LOCUS       V031815                11181 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V031815
VERSION     V031815
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1798..1915
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2005..2208
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2285..3298
                     /label="Stbd1(NM_175096)"
                     /note="Stbd1(NM_175096)"
                     /gene="Stbd1"
     CDS             3305..3370
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     CDS             3377..3433
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from porcine teschovirus-1
                     polyprotein"
                     /transl_table=1
                     /translation="ATNFSLLKQAGDVEENPGP"
                     /label="P2A"
     CDS             3446..5098
                     /EC_number=""
                     /codon_start=1
                     /experiment=""
                     /gene="luc"
                     /product="firefly luciferase"
                     /protein_id=""
                     /transl_table=1
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVNITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMNISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKSKL*"
                     /label="luciferase"
     promoter        5145..5356
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             5369..5637
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      5666..5675
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             5696..6358
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             6428..6481
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             6476..7276
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="GPMIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQG
                     RPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL
                     LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEE
                     HQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQD
                     IALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     misc_feature    7277..7864
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7938..8171
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    8243..8364
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      8383..8518
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(8556..8572)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8580..8596
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8604..8634)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8649..8670
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8958..9546)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9717..10577)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10578..10682)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     11156..11172
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggttaacttt
     1801 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc
     1861 aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa attttatcga
     1921 tactagtatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac
     1981 gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga
     2041 tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg
     2101 ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg
     2161 caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctcg tttagtgaac
     2221 cgtcagatcg cctggagacg ccatccacgc tgttttgacc tccatagaag attctagagc
     2281 caccatgggc gccgtctggt cagccctgct ggtcggcggg ggtctagctg gagcgctcat
     2341 cctgtggctg ctgcggggag actctggggc cccggggaaa gacggggttg cggagccgcc
     2401 gcagaagggc gcacctcctg gggaggctgc ggccccggga gacggtccgg gtggtggtgg
     2461 cagtggcggc ctgagccctg aaccttccga tcgggagctg gtctccaaag cagagcatct
     2521 tcgagaaagc aacggacatt tgatttctga gagcaaagat cttggtaacc tgccggaagc
     2581 acagcggctg cagaatgttg gagcagactg ggtcaatgcc agagagtttg ttcctgttgg
     2641 gaagattcca gacacacact ccagggccga ctctgaagcg gcaagaaatc aaagcccagg
     2701 atctcatgga ggagaatgga gactccccaa aggacaagaa acagctgtca aagtagctgg
     2761 cagtgtggcc gcaaagctgc cctccagcag cctgcttgtg gacagagcta aagcagtcag
     2821 tcaggaccag gcaggccacg aggactggga agtggtgtct aggcactcat cttgggggag
     2881 tgttggtttg ggtggcagtc ttgaggcttc taggttaagt ctaaatcaga gaatggacga
     2941 cagcacaaac agtcttgtgg gaggaagagg ctgggaagta gatgggaaag tggcatctct
     3001 gaaacctcaa caggtcagca tccagttcca ggtgcactac accacaaaca ccgatgtgca
     3061 gttcattgca gtgactggag accatgagag ccttgggaga tggaacacat acatcccact
     3121 ccactactgc aaagacgggc tctggtctca ttctgtcttc ctgcctgcag acacagtggt
     3181 ggagtggaag ttcgtgttgg tagagaataa ggaagttact cgttgggaag aatgcagcaa
     3241 tagattcctg cagactggcc atgaggataa agtggttcat gggtggtggg ggattcacct
