LOCUS       V018555                 9950 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V018555
VERSION     V018555
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1920
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2010..2213
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2302..4674
                     /label="H6PD(NM_004285)"
                     /note="H6PD(NM_004285)"
                     /gene="H6PD"
     promoter        4719..4930
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             4943..5211
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     CDS             5244..6038
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MAEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     misc_feature    6045..6633
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             6707..6940
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    7012..7133
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      7152..7287
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(7325..7341)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    7349..7365
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(7373..7403)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    7418..7439
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(7727..8315)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(8486..9346)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(9347..9451)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     9925..9941
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt
     1921 atcgatacta gtattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
     1981 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
     2041 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
     2101 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
     2161 tgacgcaaat gggcggtagg cgtgtacggt gggaggttta tataagcaga gctcgtttag
     2221 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagattct
     2281 agagctagcg aattcgccac catgtggaat atgctcatag tggcgatgtg cttggccctt
     2341 ctgggctgcc tgcaagccca ggagctccag ggacatgtct ccataatcct gctgggagca
     2401 actggggacc tggctaagaa gtacttatgg cagggactgt tccagctgta cctggatgaa
     2461 gcggggaggg gtcacagttt tagcttccat ggagctgctc tgacagcccc caagcagggt
     2521 caagagctca tggccaaggc cctggaatcc ctctcctgcc ccaaggacat ggcacccagt
     2581 cactgtgcag agcacaagga tcagttcctg cagctgagcc agtaccgcca actgaagacg
     2641 gccgaggact atcaggccct gaacaaggac atcgaggcac agctccagca cgcaggcctc
     2701 cgggaggctg gcaggatctt ctacttctca gtgccaccct tcgcctatga agacattgcc
     2761 cgcaacatca acagtagctg ccggccaggc ccgggcgcct ggctgcgggt tgtccttgag
     2821 aaaccctttg gccatgacca cttctcagcc cagcagctgg ccacagaact cgggaccttt
     2881 ttccaggagg aggagatgta ccgggtggac cattacttag gcaagcaggc tgtggcgcag
     2941 atcctgcctt tccgagacca gaaccgcaag gctttggacg gcctctggaa ccggcaccat
     3001 gtggagcggg tggagatcat catgaaagag accgtggatg ctgaaggccg caccagcttc
     3061 tatgaggagt acggtgtcat tcgcgacgtc ctccagaacc atctgacgga ggtcctcacc
     3121 ctcgtggcca tggagctgcc ccacaatgtc agcagtgcgg aggctgtgct gcggcacaag
     3181 cttcaggtct tccaggcgct gcggggcctg cagaggggca gtgccgtcgt gggccagtac
     3241 cagtcttaca gtgagcaggt gcgcagagag ctgcagaagc cagacagctt ccacagcctg
     3301 acgccgacct tcgcagccgt cctagtgcac attgacaacc ttcgctggga gggcgtgcct
     3361 ttcatcctga tgtctggcaa agccttggac gagagagtgg gctacgctcg gatcttgttc
     3421 aagaaccagg cctgctgtgt gcagagcgaa aagcactggg ccgcggcgca gagccagtgc
     3481 ctgccccggc agctcgtctt ccacatcggc catggcgacc tgggcagccc tgccgtgctg
     3541 gtcagcagga acctgttcag gccctccctg ccctccagct ggaaggaaat ggagggacca
     3601 cctgggctcc gccttttcgg cagccctctg tccgattact acgcctacag ccctgtgcgg
     3661 gagcgggacg cccactccgt cctcttatcc catatcttcc atggccggaa gaatttcttc
     3721 atcaccacag agaacttgct ggcctcctgg aacttctgga cccctctgct ggagagcctg
     3781 gcccataagg ccccacgcct ctaccctgga ggagctgaga atggccgtct gttggacttt
     3841 gagttcagta gcggccggtt gttcttttcc cagcagcagc cggagcagct ggtgccaggg
     3901 ccagggccgg ccccaatgcc cagtgacttc caggtcctca gggccaagta ccgagagagc
     3961 ccgctggtct ccgcctggtc cgaggagctg atctctaagc tggctaatga catcgaggcc
     4021 accgctgtgc gagccgtgcg gcgctttggc cagttccacc tggcactgtc ggggggctcg
     4081 agccccgtgg ccctgttcca gcagctggcc acggcgcact atggcttccc ctgggcccac
     4141 acgcacctgt ggctggttga cgagcgctgc gtcccactct cagacccgga gtccaacttc
     4201 cagggcctgc aggcccacct gctgcagcac gtccggatcc cctactacaa catccacccc
     4261 atgcctgtgc acctgcagca gcggctctgc gccgaggagg accagggcgc ccagatctat
     4321 gccagggaga tctcagccct ggtggccaac agcagcttcg acctggtgct gctgggcatg
     4381 ggtgccgacg ggcacacagc ctccctcttc ccacagtcac ccactggcct ggatggcgag
     4441 cagctggtcg tgctgaccac gagcccctcc cagccacacc gccgcatgag ccttagcctg
     4501 cctctcatca accgcgccaa gaaggtggca gtcctggtca tgggcaggat gaagcgtgag
     4561 atcaccacgc tggtgagccg ggtgggccat gagcccaaga agtggcccat ctcgggtgtc
     4621 ctgccgcact ccggccagct ggtgtggtac atggactacg acgccttcct gggatgagcg
     4681 gccgcgaagg atctgcgatc gctccggtgc ccgtcagtgg gcagagcgca catcgcccac
     4741 agtccccgag aagttggggg gaggggtcgg caattgaacg ggtgcctaga gaaggtggcg
     4801 cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg agggtggggg
     4861 agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg ggtttgccgc
     4921 cagaacacag ctgaagcttc gaggggctcg catctctcct tcacgcgccc gccgccctac
     4981 ctgaggccgc catccacgcc ggttgagtcg cgttctgccg cctcccgcct gtggtgcctc
     5041 ctgaactgcg tccgccgtct aggtaagttt aaagctcagg tcgagaccgg gcctttgtcc
