LOCUS       V045873                10775 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V045873
VERSION     V045873
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2303..4612
                     /label="APP(NM_000484)"
                     /note="APP(NM_000484)"
                     /gene="APP"
     CDS             4619..4684
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        4728..4939
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             4952..5220
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      5249..5258
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             5279..5941
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             6011..6064
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             6071..6865
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MAEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     misc_feature    6872..7460
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7532..7765
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    7837..7958
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      7977..8112
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(8150..8166)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8174..8190
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8198..8228)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8243..8264
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8552..9140)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9311..10171)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10172..10276)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     10750..10766
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc gaattcgcca ccatgctgcc cggtttggca ctgctcctgc tggccgcctg
     2341 gacggctcgg gcgctggagg tacccactga tggtaatgct ggcctgctgg ctgaacccca
     2401 gattgccatg ttctgtggca gactgaacat gcacatgaat gtccagaatg ggaagtggga
     2461 ttcagatcca tcagggacca aaacctgcat tgataccaag gaaggcatcc tgcagtattg
     2521 ccaagaagtc taccctgaac tgcagatcac caatgtggta gaagccaacc aaccagtgac
     2581 catccagaac tggtgcaagc ggggccgcaa gcagtgcaag acccatcccc actttgtgat
     2641 tccctaccgc tgcttagttg gtgagtttgt aagtgatgcc cttctcgttc ctgacaagtg
     2701 caaattctta caccaggaga ggatggatgt ttgcgaaact catcttcact ggcacaccgt
     2761 cgccaaagag acatgcagtg agaagagtac caacttgcat gactacggca tgttgctgcc
     2821 ctgcggaatt gacaagttcc gaggggtaga gtttgtgtgt tgcccactgg ctgaagaaag
     2881 tgacaatgtg gattctgctg atgcggagga ggatgactcg gatgtctggt ggggcggagc
     2941 agacacagac tatgcagatg ggagtgaaga caaagtagta gaagtagcag aggaggaaga
     3001 agtggctgag gtggaagaag aagaagccga tgatgacgag gacgatgagg atggtgatga
     3061 ggtagaggaa gaggctgagg aaccctacga agaagccaca gagagaacca ccagcattgc
     3121 caccaccacc accaccacca cagagtctgt ggaagaggtg gttcgagagg tgtgctctga
     3181 acaagccgag acggggccgt gccgagcaat gatctcccgc tggtactttg atgtgactga
     3241 agggaagtgt gccccattct tttacggcgg atgtggcggc aaccggaaca actttgacac
     3301 agaagagtac tgcatggccg tgtgtggcag cgccatgtcc caaagtttac tcaagactac
     3361 ccaggaacct cttgcccgag atcctgttaa acttcctaca acagcagcca gtacccctga
     3421 tgccgttgac aagtatctcg agacacctgg ggatgagaat gaacatgccc atttccagaa
     3481 agccaaagag aggcttgagg ccaagcaccg agagagaatg tcccaggtca tgagagaatg
     3541 ggaagaggca gaacgtcaag caaagaactt gcctaaagct gataagaagg cagttatcca
     3601 gcatttccag gagaaagtgg aatctttgga acaggaagca gccaacgaga gacagcagct
     3661 ggtggagaca cacatggcca gagtggaagc catgctcaat gaccgccgcc gcctggccct
     3721 ggagaactac atcaccgctc tgcaggctgt tcctcctcgg cctcgtcacg tgttcaatat
     3781 gctaaagaag tatgtccgcg cagaacagaa ggacagacag cacaccctaa agcatttcga
     3841 gcatgtgcgc atggtggatc ccaagaaagc cgctcagatc cggtcccagg ttatgacaca
     3901 cctccgtgtg atttatgagc gcatgaatca gtctctctcc ctgctctaca acgtgcctgc
     3961 agtggccgag gagattcagg atgaagttga tgagctgctt cagaaagagc aaaactattc
     4021 agatgacgtc ttggccaaca tgattagtga accaaggatc agttacggaa acgatgctct
     4081 catgccatct ttgaccgaaa cgaaaaccac cgtggagctc cttcccgtga atggagagtt
     4141 cagcctggac gatctccagc cgtggcattc ttttggggct gactctgtgc cagccaacac
     4201 agaaaacgaa gttgagcctg ttgatgcccg ccctgctgcc gaccgaggac tgaccactcg
     4261 accaggttct gggttgacaa atatcaagac ggaggagatc tctgaagtga agatggatgc
     4321 agaattccga catgactcag gatatgaagt tcatcatcaa aaattggtgt tctttgcaga
     4381 agatgtgggt tcaaacaaag gtgcaatcat tggactcatg gtgggcggtg ttgtcatagc
     4441 gacagtgatc gtcatcacct tggtgatgct gaagaagaaa cagtacacat ccattcatca
     4501 tggtgtggtg gaggttgacg ccgctgtcac cccagaggag cgccacctgt ccaagatgca
     4561 gcagaacggc tacgaaaatc caacctacaa gttctttgag cagatgcaga acggatccga
     4621 ctacaaagac catgacggtg attataaaga tcatgacatc gactacaagg atgacgatga
     4681 caagtagcgg ccgcgaagga tctgcgatcg ctccggtgcc cgtcagtggg cagagcgcac
     4741 atcgcccaca gtccccgaga agttgggggg aggggtcggc aattgaacgg gtgcctagag
     4801 aaggtggcgc ggggtaaact gggaaagtga tgtcgtgtac tggctccgcc tttttcccga
     4861 gggtggggga gaaccgtata taagtgcagt agtcgccgtg aacgttcttt ttcgcaacgg
     4921 gtttgccgcc agaacacagc tgaagcttcg aggggctcgc atctctcctt cacgcgcccg
     4981 ccgccctacc tgaggccgcc atccacgccg gttgagtcgc gttctgccgc ctcccgcctg
     5041 tggtgcctcc tgaactgcgt ccgccgtcta ggtaagttta aagctcaggt cgagaccggg
     5101 cctttgtccg gcgctccctt ggagcctacc tagactcagc cggctctcca cgctttgcct
     5161 gaccctgctt gctcaactct acgtctttgt ttcgttttct gttctgcgcc gttacagatc
     5221 caagctgtga ccggcgccta cgctagacgc caccatggag agcgacgaga gcggcctgcc
     5281 cgccatggag atcgagtgcc gcatcaccgg caccctgaac ggcgtggagt tcgagctggt
     5341 gggcggcgga gagggcaccc ccaagcaggg ccgcatgacc aacaagatga agagcaccaa
     5401 aggcgccctg accttcagcc cctacctgct gagccacgtg atgggctacg gcttctacca
     5461 cttcggcacc taccccagcg gctacgagaa ccccttcctg cacgccatca acaacggcgg
     5521 ctacaccaac acccgcatcg agaagtacga ggacggcggc gtgctgcacg tgagcttcag
     5581 ctaccgctac gaggccggcc gcgtgatcgg cgacttcaag gtggtgggca ccggcttccc
     5641 cgaggacagc gtgatcttca ccgacaagat catccgcagc aacgccaccg tggagcacct