     3301 cgaggactac aaagaccatg acggtgatta taaagatcat gacatcgact acaaggatga
     3361 cgatgacaag cggatcgcta ctaacttcag cctgctgaag caggctggag acgtggagga
     3421 gaaccctgga cctgaattcg ccaccatgga agacgccaaa aacataaaga aaggcccggc
     3481 gccattctat ccgctagagg atggaaccgc tggagagcaa ctgcataagg ctatgaagag
     3541 atacgccctg gttcctggaa caattgcttt tacagatgca catatcgagg tgaacatcac
     3601 gtacgcggaa tacttcgaaa tgtccgttcg gttggcagaa gctatgaaac gatatgggct
     3661 gaatacaaat cacagaatcg tcgtatgcag tgaaaactct cttcaattct ttatgccggt
     3721 gttgggcgcg ttatttatcg gagttgcagt tgcgcccgcg aacgacattt ataatgaacg
     3781 tgaattgctc aacagtatga acatttcgca gcctaccgta gtgtttgttt ccaaaaaggg
     3841 gttgcaaaaa attttgaacg tgcaaaaaaa attaccaata atccagaaaa ttattatcat
     3901 ggattctaaa acggattacc agggatttca gtcgatgtac acgttcgtca catctcatct
     3961 acctcccggt tttaatgaat acgattttgt accagagtcc tttgatcgtg acaaaacaat
     4021 tgcactgata atgaactcct ctggatctac tgggttacct aagggtgtgg cccttccgca
     4081 tagaactgcc tgcgtcagat tctcgcatgc cagagatcct atttttggca atcaaatcat
     4141 tccggatact gcgattttaa gtgttgttcc attccatcac ggttttggaa tgtttactac
     4201 actcggatat ttgatatgtg gatttcgagt cgtcttaatg tatagatttg aagaagagct
     4261 gtttttacga tcccttcagg attacaaaat tcaaagtgcg ttgctagtac caaccctatt
     4321 ttcattcttc gccaaaagca ctctgattga caaatacgat ttatctaatt tacacgaaat
     4381 tgcttctggg ggcgcacctc tttcgaaaga agtcggggaa gcggttgcaa aacgcttcca
     4441 tcttccaggg atacgacaag gatatgggct cactgagact acatcagcta ttctgattac
     4501 acccgagggg gatgataaac cgggcgcggt cggtaaagtt gttccatttt ttgaagcgaa
     4561 ggttgtggat ctggataccg ggaaaacgct gggcgttaat cagagaggcg aattatgtgt
     4621 cagaggacct atgattatgt ccggttatgt aaacaatccg gaagcgacca acgccttgat
     4681 tgacaaggat ggatggctac attctggaga catagcttac tgggacgaag acgaacactt
     4741 cttcatagtt gaccgcttga agtctttaat taaatacaaa ggataccagg tggcccccgc
     4801 tgaattggag tcgatattgt tacaacaccc caacatcttc gacgcgggcg tggcaggtct
     4861 tcccgacgat gacgccggtg aacttcccgc cgccgttgtt gttttggagc acggaaagac
     4921 gatgacggaa aaagagatcg tggattacgt cgccagtcaa gtaacaaccg cgaaaaagtt
     4981 gcgcggagga gttgtgtttg tggacgaagt accgaaaggt cttaccggaa aactcgacgc
     5041 aagaaaaatc agagagatcc tcataaaggc caagaagggc ggaaagtcca aattgtgagg
     5101 atccgcggcc gcaaggatct gcgatcgctc cggtgcccgt cagtgggcag agcgcacatc
     5161 gcccacagtc cccgagaagt tggggggagg ggtcggcaat tgaacgggtg cctagagaag
     5221 gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg ctccgccttt ttcccgaggg
     5281 tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac gttctttttc gcaacgggtt
     5341 tgccgccaga acacagctga agcttcgagg ggctcgcatc tctccttcac gcgcccgccg
     5401 ccctacctga ggccgccatc cacgccggtt gagtcgcgtt ctgccgcctc ccgcctgtgg
     5461 tgcctcctga actgcgtccg ccgtctaggt aagtttaaag ctcaggtcga gaccgggcct
     5521 ttgtccggcg ctcccttgga gcctacctag actcagccgg ctctccacgc tttgcctgac
     5581 cctgcttgct caactctacg tctttgtttc gttttctgtt ctgcgccgtt acagatccaa
     5641 gctgtgaccg gcgcctacgc tagacgccac catggagagc gacgagagcg gcctgcccgc
     5701 catggagatc gagtgccgca tcaccggcac cctgaacggc gtggagttcg agctggtggg
     5761 cggcggagag ggcaccccca agcagggccg catgaccaac aagatgaaga gcaccaaagg
     5821 cgccctgacc ttcagcccct acctgctgag ccacgtgatg ggctacggct tctaccactt
     5881 cggcacctac cccagcggct acgagaaccc cttcctgcac gccatcaaca acggcggcta
     5941 caccaacacc cgcatcgaga agtacgagga cggcggcgtg ctgcacgtga gcttcagcta
     6001 ccgctacgag gccggccgcg tgatcggcga cttcaaggtg gtgggcaccg gcttccccga
     6061 ggacagcgtg atcttcaccg acaagatcat ccgcagcaac gccaccgtgg agcacctgca
     6121 ccccatgggc gataacgtgc tggtgggcag cttcgcccgc accttcagcc tgcgcgacgg
     6181 cggctactac agcttcgtgg tggacagcca catgcacttc aagagcgcca tccaccccag
     6241 catcctgcag aacgggggcc ccatgttcgc cttccgccgc gtggaggagc tgcacagcaa
     6301 caccgagctg ggcatcgtgg agtaccagca cgccttcaag acccccatcg ccttcgccag
     6361 atcccgcgct cagtcgtcca attctgccgt ggacggcacc gccggacccg gctccaccgg
     6421 atctcgcgag ggcagaggaa gtcttctaac atgcggtgac gtggaggaga atcccggccc
     6481 tatgattgaa caagatggat tgcacgcagg ttctccggcc gcttgggtgg agaggctatt
     6541 cggctatgac tgggcacaac agacaatcgg ctgctctgat gccgccgtgt tccggctgtc
     6601 agcgcagggg cgcccggttc tttttgtcaa gaccgacctg tccggtgccc tgaatgaact
     6661 gcaggacgag gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt
     6721 gctcgacgtt gtcactgaag cgggaaggga ctggctgcta ttgggcgaag tgccggggca
     6781 ggatctcctg tcatctcacc ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat
     6841 gcggcggctg catacgcttg atccggctac ctgcccattc gaccaccaag cgaaacatcg
     6901 catcgagcga gcacgtactc ggatggaagc cggtcttgtc gatcaggatg atctggacga
     6961 agagcatcag gggctcgcgc cagccgaact gttcgccagg ctcaaggcgc gcatgcccga
