LOCUS       V036097                11372 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V036097
VERSION     V036097
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1920
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2010..2213
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2302..6090
                     /label="Vars1(NM_011690)"
                     /note="Vars1(NM_011690)"
                     /gene="Vars1"
     promoter        6141..6352
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             6365..6633
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     CDS             6666..7460
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MAEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     misc_feature    7467..8055
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             8129..8362
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    8434..8555
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      8574..8709
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(8747..8763)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8771..8787
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8795..8825)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8840..8861
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(9149..9737)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9908..10768)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10769..10873)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     11347..11363
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt
     1921 atcgatacta gtattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
     1981 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
     2041 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
     2101 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
     2161 tgacgcaaat gggcggtagg cgtgtacggt gggaggttta tataagcaga gctcgtttag
     2221 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagattct
     2281 agagctagcg aattcgccac catgtccatc ctctacgtct ctcctcaccc cgacgccttc
     2341 cccagcctcc gagccctcat cgctgcccga tacggggaag ctggtgacgg tcccgggtgg
     2401 ggaggccctc acccccgcat ctgcttgcag cctcccccga gcagccggac tccattccct
     2461 ccgcctcgcc tgcccgccct ggagcaggga cctggcggcc tgtgggtgtg gggggccccg
     2521 gctgtagctc agctgttgtg gcctgcaggc ctggggggac ccgggggcag ccgggcagct
     2581 gtcctggtcc agcagtgggt cagttatgct gacacggagc tcatacccgc tgcctgcggg
     2641 gcgaccctgc ctgccctggg acttcgaggt cctggtcagg atccccaggc tgcccttggg
     2701 gccctgggca aggccttgaa ccccttggag gactggctgc ggctgcacac ctacctggct
     2761 ggagatgccc ccactctggc tgacttagct gctgtgacag ccttactgct gcctttccga
     2821 tacgttctgg acccttctgc ccgccggatc tggggcaatg tgactcgctg gtttaacact
     2881 tgtgtccggc aaccggaatt ccgggctgtg ctgggagaag tggccctgta ctcgggggcc
     2941 aggtctgtca ctcaacagcc aggctctgag gtcatcgcac cccaaaaaac acccgcgcag
     3001 ctcaaaaaag aggcaaagaa acgggagaaa ctagagaaat tccagcagaa gcagaagacc
     3061 cagcagcagc ccccgcacgg agagaagaaa ccaaaaccag agaagaagga gaaacgggac
     3121 cctggggtca ttacctatga cctccctacc ccaccggggg agaagaaaga tgtaagtggc
     3181 gccatgcccg actcctacag ccctcagtat gtggaggctg cctggtatcc atggtgggag
     3241 cggcagggct tcttcaagcc agagtacggg cgtcctagtg tgtcagcacc aaatccccgg
     3301 ggtgtcttca tgatgtgcat cccacccccc aacgtgacag gctccctgca cctgggccac
     3361 gcactcacca acgccatcca ggactccctg actcgatggc accgcatgcg tggggagacc
     3421 accctatgga acccaggctg tgaccatgca ggcattgcca cccaggtggt ggtggaaaag
     3481 aagctctgga aagagcgggg tctgaaccgg caccagctgg gccgcgaggc ctttcttgag
     3541 gaggtctgga agtggaaagc cgagaagggt gacaggattt accaccaatt aaagaaactt
     3601 ggcagctcct tggactggga tcgagcctgc ttcaccatgg atcctaaatt gtcagcaact
     3661 gtgacagagg cctttgttag gctccacgaa gaaggggtca tctaccgtag cacccgcctg
     3721 gtcaactggt cctgcaccct caactcagcc atttctgaca ttgaggtgga taagaaggag
     3781 ctgacaggcc gcaccctgct tcctgtgcct ggctacaagg agaaggtgga gtttggggtc
     3841 cttgtgtcct ttgcctacaa ggtccaaggc tcagacagcg acgaggaggt ggtggtggca
     3901 acaacccgga ttgagaccat gctgggagac gtggctgtag ctgtgcaccc caaggatccc
     3961 agataccagc acttaaaggg aaagtgtgtc gtccacccat tcttgtcccg gagccttccc
     4021 atcgtcttcg atgactttgt agacatggag tttggcacag gtgccgtgaa gatcaccccg
     4081 gcacatgacc aaaatgacta tgaggttggg cagcgacaca ggctcgaggc cattagcatc
