LOCUS       V037666                 9709 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V037666
VERSION     V037666
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1920
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2010..2213
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2302..4539
                     /label="DDX31(NM_022779)"
                     /note="DDX31(NM_022779)"
                     /gene="DDX31"
     CDS             4546..4635
                     /codon_start=1
                     /product="3 tandem Myc epitope tags"
                     /transl_table=1
                     /translation="EQKLISEEDLEQKLISEEDLEQKLISEEDL"
                     /label="3xMyc"
     promoter        4679..4890
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             4903..5171
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     CDS             5198..5797
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    5804..6392
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             6466..6699
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    6771..6892
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      6911..7046
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(7084..7100)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    7108..7124
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(7132..7162)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    7177..7198
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(7486..8074)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(8245..9105)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(9106..9210)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     9684..9700
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt
     1921 atcgatacta gtattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
     1981 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
     2041 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
     2101 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
     2161 tgacgcaaat gggcggtagg cgtgtacggt gggaggttta tataagcaga gctcgtttag
     2221 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagattct
     2281 agagctagcg aattcgccac catggcagcc gccgacggtt cgctcttcga caaccccagg
     2341 acgttctcca gacgtccccc agcccaggcg agtcggcaag caaaggctac gaaaagaaaa
     2401 taccaagcgt ccagtgaggc tcccccagcg aaacggagga acgaaacttc atttctccca
     2461 gccaagaaaa ctagtgttaa agaaactcag aggactttta aggggaacgc acaaaaaatg
     2521 ttttctccaa agaagcattc ggttagcaca agtgatagaa accaggagga gagacagtgc
     2581 attaagactt catcactgtt taaaaacaac cctgacattc cagaactcca cagacctgtg
     2641 gtaaagcagg tgcaagaaaa agtgtttact tcagctgctt ttcatgagct gggcctccac
     2701 ccacatttaa tttccacaat aaatacggtc ttaaaaatgt ctagtatgac cagtgttcag
     2761 aagcaaagta ttcctgtgtt gctggaaggc agagatgctc tcgtgagatc ccagacgggc
     2821 tcaggtaaaa ctcttgccta ttgcatccct gtggtccagt cccttcaagc aatggagtca
     2881 aaaatacagc gcagtgatgg cccctatgcc ctggtgctcg tgccaacgag agagctagct
     2941 ctacaaagct ttgacactgt ccagaaactg cttaagcctt tcacctggat tgtgcctgga
     3001 gtgttaatgg gaggagagaa gagaaaatca gaaaaggcca gactccgcaa aggaataaat
     3061 atccttatct caactcctgg acgcctggtg gatcatataa aatccacaaa gaacattcat
     3121 tttagtcggc tgcggtggtt ggtgtttgat gaagcagaca gaatcttgga tttgggtttt
     3181 gaaaaggaca tcacagtgat acttaatgct gtaaatgctg aatgccaaaa acgacagaat
     3241 gtcttgctat cagcgacact cacagaaggt gtaacgcggc tagctgatat cagtttgcat
     3301 gatccagtca gtatttctgt cctggacaag agccatgacc agttgaaccc aaaggacaaa
     3361 gcggtccagg aggtctgtcc tccaccagct ggcgacaagc tggacagctt tgcaatacca
     3421 gagagtctca agcagcatgt gactgtggtt cccagcaaac tgaggcttgt ctgcctagcg
     3481 gccttcatcc ttcagaaatg caagtttgag gaagaccaga agatggttgt ctttttctca
     3541 agttgcgagc tggtggagtt ccactacagc ctcttcctac agaccctgct gagcagctca
     3601 ggggcgccgg catcagggca gttgccatct gcctccatgc gattaaaatt cctacggctg
     3661 catggcggca tggagcagga ggaaagaaca gcagtgtttc aggaattttc acattccaga
     3721 agaggcgtcc ttctttgcac ggatgttgca gctcggggct tagatctccc tcaagtcacg
     3781 tggattgttc agtacaacgc tccatcttca cctgcagaat acatccaccg gattggaaga
     3841 accgcccgga ttggctgcca tgggagcagc ctgctcattt tggctccttc ggaggcagaa
     3901 tatgtcaact cgttggcttc tcacaaaatc aacgtttctg agattaagat ggaagatatt
     3961 ttgtgtgttc tgacaagaga tgattgtttt aaagggaaac gatggggagc ccagaaatcc
     4021 catgctgttg gcccccagga aatccgagag cgagccacag tcttgcagac ggtatttgaa
     4081 gattacgtgc actccagtga gaggagggtc tcctgggcaa agaaagctct gcagtccttc
     4141 atccaagcct acgccaccta ccccagggag ctgaagcaca tcttccacgt ccgatccctc
     4201 caccttgggc atgtggcgaa gagcttcgga ctaagagatg cccccaggaa tcttagtgcc
     4261 ttgactagaa agaagaggaa agcacacgtg aaaaggcctg accttcataa gaagacccag
     4321 agtaaacaca gcctcgctga aatcctacgt tcggaatact caagcggcat ggaggccgac
     4381 atcgccaagg tcaaaaagca aaacgcacct ggagagcctg gtggccggcc cctgcagcac
     4441 agtctgcagc cgacaccctg ctttggccgt gggaaaacat taaaatggag aaaaacccaa
     4501 aaaggtgtac agcgggacag caagacttcc cagaaagttg gatccgaaca aaaactaata
     4561 tcagaggaag atttggaaca gaaactaatc agtgaagaag atttagagca aaagttaatt
     4621 tccgaggagg acttatagcg gccgcgaagg atctgcgatc gctccggtgc ccgtcagtgg
     4681 gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacg
     4741 ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc
     4801 ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt
     4861 tttcgcaacg ggtttgccgc cagaacacag ctgaagcttc gaggggctcg catctctcct