     7021 cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca tggtggaaaa
     7081 tggccgcttt tctggattca tcgactgtgg ccggctgggt gtggcggacc gctatcagga
     7141 catagcgttg gctacccgtg atattgctga agagcttggc ggcgaatggg ctgaccgctt
     7201 cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc atcgccttct atcgccttct
     7261 tgacgagttc ttctgaaatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat
     7321 tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca
     7381 tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc
     7441 tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc
     7501 tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt
     7561 cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg
     7621 gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga agctgacgtc
     7681 ctttccatgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta
     7741 cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg
     7801 gcctcttccg cgtctccgcc ttcgccctca gacgagtcgg atctcccttt ggccgcctcc
     7861 ccgcctggta cctttaagac caatgactta caaggcagct gtagatctta gccacttttt
     7921 aaaagaaaag gggggactgg aagggctaat tcactcccaa cgaaaataag atctgctttt
     7981 tgcttgtact gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact
     8041 agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc
     8101 ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa
     8161 aatctctagc agtagtagtt catgtcatct tattattcag tatttataac ttgcaaagaa
     8221 atgaatatca gagagtgaga ggaacttgtt tattgcagct tataatggtt acaaataaag
     8281 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
     8341 gtccaaactc atcaatgtat cttatcatgt ctggctctag ctatcccgcc cctaactccg
     8401 cccagttccg cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc
     8461 gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta
     8521 gacttttgca gagacggccc aaattcgtaa tcatggtcat agctgtttcc tgtgtgaaat
     8581 tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg
     8641 ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag
     8701 tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt
     8761 ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
     8821 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
     8881 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
     8941 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
     9001 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
     9061 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
     9121 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
     9181 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
     9241 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
     9301 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
     9361 ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct
     9421 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
     9481 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
     9541 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
     9601 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
     9661 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
     9721 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
     9781 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
     9841 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag
     9901 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
     9961 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
    10021 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
    10081 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
    10141 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
    10201 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
    10261 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
    10321 tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
    10381 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
    10441 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
    10501 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
    10561 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat
    10621 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
    10681 cgcacatttc cccgaaaagt gccacctgac gtctaagaaa ccattattat catgacatta
    10741 acctataaaa ataggcgtat cacgaggccc tttcgtctcg cgcgtttcgg tgatgacggt
    10801 gaaaacctct gacacatgca gctcccggag acggtcacag cttgtctgta agcggatgcc
    10861 gggagcagac aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg gggctggctt
    10921 aactatgcgg catcagagca gattgtactg agagtgcacc atatgcggtg tgaaataccg
    10981 cacagatgcg taaggagaaa ataccgcatc aggcgccatt cgccattcag gctgcgcaac
    11041 tgttgggaag ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga
    11101 tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa
    11161 acgacggcca gtgccaagct g
//
