LOCUS       V030240                11294 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V030240
VERSION     V030240
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2303..5131
                     /label="EGFR(NM_001346941)"
                     /note="EGFR(NM_001346941)"
                     /gene="EGFR"
     CDS             5138..5203
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        5247..5458
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             5471..5739
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      5768..5777
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             5798..6460
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             6530..6583
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             6590..7384
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MAEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     misc_feature    7391..7979
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             8051..8284
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    8356..8477
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      8496..8631
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(8669..8685)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8693..8709
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8717..8747)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8762..8783
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(9071..9659)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9830..10690)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10691..10795)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     11269..11285
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc gaattcgcca ccatgcgacc ctccgggacg gccggggcag cgctcctggc
     2341 gctgctggct gcgctctgcc cggcgagtcg ggctctggag gaaaagaaag gtaattatgt
     2401 ggtgacagat cacggctcgt gcgtccgagc ctgtggggcc gacagctatg agatggagga
     2461 agacggcgtc cgcaagtgta agaagtgcga agggccttgc cgcaaagtgt gtaacggaat
     2521 aggtattggt gaatttaaag actcactctc cataaatgct acgaatatta aacacttcaa
     2581 aaactgcacc tccatcagtg gcgatctcca catcctgccg gtggcattta ggggtgactc
     2641 cttcacacat actcctcctc tggatccaca ggaactggat attctgaaaa ccgtaaagga
     2701 aatcacaggg tttttgctga ttcaggcttg gcctgaaaac aggacggacc tccatgcctt
     2761 tgagaaccta gaaatcatac gcggcaggac caagcaacat ggtcagtttt ctcttgcagt
     2821 cgtcagcctg aacataacat ccttgggatt acgctccctc aaggagataa gtgatggaga
     2881 tgtgataatt tcaggaaaca aaaatttgtg ctatgcaaat acaataaact ggaaaaaact
     2941 gtttgggacc tccggtcaga aaaccaaaat tataagcaac agaggtgaaa acagctgcaa
     3001 ggccacaggc caggtctgcc atgccttgtg ctcccccgag ggctgctggg gcccggagcc
     3061 cagggactgc gtctcttgcc ggaatgtcag ccgaggcagg gaatgcgtgg acaagtgcaa
     3121 ccttctggag ggtgagccaa gggagtttgt ggagaactct gagtgcatac agtgccaccc
     3181 agagtgcctg cctcaggcca tgaacatcac ctgcacagga cggggaccag acaactgtat
     3241 ccagtgtgcc cactacattg acggccccca ctgcgtcaag acctgcccgg caggagtcat
     3301 gggagaaaac aacaccctgg tctggaagta cgcagacgcc ggccatgtgt gccacctgtg
     3361 ccatccaaac tgcacctacg gatgcactgg gccaggtctt gaaggctgtc caacgaatgg
     3421 gcctaagatc ccgtccatcg ccactgggat ggtgggggcc ctcctcttgc tgctggtggt
     3481 ggccctgggg atcggcctct tcatgcgaag gcgccacatc gttcggaagc gcacgctgcg
     3541 gaggctgctg caggagaggg agcttgtgga gcctcttaca cccagtggag aagctcccaa
     3601 ccaagctctc ttgaggatct tgaaggaaac tgaattcaaa aagatcaaag tgctgggctc
     3661 cggtgcgttc ggcacggtgt ataagggact ctggatccca gaaggtgaga aagttaaaat
     3721 tcccgtcgct atcaaggaat taagagaagc aacatctccg aaagccaaca aggaaatcct
     3781 cgatgaagcc tacgtgatgg ccagcgtgga caacccccac gtgtgccgcc tgctgggcat
     3841 ctgcctcacc tccaccgtgc agctcatcac gcagctcatg cccttcggct gcctcctgga
     3901 ctatgtccgg gaacacaaag acaatattgg ctcccagtac ctgctcaact ggtgtgtgca
     3961 gatcgcaaag ggcatgaact acttggagga ccgtcgcttg gtgcaccgcg acctggcagc
     4021 caggaacgta ctggtgaaaa caccgcagca tgtcaagatc acagattttg ggctggccaa
     4081 actgctgggt gcggaagaga aagaatacca tgcagaagga ggcaaagtgc ctatcaagtg
     4141 gatggcattg gaatcaattt tacacagaat ctatacccac cagagtgatg tctggagcta
     4201 cggggtgact gtttgggagt tgatgacctt tggatccaag ccatatgacg gaatccctgc
