LOCUS       V035746                11993 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V035746
VERSION     V035746
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1920
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2010..2213
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2302..6912
                     /label="DOT1L(NM_032482)"
                     /note="DOT1L(NM_032482)"
                     /gene="DOT1L"
     promoter        6963..7174
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             7187..7455
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     CDS             7482..8081
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    8088..8676
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             8750..8983
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    9055..9176
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      9195..9330
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(9368..9384)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    9392..9408
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(9416..9446)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    9461..9482
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(9770..10358)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(10529..11389)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(11390..11494)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     11968..11984
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt
     1921 atcgatacta gtattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
     1981 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
     2041 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
     2101 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
     2161 tgacgcaaat gggcggtagg cgtgtacggt gggaggttta tataagcaga gctcgtttag
     2221 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagattct
     2281 agagctagcg aattcgccac catgggggag aagctggagc tgagactgaa gtcgcccgtg
     2341 ggggctgagc ccgccgtcta cccgtggccg ctgccggtct acgataaaca tcacgatgct
     2401 gctcatgaaa tcatcgagac catccgatgg gtctgtgaag aaatcccgga tctcaagctc
     2461 gctatggaga attacgtttt aattgactat gacaccaaaa gcttcgagag catgcagagg
     2521 ctctgcgaca agtacaaccg tgccatcgac agcatccacc agctgtggaa gggcaccacg
     2581 cagcccatga agctgaacac gcggccgtcc actggactcc tgcgccatat cctgcagcag
     2641 gtctacaacc actcggtgac cgaccccgag aagctcaaca actacgagcc cttctccccc
     2701 gaggtgtacg gggagacctc cttcgacctg gtggcccaga tgattgatga gatcaagatg
     2761 accgacgacg acctgtttgt ggacttgggg agcggtgtgg gccaggtcgt gctccaggtt
     2821 gctgctgcca ccaactgcaa acatcactat ggcgtcgaga aagcagacat cccggccaag
     2881 tatgcggaga ccatggaccg cgagttcagg aagtggatga aatggtatgg aaaaaagcat
     2941 gcagaataca cattggagag aggcgatttc ctctcagaag agtggaggga gcgaatcgcc
     3001 aacacgagtg ttatatttgt gaataatttt gcctttggtc ctgaggtgga tcaccagctg
     3061 aaggagcggt ttgcaaacat gaaggaaggt ggcagaatcg tgtcctcgaa accctttgca
     3121 cctctgaact tcagaataaa cagtagaaac ttgagtgaca tcggcaccat catgcgcgtg
     3181 gtggagctct cgcccctgaa gggctcggtg tcgtggacgg ggaagccagt ctcctactac
     3241 ctgcacacta tcgaccgcac catacttgaa aactattttt ctagtctgaa aaacccaaaa
     3301 ctcagggagg aacaggaggc agcccggcgc cgccagcagc gcgagagcaa gagcaacgcg
     3361 gccacgccca ctaagggccc agagggcaag gtggccggcc ccgccgacgc ccccatggac
     3421 tctggtgctg aggaagagaa ggcgggagca gccaccgtga agaagccgtc tccctccaaa
     3481 gcccgcaaga agaagctaaa caagaagggg aggaagatgg ctggccgcaa gcgcgggcgc
     3541 cccaagaaga tgaacactgc gaaccccgag cggaagccca agaagaacca aactgcactg
     3601 gatgccctgc acgctcagac cgtgtctcag acggcggcct cctcacccca ggatgcctac
     3661 agatcccctc acagcccgtt ctaccagcta cctccgagcg tgcagcggca ctcccccaac
     3721 ccgctgctgg tggcgcccac cccgcccgcg ctgcagaagc ttctagagtc cttcaagatc
     3781 cagtacctgc agttcctggc atacacaaag accccccagt acaaggccag cctgcaggag
     3841 ctgctgggcc aggagaagga gaagaacgcc cagctcctgg gtgcggctca gcagctcctc
     3901 agccactgcc aggcccagaa ggaggagatc aggaggctgt ttcagcaaaa attggatgag
     3961 ctgggtgtga aggcgctgac ctacaacgac ctgattcaag cgcagaagga gatctccgcc
     4021 cataaccagc agctgcggga gcagtcggag cagctggagc aggacaaccg cgcgctccgc
     4081 ggccagagct tgcagctgct caaggctcgc tgcgaggagc tgcagctgga ctgggccacg
     4141 ctgtcgctgg agaagctgtt gaaggagaag caggccctga agagccagat ctcggagaag
     4201 cagaggcact gcctggagct gcagatcagc attgtggagc tagagaagag ccagcggcag
     4261 caggagctcc tgcagctcaa gtcctgtgtg ccgcctgacg acgccctgtc cctgcacctg
     4321 cgtgggaagg gcgccctggg ccgcgagctg gagcctgacg ccagccggct gcacctggag
