LOCUS       V045056                13645 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V045056
VERSION     V045056
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2317..7671
                     /label="DMBT1(NM_004406)"
                     /note="DMBT1(NM_004406)"
                     /gene="DMBT1"
     CDS             7678..7743
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        7787..7998
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             8011..8279
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      8308..8317
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             8338..9000
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             9070..9123
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             9124..9723
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    9724..10312
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             10386..10619
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    10691..10812
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      10852..10987
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(11020..11036)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    11044..11060
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(11068..11098)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    11113..11134
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(11422..12010)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(12181..13041)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(13042..13146)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     13620..13636
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc ctgatacgaa ctcggaattc gccaccatgg ggatctccac agtcatcctt
     2341 gaaatgtgtc ttttatgggg acaagttcta tctacaggtg ggtggatccc aaggactaca
     2401 gactacgctt cactgattcc ctcggaggtg cccttggatc caactgtagc agaaggttct
     2461 ccatttccct cggagtcgac cctggagtca actgtagcag aaggttctcc gatttccttg
     2521 gagtcaaccc tggagtcaac cgtagcagaa ggttctctga ttccctcaga gtcaaccctg
     2581 gagtcaactg tagcagaagg atctgattct ggtttggccc tgaggctggt gaatggagat
     2641 ggcaggtgtc agggccgagt ggagatccta taccgaggct cctggggcac cgtgtgtgat
     2701 gacagctggg acaccaatga tgccaacgtg gtctgtaggc agctgggttg tggctgggcc
     2761 atgtcagctc caggaaatgc ctggtttggc cagggctcag gacccattgc cctggatgat
     2821 gtgcgctgct caggacacga atcctacctg tggagctgcc cccacaatgg ctggctctcc
     2881 cataactgtg gccatggtga agatgctggt gttatctgct cagctgccca gcctcagtca
     2941 acactcaggc cagaaagttg gcctgtcagg atatcaccac ctgtacccac agaaggatct
     3001 gaatccagtt tggccctgag gctggtgaat ggaggcgaca ggtgtcgagg ccgagtggag
     3061 gtcctatacc gaggctcctg gggcaccgtg tgtgatgact actgggacac caatgatgcc
     3121 aatgtggtct gcaggcagct gggctgtggc tgggccatgt cagccccagg aaatgcccag
     3181 tttggccagg gctcaggacc cattgtcctg gatgatgtgc gctgctcagg acatgagtcc
     3241 tacctgtgga gctgccccca caatggctgg ctcacccaca actgtggcca tagtgaagac
     3301 gctggtgtca tctgctcagc tccccagtcc cggccgacac ccagcccaga tacttggccg
     3361 acctcacatg catcaacagc aggacctgaa tccagtttgg ccctgaggct ggtgaatgga
     3421 ggtgacaggt gtcagggccg agtggaggtc ctataccgag gctcctgggg caccgtgtgt
     3481 gatgatagct gggacaccag tgacgccaat gtggtctgcc ggcagctggg ctgtggctgg
     3541 gccacgtcag ccccaggaaa tgcccggttt ggccagggtt caggacccat tgtcctggat
     3601 gacgtgcgct gctcaggcta tgagtcctac ctgtggagct gcccccacaa tggctggctc
     3661 tcccataact gtcagcacag tgaagacgct ggtgtcatct gctcagctgc ccactcctgg
     3721 tcgacgccca gtccagacac attgccgacc atcaccttgc ctgcatcgac agtaggatct
     3781 gaatccagtt tggccctgag gctggtgaat ggaggtgaca ggtgtcaggg ccgagtggag
     3841 gtcctatacc aaggctcctg gggcaccgtg tgcgatgaca gctgggacac caatgatgcc
     3901 aatgtcgtct gcaggcaact gggctgtggc tgggccatgt cagccccagg aaatgcccgg
     3961 tttggtcagg gctcaggacc cattgtcctg gatgatgtgc gctgctcagg acacgagtct
