061 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc
     9121 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa
     9181 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc
     9241 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt
     9301 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag
     9361 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt
     9421 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag
     9481 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac
     9541 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc
     9601 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca
     9661 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg
     9721 ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc taagaaacca ttattatcat
     9781 gacattaacc tataaaaata ggcgtatcac gaggcccttt cgtctcgcgc gtttcggtga
     9841 tgacggtgaa aacctctgac acatgcagct cccggagacg gtcacagctt gtctgtaagc
     9901 ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg
     9961 ctggcttaac tatgcggcat cagagcagat tgtactgaga gtgcaccata tgcggtgtga
    10021 aataccgcac agatgcgtaa ggagaaaata ccgcatcagg cgccattcgc cattcaggct
    10081 gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa
    10141 agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg
    10201 ttgtaaaacg acggccagtg ccaagctg
//
