LOCUS       V042558                11477 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V042558
VERSION     V042558
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1920
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2010..2213
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2302..5433
                     /label="Itgav(NM_008402)"
                     /note="Itgav(NM_008402)"
                     /gene="Itgav"
     CDS             5479..6198
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="enhanced YFP"
                     /transl_table=1
                     /translation="M,V,SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKL
                     TLKFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNG
                     IKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMV
                     LLEFVTAAGITLGMDELYK*"
                     /label="EYFP"
     promoter        6246..6457
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             6470..6738
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     CDS             6771..7565
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MAEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     misc_feature    7572..8160
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             8234..8467
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    8539..8660
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      8679..8814
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(8852..8868)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8876..8892
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8900..8930)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8945..8966
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(9254..9842)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(10013..10873)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10874..10978)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     11452..11468
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt
     1921 atcgatacta gtattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
     1981 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
     2041 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
     2101 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
     2161 tgacgcaaat gggcggtagg cgtgtacggt gggaggttta tataagcaga gctcgtttag
     2221 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagattct
     2281 agagctagcg aattcgccac catggctgct cccgggcgcc tgctgctacg ccctcgcccc
     2341 ggcggcctcc tgctgctgct gccgggtctc ctgctgcccc tcgccgacgc cttcaacctg
     2401 gacgtcgaaa gtcccgccga gtatgcgggt cccgagggaa gttacttcgg attcgccgtg
     2461 gacttcttcg agcccagcac gtcctccagg atgtttctcc tggtgggagc ccccaaagcg
     2521 aacacgaccc agcctgggat tgtagaagga gggcaagttc tcaaatgtga atgctcatcc
     2581 agccgccggt gccagcccat tgagtttgat tcaacaggca atcgagatta tgccaaagat
     2641 gacccactgg agtttaagtc ccaccagtgg tttggagcct ctgtgaggtc aaagcaggat
     2701 aaaatcttgg cctgtgctcc attgtaccac tggaggactg agatgaagca ggagagagag
     2761 ccagttggaa cctgctttct tcaggacggc acaaagaccg ttgagtatgc tccatgcagg
     2821 tcaaaaaata ttgatgctga tggccaggga ttttgtcaag gaggattcag cattgatttt
     2881 actaaagctg acagagtact tctcggtggt cctggtagct tttattggca aggtcagctc
     2941 atttcggacc aagtggcaga aatcatatct aaatatgacc caaatgtcta cagcatcaaa
     3001 tataataacc aattagcaac acggactgca caagcaattt ttgatgacag ttatttgggt
     3061 tactctgtgg ccgtgggaga cttcaatggt gacggcattg aagattttgt ctcaggagtt
     3121 ccaagagcag caaggacttt gggaatggtt tatatttatg atgggaaaaa tatgtcctct
     3181 ttacacaatt ttactggtga acagatggct gcgtattttg gattttctgt agctgctact
     3241 gacattaatg gggatgatta cgcagatgtg tttattggag cccccctgtt catggaccga
     3301 ggttccgatg ggaaactcca ggaggttggc caggtctcag tgtctctgca gagagcagtg
     3361 ggagacttcc agactacaaa gctgaacggc tttgaggttt ttgccaggtt tggaagtgcc
     3421 atagctccat tgggagacct ggaccaggat ggcttcaatg atattgcaat tgctgctccc
     3481 tatggtggtg aagataagaa gggacttgtt tatatcttca atggaaggtc cacaggcttg
     3541 aattcggtgc catctcaaat cctcgagggg cagtgggctg ctcagagcat gcccccaagc
     3601 tttggctatt caatgaaggg agctacagat gtagacagga atggatatcc agacttagtt
     3661 gtaggagctt ttggtgtgga tcgagctgtc ttatacagag ccagacccgt tgtcactgta
     3721 aatgctggcc ttgaagtgta ccctagcatt ttaaatcaag acaataagat ctgcccgttg
     3781 cctgggacag ctctcaaggt ttcctgtttt aatgtcagat tctgcttaaa ggcagatggc
     3841 aagggaaccc ttcctcggaa gctccacttc caggtggagc ttctactgga taaactcaag
