LOCUS       V044427                12961 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V044427
VERSION     V044427
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1920
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2010..2213
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2302..7791
                     /label="DOCK2(NM_004946)"
                     /note="DOCK2(NM_004946)"
                     /gene="DOCK2"
     CDS             7798..7887
                     /codon_start=1
                     /product="3 tandem Myc epitope tags"
                     /transl_table=1
                     /translation="EQKLISEEDLEQKLISEEDLEQKLISEEDL"
                     /label="3xMyc"
     promoter        7931..8142
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             8155..8423
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     CDS             8450..9049
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    9056..9644
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             9718..9951
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    10023..10144
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      10163..10298
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(10336..10352)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    10360..10376
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(10384..10414)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    10429..10450
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(10738..11326)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(11497..12357)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(12358..12462)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     12936..12952
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt
     1921 atcgatacta gtattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
     1981 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
     2041 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
     2101 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
     2161 tgacgcaaat gggcggtagg cgtgtacggt gggaggttta tataagcaga gctcgtttag
     2221 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagattct
     2281 agagctagcg aattcgccac catggccccc tggcgcaaag ctgacaagga gcggcacggc
     2341 gtggccatat acaacttcca aggcagcgga gccccccagc tctccctgca gatcggcgat
     2401 gtggtgcgaa tacaggagac gtgtggagac tggtataggg gatacctcat aaagcacaaa
     2461 atgttacagg gcatttttcc taagtcattt atccacatca aggaagtgac agttgagaaa
     2521 agaagaaata ctgagaacat cattcctgca gaaattcctc tggcacaaga agtgacaacg
     2581 acactttggg aatggggaag catctggaaa caactctatg tggccagcaa aaaggagcgt
     2641 tttctccagg tgcagtccat gatgtacgat ctgatggagt ggaggtccca gcttctctca
     2701 ggaaccttac ccaaggatga gctgaaggaa ctgaagcaga aagtcacgtc caaaattgac
     2761 tatggcaaca aaatccttga gcttgatttg attgtcagag atgaagacgg aaatatcttg
     2821 gaccctgata ataccagtgt catcagcttg ttccatgcac atgaggaagc aactgataaa
     2881 atcacagagc gtatcaaaga agaaatgtca aaagaccagc cagattatgc aatgtattcc
     2941 cggatctcct catcccccac ccatagcctc tatgtgtttg tgagaaactt tgtgtgcaga
     3001 attggggaag atgctgagct cttcatgtct ctctacgacc ccaacaagca aacggtcata
     3061 agtgagaact acctagtgcg atggggcagc cggggcttcc ctaaggagat tgagatgctc
     3121 aacaatctga aggtggtctt cacggatctt ggaaacaaag acctcaacag ggataaaatt
     3181 tacttgattt gtcaaatagt ccgggtcggc aagatggatc ttaaggatac tggtgcaaag
     3241 aagtgcacgc agggactgag gaggcccttt ggggtggcag ttatggatat aacagacatc
     3301 atcaagggga aagcagagag tgatgaagaa aagcagcact tcattccttt tcacccggtt
     3361 acagctgaga atgacttcct acacagcctg ctgggcaaag tcatagcctc caagggggac
     3421 agtggagggc aaggcctctg ggtgaccatg aagatgctgg tgggtgacat cattcagatt
     3481 cgcaaggact atccacacct ggtggacagg accaccgtgg tggccaggaa gctgggattc
