LOCUS       V045804                10595 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V045804
VERSION     V045804
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1920
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2010..2213
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     regulatory      2296..2305
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             2317..5319
                     /label="Dhx36(NM_028136)"
                     /note="Dhx36(NM_028136)"
                     /gene="Dhx36"
     promoter        5364..5575
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             5588..5856
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     CDS             5889..6683
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MAEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     misc_feature    6690..7278
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7352..7585
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    7657..7778
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      7797..7932
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(7970..7986)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    7994..8010
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8018..8048)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8063..8084
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8372..8960)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9131..9991)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(9992..10096)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     10570..10586
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt
     1921 atcgatacta gtattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
     1981 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
     2041 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
     2101 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
     2161 tgacgcaaat gggcggtagg cgtgtacggt gggaggttta tataagcaga gctcgtttag
     2221 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagattct
     2281 agagctagcg aattcgccac catgggatcc gccaccatga gctacgacta tcatcagagc
     2341 tggagccgcg atgggggccc acggggctcc ggccagggct ccagcggcgg cggcggcgga
     2401 gggagccggg gctcgggcgg cggcggaggg ggccgcggcg gccggggccg gcatcccgca
     2461 caccttaagg gtcgcgagat cggcctgtgg tacgccaaga agcagacgca gaagaacaag
     2521 gaggctgaga ggcaggagag agctgtagtg cacatggatg aacgtcgaga agagcaaatt
     2581 gtgcagctgc tgaattcagt ccaagctaag actgacaaag attcagaagc gcagatatcc
     2641 tggtttgctc ctgaggatca tgggtatggt actgaagttt cttcagagaa aaaaataaac
     2701 tcagagaaga aacttgacaa ccaggaaaag aaattgctaa accaagaaaa aaagacattt
     2761 aggatcacag acaaatcata tattgaccga gattctgagt atttattgca agaaaatgag
     2821 ccaaacctaa gcttagatca acatttactg gaagatttac aaaggaaaaa aactgaccct
     2881 cgatatatag agatgcagcg tttcagaaaa aagctgcctt catatggaat gcagaaggag
     2941 ctggtaaatc taatcaataa ccatcaggtg acagtaataa gtggtgaaac tggttgtggc
     3001 aaaaccactc aggttacgca gttcatcttg gataactaca tcgaaagagg aaaagggtct
     3061 gcctgcagaa tagtgtgtac tcagccgaga agaattagtg ccatttcagt cgctgagaga
     3121 gtggccacag aaagggcaga gtcttgtggc aatggtaata gtactggata ccagattcgt
     3181 cttcaaagtc ggttgccaag gaaacaaggt tctatcttat actgcacaac aggaatcatt
     3241 cttcagtggc tccagtccga ctcacgtttg tccagtgtta gtcatattgt acttgatgaa
     3301 attcatgaaa ggaatctaca gtcggatgtt ttaatgactg ttattaaaga tcttctccat
     3361 tttcgatctg atctcaaagt aatattgatg agtgcaactt tgaatgctga gaaattttca
     3421 gaatattttg gtaactgtcc aatgatacat atacccgggt ttacttttcc agttgtggaa
     3481 tatcttttgg aagatatcat tgaaaaaata agatatgttc cagaccaaaa agaacataga
     3541 tcccagttca agaggggttt catgcagggt catgtaaata gacaagaaaa agaagaaaaa
     3601 gaggccatct ataaggaacg ctggccagcg tatataaagg aactgcggac aagatactct
     3661 gcaagtaccg tagatgtttt gcaaatgatg gatgatgata aagttgatct gaatttgatt
     3721 gctgccctta ttcgatacat tgttttggaa gaagaggatg gtgcaatatt ggtctttcta
     3781 ccaggctggg acaatatcag tactttgcat gatctcttga tgtcacaagt gatgtttaaa
     3841 tcagataagt ttctcattat acctttacat tcactgatgc ctaccgtcaa ccagacacag
     3901 gtatttaaaa aaactcctcc cggtgttcgg aaaatagtaa ttgctaccaa cattgcagag
     3961 actagcatca ccatagatga tgtggtttat gtaatagatg gaggaaaaat taaagaaaca
     4021 cactttgaca cacagaacaa catcagtacc atgtctgctg agtgggttag taaagctaat
     4081 gccaaacaga ggaaagggag agctggaaga gttcagcctg gtcattgtta tcatctgtat
     4141 aatggtctta gagcaagtct tctagatgac taccaactac cagaaatttt gagaactcct
     4201 ttggaagaac tctgtttgca aataaagatc ttgaggctgg gtggaattgc ttattttctg
     4261 agtagattaa tggatccacc atcaaatgag gcagtggtgc tctccataaa acacctaatg
     4321 gaactgagtg ctttggataa gcaagaagaa ttaacacctc ttggtgtcca tttagcccga
     4381 ctgcctgttg agccacatat tggaaaaatg attctctttg gagctttgtt ctgctgctta
     4441 gatccagttc tcaccattgc tgccagtctc agctttaaag atccctttgt cattccattg
     4501 ggaaaagaaa agattgcaga tgcaagaaga aaagaactgg caaaggaaac tagaagtgat
     4561 cacctgacag ttgtgaatgc atttgagggc tgggaagaag ccaaacgacg tggtttcagg
     4621 tatgaaaagg actactgctg ggaatatttt ctgtcttcga acacactaca gatgctgcat
     4681 aacatgaagg gacagtttgc tgagcatctt cttggagctg gatttgtaag cagtagaagt
     4741 cccaaagatc caaaagctaa tataaattca gataatgaga agataattaa agctgtcatc
     4801 tgtgctggtt tatatcccaa agttgctaaa atccgactaa atttgggtaa aaagaggaaa
     4861 atggtaaaag ttcacacgaa gtctgacggc ctggtttcta ttcatcctaa gtctgtcaac
     4921 gtggagcaga cagacttcca ctacaactgg cttatctatc acctgaagat gagaacaagc
     4981 agtatatact tgtacgactg tacagaagtg tcaccatact gcctcctgtt ctttggagga
     5041 gatatttcca tccagaaaga taaggatcag gaaattattg ctgtagatga gtggattgtg
     5101 tttcagtctc cagaaagaat tgcccatctt gttaagggac taagaaagga actggatagc
     5161 cttctacaag agaagattga aagccctcat cctgtagact gggacgacac taagtcaaga
     5221 gactgtgcag tactgtcagc tattctagac ttgatcaaaa ctcaagaaaa ggctactcca
     5281 aggaacttgc caccacggtc acaggacgga tattatagct gagcggccgc gaaggatctg
     5341 cgatcgctcc ggtgcccgtc agtgggcaga gcgcacatcg cccacagtcc ccgagaagtt