     3901 cagaagggag ccatccgacg agcactgttt ctccataaca ggtccccggt ccactccaag
     3961 accatgactg ttttcagggg gggacagatg cagtgtgagg aactggtcgc ctatcttcgg
     4021 gatgaatctg aatttagaga caagctcact cccatcacca ttttcatgga gtatcggttg
     4081 gaccagagaa cggctgctga tgccacaggc ttgcagccca tcctgaacca gttcactccg
     4141 gccaacgtca gtcggcaggc tcatattcta cttgactgtg gtgaagacaa tgtctgtaaa
     4201 cctaaattgg aagtttctgt aaatagtgat caaaagaaga tctatattgg ggacgacaac
     4261 cctctgacac tgactgtgaa ggcgcagaat caaggggaag gcgcctatga agctgagctc
     4321 atcgtttcta tcccaccgca ggctgacttc atcggggttg tccggaacaa cgaagcctta
     4381 gcaagactgt cctgtgcatt taagacagaa aaccaaactc ggcaggtggt gtgcgacctt
     4441 ggaaacccga tgaaggctgg aactcaactg ctcgctggtc ttcgtttcag cgtgcaccag
     4501 cagtcagaga tggatacttc tgtgaagttt gacttgaaaa tccagagctc taattctttt
     4561 gacaatgtaa gtccagttgt gtcttacaag gttgacctcg ccgttttggc tgctgtggag
     4621 ataagaggag tttcaagtcc agatcatatc tttcttccaa ttccaaattg ggagtacaag
     4681 gaaaaccctg agactgaaga agacgttggg cctattgttc agcacatata tgagctgaga
     4741 aacaatggtc caagttcatt cagcaaggca attctaaatc tccagtggcc ttacaaatac
     4801 aacaacaaca ctctgttgta cattcttcat tacgacattg acgggccaat gaactgcacg
     4861 gcagatacag agatcaaccc tttgagaatc aagacacccg aaaagaatga cacagcggcg
     4921 gcgggacagg gagaaaggaa tcatctcatc accaagcggg atctcaccct cagagaggga
     4981 gatgttcaca ctttgggctg tggaatcgcc aagtgcttgc agatcacctg ccaggttggt
     5041 cgactggata gaggcaagag cgcaatcctg tacgtgaaat ccctgctgtg gaccgagacg
     5101 ttcatgaaca aggagaacca gaaccattcc tattcgctga agtcatctgc ttcctttaat
     5161 atcatagaat tcccttacaa gaacctgccg atcgaggatc tcttcaactc tacactagtc
     5221 accactaaca tcacctgggg cattcagccg gcgcccatgc ctgtgcctgt gtgggtgatc
     5281 atcttggcag ttctcgcagg gctgctgcta ctggctgtgt tggtatttgt aatgtacagg
     5341 atgggctttt tcaaacgtgt ccgaccacct caagaagagc aagaaagaga acagcttcag
     5401 cctcatgaga atggcgaagg aaactctgaa accggcggag gtggaagcgg tggaggcggt
     5461 agtggtggcg gtggaagcat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc
     5521 atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc
     5581 gagggcgatg ccacctacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg
     5641 cccgtgccct ggcccaccct cgtgaccacc ttcggctacg gcctgcagtg cttcgcccgc
     5701 taccccgacc acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacgtc
     5761 caggagcgca ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag
     5821 ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac
     5881 ggcaacatcc tggggcacaa gctggagtac aactacaaca gccacaacgt ctatatcatg
     5941 gccgacaagc agaagaacgg catcaaggtg aacttcaaga tccgccacaa catcgaggac
     6001 ggcagcgtgc agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg
     6061 ctgctgcccg acaaccacta cctgagctac cagtccgccc tgagcaaaga ccccaacgag
     6121 aagcgcgatc acatggtcct gctggagttc gtgaccgccg ccgggatcac tctcggcatg
     6181 gacgagctgt acaagtaagg atccgcggcc gcgaaggatc tgcgatcgct ccggtgcccg
     6241 tcagtgggca gagcgcacat cgcccacagt ccccgagaag ttggggggag gggtcggcaa
     6301 ttgaacgggt gcctagagaa ggtggcgcgg ggtaaactgg gaaagtgatg tcgtgtactg
     6361 gctccgcctt tttcccgagg gtgggggaga accgtatata agtgcagtag tcgccgtgaa
     6421 cgttcttttt cgcaacgggt ttgccgccag aacacagctg aagcttcgag gggctcgcat
     6481 ctctccttca cgcgcccgcc gccctacctg aggccgccat ccacgccggt tgagtcgcgt
     6541 tctgccgcct cccgcctgtg gtgcctcctg aactgcgtcc gccgtctagg taagtttaaa
     6601 gctcaggtcg agaccgggcc tttgtccggc gctcccttgg agcctaccta gactcagccg
     6661 gctctccacg ctttgcctga ccctgcttgc tcaactctac gtctttgttt cgttttctgt
     6721 tctgcgccgt tacagatcca agctgtgacc ggcgcctacg ctaggccacc atggctgaac
     6781 aagatggatt gcacgcaggt tctccggccg cttgggtgga gaggctattc ggctatgact
     6841 gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc
     6901 gcccggttct ttttgtcaag accgacctgt ccggtgccct gaatgaactg caggacgagg
     6961 cagcgcggct atcgtggctg gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg
     7021 tcactgaagc gggaagggac tggctgctat tgggcgaagt gccggggcag gatctcctgt
     7081 catctcacct tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg cggcggctgc
     7141 atacgcttga tccggctacc tgcccattcg accaccaagc gaaacatcgc atcgagcgag
     7201 cacgtactcg gatggaagcc ggtcttgtcg atcaggatga tctggacgaa gagcatcagg
     7261 ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgcg catgcccgac ggcgaggatc
     7321 tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt
     7381 ctggattcat cgactgtggc cggctgggtg tggcggaccg ctatcaggac atagcgttgg
     7441 ctacccgtga tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt
