LOCUS V036891 8828 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V036891 VERSION V036891 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers LTR 234..414 /note="truncated 5' long terminal repeat (LTR) from HIV-1" /label="5' LTR (truncated)" misc_feature 458..583 /note="packaging signal of human immunodeficiency virus type 1" /label="HIV-1 Ψ" misc_feature 1076..1309 /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." /label="RRE" CDS 1493..1537 /codon_start=1 /note="recognized by the 2H10 single-chain llama nanobody" /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /transl_table=1 /translation="KNEQELLELDKWASL" /label="gp41 peptide" misc_feature 1804..1920 /note="central polypurine tract and central termination sequence of HIV-1" /label="cPPT/CTS" promoter 2010..2213 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" CDS 2302..3660 /label="DLST(NM_001933)" /note="DLST(NM_001933)" /gene="DLST" CDS 3667..3693 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /transl_table=1 /translation="YPYDVPDYA" /label="HA" CDS 3697..3723 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /transl_table=1 /translation="YPYDVPDYA" /label="HA" CDS 3727..3753 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /transl_table=1 /translation="YPYDVPDYA" /label="HA" promoter 3798..4009 /note="core promoter for human elongation factor EF-1α" /label="EF-1α core promoter" LTR 4022..4290 /note="truncated 5' long terminal repeat (LTR) from human T-cell leukemia virus (HTLV) type 1" /label="5' LTR (truncated)" CDS 4317..4916 /codon_start=1 /gene="pac from Streptomyces alboniger" /note="confers resistance to puromycin" /product="puromycin N-acetyltransferase" /transl_table=1 /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*" /label="PuroR" misc_feature 4923..5511 /note="woodchuck hepatitis virus posttranscriptional regulatory element" /label="WPRE" LTR 5585..5818 /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" /label="3' LTR (ΔU3)" polyA_signal 5890..6011 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" rep_origin 6030..6165 /note="SV40 origin of replication" /label="SV40 ori" primer_bind complement(6203..6219) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 6227..6243 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(6251..6281) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 6296..6317 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(6605..7193) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(7364..8224) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(8225..8329) /gene="bla" /label="AmpR promoter" primer_bind 8803..8819 /note="common sequencing primer, one of multiple similar variants" /label="M13 fwd" ORIGIN 1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt 1921 atcgatacta gtattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca 1981 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc 2041 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga 2101 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat 2161 tgacgcaaat gggcggtagg cgtgtacggt gggaggttta tataagcaga gctcgtttag 2221 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagattct 2281 agagctagcg aattcgccac catgctgtcc cgatcccgct gtgtgtctcg ggcgttcagc 2341 cgctcgctct ccgccttcca gaaggggaac tgccctctag ggagacgttc cctgcctggg 2401 gtctccttat gccagggacc aggttaccct aacagcagga aggttgtcat taacaacagt 2461 gtcttcagtg ttcgcttttt cagaactaca gctgtatgca aggatgactt ggttacagtc 2521 aaaaccccag cgtttgcaga atctgtcaca gagggagatg tcaggtggga gaaagctgtt 2581 ggagacacag ttgcagaaga tgaagtggtt tgtgagattg aaactgacaa gacatctgtg 2641 caggttccat caccagcaaa tggcgtgatt gaagctcttt tggtacctga tgggggaaaa 2701 gtcgaaggag gcactccact tttcacactc aggaaaactg gtgctgctcc tgctaaggcc 2761 aagccggctg aagctcctgc tgctgcagcc ccaaaagcag aacctacagc agcggcagtt 2821 cctccccctg cagcacccat acccactcag atgccaccgg tgccctcgcc ctcacagcct 2881 ccttctggca aacctgtgtc tgcagtaaaa cccactgttg ccccaccact agctgagcca 2941 ggagctggca aaggtctgcg ttcagaacat cgggagaaaa tgaacaggat gcggcagcgc 3001 attgctcagc gtctgaagga ggcccagaat acatgtgcaa tgctgacaac ttttaatgag 3061 attgacatga gtaacatcca ggagatgagg gctcggcaca aagaggcttt tttgaagaaa 3121 cataacctca aactaggctt catgtcggca tttgtgaagg cctcagcctt tgccttgcag 3181 gaacagcctg ttgtaaatgc agtgattgac gacacaacca aagaggtggt gtatagggat 3241 tatattgaca tcagtgttgc agtggccacc ccacggggtc tggtggttcc agtcatcagg 3301 aatgtggaag ctatgaattt tgcagatatt gaacggacca tcactgaact gggagagaag 3361 gcccgaaaga atgaacttgc cattgaagat atggatggcg gtaccttcac cattagcaat 3421 ggaggcgttt ttggctcgct ctttggaaca cccattatca acccccctca gtctgccatc 3481 ctggggatgc atggcatctt tgacaggcca gtggctatag gaggcaaggt agaggtgcgg 3541 cccatgatgt acgtggcact gacctatgat caccggctga ttgatggcag agaggctgtg 3601 actttcctcc gcaaaatcaa ggcagcggta gaggatccca gagtcctcct cctggatctt 3661 ggatcctacc catacgacgt acctgattat gcaacttatc cttatgatgt acctgattat 3721 gctagctatc cttatgatgt cccagactac gcataggcgg ccgcgaagga tctgcgatcg 3781 ctccggtgcc cgtcagtggg cagagcgcac atcgcccaca gtccccgaga agttgggggg 3841 aggggtcggc aattgaacgg gtgcctagag aaggtggcgc ggggtaaact gggaaagtga 3901 tgtcgtgtac tggctccgcc tttttcccga gggtggggga gaaccgtata taagtgcagt 3961 agtcgccgtg aacgttcttt ttcgcaacgg gtttgccgcc agaacacagc