     4141 atggactcaa agggggccct catcaatgtg cctccacctt tcctgggcct gcccaggttt
     4201 gaggccagga aggctgtgct ggcggcactg aaggagcggg gcctgttccg tggagtcaag
     4261 gacaacccca tggtggtgcc gctttgcaac cgctccaagg atgtggtgga gcctctgtta
     4321 aggccacaat ggtacgtgcg ctgtggggag atggctcagg ctgccagtgc tgctgtgacc
     4381 cggggtgacc tccgtatact gcctgaggcc catcaacgga cgtggcattc ttggatggac
     4441 aacatcagag actggtgcat ctctcggcag ctgtggtggg gccaccgaat ccctgcctac
     4501 tttatcaccg tccatgaccc ggcagtaccc cctggggagg accctgatgg gcggtactgg
     4561 gtaagcggac gcactgaagc agaggcccga gagaaggcag cacgggagtt tggagtgtcc
     4621 cctgacaaga tcagccttca gcaagatgag gatgtgttgg acacctggtt ctcctccggt
     4681 ctctttccct tctccatctt tggctggccc aatcagtcag aagacctcag tgtgttctac
     4741 cctgggaccc tgttggagac aggccatgat atcctcttct tctgggtggc ccggatggtc
     4801 atgctcggcc tgaagctcac tgggaagctg cccttcagag aggtctatct ccatgcaatc
     4861 gtgcgtgatg ctcatggcag gaagatgagc aagtctcttg gcaatgtcat cgaccccctg
     4921 gatgtcatcc atggagtttc cttgcagggc ctctatgacc aactactgaa cagcaacctg
     4981 gatcccagtg aggtggagaa agccaaagaa ggacagaagg ccgactttcc agcagggatt
     5041 cccgagtgtg gcactgatgc cctacgcttt ggactctgtg cctacacatc ccaaggtcga
     5101 gacatcaacc tggatgtgaa caggatcctg gggtaccgtc acttctgcaa caaactctgg
     5161 aatgctacca agtttgccct gcgcggcctt gggaagggct ttgtgccctc agcaacctcc
     5221 aagcctgaag ggcacgagag cctggtggac cgctggatcc gcagccgcct aactgaggcc
     5281 gtgaggctca gcaatgaagg cttccaggct tacgatttcc cggccatcac caccgcccag
     5341 tacagctttt ggctctatga gctctgtgat gtctatttgg agtgtctaaa acctgtgctg
     5401 aatggagtgg accaggtggc ggcagagtgt gctcggcaga ccctctacac ctgcctggat
     5461 gttggcctgc ggctgctctc gcccttcatg ccctttgtca cagaggagct attccagaga
     5521 ctgccccggc ggacaccaaa agcgcctgct agcctctgtg tcaccccgta cccagagccc
     5581 tcagagtgct cctggaagga ccctgaagct gaagctgctc ttgaactagc tctgagcatc
     5641 actcgagctg tgcgctccct gcgtgctgac tacaacctga cccgcaccag gcccgactgt
     5701 ttcttggaag tagccgatga ggccacaggt gccttggcct cggcagtgtc gggctacgtg
     5761 caggctctgg ccagtgcggg cgtggtggct gtcctcgccc tgggtgctcc tgcaccgcag
     5821 ggctgcgctg tagctgtggc ttctgaccgc tgctccatcc acctgcagct gcaggggcta
     5881 gtggacccag cccgggagtt gggcaagctg caggccaagc gaagtgaggc acagcggcag
     5941 gctcagcggc tgcaggagcg ccgtgctgcc tccagctact cagcaaaggt gccccttgag
     6001 gtccaggagg cagatgaagc gaagttgcaa cagacagagg cggagctcag gaaggtggat
     6061 gaggccatcg ccctgttcca gaagatgctg tgaggatccg cggccgcgaa ggatctgcga
     6121 tcgctccggt gcccgtcagt gggcagagcg cacatcgccc acagtccccg agaagttggg
     6181 gggaggggtc ggcaattgaa cgggtgccta gagaaggtgg cgcggggtaa actgggaaag
     6241 tgatgtcgtg tactggctcc gcctttttcc cgagggtggg ggagaaccgt atataagtgc
     6301 agtagtcgcc gtgaacgttc tttttcgcaa cgggtttgcc gccagaacac agctgaagct
     6361 tcgaggggct cgcatctctc cttcacgcgc ccgccgccct acctgaggcc gccatccacg
     6421 ccggttgagt cgcgttctgc cgcctcccgc ctgtggtgcc tcctgaactg cgtccgccgt
     6481 ctaggtaagt ttaaagctca ggtcgagacc gggcctttgt ccggcgctcc cttggagcct
     6541 acctagactc agccggctct ccacgctttg cctgaccctg cttgctcaac tctacgtctt
     6601 tgtttcgttt tctgttctgc gccgttacag atccaagctg tgaccggcgc ctacgctagg
     6661 ccaccatggc tgaacaagat ggattgcacg caggttctcc ggccgcttgg gtggagaggc
     6721 tattcggcta tgactgggca caacagacaa tcggctgctc tgatgccgcc gtgttccggc
     6781 tgtcagcgca ggggcgcccg gttctttttg tcaagaccga cctgtccggt gccctgaatg
     6841 aactgcagga cgaggcagcg cggctatcgt ggctggccac gacgggcgtt ccttgcgcag
     6901 ctgtgctcga cgttgtcact gaagcgggaa gggactggct gctattgggc gaagtgccgg
     6961 ggcaggatct cctgtcatct caccttgctc ctgccgagaa agtatccatc atggctgatg
     7021 caatgcggcg gctgcatacg cttgatccgg ctacctgccc attcgaccac caagcgaaac
     7081 atcgcatcga gcgagcacgt actcggatgg aagccggtct tgtcgatcag gatgatctgg
     7141 acgaagagca tcaggggctc gcgccagccg aactgttcgc caggctcaag gcgcgcatgc
     7201 ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat atcatggtgg
     7261 aaaatggccg cttttctgga ttcatcgact gtggccggct gggtgtggcg gaccgctatc
     7321 aggacatagc gttggctacc cgtgatattg ctgaagagct tggcggcgaa tgggctgacc
     7381 gcttcctcgt gctttacggt atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc
     7441 ttcttgacga gttcttctga gtcgacaatc aacctctgga ttacaaaatt tgtgaaagat
     7501 tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc
     7561 ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct
     7621 ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca
     7681 ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt
     7741 ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg
     7801 cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga
     7861 aatcatcgtc ctttccttgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt
     7921 ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc
     7981 cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt
     8041 gggccgcctc cccgcctggt acctttaaga ccaatgactt acaaggcagc tgtagatctt
     8101 agccactttt taaaagaaaa ggggggactg gaagggctaa ttcactccca acgaaaataa
     8161 gatctgcttt ttgcttgtac tgggtctctc tggttagacc agatctgagc ctgggagctc
     8221 tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa
     8281 gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag
     8341 tcagtgtgga aaatctctag cagtagtagt tcatgtcatc ttattattca gtatttataa
     8401 cttgcaaaga aatgaatatc agagagtgag aggaacttgt ttattgcagc ttataatggt
     8461 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct
     8521 agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggctcta gctatcccgc
     8581 ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt
     8641 atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt
     8701 ttggaggcct agacttttgc agagacggcc caaattcgta atcatggtca tagctgtttc
     8761 ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt
     8821 gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc
     8881 ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg
     8941 ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct
     9001 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
     9061 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
     9121 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
     9181 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
     9241 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
     9301 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
     9361 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
     9421 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
     9481 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
     9541 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg
     9601 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
     9661 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
     9721 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
     9781 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
     9841 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
     9901 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
     9961 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
    10021 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
    10081 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
    10141 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
    10201 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg
    10261 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
    10321 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
    10381 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
    10441 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
    10501 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa
    10561 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
    10621 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
    10681 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
    10741 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
    10801 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
    10861 taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtctaagaa accattatta
    10921 tcatgacatt aacctataaa aataggcgta tcacgaggcc ctttcgtctc gcgcgtttcg
    10981 gtgatgacgg tgaaaacctc tgacacatgc agctcccgga gacggtcaca gcttgtctgt
    11041 aagcggatgc cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc
    11101 ggggctggct taactatgcg gcatcagagc agattgtact gagagtgcac catatgcggt
    11161 gtgaaatacc gcacagatgc gtaaggagaa aataccgcat caggcgccat tcgccattca
    11221 ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta cgccagctgg
    11281 cgaaaggggg atgtgctgca aggcgattaa gttgggtaac gccagggttt tcccagtcac
    11341 gacgttgtaa aacgacggcc agtgccaagc tg
//