     7501 acggtatcgc cgctcccgat tcgcagcgca tcgccttcta tcgccttctt gacgagttct
     7561 tctgagtcga caatcaacct ctggattaca aaatttgtga aagattgact ggtattctta
     7621 actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta
     7681 ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt
     7741 atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg
     7801 caacccccac tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt
     7861 tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag
     7921 gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaaatca tcgtcctttc
     7981 cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc
     8041 cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc
     8101 ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc
     8161 ctggtacctt taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa
     8221 gaaaaggggg gactggaagg gctaattcac tcccaacgaa aataagatct gctttttgct
     8281 tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg
     8341 aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt
     8401 ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc
     8461 tctagcagta gtagttcatg tcatcttatt attcagtatt tataacttgc aaagaaatga
     8521 atatcagaga gtgagaggaa cttgtttatt gcagcttata atggttacaa ataaagcaat
     8581 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc
     8641 aaactcatca atgtatctta tcatgtctgg ctctagctat cccgccccta actccgccca
     8701 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     8761 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctagact
     8821 tttgcagaga cggcccaaat tcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt
     8881 atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg
     8941 cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg
     9001 gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc
     9061 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
     9121 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
     9181 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     9241 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     9301 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     9361 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     9421 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
     9481 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     9541 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     9601 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     9661 tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc
     9721 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     9781 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     9841 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     9901 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
     9961 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
    10021 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
    10081 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
    10141 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
    10201 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
    10261 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
    10321 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
    10381 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
    10441 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
    10501 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
    10561 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
    10621 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
    10681 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
    10741 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
    10801 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
    10861 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
    10921 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
    10981 catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct
    11041 ataaaaatag gcgtatcacg aggccctttc gtctcgcgcg tttcggtgat gacggtgaaa
    11101 acctctgaca catgcagctc ccggagacgg tcacagcttg tctgtaagcg gatgccggga
    11161 gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc tggcttaact
    11221 atgcggcatc agagcagatt gtactgagag tgcaccatat gcggtgtgaa ataccgcaca
    11281 gatgcgtaag gagaaaatac cgcatcaggc gccattcgcc attcaggctg cgcaactgtt
    11341 gggaagggcg atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa gggggatgtg
    11401 ctgcaaggcg attaagttgg gtaacgccag ggttttccca gtcacgacgt tgtaaaacga
    11461 cggccagtgc caagctg
//