     3541 ccagagatca tcatgccagg ggatgtcagg aacgacatct acattactct cttacaaggt
     3601 gactttgaca agtacaacaa gaccacacag aggaatgtgg aagtcatcat gtgtgtgtgc
     3661 gcggaggatg gcaaaacgct gcctaatgca atttgcgtgg gagcagggga caagcccatg
     3721 aatgagtatc gctccgttgt gtactatcaa gtcaaacagc cacgctggat ggaaacagtc
     3781 aaggtggctg tccctattga agacatgcag aggatccatc tgcgattcat gtttcgacat
     3841 cggtcatctc tggaatctaa agataaagga gaaaagaact ttgccatgtc ctatgtgaag
     3901 ctgatgaaag aagatgggac tactctacac gatggattcc atgacttagt tgtcctcaag
     3961 ggggacagca agaagatgga ggatgccagc gcatacctga cccttccttc ttatcgacac
     4021 catgtggaaa acaagggggc cacgctgagc aggagctcca gcagtgttgg ggggctttct
     4081 gtcagctccc gggatgtgtt ctccatttcc accctggtgt gctccacaaa gctcactcag
     4141 aatgtgggct tgctgggttt gctgaagtgg cgtatgaagc ctcaactgct acaggagaat
     4201 ttagaaaagt tgaagattgt ggatggagag gaagtggtga agtttctcca ggatactctg
     4261 gatgccctct tcaacatcat gatggagcat tctcaaagtg atgaatatga catcctcgtc
     4321 tttgatgcct tgatttacat aataggactc attgcagacc ggaaatttca gcatttcaac
     4381 accgttctgg aggcttacat ccaacagcat ttcagtgcga ccttggctta caagaaattg
     4441 atgacagtgc tgaagactta cttggatacc tccagcagag gggagcaatg tgagccaatc
     4501 ctaagaacgc tgaaggcttt ggaatatgtg ttcaagttca ttgttcggtc gaggacatta
     4561 ttttcacagc tttatgaagg caaagaacag atggagtttg aagaatccat gagacggctc
     4621 tttgaatcca tcaacaatct gatgaaaagt caatacaaaa ctaccatcct tttgcaggtg
     4681 gcggctttga aatacatccc atctgtcctg catgatgtag aaatggtctt tgatgcgaag
     4741 ttactcagcc aactcctgta tgagttctac acctgcatcc ctcctgtgaa actccagaag
     4801 cagaaagtac agtctatgaa tgagatagtc cagagcaacc tctttaaaaa gcaagaatgc
     4861 cgggacattc tgcttcctgt catcaccaaa gagctgaagg agctgctgga gcagaaggat
     4921 gacatgcaac accaggtcct ggagaggaag tactgcgttg aattgctcaa cagcatcttg
     4981 gaagtcctta gctaccagga tgcggccttc acctaccacc atatccagga gatcatggtc
     5041 cagctgctgc ggacagtgaa ccggacagtc atcaccatgg gccgggatca cattctgatt
     5101 agtcactttg tggcatgtat gacagccatc ttaaaccaga tgggtgacca gcactactcc
     5161 ttctacattg agaccttcca gaccagctct gaacttgtgg acttcttgat ggagaccttc
     5221 atcatgttca aggacctcat tggaaagaac gtgtaccctg gagactggat ggccatgagc
     5281 atggttcaaa acagggtctt cctgagagct atcaacaagt ttgcagaaac catgaaccag
     5341 aagttcctag aacacacgaa ctttgagttc cagctgtgga acaactattt tcatctggca
     5401 gtggctttta tcacccagga ttctctgcag ctggagcagt tctcacacgc caaatacaac
     5461 aaaatcctga ataagtatgg ggacatgaga cggctaattg gcttctccat ccgtgatatg
     5521 tggtacaagc ttggtcagaa caaaatctgc ttcatcccag gcatggtagg acctatatta
     5581 gagatgacac ttatccctga ggctgagctc cggaaagcca ccataccaat cttcttcgac
     5641 atgatgctgt gtgaatatca aagaagtggg gatttcaaaa agtttgaaaa cgaaatcatc
     5701 ctgaagctgg accacgaggt agaagggggc cgaggcgacg agcagtacat gcagctcctg
     5761 gagtcaatcc tgatggaatg tgctgcagag cacccaacca ttgccaagtc ggtggagaac
     5821 ttcgtgaacc tggtcaaagg cctcctggag aagctgctgg attaccgggg tgtgatgaca
     5881 gatgagagca aagacaaccg catgagctgc accgtgaacc tgctgaattt ctacaaagat
     5941 aacaacaggg aggagatgta cataaggtac ctgtacaaac tccgcgatct tcacctggac
     6001 tgtgacaatt acacagaggc tgcctacacg ctccttctcc acacctggct tctcaagtgg
     6061 tcggatgagc agtgtgcatc acaggtcatg cagacaggcc agcagcaccc ccagacacac
     6121 cggcagctga aggagacgct ctacgagacc atcataggct actttgacaa aggaaagatg
     6181 tgggaagagg ccataagtct gtgcaaggag ctggcggaac agtacgagat ggagatcttt
     6241 gactatgagc tgctcagcca gaacctgatc cagcaggcaa aattctatga aagcatcatg
     6301 aaaatcctca ggcccaaacc agactacttt gctgttggat actacggcca gggattcccc
     6361 tccttcctgc ggaacaaagt gttcatctac cgcgggaagg aatatgagcg aagagaagat
     6421 ttccagatgc agctgatgac ccagttcccc aatgcagaga agatgaacac cacctctgcc
     6481 ccgggagatg atgtgaagaa tgccccaggc cagtatatcc agtgcttcac tgtccagcct
