LOCUS       V037321                10256 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V037321
VERSION     V037321
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2303..4069
                     /label="IL4I1(NM_172374)"
                     /note="IL4I1(NM_172374)"
                     /gene="IL4I1"
     CDS             4076..4165
                     /codon_start=1
                     /product="3 tandem Myc epitope tags"
                     /transl_table=1
                     /translation="EQKLISEEDLEQKLISEEDLEQKLISEEDL"
                     /label="3xMyc"
     promoter        4209..4420
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             4433..4701
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      4730..4739
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             4760..5422
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             5492..5545
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             5552..6346
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MAEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     misc_feature    6353..6941
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7013..7246
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    7318..7439
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      7458..7593
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(7631..7647)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    7655..7671
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(7679..7709)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    7724..7745
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8033..8621)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(8792..9652)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(9653..9757)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     10231..10247
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc gaattcgcca ccatgcccaa cgatgacttc tgtcctgggc taaccataaa
     2341 ggccatgggt gctgagagag ccccccagag gcagccatgc accctgcacc tcctcgtcct
     2401 cgtccccatc ctcctcagcc tggtggcctc ccaggactgg aaggctgaac gcagccaaga
     2461 ccccttcgag aaatgcatgc aggatcctga ctatgagcag ctgctcaagg tggtgacctg
     2521 ggggctcaat cggaccctga agccccagag ggtgattgtg gttggcgctg gtgtggccgg
     2581 gctggtggcc gccaaggtgc tcagcgatgc tggacacaag gtcaccatcc tggaggcaga
     2641 taacaggatc gggggccgca tcttcaccta ccgggaccag aacacgggct ggattgggga
     2701 gctgggagcc atgcgcatgc ccagctctca caggatcctc cacaagctct gccagggcct
     2761 ggggctcaac ctgaccaagt tcacccagta cgacaagaac acgtggacgg aggtgcacga
     2821 agtgaagctg cgcaactatg tggtggagaa ggtgcccgag aagctgggct acgccttgcg
     2881 tccccaggaa aagggccact cgcccgaaga catctaccag atggctctca accaggccct
     2941 caaagacctc aaggcactgg gctgcagaaa ggcgatgaag aagtttgaaa ggcacacgct
     3001 cttggaatat cttctcgggg aggggaacct gagccggccg gccgtgcagc ttctgggaga
     3061 cgtgatgtcc gaggatggct tcttctatct cagcttcgcc gaggccctcc gggcccacag
     3121 ctgcctcagc gacagactcc agtacagccg catcgtgggt ggctgggacc tgctgccgcg
     3181 cgcgctgctg agctcgctgt ccgggcttgt gctgttgaac gcgcccgtgg tggcgatgac
     3241 ccagggaccg cacgatgtgc acgtgcagat cgagacctct cccccggcgc ggaatctgaa
     3301 ggtgctgaag gccgacgtgg tgctgctgac ggcgagcgga ccggcggtga agcgcatcac
     3361 cttctcgccg ccgctgcccc gccacatgca ggaggcgctg cggaggctgc actacgtgcc
     3421 ggccaccaag gtgttcctaa gcttccgcag gcccttctgg cgcgaggagc acattgaagg
     3481 cggccactca aacaccgatc gcccgtcgcg catgattttc tacccgccgc cgcgcgaggg
     3541 cgcgctgctg ctggcctcgt acacgtggtc ggacgcggcg gcagcgttcg ccggcttgag
     3601 ccgggaagag gcgttgcgct tggcgctcga cgacgtggcg gcattgcacg ggcctgtcgt
     3661 gcgccagctc tgggacggca ccggcgtcgt caagcgttgg gcggaggacc agcacagcca
     3721 gggtggcttt gtggtacagc cgccggcgct ctggcaaacc gaaaaggatg actggacggt
     3781 cccttatggc cgcatctact ttgccggcga gcacaccgcc tacccgcacg gctgggtgga
     3841 gacggcggtc aagtcggcgc tgcgcgccgc catcaagatc aacagccgga aggggcctgc
     3901 atcggacacg gccagccccg aggggcacgc atctgacatg gaggggcagg ggcatgtgca
     3961 tggggtggcc agcagcccct cgcatgacct ggcaaaggaa gaaggcagcc accctccagt
     4021 ccaaggccag ttatctctcc aaaacacgac ccacacgagg acctcgcatg gatccgaaca
     4081 aaaactaata tcagaggaag atttggaaca gaaactaatc agtgaagaag atttagagca
     4141 aaagttaatt tccgaggagg acttatagcg gccgcgaagg atctgcgatc gctccggtgc
     4201 ccgtcagtgg gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg
     4261 caattgaacg ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta
     4321 ctggctccgc ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt
     4381 gaacgttctt tttcgcaacg ggtttgccgc cagaacacag ctgaagcttc gaggggctcg
     4441 catctctcct tcacgcgccc gccgccctac ctgaggccgc catccacgcc ggttgagtcg
     4501 cgttctgccg cctcccgcct gtggtgcctc ctgaactgcg tccgccgtct aggtaagttt
     4561 aaagctcagg tcgagaccgg gcctttgtcc ggcgctccct tggagcctac ctagactcag
     4621 ccggctctcc acgctttgcc tgaccctgct tgctcaactc tacgtctttg tttcgttttc
     4681 tgttctgcgc cgttacagat ccaagctgtg accggcgcct acgctagacg ccaccatgga
     4741 gagcgacgag agcggcctgc ccgccatgga gatcgagtgc cgcatcaccg gcaccctgaa
     4801 cggcgtggag ttcgagctgg tgggcggcgg agagggcacc cccaagcagg gccgcatgac
     4861 caacaagatg aagagcacca aaggcgccct gaccttcagc ccctacctgc tgagccacgt
     4921 gatgggctac ggcttctacc acttcggcac ctaccccagc ggctacgaga accccttcct
     4981 gcacgccatc aacaacggcg gctacaccaa cacccgcatc gagaagtacg aggacggcgg
     5041 cgtgctgcac gtgagcttca gctaccgcta cgaggccggc cgcgtgatcg gcgacttcaa
     5101 ggtggtgggc accggcttcc ccgaggacag cgtgatcttc accgacaaga tcatccgcag
     5161 caacgccacc gtggagcacc tgcaccccat gggcgataac gtgctggtgg gcagcttcgc
     5221 ccgcaccttc agcctgcgcg acggcggcta ctacagcttc gtggtggaca gccacatgca
     5281 cttcaagagc gccatccacc ccagcatcct gcagaacggg ggccccatgt tcgccttccg