     4381 ctggactgca ccaagttctc gctgcctcac ttgagcagca tgagcccgga gctctccatg
     4441 aacggccagg ctgctggcta tgagctctgc ggtgtgctga gccggccttc gtcgaagcag
     4501 aacacgcccc agtacctggc ctcacccctg gaccaggagg tggtgccctg tacccctagc
     4561 cacgtcggcc ggccgcgcct ggagaagctg tctggcctag ccgcacccga ctacactagg
     4621 ctgtccccgg ccaagattgt gctgaggcgg cacctgagcc aggaccacac ggtgcccggc
     4681 aggccggctg ccagtgagct gcattcgaga gctgagcaca ccaaggagaa cggccttccc
     4741 taccagagcc ccagcgtgcc tggcagcatg aagctgagcc ctcaggaccc gcggcccctg
     4801 tcccctgggg ccttgcagct tgctggagag aagagcagtg agaagggcct gagagagcgc
     4861 gcctacggca gcagcgggga gctcatcacc agcctgccca tcagcatccc gctcagcacc
     4921 gtgcagccca acaagctccc ggtcagcatt cccctggcca gcgtggtgct gcccagccgc
     4981 gccgagaggg cgaggagcac ccccagtccc gtgctgcagc cccgtgaccc ctcgtccaca
     5041 cttgaaaagc agattggtgc taatgcccac ggtgctggga gcagaagcct tgccctggcc
     5101 cccgcaggct tctcctacgc tggctcggtg gccatcagcg gggccttggc gggcagcccg
     5161 gcctctctca cacctggagc cgagccggcc accttggatg agtcctccag ctctgggagc
     5221 ctttttgcca ccgtggggtc ccgcagctcc acgccacagc accccctgct gctggcacag
     5281 ccccggaact cgcttcctgc ctctcccgcc caccagctct cctccagtcc ccggcttggt
     5341 ggggccgccc agggcccgtt gcccgaggcc agcaagggag acctgccctc cgattccggc
     5401 ttctcagatc ctgagagtga agccaagagg aggattgtgt tcaccatcac cactggtgcg
     5461 ggcagtgcca agcagtcgcc ctccagcaag cacagccccc tgaccgccag cgcccgtggg
     5521 gactgtgtgc cgagccacgg gcaggacagt cgcaggcgcg gccggcggaa gcgagcatct
     5581 gcggggacgc ccagcttgag cgcaggcgtg tcccccaagc gccgagccct gccgtccgtc
     5641 gctggccttt tcacacagcc ttcggggtct cccctcaacc tcaactccat ggtcagtaac
     5701 atcaaccagc ccctggagat tacagccatc tcgtccccgg agacctccct gaagagctcc
     5761 cctgtgccct accaggacca cgaccagccc cccgtgctca agaaggagcg gcctctgagc
     5821 cagaccaatg gggcacacta ctccccactc acctcagacg aggagccagg ctctgaggac
     5881 gagcccagca gtgctcgaat tgagagaaaa attgcaacaa tctccttaga aagcaaatct
     5941 cccccgaaaa ccttggaaaa tggtggtggc ttggcgggaa ggaagcccgc gcccgccggc
     6001 gagccagtca atagcagcaa gtggaagtcc accttctcgc ccatctccga catcggcctg
     6061 gccaagtcgg cggacagccc gctgcaggcc agctccgccc tcagccagaa ctccctgttc
     6121 acgttccggc ccgccctgga ggagccctct gccgatgcca agctggccgc tcaccccagg
     6181 aaaggctttc ccggctccct gtcgggggct gacggactca gcccgggcac caaccctgcc
     6241 aacggctgca ccttcggcgg gggcctggcc gcggacctga gtttacacag cttcagtgat
     6301 ggtgcttctc ttccccacaa gggccccgag gcggccggcc tgagctcccc gctgagcttc
     6361 ccctcgcagc gcggcaagga gggctcggac gccaaccctt tcctgagcaa gaggcagctg
     6421 gacggcctgg ctgggctgaa gggcgagggc agccgcggca aggaggcagg ggagggcggc
     6481 ctaccgctgt gcgggcccac ggacaagacc ccactgctga gcggcaaggc cgccaaggcc
     6541 cgggaccgcg aggtcgacct caagaatggc cacaacctct tcatctctgc ggcggccgtg
     6601 cctcccggaa gcctcctcag cggccccggc ctggccccgg cggcgtcctc cgcaggcggc
     6661 gcggcgtcct ccgcccagac gcaccggtcc ttcctgggcc ccttcccgcc gggaccgcag
     6721 ttcgcgctcg gccccatgtc cctgcaggcc aacctcggct ccgtggccgg ctcctccgtg
     6781 ctgcagtcgc tgttcagctc tgtgccggcc gccgcaggcc tggtgcacgt gtcgtccgct
     6841 gccaccagac tgaccaactc gcacgccatg ggcagctttt ccggggtggc aggcggcaca
     6901 gttggaggta actagggatc cgcggccgcg aaggatctgc gatcgctccg gtgcccgtca
     6961 gtgggcagag cgcacatcgc ccacagtccc cgagaagttg gggggagggg tcggcaattg
     7021 aacgggtgcc tagagaaggt ggcgcggggt aaactgggaa agtgatgtcg tgtactggct
     7081 ccgccttttt cccgagggtg ggggagaacc gtatataagt gcagtagtcg ccgtgaacgt
     7141 tctttttcgc aacgggtttg ccgccagaac acagctgaag cttcgagggg ctcgcatctc
     7201 tccttcacgc gcccgccgcc ctacctgagg ccgccatcca cgccggttga gtcgcgttct
     7261 gccgcctccc gcctgtggtg cctcctgaac tgcgtccgcc gtctaggtaa gtttaaagct
     7321 caggtcgaga ccgggccttt gtccggcgct cccttggagc ctacctagac tcagccggct
     7381 ctccacgctt tgcctgaccc tgcttgctca actctacgtc tttgtttcgt tttctgttct
     7441 gcgccgttac agatccaagc tgtgaccggc gcctacgcta gatgaccgag tacaagccca
     7501 cggtgcgcct cgccacccgc gacgacgtcc cccgggccgt acgcaccctc gccgccgcgt
     7561 tcgccgacta ccccgccacg cgccacaccg tcgacccgga ccgccacatc gagcgggtca
     7621 ccgagctgca agaactcttc ctcacgcgcg tcgggctcga catcggcaag gtgtgggtcg
     7681 cggacgacgg cgccgcggtg gcggtctgga ccacgccgga gagcgtcgaa gcgggggcgg
     7741 tgttcgccga gatcggcccg cgcatggccg agttgagcgg ttcccggctg gccgcgcagc
     7801 aacagatgga aggcctcctg gcgccgcacc ggcccaagga gcccgcgtgg ttcctggcca
     7861 ccgtcggcgt ctcgcccgac caccagggca agggtctggg cagcgccgtc gtgctccccg
     7921 gagtggaggc ggccgagcgc gccggggtgc ccgccttcct ggagacctcc gcgccccgca
