LOCUS       V037645                10376 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V037645
VERSION     V037645
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1920
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2010..2213
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     regulatory      2288..2297
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             2297..2362
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     CDS             2369..4342
                     /label="ENG(NM_001114753)"
                     /note="ENG(NM_001114753)"
                     /gene="ENG"
     CDS             4385..5104
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /transl_table=1
                     /translation="M,V,SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKL
                     TLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNG
                     IKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMV
                     LLEFVTAAGITLGMDELYK*"
                     /label="EGFP"
     promoter        5145..5356
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             5369..5637
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     CDS             5670..6464
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MAEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     misc_feature    6471..7059
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7133..7366
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    7438..7559
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      7578..7713
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(7751..7767)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    7775..7791
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(7799..7829)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    7844..7865
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8153..8741)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(8912..9772)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(9773..9877)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     10351..10367
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt
     1921 atcgatacta gtattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
     1981 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
     2041 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
     2101 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
     2161 tgacgcaaat gggcggtagg cgtgtacggt gggaggttta tataagcaga gctcgtttag
     2221 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagattct
     2281 agagctagcc accatggact acaaagacca tgacggtgat tataaagatc atgacatcga
     2341 ctacaaggat gacgatgaca aggaattcat ggaccgcggc acgctccctc tggctgttgc
     2401 cctgctgctg gccagctgca gcctcagccc cacaagtctt gcagaaacag tccattgtga
     2461 ccttcagcct gtgggccccg agaggggcga ggtgacatat accactagcc aggtctcgaa
     2521 gggctgcgtg gctcaggccc ccaatgccat ccttgaagtc catgtcctct tcctggagtt
     2581 cccaacgggc ccgtcacagc tggagctgac tctccaggca tccaagcaaa atggcacctg
     2641 gccccgagag gtgcttctgg tcctcagtgt aaacagcagt gtcttcctgc atctccaggc
     2701 cctgggaatc ccactgcact tggcctacaa ttccagcctg gtcaccttcc aagagccccc
     2761 gggggtcaac accacagagc tgccatcctt ccccaagacc cagatccttg agtgggcagc
     2821 tgagaggggc cccatcacct ctgctgctga gctgaatgac ccccagagca tcctcctccg
     2881 actgggccaa gcccaggggt cactgtcctt ctgcatgctg gaagccagcc aggacatggg
     2941 ccgcacgctc gagtggcggc cgcgtactcc agccttggtc cggggctgcc acttggaagg
     3001 cgtggccggc cacaaggagg cgcacatcct gagggtcctg ccgggccact cggccgggcc
     3061 ccggacggtg acggtgaagg tggaactgag ctgcgcaccc ggggatctcg atgccgtcct
     3121 catcctgcag ggtcccccct acgtgtcctg gctcatcgac gccaaccaca acatgcagat
     3181 ctggaccact ggagaatact ccttcaagat ctttccagag aaaaacattc gtggcttcaa
     3241 gctcccagac acacctcaag gcctcctggg ggaggcccgg atgctcaatg ccagcattgt
     3301 ggcatccttc gtggagctac cgctggccag cattgtctca cttcatgcct ccagctgcgg
     3361 tggtaggctg cagacctcac ccgcaccgat ccagaccact cctcccaagg acacttgtag
     3421 cccggagctg ctcatgtcct tgatccagac aaagtgtgcc gacgacgcca tgaccctggt
     3481 actaaagaaa gagcttgttg cgcatttgaa gtgcaccatc acgggcctga ccttctggga
     3541 ccccagctgt gaggcagagg acaggggtga caagtttgtc ttgcgcagtg cttactccag
     3601 ctgtggcatg caggtgtcag caagtatgat cagcaatgag gcggtggtca atatcctgtc
     3661 gagctcatca ccacagcgga aaaaggtgca ctgcctcaac atggacagcc tctctttcca
     3721 gctgggcctc tacctcagcc cacacttcct ccaggcctcc aacaccatcg agccggggca
     3781 gcagagcttt gtgcaggtca gagtgtcccc atccgtctcc gagttcctgc tccagttaga
     3841 cagctgccac ctggacttgg ggcctgaggg aggcaccgtg gaactcatcc agggccgggc
     3901 ggccaagggc aactgtgtga gcctgctgtc cccaagcccc gagggtgacc cgcgcttcag
     3961 cttcctcctc cacttctaca cagtacccat acccaaaacc ggcaccctca gctgcacggt
     4021 agccctgcgt cccaagaccg ggtctcaaga ccaggaagtc cataggactg tcttcatgcg
     4081 cttgaacatc atcagccctg acctgtctgg ttgcacaagc aaaggcctcg tcctgcccgc
     4141 cgtgctgggc atcacctttg gtgccttcct catcggggcc ctgctcactg ctgcactctg
     4201 gtacatctac tcgcacacgc gttcccccag caagcgggag cccgtggtgg cggtggctgc
     4261 cccggcctcc tcggagagca gcagcaccaa ccacagcatc gggagcaccc agagcacccc
     4321 ctgctccacc agcagcatgg caggcggtgg aggaagcggc ggaggtggat ctggatccgc
     4381 caccatggtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct
     4441 ggacggcgac gtaaacggcc acaagttcag cgtgtccggc gagggcgagg gcgatgccac
     4501 ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc
     4561 caccctcgtg accaccctga cctacggcgt gcagtgcttc agccgctacc ccgaccacat
     4621 gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat
     4681 cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac
     4741 cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg
     4801 gcacaagctg gagtacaact acaacagcca caacgtctat atcatggccg acaagcagaa
     4861 gaacggcatc aaggtgaact tcaagatccg ccacaacatc gaggacggca gcgtgcagct
     4921 cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa
     4981 ccactacctg agcacccagt ccgccctgag caaagacccc aacgagaagc gcgatcacat
     5041 ggtcctgctg gagttcgtga ccgccgccgg gatcactctc ggcatggacg agctgtacaa
     5101 gtagcggccg cgaaggatct gcgatcgctc cggtgcccgt cagtgggcag agcgcacatc
