LOCUS       V022987                10919 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V022987
VERSION     V022987
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2317..4962
                     /label="DPP8(NM_130434)"
                     /note="DPP8(NM_130434)"
                     /gene="DPP8"
     CDS             4969..5034
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        5078..5289
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             5302..5570
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      5599..5608
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             5629..6291
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             6361..6414
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             6415..7014
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    7015..7602
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7676..7909
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    7981..8102
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      8121..8256
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(8294..8310)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8318..8334
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8342..8372)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8387..8408
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8696..9284)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9455..10315)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10316..10420)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     10894..10910
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc ctgatacgaa ctcggaattc gccaccatgg cagcagcaat ggaaacagaa
     2341 cagctgggtg ttgagatatt tgaaactgcg gactgtgagg agaatattga atcacaggat
     2401 cggcctaaat tggagccttt ttatgttgag cggtattcct ggagtcagct taaaaagctg
     2461 cttgccgata ccagaaaata tcatggctac atgatggcta aggcaccaca tgatttcatg
     2521 tttgtgaaga ggaatgatcc agatggacct cattcagaca gaatctatta ccttgccatg
     2581 tctggtgaga acagagaaaa tacactgttt tattctgaaa ttcccaaaac tatcaataga
     2641 gcagcagtct taatgctctc ttggaagcct cttttggatc tttttcaggc aacactggac
     2701 tatggaatgt attctcgaga agaagaacta ttaagagaaa gaaaacgcat tggaacagtc
     2761 ggaattgctt cttacgatta tcaccaagga agtggaacat ttctgtttca agccggtagt
     2821 ggaatttatc acgtaaaaga tggagggcca caaggattta cgcaacaacc tttaaggccc
     2881 aatctagtgg aaactagttg tcccaacata cggatggatc caaaattatg ccctgctgat
     2941 ccagactgga ttgcttttat acatagcaac gatatttgga tatctaacat cgtaaccaga
     3001 gaagaaagga gactcactta tgtgcacaat gagctagcca acatggaaga agatgccaga
     3061 tcagctggag tcgctacctt tgttctccaa gaagaatttg atagatattc tggctattgg
     3121 tggtgtccaa aagctgaaac aactcccagt ggtggtaaaa ttcttagaat tctatatgaa
     3181 gaaaatgatg aatctgaggt ggaaattatt catgttacat cccctatgtt ggaaacaagg
     3241 agggcagatt cattccgtta tcctaaaaca ggtacagcaa atcctaaagt cacttttaag
     3301 atgtcagaaa taatgattga tgctgaagga aggatcatag atgtcataga taaggaacta
     3361 attcaacctt ttgagattct atttgaagga gttgaatata ttgccagagc tggatggact
     3421 cctgagggaa aatatgcttg gtccatccta ctagatcgct cccagactcg cctacagata
     3481 gtgttgatct cacctgaatt atttatccca gtagaagatg atgttatgga aaggcagaga
     3541 ctcattgagt cagtgcctga ttctgtgacg ccactaatta tctatgaaga aacaacagac
     3601 atctggataa atatccatga catctttcat gtttttcccc aaagtcacga agaggaaatt
     3661 gagtttattt ttgcctctga atgcaaaaca ggtttccgtc atttatacaa aattacatct
     3721 attttaaagg aaagcaaata taaacgatcc agtggtgggc tgcctgctcc aagtgatttc
     3781 aagtgtccta tcaaagagga gatagcaatt accagtggtg aatgggaagt tcttggccgg
     3841 catggatcta atatccaagt tgatgaagtc agaaggctgg tatattttga aggcaccaaa
     3901 gactcccctt tagagcatca cctgtacgta gtcagttacg taaatcctgg agaggtgaca
     3961 aggctgactg accgtggcta ctcacattct tgctgcatca gtcagcactg tgacttcttt
     4021 ataagtaagt atagtaacca gaagaatcca cactgtgtgt ccctttacaa gctatcaagt
     4081 cctgaagatg acccaacttg caaaacaaag gaattttggg ccaccatttt ggattcagca
     4141 ggtcctcttc ctgactatac tcctccagaa attttctctt ttgaaagtac tactggattt
     4201 acattgtatg ggatgctcta caagcctcat gatctacagc ctggaaagaa atatcctact
     4261 gtgctgttca tatatggtgg tcctcaggtg cagttggtga ataatcggtt taaaggagtc
     4321 aagtatttcc gcttgaatac cctagcctct ctaggttatg tggttgtagt gatagacaac
     4381 aggggatcct gtcaccgagg gcttaaattt gaaggcgcct ttaaatataa aatgggtcaa
     4441 atagaaattg acgatcaggt ggaaggactc caatatctag cttctcgata tgatttcatt
     4501 gacttagatc gtgtgggcat ccacggctgg tcctatggag gatacctctc cctgatggca
     4561 ttaatgcaga ggtcagatat cttcagggtt gctattgctg gggccccagt cactctgtgg
     4621 atcttctatg atacaggata cacggaacgt tatatgggtc accctgacca gaatgaacag
     4681 ggctattact taggatctgt ggccatgcaa gcagaaaagt tcccctctga accaaatcgt
     4741 ttactgctct tacatggttt cctggatgag aatgtccatt ttgcacatac cagtatatta
     4801 ctgagttttt tagtgagggc tggaaagcca tatgatttac agatctatcc tcaggagaga
     4861 cacagcataa gagttcctga atcgggagaa cattatgaac tgcatctttt gcactacctt
     4921 caagaaaacc ttggatcacg tattgctgct ctaaaagtga taggatccga ctacaaagac
     4981 catgacggtg attataaaga tcatgacatc gactacaagg atgacgatga caagtagcgg
     5041 ccgcgaagga tctgcgatcg ctccggtgcc cgtcagtggg cagagcgcac atcgcccaca
     5101 gtccccgaga agttgggggg aggggtcggc aattgaacgg gtgcctagag aaggtggcgc
     5161 ggggtaaact gggaaagtga tgtcgtgtac tggctccgcc tttttcccga gggtggggga
     5221 gaaccgtata taagtgcagt agtcgccgtg aacgttcttt ttcgcaacgg gtttgccgcc
     5281 agaacacagc tgaagcttcg aggggctcgc atctctcctt cacgcgcccg ccgccctacc
     5341 tgaggccgcc atccacgccg gttgagtcgc gttctgccgc ctcccgcctg tggtgcctcc
     5401 tgaactgcgt ccgccgtcta ggtaagttta aagctcaggt cgagaccggg cctttgtccg
     5461 gcgctccctt ggagcctacc tagactcagc cggctctcca cgctttgcct gaccctgctt
     5521 gctcaactct acgtctttgt ttcgttttct gttctgcgcc gttacagatc caagctgtga
     5581 ccggcgccta cgctagacgc caccatggag agcgacgaga gcggcctgcc cgccatggag
     5641 atcgagtgcc gcatcaccgg caccctgaac ggcgtggagt tcgagctggt gggcggcgga
     5701 gagggcaccc ccaagcaggg ccgcatgacc aacaagatga agagcaccaa aggcgccctg