     4261 cagcgagatc tcctccatcc tggagaaagg agaacgcctc cctcagccac ccatatgtac
     4321 catcgatgtc tacatgatca tggtcaagtg ctggatgata gacgcagata gtcgcccaaa
     4381 gttccgtgag ttgatcatcg aattctccaa aatggcccga gacccccagc gctaccttgt
     4441 cattcagggg gatgaaagaa tgcatttgcc aagtcctaca gactccaact tctaccgtgc
     4501 cctgatggat gaagaagaca tggacgacgt ggtggatgcc gacgagtacc tcatcccaca
     4561 gcagggcttc ttcagcagcc cctccacgtc acggactccc ctcctgagct ctctgagtgc
     4621 aaccagcaac aattccaccg tggcttgcat tgatagaaat gggctgcaaa gctgtcccat
     4681 caaggaagac agcttcttgc agcgatacag ctcagacccc acaggcgcct tgactgagga
     4741 cagcatagac gacaccttcc tcccagtgcc tgaatacata aaccagtccg ttcccaaaag
     4801 gcccgctggc tctgtgcaga atcctgtcta tcacaatcag cctctgaacc ccgcgcccag
     4861 cagagaccca cactaccagg acccccacag cactgcagtg ggcaaccccg agtatctcaa
     4921 cactgtccag cccacctgtg tcaacagcac attcgacagc cctgcccact gggcccagaa
     4981 aggcagccac caaattagcc tggacaaccc tgactaccag caggacttct ttcccaagga
     5041 agccaagcca aatggcatct ttaagggctc cacagctgaa aatgcagaat acctaagggt
     5101 cgcgccacaa agcagtgaat ttattggagc aggatccgac tacaaagacc atgacggtga
     5161 ttataaagat catgacatcg actacaagga tgacgatgac aagtagcggc cgcgaaggat
     5221 ctgcgatcgc tccggtgccc gtcagtgggc agagcgcaca tcgcccacag tccccgagaa
     5281 gttgggggga ggggtcggca attgaacggg tgcctagaga aggtggcgcg gggtaaactg
     5341 ggaaagtgat gtcgtgtact ggctccgcct ttttcccgag ggtgggggag aaccgtatat
     5401 aagtgcagta gtcgccgtga acgttctttt tcgcaacggg tttgccgcca gaacacagct
     5461 gaagcttcga ggggctcgca tctctccttc acgcgcccgc cgccctacct gaggccgcca
     5521 tccacgccgg ttgagtcgcg ttctgccgcc tcccgcctgt ggtgcctcct gaactgcgtc
     5581 cgccgtctag gtaagtttaa agctcaggtc gagaccgggc ctttgtccgg cgctcccttg
     5641 gagcctacct agactcagcc ggctctccac gctttgcctg accctgcttg ctcaactcta
     5701 cgtctttgtt tcgttttctg ttctgcgccg ttacagatcc aagctgtgac cggcgcctac
     5761 gctagacgcc accatggaga gcgacgagag cggcctgccc gccatggaga tcgagtgccg
     5821 catcaccggc accctgaacg gcgtggagtt cgagctggtg ggcggcggag agggcacccc
     5881 caagcagggc cgcatgacca acaagatgaa gagcaccaaa ggcgccctga ccttcagccc
     5941 ctacctgctg agccacgtga tgggctacgg cttctaccac ttcggcacct accccagcgg
     6001 ctacgagaac cccttcctgc acgccatcaa caacggcggc tacaccaaca cccgcatcga
     6061 gaagtacgag gacggcggcg tgctgcacgt gagcttcagc taccgctacg aggccggccg
     6121 cgtgatcggc gacttcaagg tggtgggcac cggcttcccc gaggacagcg tgatcttcac
     6181 cgacaagatc atccgcagca acgccaccgt ggagcacctg caccccatgg gcgataacgt
     6241 gctggtgggc agcttcgccc gcaccttcag cctgcgcgac ggcggctact acagcttcgt
     6301 ggtggacagc cacatgcact tcaagagcgc catccacccc agcatcctgc agaacggggg
     6361 ccccatgttc gccttccgcc gcgtggagga gctgcacagc aacaccgagc tgggcatcgt
     6421 ggagtaccag cacgccttca agacccccat cgccttcgcc agatcccgcg ctcagtcgtc
     6481 caattctgcc gtggacggca ccgccggacc cggctccacc ggatctcgcg agggcagagg
     6541 aagtcttcta acatgcggtg acgtggagga gaatcccggc cctgccacca tggctgaaca
     6601 agatggattg cacgcaggtt ctccggccgc ttgggtggag aggctattcg gctatgactg
     6661 ggcacaacag acaatcggct gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg
     6721 cccggttctt tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc aggacgaggc
     6781 agcgcggcta tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt
     6841 cactgaagcg ggaagggact ggctgctatt gggcgaagtg ccggggcagg atctcctgtc
     6901 atctcacctt gctcctgccg agaaagtatc catcatggct gatgcaatgc ggcggctgca
     6961 tacgcttgat ccggctacct gcccattcga ccaccaagcg aaacatcgca tcgagcgagc
     7021 acgtactcgg atggaagccg gtcttgtcga tcaggatgat ctggacgaag agcatcaggg
     7081 gctcgcgcca gccgaactgt tcgccaggct caaggcgcgc atgcccgacg gcgaggatct
     7141 cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc
     7201 tggattcatc gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc
     7261 tacccgtgat attgctgaag agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta
     7321 cggtatcgcc gctcccgatt cgcagcgcat cgccttctat cgccttcttg acgagttctt
     7381 ctgagtcgac aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa
     7441 ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat
     7501 tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta
     7561 tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc
     7621 aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt
     7681 ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg
     7741 ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc
     7801 ttggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc
     7861 ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct
     7921 tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgcg
     7981 gtacctttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa
     8041 aaggggggac tggaagggct aattcactcc caacgaagat aagatctgct ttttgcttgt
     8101 actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac
     8161 ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg
     8221 ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct
     8281 agcagtagta gttcatgtca tcttattatt cagtatttat aacttgcaaa gaaatgaata
     8341 tcagagagtg agaggaactt gtttattgca gcttataatg gttacaaata aagcaatagc
     8401 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa
     8461 ctcatcaatg tatcttatca tgtctggctc tagctatccc gcccctaact ccgcccagtt
     8521 ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg
     8581 cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc ctagactttt
     8641 gcagagacgg cccaaattcg taatcatggt catagctgtt tcctgtgtga aattgttatc
     8701 cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct
     8761 aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa
     8821 acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta
     8881 ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
     8941 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
     9001 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
     9061 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
     9121 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
     9181 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
     9241 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
     9301 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
     9361 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
     9421 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
     9481 agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga
     9541 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
     9601 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
     9661 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
     9721 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
     9781 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
     9841 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
     9901 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa
     9961 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg
    10021 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
    10081 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
    10141 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
    10201 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct
    10261 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
    10321 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
    10381 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
    10441 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
    10501 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
    10561 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
    10621 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
    10681 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
    10741 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
    10801 ttccccgaaa agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata
    10861 aaaataggcg tatcacgagg ccctttcgtc tcgcgcgttt cggtgatgac ggtgaaaacc
    10921 tctgacacat gcagctcccg gagacggtca cagcttgtct gtaagcggat gccgggagca
    10981 gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg tcggggctgg cttaactatg
    11041 cggcatcaga gcagattgta ctgagagtgc accatatgcg gtgtgaaata ccgcacagat
    11101 gcgtaaggag aaaataccgc atcaggcgcc attcgccatt caggctgcgc aactgttggg
    11161 aagggcgatc ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg
    11221 caaggcgatt aagttgggta acgccagggt tttcccagtc acgacgttgt aaaacgacgg
    11281 ccagtgccaa gctg
//