     5401 ggggggaggg gtcggcaatt gaacgggtgc ctagagaagg tggcgcgggg taaactggga
     5461 aagtgatgtc gtgtactggc tccgcctttt tcccgagggt gggggagaac cgtatataag
     5521 tgcagtagtc gccgtgaacg ttctttttcg caacgggttt gccgccagaa cacagctgaa
     5581 gcttcgaggg gctcgcatct ctccttcacg cgcccgccgc cctacctgag gccgccatcc
     5641 acgccggttg agtcgcgttc tgccgcctcc cgcctgtggt gcctcctgaa ctgcgtccgc
     5701 cgtctaggta agtttaaagc tcaggtcgag accgggcctt tgtccggcgc tcccttggag
     5761 cctacctaga ctcagccggc tctccacgct ttgcctgacc ctgcttgctc aactctacgt
     5821 ctttgtttcg ttttctgttc tgcgccgtta cagatccaag ctgtgaccgg cgcctacgct
     5881 aggccaccat ggctgaacaa gatggattgc acgcaggttc tccggccgct tgggtggaga
     5941 ggctattcgg ctatgactgg gcacaacaga caatcggctg ctctgatgcc gccgtgttcc
     6001 ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga
     6061 atgaactgca ggacgaggca gcgcggctat cgtggctggc cacgacgggc gttccttgcg
     6121 cagctgtgct cgacgttgtc actgaagcgg gaagggactg gctgctattg ggcgaagtgc
     6181 cggggcagga tctcctgtca tctcaccttg ctcctgccga gaaagtatcc atcatggctg
     6241 atgcaatgcg gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga
     6301 aacatcgcat cgagcgagca cgtactcgga tggaagccgg tcttgtcgat caggatgatc
     6361 tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc aaggcgcgca
     6421 tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg
     6481 tggaaaatgg ccgcttttct ggattcatcg actgtggccg gctgggtgtg gcggaccgct
     6541 atcaggacat agcgttggct acccgtgata ttgctgaaga gcttggcggc gaatgggctg
     6601 accgcttcct cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc gccttctatc
     6661 gccttcttga cgagttcttc tgagtcgaca atcaacctct ggattacaaa atttgtgaaa
     6721 gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa
     6781 tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat
     6841 cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt
     6901 gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc
     6961 tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc
     7021 ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg
     7081 ggaaatcatc gtcctttcct tggctgctcg cctgtgttgc cacctggatt ctgcgcggga
     7141 cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc
     7201 tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc
     7261 tttgggccgc ctccccgcct ggtaccttta agaccaatga cttacaaggc agctgtagat
     7321 cttagccact ttttaaaaga aaagggggga ctggaagggc taattcactc ccaacgaaaa
     7381 taagatctgc tttttgcttg tactgggtct ctctggttag accagatctg agcctgggag
     7441 ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt
     7501 caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt
     7561 tagtcagtgt ggaaaatctc tagcagtagt agttcatgtc atcttattat tcagtattta
     7621 taacttgcaa agaaatgaat atcagagagt gagaggaact tgtttattgc agcttataat
     7681 ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat
     7741 tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggct ctagctatcc
     7801 cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta
     7861 tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct
     7921 tttttggagg cctagacttt tgcagagacg gcccaaattc gtaatcatgg tcatagctgt
     7981 ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa
     8041 agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac
     8101 tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg
     8161 cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc
     8221 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
     8281 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
     8341 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
     8401 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
     8461 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
     8521 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
     8581 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
     8641 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
     8701 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
     8761 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat
     8821 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
     8881 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
     8941 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
     9001 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct
     9061 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt
     9121 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
     9181 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac
     9241 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat
     9301 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
     9361 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
     9421 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta
     9481 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt
     9541 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag
     9601 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa
     9661 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc
     9721 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt
     9781 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc
     9841 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta
     9901 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa
     9961 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca
    10021 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
    10081 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa gaaaccatta
    10141 ttatcatgac attaacctat aaaaataggc gtatcacgag gccctttcgt ctcgcgcgtt
    10201 tcggtgatga cggtgaaaac ctctgacaca tgcagctccc ggagacggtc acagcttgtc
    10261 tgtaagcgga tgccgggagc agacaagccc gtcagggcgc gtcagcgggt gttggcgggt
    10321 gtcggggctg gcttaactat gcggcatcag agcagattgt actgagagtg caccatatgc
    10381 ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg catcaggcgc cattcgccat
    10441 tcaggctgcg caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc
    10501 tggcgaaagg gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt
    10561 cacgacgttg taaaacgacg gccagtgcca agctg
//
