LOCUS       V039609                10784 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V039609
VERSION     V039609
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2303..4621
                     /label="CPT1A(NM_001876)"
                     /note="CPT1A(NM_001876)"
                     /gene="CPT1A"
     CDS             4628..4693
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        4737..4948
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             4961..5229
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      5258..5267
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             5288..5950
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             6020..6073
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             6080..6874
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MAEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     misc_feature    6881..7469
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7541..7774
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    7846..7967
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      7986..8121
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(8159..8175)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8183..8199
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8207..8237)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8252..8273
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8561..9149)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9320..10180)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10181..10285)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     10759..10775
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc gaattcgcca ccatggcaga agctcaccaa gctgtggcct ttcagttcac
     2341 ggtcactccg gacgggattg acctgcggct gagccatgaa gctcttagac aaatctatct
     2401 ctctggactt cattcctgga aaaagaagtt catcagattc aagaacggca tcatcactgg
     2461 cgtgtacccg gcaagcccct ccagttggct tatcgtggtg gtgggcgtga tgacaacgat
     2521 gtacgccaag atcgacccct cgttaggaat aattgcaaaa atcaatcgga ctctggaaac
     2581 ggccaactgc atgtccagcc agacgaagaa cgtggtcagc ggcgtgctgt ttggcaccgg
     2641 cctgtgggtg gccctcatcg tcaccatgcg ctactccctg aaagtgctgc tctcctacca
     2701 cgggtggatg ttcactgagc acggcaagat gagtcgtgcc accaagatct ggatgggtat
     2761 ggtcaagatc ttttcaggcc gaaaacccat gttgtacagc ttccagacat cgctgcctcg
     2821 cctgccggtc ccggctgtca aagacactgt gaacaggtat ctacagtcgg tgaggcctct
     2881 tatgaaggaa gaagacttca aacggatgac agcacttgct caagattttg ctgtcggtct
     2941 tggaccaaga ttacagtggt atttgaagtt aaaatcctgg tgggctacaa attacgtgag
     3001 cgactggtgg gaggagtaca tctacctccg aggacgaggg ccgctcatgg tgaacagcaa
     3061 ctattatgcc atggatctgc tgtatatcct tccaactcac attcaggcag caagagccgg
     3121 caacgccatc catgccatcc tgctttacag gcgcaaactg gaccgggagg aaatcaaacc
     3181 aattcgtctt ttgggatcca cgattccact ctgctccgct cagtgggagc ggatgtttaa
     3241 tacttcccgg atcccaggag aggagacaga caccatccag cacatgagag acagcaagca
     3301 catcgtcgtg taccatcgag gacgctactt caaggtctgg ctctaccatg atgggcggct
     3361 gctgaagccc cgggagatgg agcagcagat gcagaggatc ctggacaata cctcggagcc
     3421 tcagcccggg gaggccaggc tggcagccct caccgcagga gacagagttc cctgggccag
     3481 gtgtcgtcag gcctattttg gacgtgggaa aaataagcag tctcttgatg ctgtggagaa
     3541 agcagcgttc tttgtgacgt tagatgaaac tgaagaagga tacagaagtg aagacccgga
     3601 tacgtcaatg gacagctacg ccaaatctct actacacggc cgatgttacg acaggtggtt
     3661 tgacaagtcg ttcacgtttg ttgtcttcaa aaacgggaag atgggcctca acgctgaaca
     3721 ctcctgggca gatgcgccga tcgtggccca cctttgggag tacgtcatgt ccattgacag
     3781 cctccagctg ggctatgcgg aggatgggca ctgcaaaggc gacatcaatc cgaacattcc
     3841 gtaccccacc aggctgcagt gggacatccc gggggaatgt caagaggtta tagagacctc
     3901 cctgaacacc gcaaatcttc tggcaaacga cgtggatttc cattccttcc cattcgtagc
     3961 ctttggtaaa ggaatcatca agaaatgtcg cacgagccca gacgcctttg tgcagctggc
     4021 cctccagctg gcgcactaca aggacatggg caagttttgc ctcacatacg aggcctccat
     4081 gacccggctc ttccgagagg ggaggacgga gaccgtgcgc tcctgcacca ctgagtcatg
     4141 cgacttcgtg cgggccatgg tggacccggc ccagacggtg gaacagaggc tgaagttgtt
     4201 caagttggcg tctgagaagc atcagcatat gtatcgcctc gccatgaccg gctctgggat
     4261 cgatcgtcac ctcttctgcc tttacgtggt gtctaaatat ctcgctgtgg agtccccttt
     4321 ccttaaggaa gttttatctg agccttggag attatcaaca agccagaccc ctcagcagca
     4381 agtggagctg tttgacttgg agaataaccc agagtacgtg tccagcggag ggggctttgg
     4441 accggttgct gatgacggct atggtgtgtc gtacatcctt gtgggagaga acctcatcaa
     4501 tttccacatt tcttccaagt tctcttgccc tgagacggat tctcatcgct ttggaaggca
     4561 cctgaaagaa gcaatgactg acatcatcac tttgtttggt ctcagttcta attccaaaaa
     4621 gggatccgac tacaaagacc atgacggtga ttataaagat catgacatcg actacaagga
     4681 tgacgatgac aagtagcggc cgcgaaggat ctgcgatcgc tccggtgccc gtcagtgggc
     4741 agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca attgaacggg
     4801 tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact ggctccgcct
     4861 ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga acgttctttt
     4921 tcgcaacggg tttgccgcca gaacacagct gaagcttcga ggggctcgca tctctccttc
     4981 acgcgcccgc cgccctacct gaggccgcca tccacgccgg ttgagtcgcg ttctgccgcc
     5041 tcccgcctgt ggtgcctcct gaactgcgtc cgccgtctag gtaagtttaa agctcaggtc
     5101 gagaccgggc ctttgtccgg cgctcccttg gagcctacct agactcagcc ggctctccac
     5161 gctttgcctg accctgcttg ctcaactcta cgtctttgtt tcgttttctg ttctgcgccg
     5221 ttacagatcc aagctgtgac cggcgcctac gctagacgcc accatggaga gcgacgagag
     5281 cggcctgccc gccatggaga tcgagtgccg catcaccggc accctgaacg gcgtggagtt
     5341 cgagctggtg ggcggcggag agggcacccc caagcagggc cgcatgacca acaagatgaa
     5401 gagcaccaaa ggcgccctga ccttcagccc ctacctgctg agccacgtga tgggctacgg
     5461 cttctaccac ttcggcacct accccagcgg ctacgagaac cccttcctgc acgccatcaa
     5521 caacggcggc tacaccaaca cccgcatcga gaagtacgag gacggcggcg tgctgcacgt
     5581 gagcttcagc taccgctacg aggccggccg cgtgatcggc gacttcaagg tggtgggcac