     4921 tcacgcgccc gccgccctac ctgaggccgc catccacgcc ggttgagtcg cgttctgccg
     4981 cctcccgcct gtggtgcctc ctgaactgcg tccgccgtct aggtaagttt aaagctcagg
     5041 tcgagaccgg gcctttgtcc ggcgctccct tggagcctac ctagactcag ccggctctcc
     5101 acgctttgcc tgaccctgct tgctcaactc tacgtctttg tttcgttttc tgttctgcgc
     5161 cgttacagat ccaagctgtg accggcgcct acgctagatg accgagtaca agcccacggt
     5221 gcgcctcgcc acccgcgacg acgtcccccg ggccgtacgc accctcgccg ccgcgttcgc
     5281 cgactacccc gccacgcgcc acaccgtcga cccggaccgc cacatcgagc gggtcaccga
     5341 gctgcaagaa ctcttcctca cgcgcgtcgg gctcgacatc ggcaaggtgt gggtcgcgga
     5401 cgacggcgcc gcggtggcgg tctggaccac gccggagagc gtcgaagcgg gggcggtgtt
     5461 cgccgagatc ggcccgcgca tggccgagtt gagcggttcc cggctggccg cgcagcaaca
     5521 gatggaaggc ctcctggcgc cgcaccggcc caaggagccc gcgtggttcc tggccaccgt
     5581 cggcgtctcg cccgaccacc agggcaaggg tctgggcagc gccgtcgtgc tccccggagt
     5641 ggaggcggcc gagcgcgccg gggtgcccgc cttcctggag acctccgcgc cccgcaacct
     5701 ccccttctac gagcggctcg gcttcaccgt caccgccgac gtcgaggtgc ccgaaggacc
     5761 gcgcacctgg tgcatgaccc gcaagcccgg tgcctgagtc gacaatcaac ctctggatta
     5821 caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg
     5881 atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc
     5941 ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca
     6001 acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac
     6061 cacctgtcag ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact
     6121 catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc
     6181 cgtggtgttg tcggggaaat catcgtcctt tccttggctg ctcgcctgtg ttgccacctg
     6241 gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc
     6301 ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac
     6361 gagtcggatc tccctttggg ccgcctcccc gcctggtacc tttaagacca atgacttaca
     6421 aggcagctgt agatcttagc cactttttaa aagaaaaggg gggactggaa gggctaattc
     6481 actcccaacg aaaataagat ctgctttttg cttgtactgg gtctctctgg ttagaccaga
     6541 tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct
     6601 tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat
     6661 ccctcagacc cttttagtca gtgtggaaaa tctctagcag tagtagttca tgtcatctta
     6721 ttattcagta tttataactt gcaaagaaat gaatatcaga gagtgagagg aacttgttta
     6781 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat
     6841 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct
     6901 ggctctagct atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg
     6961 actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa
     7021 gtagtgagga ggcttttttg gaggcctaga cttttgcaga gacggcccaa attcgtaatc
     7081 atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg
     7141 agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat
     7201 tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg
     7261 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct
     7321 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
     7381 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
     7441 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
     7501 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
     7561 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
     7621 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
     7681 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
     7741 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
     7801 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
     7861 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
     7921 tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
     7981 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
     8041 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
     8101 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
     8161 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
     8221 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc
     8281 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
     8341 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc
     8401 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
     8461 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
     8521 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg
     8581 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
     8641 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
     8701 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
     8761 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
     8821 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc
     8881 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
     8941 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
     9001 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
     9061 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca
     9121 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat
     9181 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt
     9241 ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca cgaggccctt
     9301 tcgtctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac
     9361 ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc
     9421 gggtgttggc gggtgtcggg gctggcttaa ctatgcggca tcagagcaga ttgtactgag
     9481 agtgcaccat atgcggtgtg aaataccgca cagatgcgta aggagaaaat accgcatcag
     9541 gcgccattcg ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc
     9601 gctattacgc cagctggcga aagggggatg tgctgcaagg cgattaagtt gggtaacgcc
     9661 agggttttcc cagtcacgac gttgtaaaac gacggccagt gccaagctg
//