     7981 acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc cgacgtcgag gtgcccgaag
     8041 gaccgcgcac ctggtgcatg acccgcaagc ccggtgcctg agtcgacaat caacctctgg
     8101 attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat
     8161 gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt
     8221 tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca
     8281 ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg
     8341 ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg
     8401 aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca
     8461 attccgtggt gttgtcgggg aaatcatcgt cctttccttg gctgctcgcc tgtgttgcca
     8521 cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc
     8581 ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc
     8641 agacgagtcg gatctccctt tgggccgcct ccccgcctgg tacctttaag accaatgact
     8701 tacaaggcag ctgtagatct tagccacttt ttaaaagaaa aggggggact ggaagggcta
     8761 attcactccc aacgaaaata agatctgctt tttgcttgta ctgggtctct ctggttagac
     8821 cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa
     8881 agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag
     8941 agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtagtag ttcatgtcat
     9001 cttattattc agtatttata acttgcaaag aaatgaatat cagagagtga gaggaacttg
     9061 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa
     9121 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat
     9181 gtctggctct agctatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg
     9241 gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc
     9301 agaagtagtg aggaggcttt tttggaggcc tagacttttg cagagacggc ccaaattcgt
     9361 aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca
     9421 tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat
     9481 taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt
     9541 aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct
     9601 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
     9661 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
     9721 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
     9781 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
     9841 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
     9901 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
     9961 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
    10021 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
    10081 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
    10141 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
    10201 acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
    10261 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
    10321 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
    10381 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
    10441 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
    10501 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct
    10561 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta
    10621 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct
    10681 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg
    10741 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa
    10801 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt
    10861 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta
    10921 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca
    10981 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta
    11041 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct
    11101 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg
    11161 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac
    11221 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact
    11281 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa
    11341 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt
    11401 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat
    11461 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg
    11521 acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc
    11581 cctttcgtct cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg
    11641 agacggtcac agcttgtctg taagcggatg ccgggagcag acaagcccgt cagggcgcgt
    11701 cagcgggtgt tggcgggtgt cggggctggc ttaactatgc ggcatcagag cagattgtac
    11761 tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga aaataccgca
    11821 tcaggcgcca ttcgccattc aggctgcgca actgttggga agggcgatcg gtgcgggcct
    11881 cttcgctatt acgccagctg gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa
    11941 cgccagggtt ttcccagtca cgacgttgta aaacgacggc cagtgccaag ctg
//
