LOCUS       V032003                15880 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V032003
VERSION     V032003
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2317..9906
                     /label="Cul9(NM_001081335)"
                     /note="Cul9(NM_001081335)"
                     /gene="Cul9"
     CDS             9913..9978
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        10022..10233
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             10246..10514
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      10543..10552
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             10573..11235
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             11305..11358
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             11359..11958
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    11959..12547
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             12621..12854
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    12926..13047
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      13087..13222
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(13255..13271)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    13279..13295
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(13303..13333)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    13348..13369
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(13657..14245)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(14416..15276)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(15277..15381)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     15855..15871
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc ctgatacgaa ctcggaattc gccaccatgg taggggaacg gcgtgcaggg
     2341 aaccttctgg tacccttggg gcctcggctg caggcgtacc ctgaagaact ccttcgacag
     2401 aggcctggac atgatggcca tcccgagtac ctgatccgct ggagtgtcct caagtgtagc
     2461 gaagagggca aagtgagctc agaggaggag aagacagagc atctcctcat gtggctgtca
     2521 gccccagagg tctacgccaa ctgccccatg ctactgaggg agcggccaat acctaaaggc
     2581 ccgcagcacg aacctgctgg agaggtggga agcttcccac gagaccctgg aggcctggat
     2641 gagttggcaa tgacagagat ggaggctgat gtccgggcgc tggtgcgcag ggctgccagg
     2701 cagctggcgg agggtgggac ctcgagcctg acggctgctg tgcttcacac cgtccacgtg
     2761 ctcagtgcct atgccagcat cgggcctctc accggagtct tcagggagac cggagcccta
     2821 gatctgctca tgcacatgct gtgcaatccc gagcctcaga tccgccggag tgcaggcaag
     2881 atgctccagg ctctggcagc ccatgatgct gggagccggg ctcacgtcct tctgtcactg
     2941 agccagcacg acggcatcga gcagcacatg gattttgaca gccgctacac tttactggag
     3001 ctgtttgcgg agaccacgtc cacagaggag cactgcatgg ccttggaggg catccacctt
     3061 cctcagatcc ctgggaagct gctcttctcc ctggtaaagc gctacctctg tgtcacctcc
     3121 ctgctggatc agctgaataa cagcccagag tcaggagctg gggaccagag ctccccgtgt
     3181 ctcacaaagg agaagagccg ggcgcagcag gagctggagt tcagtatggc cgtgggcaat
     3241 ctcatttctg agctggtgcg gagcatgggc tgggcacgga acctcagtga gcagggcgct
     3301 ttgccgccca ggccatcccg atccatcttc cagccatgcc tctcaggccc tccgcctctg
     3361 ctgctaccca ccatcgtcac tacacccagg aagcaagggc gggccttccg cggtcgttct
     3421 gagttttcta gccgcagcgg ttatggtgaa tacgttcagc agacggtgca gcccggcatg
     3481 cgggttcgca tcctggatga ctacgaggag atcagtgctg gggacgaggg cgagttccag
     3541 cagagcaaca atggcgtgcc ccctgtgcag gtcttctggc agtcgactgg ccgcacctac
     3601 tgggtacact ggcacatgct agagatcctg gggccggagg aagctactgg ggattcagcg
     3661 tccgcagctg tggagaaggg agcaggggca gctgtactgg gcacagtgtg tccctcctgg
     3721 gactggaagc ccacacatgg cctgtactca ctgccgtatc tccagcccga accccagaag
     3781 aacgaggagg agtcagggta cctgacccag gcagagtggt gggagctact gttcttcatc