     5641 cggcttcccc gaggacagcg tgatcttcac cgacaagatc atccgcagca acgccaccgt
     5701 ggagcacctg caccccatgg gcgataacgt gctggtgggc agcttcgccc gcaccttcag
     5761 cctgcgcgac ggcggctact acagcttcgt ggtggacagc cacatgcact tcaagagcgc
     5821 catccacccc agcatcctgc agaacggggg ccccatgttc gccttccgcc gcgtggagga
     5881 gctgcacagc aacaccgagc tgggcatcgt ggagtaccag cacgccttca agacccccat
     5941 cgccttcgcc agatcccgcg ctcagtcgtc caattctgcc gtggacggca ccgccggacc
     6001 cggctccacc ggatctcgcg agggcagagg aagtcttcta acatgcggtg acgtggagga
     6061 gaatcccggc cctgccacca tggctgaaca agatggattg cacgcaggtt ctccggccgc
     6121 ttgggtggag aggctattcg gctatgactg ggcacaacag acaatcggct gctctgatgc
     6181 cgccgtgttc cggctgtcag cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc
     6241 cggtgccctg aatgaactgc aggacgaggc agcgcggcta tcgtggctgg ccacgacggg
     6301 cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg ggaagggact ggctgctatt
     6361 gggcgaagtg ccggggcagg atctcctgtc atctcacctt gctcctgccg agaaagtatc
     6421 catcatggct gatgcaatgc ggcggctgca tacgcttgat ccggctacct gcccattcga
     6481 ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg atggaagccg gtcttgtcga
     6541 tcaggatgat ctggacgaag agcatcaggg gctcgcgcca gccgaactgt tcgccaggct
     6601 caaggcgcgc atgcccgacg gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc
     6661 gaatatcatg gtggaaaatg gccgcttttc tggattcatc gactgtggcc ggctgggtgt
     6721 ggcggaccgc tatcaggaca tagcgttggc tacccgtgat attgctgaag agcttggcgg
     6781 cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat
     6841 cgccttctat cgccttcttg acgagttctt ctgagtcgac aatcaacctc tggattacaa
     6901 aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata
     6961 cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc
     7021 cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg
     7081 tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac
     7141 ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat
     7201 cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt
     7261 ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg ccacctggat
     7321 tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc
     7381 ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag
     7441 tcggatctcc ctttgggccg cctccccgcg gtacctttaa gaccaatgac ttacaaggca
     7501 gctgtagatc ttagccactt tttaaaagaa aaggggggac tggaagggct aattcactcc
     7561 caacgaagat aagatctgct ttttgcttgt actgggtctc tctggttaga ccagatctga
     7621 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
     7681 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
     7741 agaccctttt agtcagtgtg gaaaatctct agcagtagta gttcatgtca tcttattatt
     7801 cagtatttat aacttgcaaa gaaatgaata tcagagagtg agaggaactt gtttattgca
     7861 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt
     7921 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggctc
     7981 tagctatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa
     8041 ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt
     8101 gaggaggctt ttttggaggc ctagactttt gcagagacgg cccaaattcg taatcatggt
     8161 catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg
     8221 gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt
     8281 tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg
     8341 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg
     8401 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
     8461 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
     8521 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
     8581 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
     8641 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
     8701 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
     8761 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
     8821 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
     8881 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
     8941 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
     9001 ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
     9061 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
     9121 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
     9181 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
     9241 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
     9301 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
     9361 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
     9421 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc
     9481 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
     9541 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
     9601 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt
     9661 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
     9721 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
     9781 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
     9841 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
     9901 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata
     9961 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
    10021 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
    10081 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
    10141 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
    10201 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
    10261 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag
    10321 aaaccattat tatcatgaca ttaacctata aaaataggcg tatcacgagg ccctttcgtc
    10381 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
    10441 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
    10501 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
    10561 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
    10621 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
    10681 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
    10741 tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa gctg
//