     5341 ccgcgtggag gagctgcaca gcaacaccga gctgggcatc gtggagtacc agcacgcctt
     5401 caagaccccc atcgccttcg ccagatcccg cgctcagtcg tccaattctg ccgtggacgg
     5461 caccgccgga cccggctcca ccggatctcg cgagggcaga ggaagtcttc taacatgcgg
     5521 tgacgtggag gagaatcccg gccctgccac catggctgaa caagatggat tgcacgcagg
     5581 ttctccggcc gcttgggtgg agaggctatt cggctatgac tgggcacaac agacaatcgg
     5641 ctgctctgat gccgccgtgt tccggctgtc agcgcagggg cgcccggttc tttttgtcaa
     5701 gaccgacctg tccggtgccc tgaatgaact gcaggacgag gcagcgcggc tatcgtggct
     5761 ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga
     5821 ctggctgcta ttgggcgaag tgccggggca ggatctcctg tcatctcacc ttgctcctgc
     5881 cgagaaagta tccatcatgg ctgatgcaat gcggcggctg catacgcttg atccggctac
     5941 ctgcccattc gaccaccaag cgaaacatcg catcgagcga gcacgtactc ggatggaagc
     6001 cggtcttgtc gatcaggatg atctggacga agagcatcag gggctcgcgc cagccgaact
     6061 gttcgccagg ctcaaggcgc gcatgcccga cggcgaggat ctcgtcgtga cccatggcga
     6121 tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt tctggattca tcgactgtgg
     6181 ccggctgggt gtggcggacc gctatcagga catagcgttg gctacccgtg atattgctga
     6241 agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga
     6301 ttcgcagcgc atcgccttct atcgccttct tgacgagttc ttctgagtcg acaatcaacc
     6361 tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac
     6421 gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt
     6481 cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt
     6541 tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg
     6601 cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac
     6661 ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac
     6721 tgacaattcc gtggtgttgt cggggaaatc atcgtccttt ccttggctgc tcgcctgtgt
     6781 tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc
     6841 ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg
     6901 ccctcagacg agtcggatct ccctttgggc cgcctccccg cggtaccttt aagaccaatg
     6961 acttacaagg cagctgtaga tcttagccac tttttaaaag aaaagggggg actggaaggg
     7021 ctaattcact cccaacgaag ataagatctg ctttttgctt gtactgggtc tctctggtta
     7081 gaccagatct gagcctggga gctctctggc taactaggga acccactgct taagcctcaa
     7141 taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac
     7201 tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtag tagttcatgt
     7261 catcttatta ttcagtattt ataacttgca aagaaatgaa tatcagagag tgagaggaac
     7321 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat
     7381 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat
     7441 catgtctggc tctagctatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc
     7501 atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat
     7561 tccagaagta gtgaggaggc ttttttggag gcctagactt ttgcagagac ggcccaaatt
     7621 cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca
     7681 acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca
     7741 cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc
     7801 attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt
     7861 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
     7921 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
     7981 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
     8041 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
     8101 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
     8161 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
     8221 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
     8281 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
     8341 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
     8401 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
     8461 gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
     8521 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
     8581 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
     8641 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
     8701 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
     8761 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta
     8821 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa
     8881 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac
     8941 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa
     9001 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
     9061 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg
     9121 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag
     9181 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg
     9241 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc
     9301 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat
     9361 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata
     9421 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa
     9481 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca
     9541 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
     9601 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
     9661 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
     9721 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
     9781 ctgacgtcta agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga
     9841 ggccctttcg tctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac atgcagctcc
     9901 cggagacggt cacagcttgt ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg
     9961 cgtcagcggg tgttggcggg tgtcggggct ggcttaacta tgcggcatca gagcagattg
    10021 tactgagagt gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc
    10081 gcatcaggcg ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg
    10141 cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg
    10201 taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc aagctg
//