     3841 aagaagttgg acgtgggtga acagcagcca attttccaga atctgcatga gaaactggat
     3901 gaggaggccc tgggagaaaa ggccctggga gagctctctg tgcccataga gatggctgag
     3961 ggtgtactac aggttctcag caaccgcttt gagggcagca acctctgcga cctgctcaat
     4021 tcccgcatct acactaagta cgggctactg cccaaggaac tggggagctt gccgtcttca
     4081 cggcgtcact cctgtagccc agagccagag gagggctcaa ggtcagctgc caacttctca
     4141 gagaaggaag aggcctgcag agccaatgct gtgcccccag aggcagagat ggagcttgcc
     4201 agtgcaaaga cagagccccc caaagcacag agtgactccc agctgtttaa tcagcttctg
     4261 gtgactgagg ggatgaccct gccccctgaa acacaggagg cagctactga aatggccaga
     4321 gccttgcggg gtcctggtcc gcgcagctcc ctggatcagc atgtggcagc ggtcttggcc
     4381 actgtgcaga tctccagcct ggatacgaac ctgcagctat cagggctctg tgccctctcc
     4441 caggctgtgg aggaggtcac tgagcgagac caccccctgg tccgtcctga cagaccgttg
     4501 agagagaagc tcgtgaagac cctggtggat ttgctaacca accaggtggg agagaagatg
     4561 gtggtggtga tggccctgcg cctcctctac ctgctcatga ccaagcatga atggcgtccg
     4621 ctctttgcca gggagggtgg catctacgct gtgctgattt gcatgcaaga atacaagaca
     4681 tccgtcttgg tgcagcaggc aggactggca gcactcaaga tgctggccat tgctaattcc
     4741 tccgacgtcc ccactttcat ccccagccga gattccatcc aacctctgtt tgatacccag
     4801 atgaccagag agatctttgc cagcatcgac tctgccaccc gcccaggctc cgagagtttg
     4861 ctcctcagtg tacctgcggc tgtggtcctg atgctgaaca ctgaggggtg ctcctctgca
     4921 gtgagaaacg gcctgcttct tctcaacctg cttctatgca accaccacac tttgggagac
     4981 cagatcataa cgcaggagct gagagacacc ttgttcaggc actcggggat agcgccgggg
     5041 acagagccaa tgcccaccac acgcaccatc ctcaccatgc tcctcagtcg ctactcagag
     5101 ccacggggaa gtcctgagcg agcagtactg gagacaccaa gcacccaggg tcaggatggg
     5161 tcccccgagt cgctcatccg gtccctggtg ggtgacctgt cagcagaact cttccttgac
     5221 ttggaacgcg tgctgtgcca tgaaggcagc cctccagctg ccgtgaggcc cctcctcagg
     5281 cgcctccagc aggaaacgca gccattcctc ctgttgctgc ggactctaga tgcccccggg
     5341 cccaacagaa ctctgctgct cacgatactc agggtcatga cccgactgct ggatcatcct
     5401 gagacaatgg tcctcccctg gcacgaggtc ttggagccct gcctcaactg cctgaatggg
     5461 ccaagcagtg actctgaggt tgtccaggag gtgacctgct tcctgcaccg cctggctctg
     5521 atgcagaaag actacgcagt ggtgctttgc tgcctgggag gtaaggaagc cctctccaaa
     5581 gtcctggaca agcactccac tcagctcctg ctggcctctg agctgcgcca cctggtggcc
     5641 gagtgtgaga agtactcgca gctctgcagc aacctcacct cgagcatcct ggcaggctgc
     5701 atccagatgg tgctgggcca gattgaagac cacaggcgaa cctacaagcc catcactatc
     5761 cccttctttg atgtgttcct caggcatctc tgccagggct ccagtgtgga ggtgaaggag
     5821 gacaggtgct gggagaaagt ggaggtgtcc tcgaacccac accgggccag caagctgacg
     5881 gaccgcaacc ccaagaccta ctgggagtcc aacggcagca ctggctccca ctccatcacc
     5941 ttacacatgc accgtggtgt tcttattagg cagctgaccc tgctggtggc cagtgaagat
     6001 tcaagttaca tgccagccag ggtggtggtg tttgggggtg acaatgttgg ctgcatcagc
     6061 acagaactta acacggtaaa cgtgatgccc tctgccagcc gggtgaccct cctggagaac
     6121 ctgacccgct tctggcccat catccaaatc cgaataaagc gctgccagca gggtggtatc
     6181 gacactcggg ttcggggtgt ggaggtgctg ggccccaagc ccacattctg gccattgttc
     6241 cgggagcaac tatgccgacg tacttgtctc ttctacacaa ttcgggcaca agcatggagc
     6301 cgggacattg cagaggaccg ccagcgcctc ctacagctct atccgagact gaacagagtt
     6361 ttgcgccatg aacagaattt tgccgatcgc tttctccctg atgacgaggc cgcccaagca
     6421 ctgggcaaga cctgctggga ggcgctggtc agccccctgg tgcagaacat cacgtctccc
     6481 gatgcaggag gtgtgagctc cctggggtgg ctgctggacc agtacctgga acacagggag
     6541 agtacgcgga gccgccaggg tcctgcagct tcctttgctt cccaagttcg gcgcctgtgc
     6601 cacttgctgg tacatgtgga agcccctcct gggccttctc ctgagccatc ctcgcagccc
     6661 ctcagcaaga acagtaaggg tcaggatgga agccccacac cggcacctac tccggtctgt
     6721 cccagcacca gccttcggaa cctcacccag tgctggctga gtgtggtgca gggacaggtc