     4021 tacctgtgga gctgccccca caatggctgg ctctcccaca actgtggcca tagtgaagac
     4081 gctggtgtca tctgctcagc ttcccagtcc cggccaacac ctagtccaga cacttggcca
     4141 acctcacatg catcaacagc aggatctgaa tccagtttgg ccctgaggct ggtgaatgga
     4201 ggtgacaggt gtcagggccg agtggaggtc ctataccgag gctcctgggg caccgtgtgt
     4261 gatgactact gggacaccaa tgatgccaat gtggtttgca ggcagctggg ctgtggctgg
     4321 gccatgtcag ccccaggaaa tgcccggttt ggccagggtt caggacccat tgtcctggat
     4381 gatgtgcgct gctcaggaca tgagtcctat ctgtggagct gcccccacaa tggctggctc
     4441 tcccacaact gtggccatca tgaagacgct ggtgtcatct gctcagcttc ccagtcccag
     4501 ccgacaccca gcccagacac ttggccaacc tcacatgcat caacagcagg atctgaatcc
     4561 agtttggccc tgaggctggt gaatggaggt gacaggtgtc agggccgagt ggaggtccta
     4621 taccgaggct cctggggcac cgtgtgtgat gactactggg acaccaatga tgccaatgtg
     4681 gtttgcaggc agctgggctg tggctgggcc acgtcagccc caggaaatgc ccggtttggc
     4741 cagggttcag gacccattgt cctggatgat gtgcgctgct caggacatga gtcctatctg
     4801 tggagctgcc cccacaatgg ctggctctcc cacaactgtg gccatcatga agacgctggt
     4861 gtcatctgct cagcttccca gtcccagccg acacccagcc cagacacttg gccaacctct
     4921 cgtgcatcaa cagcaggatc tgaatccact ttggccctga gactggtgaa tggaggtgac
     4981 aggtgtcgag gccgagtgga ggtcctatac caaggctcct ggggcaccgt gtgtgatgac
     5041 tactgggaca ccaatgatgc caacgtggtc tgcaggcagc tgggctgtgg ctgggccatg
     5101 tcagccccag gaaatgccca gtttggccag ggctcaggac ccattgtcct ggatgatgtg
     5161 cgctgctcag gacacgagtc ttacctgtgg agctgccccc acaatggctg gctctcccac
     5221 aactgtggcc atcatgaaga tgctggtgtc atctgctcag ctgctcagtc ccagtcaacg
     5281 cccaggccag atacttggct gaccaccaac ttaccggcat tgacagtagg atctgaatcc
     5341 agtttggctc tgaggctggt gaatggaggt gacaggtgtc gaggccgagt ggaggtcctg
     5401 tatcgaggct cctggggaac cgtgtgtgat gacagctggg acaccaatga tgccaatgtg
     5461 gtctgcaggc agctgggctg tggctgggcc atgtcggccc caggaaatgc ccggtttggc
     5521 cagggctcag gacccattgt cctggatgat gtgcgctgct cagggaatga gtcctacctg
     5581 tggagctgcc cccacaaagg ctggctcacc cacaactgtg gccatcacga agacgctggt
     5641 gtcatctgct cagccaccca aataaattct actacgacag attggtggca tccaacaact
     5701 acaaccactg caagaccctc ttcaaattgt ggtggcttct tattctatgc cagtgggaca
     5761 ttctccagcc catcctaccc tgcatactac cccaacaatg ctaagtgtgt ttgggaaata
     5821 gaagtgaatt ctggttatcg cataaacctg ggcttcagta atctgaaatt ggaggcacac
     5881 cataactgca gttttgatta tgttgaaatc tttgatggat cattgaatag cagtctcctg
     5941 ctggggaaaa tctgtaatga taccaggcaa atatttacat cttcttacaa ccgaatgacc
     6001 attcactttc gaagtgacat cagtttccaa aacactggct ttttggcttg gtataactcc
     6061 ttcccaagcg atgccacctt gaggttggtc aatttaaatt catcctatgg tctatgtgcc
     6121 gggcgtgtag aaatttacca tggtggcacc tgggggacag tttgtgatga ctcctggacc
     6181 attcaggaag ctgaggtggt ctgcagacag ctagggtgtg gacgtgcagt ttcagccctt
     6241 ggaaatgcat attttggctc tggctctggc cccatcaccc tggacgatgt agagtgctca
     6301 gggacggaat ccactctctg gcagtgccgg aaccgaggct ggttctccca caactgtaat
     6361 catcgtgaag atgctggtgt catctgctca ggaaaccatc tatcgacacc tgctcctttt
     6421 ctcaacatca cccgtccaaa cacagattat tcctgcggag gcttcctatc ccaaccatca
     6481 ggggactttt ccagcccatt ctatcccggg aactatccaa acaatgccaa gtgtgtgtgg
     6541 gacattgagg tgcaaaacaa ctaccgtgtg actgtgatct tcagagatgt ccagcttgaa
     6601 ggtggctgca actatgatta tattgaagtt ttcgatggcc cctaccgcag ttcccctctc
     6661 attgctcgag tttgtgatgg ggccagaggc tccttcactt cttcctccaa cttcatgtcc
     6721 attcgcttca tcagtgacca cagcatcaca aggagagggt tccgggctga gtactactcc
     6781 agtccctcca atgacagcac caacctgctc tgtctgccaa atcacatgca agccagtgtg
     6841 agcaggagct atctccaatc cttgggcttt tctgccagtg accttgtcat ttccacctgg
     6901 aatggatact acgagtgtcg gccccagata acgccgaacc tggtgatatt cacaattccc
     6961 tactcaggct gcggcacctt caagcaggca gacaatgaca ccatcgacta ttccaacttc
     7021 ctcacagcag ctgtctcagg tggcatcatc aagaggagga cagacctccg tattcacgtc