     5761 accttcagcc cctacctgct gagccacgtg atgggctacg gcttctacca cttcggcacc
     5821 taccccagcg gctacgagaa ccccttcctg cacgccatca acaacggcgg ctacaccaac
     5881 acccgcatcg agaagtacga ggacggcggc gtgctgcacg tgagcttcag ctaccgctac
     5941 gaggccggcc gcgtgatcgg cgacttcaag gtggtgggca ccggcttccc cgaggacagc
     6001 gtgatcttca ccgacaagat catccgcagc aacgccaccg tggagcacct gcaccccatg
     6061 ggcgataacg tgctggtggg cagcttcgcc cgcaccttca gcctgcgcga cggcggctac
     6121 tacagcttcg tggtggacag ccacatgcac ttcaagagcg ccatccaccc cagcatcctg
     6181 cagaacgggg gccccatgtt cgccttccgc cgcgtggagg agctgcacag caacaccgag
     6241 ctgggcatcg tggagtacca gcacgccttc aagaccccca tcgccttcgc cagatcccgc
     6301 gctcagtcgt ccaattctgc cgtggacggc accgccggac ccggctccac cggatctcgc
     6361 gagggcagag gaagtcttct aacatgcggt gacgtggagg agaatcccgg ccctatgacc
     6421 gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg tccccagggc cgtacgcacc
     6481 ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca ccgtcgatcc ggaccgccac
     6541 atcgagcggg tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc
     6601 aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc
     6661 gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg
     6721 ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg
     6781 tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc
     6841 gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt cctggagacc
     6901 tccgcgcccc gcaacctccc cttctacgag cggctcggct tcaccgtcac cgccgacgtc
     6961 gaggtgcccg aaggaccgcg cacctggtgc atgacccgca agcccggtgc ctgaaatcaa
     7021 cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt
     7081 acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct
     7141 ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc
     7201 gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg
     7261 ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc
     7321 acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc
     7381 actgacaatt ccgtggtgtt gtcggggaag ctgacgtcct ttccatggct gctcgcctgt
     7441 gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca
     7501 gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tctccgcctt
     7561 cgccctcaga cgagtcggat ctccctttgg ccgcctcccc gcctggtacc tttaagacca
     7621 atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaaggg gggactggaa
     7681 gggctaattc actcccaacg aaaataagat ctgctttttg cttgtactgg gtctctctgg
     7741 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct
     7801 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt
     7861 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tagtagttca
     7921 tgtcatctta ttattcagta tttataactt gcaaagaaat gaatatcaga gagtgagagg
     7981 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca
     8041 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct
     8101 tatcatgtct ggctctagct atcccgcccc taactccgcc cagttccgcc cattctccgc
     8161 cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc
     8221 tattccagaa gtagtgagga ggcttttttg gaggcctaga cttttgcaga gacggcccaa
     8281 attcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac
     8341 acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac
     8401 tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc
     8461 tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg
     8521 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc
     8581 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
     8641 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
     8701 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
     8761 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
     8821 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
     8881 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
     8941 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
     9001 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
     9061 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
     9121 acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
     9181 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
     9241 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
     9301 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
     9361 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
     9421 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac
     9481 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga
     9541 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc
     9601 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca
     9661 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta
     9721 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg
     9781 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc
     9841 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg
     9901 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt
     9961 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt
    10021 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata
    10081 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc
    10141 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac
    10201 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa
    10261 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct
    10321 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat
    10381 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc
    10441 cacctgacgt ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca
    10501 cgaggccctt tcgtctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc
    10561 tcccggagac ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg
    10621 gcgcgtcagc gggtgttggc gggtgtcggg gctggcttaa ctatgcggca tcagagcaga
    10681 ttgtactgag agtgcaccat atgcggtgtg aaataccgca cagatgcgta aggagaaaat
    10741 accgcatcag gcgccattcg ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc
    10801 gggcctcttc gctattacgc cagctggcga aagggggatg tgctgcaagg cgattaagtt
    10861 gggtaacgcc agggttttcc cagtcacgac gttgtaaaac gacggccagt gccaagctg
//