     6781 agcagattcc tggccgcagc ctggagggcc tcagactttg tgccccgtta ctgtagtctc
     6841 tatcaacgct tgcaaagcgc gggctcagag ctgttcgggc cgcgggcagc cttcactctg
     6901 gctctgcgca gtggcttctc cggagctctg ctgcagcagt ccttcctcac ggctgctcac
     6961 ataagcgaac agtttgctag gcacattgac caacaaatcc acggtggttt gcttggcgga
     7021 gcggctggag tggggatgct ggggcggctc cagcggcacc tagagcccat catggtcctt
     7081 tctggcctag agctggccac gacgtttgag cacttctatc agcattacat ggctgaccgt
     7141 ctcctgagct tgggctcgag ctggctggag ggggctgtgc tggagcagat cggtccctgc
     7201 ttccccaacc gcctcccgca gctgatgctg caaagcctgc acacctcaga ggagctgcag
     7261 catcgcttcc acctcttcca gctgcagcag ctcgacaggc agctcttgga gcagggagac
     7321 caggaggaat ggaggccaga gaaagtggag gaagatgatg agggacagga gactggaaga
     7381 gaactcttta cagacccagg accagccatt tccgtcctgg tcctgtcacc gcgctgctgg
     7441 ccggtctccc cactgtgcta tctgcaccac cccagaaagc acctacaggc ggagctctgc
     7501 gatgccctgg agcgcttctc cagcttctac agccacagtc agaactgccc agtcttggac
     7561 ataggaccac accggcgact tcagtggaca tggctgggcc gggctgagct gcagtttggg
     7621 gaccagaccc tgcacgtgtc cactgtacag atgtggctgc tgctgaattt taaccaatca
     7681 gaggaggtgt cagtagagac cttgttgagg aattctgacc tctccccaga gctcctgcac
     7741 caggcccttc tgcccctcac ctctgacaat ggtcctttaa cactggagga gactcaggac
     7801 tatccacagg gcggggtgct gcggcttcgt gagcctaggt cccagaccca tgaagaggtc
     7861 ctgtggctga tacctcccca gacgtatctc agtgtagaaa aggatgaggg ccggaccttg
     7921 gaacagaaga ggaacctctt gagctgcctt cttgtccgga tcctcaaagc ccacagggag
     7981 aaaggcctgc acatcgatca gctggtttgt ctggtactgg aggcctggca gaagggtcca
     8041 gatccacctg ggcgcctagg caacgctgcg gcagtgggtg tggcctgctc cagcactgat
     8101 gttctctctt gcatcctgca tctcctgggc cagggctatg tggaacggcg tgatgacagg
     8161 ccccaggtcc tgatgtatgc cactccagag cccatggggc cctgccgggg tcaggcagat
     8221 gtcccctttt gtggcaacaa gaacactgag acctccaggc ctagcccaga agctgtggtg
     8281 gccctggcat ctctacagct gcctgcaggc cgtaccatga gtccccagga ggtagaagga
     8341 ttgatggagc agacggtgcg gcaggtgcag gagacgctga acttggagcc agatgtagct
     8401 cagcaccttc tggctcattc ccactggggc acagagcagc tgctgcagag ctacagcgat
     8461 gacccagaac cactgctcct tgcggcaggg ctgcgagtac cccaggccca ggtggtcccc
     8521 actcgccctg accaatgccc tgtgtgcgtc acccccttag gaccccatga cgactcgccc
     8581 tccctctgct gcctgcactg ctgctgtaag tcttgctgga acgagtacct gacaactcgg
     8641 atagaacaaa actttgtact gaactgtacc tgccccatcg ctgactgtcc tgcccagccc
     8701 actggagcct tcatccgtaa cattgtctcc tccccggagg tcatctccaa gtatgagaag
     8761 gcccttctgc gaggctatgt ggaaagctgc tccaacctga cgtggtgcac caaccctcag
     8821 ggctgtgacc gcatcctgtg ccgccagggc ctgggcagtg ggaccacctg ctccaagtgc
     8881 ggctgggcct cttgcttcag ctgtagtttt cccgaggcac actaccctgc cagctgtggc
     8941 cacatgtcgc agtgggtgga tgatggtggc tactatgacg gcatgagcgt ggaggcccag
     9001 agcaagcact tggccaagct catctccaaa cgctgcccta gctgtcaggc gccgatcgag
     9061 aagaacgagg ggtgtctgca catgacctgt gcaagatgta accatgggtt ctgctggcgc
     9121 tgcctcaagt cctggaagcc aagccacaag gactactaca actgctctgc catggtaagc
     9181 aaagcagctc gccaggagaa gcggtttcag gactataacg agaggtgtac cttccatcat
     9241 caggcccggg agtttgctgt caacctgcgg aaccaggcct ctgccatcca agaggtgcct
     9301 ccacccaaat ccttcacctt cctgcaagat gcctgccggg ccctcgagca ggctcgaaag
     9361 gtgctggcct atgcctgtgt ctacagcttc tacagccagg acacagagta catggatgtg
     9421 gtagaacagc agacggagaa cctggagctg catacgaacg ccctgcagat cctgctcgag
     9481 gagaccctgc tgcgctgccg ggacctggcc tcctccctgc gcttcctgcg ggccgactgc
     9541 ctcagcacgg gcacagagct gctccggagg atccaggaga ggctgctggc catcctacag
     9601 cactccaccc aggacttccg ggttgggctg cagagtccct cagtagagac ccgggaggtg