tgaagcttcg 4021 aggggctcgc atctctcctt cacgcgcccg ccgccctacc tgaggccgcc atccacgccg 4081 gttgagtcgc gttctgccgc ctcccgcctg tggtgcctcc tgaactgcgt ccgccgtcta 4141 ggtaagttta aagctcaggt cgagaccggg cctttgtccg gcgctccctt ggagcctacc 4201 tagactcagc cggctctcca cgctttgcct gaccctgctt gctcaactct acgtctttgt 4261 ttcgttttct gttctgcgcc gttacagatc caagctgtga ccggcgccta cgctagatga 4321 ccgagtacaa gcccacggtg cgcctcgcca cccgcgacga cgtcccccgg gccgtacgca 4381 ccctcgccgc cgcgttcgcc gactaccccg ccacgcgcca caccgtcgac ccggaccgcc 4441 acatcgagcg ggtcaccgag ctgcaagaac tcttcctcac gcgcgtcggg ctcgacatcg 4501 gcaaggtgtg ggtcgcggac gacggcgccg cggtggcggt ctggaccacg ccggagagcg 4561 tcgaagcggg ggcggtgttc gccgagatcg gcccgcgcat ggccgagttg agcggttccc 4621 ggctggccgc gcagcaacag atggaaggcc tcctggcgcc gcaccggccc aaggagcccg 4681 cgtggttcct ggccaccgtc ggcgtctcgc ccgaccacca gggcaagggt ctgggcagcg 4741 ccgtcgtgct ccccggagtg gaggcggccg agcgcgccgg ggtgcccgcc ttcctggaga 4801 cctccgcgcc ccgcaacctc cccttctacg agcggctcgg cttcaccgtc accgccgacg 4861 tcgaggtgcc cgaaggaccg cgcacctggt gcatgacccg caagcccggt gcctgagtcg 4921 acaatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg 4981 ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc 5041 gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt 5101 tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca 5161 ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc 5221 ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc 5281 tgttgggcac tgacaattcc gtggtgttgt cggggaaatc atcgtccttt ccttggctgc 5341 tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc 5401 tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc 5461 ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg cctggtacct 5521 ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagggg 5581 ggactggaag ggctaattca ctcccaacga aaataagatc tgctttttgc ttgtactggg 5641 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg 5701 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt 5761 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt 5821 agtagttcat gtcatcttat tattcagtat ttataacttg caaagaaatg aatatcagag 5881 agtgagagga acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca 5941 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc 6001 aatgtatctt atcatgtctg gctctagcta tcccgcccct aactccgccc agttccgccc 6061 attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg 6121 cctctgagct attccagaag tagtgaggag gcttttttgg aggcctagac ttttgcagag 6181 acggcccaaa ttcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca 6241 caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag 6301 tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt 6361 cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc 6421 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg 6481 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa 6541 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg 6601 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga 6661 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg 6721 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg 6781 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc 6841 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg 6901 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca 6961 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt 7021 ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag 7081 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg 7141 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc 7201 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt 7261 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt 7321 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca 7381 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg 7441 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac 7501 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg 7561 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc 7621 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta 7681 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac 7741 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc 7801 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac 7861 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact 7921 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa 7981 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt 8041 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca 8101 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa 8161 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac 8221 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg 8281 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc 8341 gaaaagtgcc acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata 8401 ggcgtatcac gaggcccttt cgtctcgcgc gtttcggtga tgacggtgaa aacctctgac 8461 acatgcagct cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag 8521 cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac tatgcggcat 8581 cagagcagat tgtactgaga gtgcaccata tgcggtgtga aataccgcac agatgcgtaa 8641 ggagaaaata ccgcatcagg cgccattcgc cattcaggct gcgcaactgt tgggaagggc 8701 gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt gctgcaaggc 8761 gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg acggccagtg 8821 ccaagctg //