     7081 agctgcagaa tgcttcagaa cacctgggtc gacaccatgt acattgctaa tgacaccatc
     7141 cacgttgcta ataacaccat ccaggtcgag gaagtccagt atggcaattt tgacgtgaac
     7201 atttcctttt atacttcctc atctttcttg tatcctgtga ccagccgccc ttactacgtg
     7261 gacctgaacc aggacttgta cgttcaggct gaaatcctcc attctgatgc tgtactgacc
     7321 ttgtttgtgg acacctgcgt ggcatcacca tactccaatg acttcacgtc tttgacttat
     7381 gatctaatcc ggagtggatg cgtgagggat gacacctacg gaccctactc ctcgccatct
     7441 cttcgcattg cccgcttccg gttcagggcc ttccacttcc tgaaccgctt cccctccgtg
     7501 tacctgcgtt gtaaaatggt ggtgtgcaga gcgtatgacc cctcttcccg ctgctaccga
     7561 ggctgtgtgt tgaggtcgaa gagggatgtg ggctcctacc aggaaaaggt ggacgtcgtc
     7621 ctgggtccca tccagctgca gaccccccca cgccgagaag aggagcctcg gggatccgac
     7681 tacaaagacc atgacggtga ttataaagat catgacatcg actacaagga tgacgatgac
     7741 aagtagcggc cgcgaaggat ctgcgatcgc tccggtgccc gtcagtgggc agagcgcaca
     7801 tcgcccacag tccccgagaa gttgggggga ggggtcggca attgaacggg tgcctagaga
     7861 aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact ggctccgcct ttttcccgag
     7921 ggtgggggag aaccgtatat aagtgcagta gtcgccgtga acgttctttt tcgcaacggg
     7981 tttgccgcca gaacacagct gaagcttcga ggggctcgca tctctccttc acgcgcccgc
     8041 cgccctacct gaggccgcca tccacgccgg ttgagtcgcg ttctgccgcc tcccgcctgt
     8101 ggtgcctcct gaactgcgtc cgccgtctag gtaagtttaa agctcaggtc gagaccgggc
     8161 ctttgtccgg cgctcccttg gagcctacct agactcagcc ggctctccac gctttgcctg
     8221 accctgcttg ctcaactcta cgtctttgtt tcgttttctg ttctgcgccg ttacagatcc
     8281 aagctgtgac cggcgcctac gctagacgcc accatggaga gcgacgagag cggcctgccc
     8341 gccatggaga tcgagtgccg catcaccggc accctgaacg gcgtggagtt cgagctggtg
     8401 ggcggcggag agggcacccc caagcagggc cgcatgacca acaagatgaa gagcaccaaa
     8461 ggcgccctga ccttcagccc ctacctgctg agccacgtga tgggctacgg cttctaccac
     8521 ttcggcacct accccagcgg ctacgagaac cccttcctgc acgccatcaa caacggcggc
     8581 tacaccaaca cccgcatcga gaagtacgag gacggcggcg tgctgcacgt gagcttcagc
     8641 taccgctacg aggccggccg cgtgatcggc gacttcaagg tggtgggcac cggcttcccc
     8701 gaggacagcg tgatcttcac cgacaagatc atccgcagca acgccaccgt ggagcacctg
     8761 caccccatgg gcgataacgt gctggtgggc agcttcgccc gcaccttcag cctgcgcgac
     8821 ggcggctact acagcttcgt ggtggacagc cacatgcact tcaagagcgc catccacccc
     8881 agcatcctgc agaacggggg ccccatgttc gccttccgcc gcgtggagga gctgcacagc
     8941 aacaccgagc tgggcatcgt ggagtaccag cacgccttca agacccccat cgccttcgcc
     9001 agatcccgcg ctcagtcgtc caattctgcc gtggacggca ccgccggacc cggctccacc
     9061 ggatctcgcg agggcagagg aagtcttcta acatgcggtg acgtggagga gaatcccggc
     9121 cctatgaccg agtacaagcc cacggtgcgc ctcgccaccc gcgacgacgt ccccagggcc
     9181 gtacgcaccc tcgccgccgc gttcgccgac taccccgcca cgcgccacac cgtcgatccg
     9241 gaccgccaca tcgagcgggt caccgagctg caagaactct tcctcacgcg cgtcgggctc
     9301 gacatcggca aggtgtgggt cgcggacgac ggcgccgcgg tggcggtctg gaccacgccg
     9361 gagagcgtcg aagcgggggc ggtgttcgcc gagatcggcc cgcgcatggc cgagttgagc
     9421 ggttcccggc tggccgcgca gcaacagatg gaaggcctcc tggcgccgca ccggcccaag
     9481 gagcccgcgt ggttcctggc caccgtcggc gtctcgcccg accaccaggg caagggtctg
     9541 ggcagcgccg tcgtgctccc cggagtggag gcggccgagc gcgccggggt gcccgccttc
     9601 ctggagacct ccgcgccccg caacctcccc ttctacgagc ggctcggctt caccgtcacc
     9661 gccgacgtcg aggtgcccga aggaccgcgc acctggtgca tgacccgcaa gcccggtgcc
     9721 tgaaatcaac ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt
     9781 gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc
     9841 cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag
     9901 ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc
     9961 actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc
    10021 cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg
    10081 ctgttgggca ctgacaattc cgtggtgttg tcggggaaat catcgtcctt tccttggctg
    10141 ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc
    10201 ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt
    10261 cttcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc gcctggtacc
    10321 tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaaggg
    10381 gggactggaa gggctaattc actcccaacg aagataagat ctgctttttg cttgtactgg
    10441 gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact
    10501 gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg
    10561 tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag
    10621 tagtagttca tgtcatctta ttattcagta tttataactt gcaaagaaat gaatatcaga
    10681 gagtgagagg aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac
    10741 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat
    10801 caatgtatct tatcatgtct ggctctagct atcccgcccc taactccgcc catcccgccc
    10861 ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat
    10921 gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt
    10981 ggaggcctag acttttgcag agaccaaatt cgtaatcatg tcatagctgt ttcctgtgtg
    11041 aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc
    11101 ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt
    11161 ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg
    11221 cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt
    11281 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
    11341 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
    11401 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
    11461 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
    11521 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
    11581 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
    11641 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
    11701 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
    11761 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
    11821 agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg
    11881 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
    11941 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
    12001 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
    12061 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
    12121 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag
    12181 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
    12241 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
    12301 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa
    12361 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
    12421 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
    12481 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
    12541 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
    12601 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
    12661 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
    12721 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
    12781 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
    12841 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
    12901 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
    12961 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
    13021 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
    13081 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
    13141 tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa gaaaccatta ttatcatgac
    13201 attaacctat aaaaataggc gtatcacgag gccctttcgt ctcgcgcgtt tcggtgatga
    13261 cggtgaaaac ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga
    13321 tgccgggagc agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggctg
    13381 gcttaactat gcggcatcag agcagattgt actgagagtg caccatatgc ggtgtgaaat
    13441 accgcacaga tgcgtaagga gaaaataccg catcaggcgc cattcgccat tcaggctgcg
    13501 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
    13561 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
    13621 taaaacgacg gccagtgcca agctg
//