     9661 aaagggtcca acgtgcccag tgatcaaccc cagggctcct cagggctgga agtggaagac
     9721 gaagaggagg aagaagagga ggaagaggag gaggaggagg aggaggaaga ggatgtgcct
     9781 gagtggcaac atgaatttga tgaggaacta gacaatgata gcttctccta cgacgaggag
     9841 tctgagaacc tggaccgaga gaccttcttc tttggtgatg aggatgaaga tgatgagtcc
     9901 tatgacggat ccgactacaa agaccatgac ggtgattata aagatcatga catcgactac
     9961 aaggatgacg atgacaagta gcggccgcga aggatctgcg atcgctccgg tgcccgtcag
    10021 tgggcagagc gcacatcgcc cacagtcccc gagaagttgg ggggaggggt cggcaattga
    10081 acgggtgcct agagaaggtg gcgcggggta aactgggaaa gtgatgtcgt gtactggctc
    10141 cgcctttttc ccgagggtgg gggagaaccg tatataagtg cagtagtcgc cgtgaacgtt
    10201 ctttttcgca acgggtttgc cgccagaaca cagctgaagc ttcgaggggc tcgcatctct
    10261 ccttcacgcg cccgccgccc tacctgaggc cgccatccac gccggttgag tcgcgttctg
    10321 ccgcctcccg cctgtggtgc ctcctgaact gcgtccgccg tctaggtaag tttaaagctc
    10381 aggtcgagac cgggcctttg tccggcgctc ccttggagcc tacctagact cagccggctc
    10441 tccacgcttt gcctgaccct gcttgctcaa ctctacgtct ttgtttcgtt ttctgttctg
    10501 cgccgttaca gatccaagct gtgaccggcg cctacgctag acgccaccat ggagagcgac
    10561 gagagcggcc tgcccgccat ggagatcgag tgccgcatca ccggcaccct gaacggcgtg
    10621 gagttcgagc tggtgggcgg cggagagggc acccccaagc agggccgcat gaccaacaag
    10681 atgaagagca ccaaaggcgc cctgaccttc agcccctacc tgctgagcca cgtgatgggc
    10741 tacggcttct accacttcgg cacctacccc agcggctacg agaacccctt cctgcacgcc
    10801 atcaacaacg gcggctacac caacacccgc atcgagaagt acgaggacgg cggcgtgctg
    10861 cacgtgagct tcagctaccg ctacgaggcc ggccgcgtga tcggcgactt caaggtggtg
    10921 ggcaccggct tccccgagga cagcgtgatc ttcaccgaca agatcatccg cagcaacgcc
    10981 accgtggagc acctgcaccc catgggcgat aacgtgctgg tgggcagctt cgcccgcacc
    11041 ttcagcctgc gcgacggcgg ctactacagc ttcgtggtgg acagccacat gcacttcaag
    11101 agcgccatcc accccagcat cctgcagaac gggggcccca tgttcgcctt ccgccgcgtg
    11161 gaggagctgc acagcaacac cgagctgggc atcgtggagt accagcacgc cttcaagacc
    11221 cccatcgcct tcgccagatc ccgcgctcag tcgtccaatt ctgccgtgga cggcaccgcc
    11281 ggacccggct ccaccggatc tcgcgagggc agaggaagtc ttctaacatg cggtgacgtg
    11341 gaggagaatc ccggccctat gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac
    11401 gacgtcccca gggccgtacg caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc
    11461 cacaccgtcg atccggaccg ccacatcgag cgggtcaccg agctgcaaga actcttcctc
    11521 acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg
    11581 gtctggacca cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc
    11641 atggccgagt tgagcggttc ccggctggcc gcgcagcaac agatggaagg cctcctggcg
    11701 ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac
    11761 cagggcaagg gtctgggcag cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc
    11821 ggggtgcccg ccttcctgga gacctccgcg ccccgcaacc tccccttcta cgagcggctc
    11881 ggcttcaccg tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc
    11941 cgcaagcccg gtgcctgaaa tcaacctctg gattacaaaa tttgtgaaag attgactggt
    12001 attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat
    12061 catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg
    12121 tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt
    12181 gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact
    12241 ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc
    12301 tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaaatcatcg
    12361 tcctttcctt ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc
    12421 tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg
    12481 cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc
    12541 tccccgcctg gtacctttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt
    12601 tttaaaagaa aaggggggac tggaagggct aattcactcc caacgaagat aagatctgct
    12661 ttttgcttgt actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta
    12721 actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg
    12781 tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg
    12841 gaaaatctct agcagtagta gttcatgtca tcttattatt cagtatttat aacttgcaaa
    12901 gaaatgaata tcagagagtg agaggaactt gtttattgca gcttataatg gttacaaata
    12961 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
    13021 tttgtccaaa ctcatcaatg tatcttatca tgtctggctc tagctatccc gcccctaact
    13081 ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta
    13141 atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag
    13201 tgaggaggct tttttggagg cctagacttt tgcagagacc aaattcgtaa tcatgtcata
    13261 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag
    13321 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg
    13381 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca
    13441 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc
    13501 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
    13561 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
    13621 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
    13681 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
    13741 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
    13801 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
    13861 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
    13921 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
    13981 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
    14041 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac
    14101 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
    14161 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
    14221 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
    14281 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
    14341 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
    14401 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
    14461 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
    14521 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
    14581 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
    14641 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
    14701 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt
    14761 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
    14821 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
    14881 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
    14941 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
    15001 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
    15061 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
    15121 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
    15181 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
    15241 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
    15301 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
    15361 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac
    15421 cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtctcgc
    15481 gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga cggtcacagc
    15541 ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg
    15601 cgggtgtcgg ggctggctta actatgcggc atcagagcag attgtactga gagtgcacca
    15661 tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcgccattc
    15721 gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg
    15781 ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc
    15841 ccagtcacga cgttgtaaaa cgacggccag tgccaagctg
//