     6541 gtcttggatg aacatcccag gttcaagaat aagccagtgc ctgaccagat tataaacttc
     6601 tacaaatcca actacgtgca aaggttccac tactcccggc ccgtgcgcag ggggaccgta
     6661 gacccagaga atgagtttgc ttccatgtgg attgagagaa cctccttcgt gactgcatac
     6721 aagctgccgg ggatcctgcg ctggtttgag gtggtgcaca tgtcgcagac cacaattagt
     6781 cctctggaga atgccataga aaccatgtcc acggccaatg agaagatcct gatgatgata
     6841 aaccagtacc agagtgatga gaccctcccc atcaacccac tctccatgct cctgaacggg
     6901 attgtggacc ctgctgtcat gggaggcttc gccaagtatg agaaggcctt cttcactgaa
     6961 gagtatgtca gggaccaccc tgaggaccag gacaagctga cccacctcaa ggacctgatt
     7021 gcatggcaga tccccttctt gggagctggg attaagatcc atgagaaaag ggtgtcagat
     7081 aacttgcgac ccttccatga ccggatggag gaatgtttca agaacctgaa aatgaaggtg
     7141 gagaaggagt acggtgtccg agagatgcct gactttgacg acaggagagt gggccgtccc
     7201 aggtctatgc tgcgctcata cagacagatg tccatcatct ctctggcttc catgaattct
     7261 gactgcagca cccccagcaa gcctacctca gagagctttg acctggaatt agcatcaccc
     7321 aagacgccga gagtggagca ggaggaaccg atctccccgg ggagcaccct gcctgaggtc
     7381 aagctgcgga ggtccaagaa gaggacaaag agaagcagcg tagtttttgc ggatgagaaa
     7441 gcagctgcag agtcggacct gaagcggctt tccaggaagc atgagttcat gagtgacacc
     7501 aacctctcgg agcatgcggc catccccctc aaggcgtctg tcctctctca aatgagcttt
     7561 gccagccagt ccatgcctac catcccagcc ctggcgctct cagtggcagg catccctggg
     7621 ttggatgagg ccaacacatc tccccgcctc agccagacct tcctccaact ctcagatggt
     7681 gacaagaaga cactcacacg gaagaaggtc aatcagttct tcaagacaat gctggccagc
     7741 aaatcggctg aagaaggcaa acagatccca gactcgctgt ccacggacct gggatccgaa
     7801 caaaaactaa tatcagagga agatttggaa cagaaactaa tcagtgaaga agatttagag
     7861 caaaagttaa tttccgagga ggacttatag cggccgcgaa ggatctgcga tcgctccggt
     7921 gcccgtcagt gggcagagcg cacatcgccc acagtccccg agaagttggg gggaggggtc
     7981 ggcaattgaa cgggtgccta gagaaggtgg cgcggggtaa actgggaaag tgatgtcgtg
     8041 tactggctcc gcctttttcc cgagggtggg ggagaaccgt atataagtgc agtagtcgcc
     8101 gtgaacgttc tttttcgcaa cgggtttgcc gccagaacac agctgaagct tcgaggggct
     8161 cgcatctctc cttcacgcgc ccgccgccct acctgaggcc gccatccacg ccggttgagt
     8221 cgcgttctgc cgcctcccgc ctgtggtgcc tcctgaactg cgtccgccgt ctaggtaagt
     8281 ttaaagctca ggtcgagacc gggcctttgt ccggcgctcc cttggagcct acctagactc
     8341 agccggctct ccacgctttg cctgaccctg cttgctcaac tctacgtctt tgtttcgttt
     8401 tctgttctgc gccgttacag atccaagctg tgaccggcgc ctacgctaga tgaccgagta
     8461 caagcccacg gtgcgcctcg ccacccgcga cgacgtcccc cgggccgtac gcaccctcgc
     8521 cgccgcgttc gccgactacc ccgccacgcg ccacaccgtc gacccggacc gccacatcga
     8581 gcgggtcacc gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt
     8641 gtgggtcgcg gacgacggcg ccgcggtggc ggtctggacc acgccggaga gcgtcgaagc
     8701 gggggcggtg ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc
     8761 cgcgcagcaa cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt
     8821 cctggccacc gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt
     8881 gctccccgga gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc
     8941 gccccgcaac ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt
     9001 gcccgaagga ccgcgcacct ggtgcatgac ccgcaagccc ggtgcctgag tcgacaatca
     9061 acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt
     9121 tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc
     9181 tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc
     9241 cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg
     9301 gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc
     9361 cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg
     9421 cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg
     9481 tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc
     9541 agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct
     9601 tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcctggta cctttaagac
     9661 caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg
     9721 aagggctaat tcactcccaa cgaaaataag atctgctttt tgcttgtact gggtctctct
     9781 ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc
     9841 ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg
     9901 gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc agtagtagtt
     9961 catgtcatct tattattcag tatttataac ttgcaaagaa atgaatatca gagagtgaga
    10021 ggaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca
    10081 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat
    10141 cttatcatgt ctggctctag ctatcccgcc cctaactccg cccagttccg cccattctcc
    10201 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga
    10261 gctattccag aagtagtgag gaggcttttt tggaggccta gacttttgca gagacggccc
    10321 aaattcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc
    10381 acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta
    10441 actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca
    10501 gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc
    10561 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
    10621 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
    10681 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
    10741 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
    10801 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
    10861 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
    10921 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
    10981 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
    11041 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
    11101 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
    11161 ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt
    11221 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
    11281 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
    11341 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
    11401 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
    11461 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc
    11521 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta
    11581 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga
    11641 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg
    11701 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc
    11761 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat
    11821 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag
    11881 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat
    11941 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa
    12001 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa
    12061 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga
    12121 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg
    12181 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc
    12241 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg
    12301 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact
    12361 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat
    12421 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt
    12481 gccacctgac gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat
    12541 cacgaggccc tttcgtctcg cgcgtttcgg tgatgacggt gaaaacctct gacacatgca
    12601 gctcccggag acggtcacag cttgtctgta agcggatgcc gggagcagac aagcccgtca
    12661 gggcgcgtca gcgggtgttg gcgggtgtcg gggctggctt aactatgcgg catcagagca
    12721 gattgtactg agagtgcacc atatgcggtg tgaaataccg cacagatgcg taaggagaaa
    12781 ataccgcatc aggcgccatt cgccattcag gctgcgcaac tgttgggaag ggcgatcggt
    12841 gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa ggcgattaag
    12901 ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca gtgccaagct
    12961 g
//