     5101 ggcgctccct tggagcctac ctagactcag ccggctctcc acgctttgcc tgaccctgct
     5161 tgctcaactc tacgtctttg tttcgttttc tgttctgcgc cgttacagat ccaagctgtg
     5221 accggcgcct acgctaggcc accatggctg aacaagatgg attgcacgca ggttctccgg
     5281 ccgcttgggt ggagaggcta ttcggctatg actgggcaca acagacaatc ggctgctctg
     5341 atgccgccgt gttccggctg tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc
     5401 tgtccggtgc cctgaatgaa ctgcaggacg aggcagcgcg gctatcgtgg ctggccacga
     5461 cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg gactggctgc
     5521 tattgggcga agtgccgggg caggatctcc tgtcatctca ccttgctcct gccgagaaag
     5581 tatccatcat ggctgatgca atgcggcggc tgcatacgct tgatccggct acctgcccat
     5641 tcgaccacca agcgaaacat cgcatcgagc gagcacgtac tcggatggaa gccggtcttg
     5701 tcgatcagga tgatctggac gaagagcatc aggggctcgc gccagccgaa ctgttcgcca
     5761 ggctcaaggc gcgcatgccc gacggcgagg atctcgtcgt gacccatggc gatgcctgct
     5821 tgccgaatat catggtggaa aatggccgct tttctggatt catcgactgt ggccggctgg
     5881 gtgtggcgga ccgctatcag gacatagcgt tggctacccg tgatattgct gaagagcttg
     5941 gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc gattcgcagc
     6001 gcatcgcctt ctatcgcctt cttgacgagt tcttctgagt cgacaatcaa cctctggatt
     6061 acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg
     6121 gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct
     6181 cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc
     6241 aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca
     6301 ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac
     6361 tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt
     6421 ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct gctcgcctgt gttgccacct
     6481 ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc
     6541 cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga
     6601 cgagtcggat ctccctttgg gccgcctccc cgcctggtac ctttaagacc aatgacttac
     6661 aaggcagctg tagatcttag ccacttttta aaagaaaagg ggggactgga agggctaatt
     6721 cactcccaac gaaaataaga tctgcttttt gcttgtactg ggtctctctg gttagaccag
     6781 atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc
     6841 ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga
     6901 tccctcagac ccttttagtc agtgtggaaa atctctagca gtagtagttc atgtcatctt
     6961 attattcagt atttataact tgcaaagaaa tgaatatcag agagtgagag gaacttgttt
     7021 attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca
     7081 tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc
     7141 tggctctagc tatcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct
     7201 gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga
     7261 agtagtgagg aggctttttt ggaggcctag acttttgcag agacggccca aattcgtaat
     7321 catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac
     7381 gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa
     7441 ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat
     7501 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc
     7561 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
     7621 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
     7681 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
     7741 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
     7801 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
     7861 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
     7921 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
     7981 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
     8041 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
     8101 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
     8161 ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
     8221 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
     8281 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
     8341 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
     8401 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
     8461 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
     8521 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
     8581 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac
     8641 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
     8701 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
     8761 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac
     8821 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
     8881 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
     8941 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
     9001 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
     9061 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc
     9121 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
     9181 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
     9241 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
     9301 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc
     9361 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
     9421 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg
     9481 tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct
     9541 ttcgtctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga
     9601 cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag
     9661 cgggtgttgg cgggtgtcgg ggctggctta actatgcggc atcagagcag attgtactga
     9721 gagtgcacca tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca
     9781 ggcgccattc gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt
     9841 cgctattacg ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc
     9901 cagggttttc ccagtcacga cgttgtaaaa cgacggccag tgccaagctg
//