     5161 gcccacagtc cccgagaagt tggggggagg ggtcggcaat tgaacgggtg cctagagaag
     5221 gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg ctccgccttt ttcccgaggg
     5281 tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac gttctttttc gcaacgggtt
     5341 tgccgccaga acacagctga agcttcgagg ggctcgcatc tctccttcac gcgcccgccg
     5401 ccctacctga ggccgccatc cacgccggtt gagtcgcgtt ctgccgcctc ccgcctgtgg
     5461 tgcctcctga actgcgtccg ccgtctaggt aagtttaaag ctcaggtcga gaccgggcct
     5521 ttgtccggcg ctcccttgga gcctacctag actcagccgg ctctccacgc tttgcctgac
     5581 cctgcttgct caactctacg tctttgtttc gttttctgtt ctgcgccgtt acagatccaa
     5641 gctgtgaccg gcgcctacgc taggccacca tggctgaaca agatggattg cacgcaggtt
     5701 ctccggccgc ttgggtggag aggctattcg gctatgactg ggcacaacag acaatcggct
     5761 gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg cccggttctt tttgtcaaga
     5821 ccgacctgtc cggtgccctg aatgaactgc aggacgaggc agcgcggcta tcgtggctgg
     5881 ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg ggaagggact
     5941 ggctgctatt gggcgaagtg ccggggcagg atctcctgtc atctcacctt gctcctgccg
     6001 agaaagtatc catcatggct gatgcaatgc ggcggctgca tacgcttgat ccggctacct
     6061 gcccattcga ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg atggaagccg
     6121 gtcttgtcga tcaggatgat ctggacgaag agcatcaggg gctcgcgcca gccgaactgt
     6181 tcgccaggct caaggcgcgc atgcccgacg gcgaggatct cgtcgtgacc catggcgatg
     6241 cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc tggattcatc gactgtggcc
     6301 ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc tacccgtgat attgctgaag
     6361 agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt
     6421 cgcagcgcat cgccttctat cgccttcttg acgagttctt ctgagtcgac aatcaacctc
     6481 tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc
     6541 tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca
     6601 ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg
     6661 tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca
     6721 ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg
     6781 cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg
     6841 acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg
     6901 ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg
     6961 accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc
     7021 ctcagacgag tcggatctcc ctttgggccg cctccccgcc tggtaccttt aagaccaatg
     7081 acttacaagg cagctgtaga tcttagccac tttttaaaag aaaagggggg actggaaggg
     7141 ctaattcact cccaacgaaa ataagatctg ctttttgctt gtactgggtc tctctggtta
     7201 gaccagatct gagcctggga gctctctggc taactaggga acccactgct taagcctcaa
     7261 taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac
     7321 tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtag tagttcatgt
     7381 catcttatta ttcagtattt ataacttgca aagaaatgaa tatcagagag tgagaggaac
     7441 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat
     7501 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat
     7561 catgtctggc tctagctatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc
     7621 atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat
     7681 tccagaagta gtgaggaggc ttttttggag gcctagactt ttgcagagac ggcccaaatt
     7741 cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca
     7801 acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca
     7861 cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc
     7921 attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt
     7981 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
     8041 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
     8101 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
     8161 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
     8221 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
     8281 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
     8341 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
     8401 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
     8461 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
     8521 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
     8581 gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
     8641 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
     8701 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
     8761 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
     8821 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
     8881 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta
     8941 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa
     9001 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac
     9061 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa
     9121 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
     9181 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg
     9241 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag
     9301 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg
     9361 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc
     9421 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat
     9481 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata
     9541 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa
     9601 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca
     9661 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
     9721 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
     9781 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
     9841 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
     9901 ctgacgtcta agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga
     9961 ggccctttcg tctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac atgcagctcc
    10021 cggagacggt cacagcttgt ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg
    10081 cgtcagcggg tgttggcggg tgtcggggct ggcttaacta tgcggcatca gagcagattg
    10141 tactgagagt gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc
    10201 gcatcaggcg ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg
    10261 cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg
    10321 taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc aagctg
//