     5701 gcaccccatg ggcgataacg tgctggtggg cagcttcgcc cgcaccttca gcctgcgcga
     5761 cggcggctac tacagcttcg tggtggacag ccacatgcac ttcaagagcg ccatccaccc
     5821 cagcatcctg cagaacgggg gccccatgtt cgccttccgc cgcgtggagg agctgcacag
     5881 caacaccgag ctgggcatcg tggagtacca gcacgccttc aagaccccca tcgccttcgc
     5941 cagatcccgc gctcagtcgt ccaattctgc cgtggacggc accgccggac ccggctccac
     6001 cggatctcgc gagggcagag gaagtcttct aacatgcggt gacgtggagg agaatcccgg
     6061 ccctgccacc atggctgaac aagatggatt gcacgcaggt tctccggccg cttgggtgga
     6121 gaggctattc ggctatgact gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt
     6181 ccggctgtca gcgcaggggc gcccggttct ttttgtcaag accgacctgt ccggtgccct
     6241 gaatgaactg caggacgagg cagcgcggct atcgtggctg gccacgacgg gcgttccttg
     6301 cgcagctgtg ctcgacgttg tcactgaagc gggaagggac tggctgctat tgggcgaagt
     6361 gccggggcag gatctcctgt catctcacct tgctcctgcc gagaaagtat ccatcatggc
     6421 tgatgcaatg cggcggctgc atacgcttga tccggctacc tgcccattcg accaccaagc
     6481 gaaacatcgc atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg atcaggatga
     6541 tctggacgaa gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgcg
     6601 catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat
     6661 ggtggaaaat ggccgctttt ctggattcat cgactgtggc cggctgggtg tggcggaccg
     6721 ctatcaggac atagcgttgg ctacccgtga tattgctgaa gagcttggcg gcgaatgggc
     6781 tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca tcgccttcta
     6841 tcgccttctt gacgagttct tctgagtcga caatcaacct ctggattaca aaatttgtga
     6901 aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt
     6961 aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa
     7021 atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt
     7081 gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct
     7141 cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg
     7201 ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc
     7261 ggggaaatca tcgtcctttc cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg
     7321 gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct
     7381 gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc
     7441 cctttgggcc gcctccccgc ggtaccttta agaccaatga cttacaaggc agctgtagat
     7501 cttagccact ttttaaaaga aaagggggga ctggaagggc taattcactc ccaacgaaga
     7561 taagatctgc tttttgcttg tactgggtct ctctggttag accagatctg agcctgggag
     7621 ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt
     7681 caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt
     7741 tagtcagtgt ggaaaatctc tagcagtagt agttcatgtc atcttattat tcagtattta
     7801 taacttgcaa agaaatgaat atcagagagt gagaggaact tgtttattgc agcttataat
     7861 ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat
     7921 tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggct ctagctatcc
     7981 cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta
     8041 tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct
     8101 tttttggagg cctagacttt tgcagagacg gcccaaattc gtaatcatgg tcatagctgt
     8161 ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa
     8221 agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac
     8281 tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg
     8341 cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc
     8401 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
     8461 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
     8521 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
     8581 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
     8641 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
     8701 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
     8761 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
     8821 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
     8881 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
     8941 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat
     9001 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
     9061 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
     9121 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
     9181 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct
     9241 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt
     9301 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
     9361 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac
     9421 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat
     9481 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
     9541 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
     9601 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta
     9661 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt
     9721 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag
     9781 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa
     9841 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc
     9901 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt
     9961 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc
    10021 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta
    10081 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa
    10141 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca
    10201 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
    10261 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa gaaaccatta
    10321 ttatcatgac attaacctat aaaaataggc gtatcacgag gccctttcgt ctcgcgcgtt
    10381 tcggtgatga cggtgaaaac ctctgacaca tgcagctccc ggagacggtc acagcttgtc
    10441 tgtaagcgga tgccgggagc agacaagccc gtcagggcgc gtcagcgggt gttggcgggt
    10501 gtcggggctg gcttaactat gcggcatcag agcagattgt actgagagtg caccatatgc
    10561 ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg catcaggcgc cattcgccat
    10621 tcaggctgcg caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc
    10681 tggcgaaagg gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt
    10741 cacgacgttg taaaacgacg gccagtgcca agctg
//
