LOCUS       V034488                10724 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V034488
VERSION     V034488
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2303..4600
                     /label="DPP4(NM_001935)"
                     /note="DPP4(NM_001935)"
                     /gene="DPP4"
     CDS             4607..4672
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        4716..4927
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             4940..5208
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     CDS             5243..5950
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /transl_table=1
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
                     /label="mCherry"
     CDS             5960..6013
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             6020..6814
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MAEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     misc_feature    6821..7409
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7481..7714
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    7786..7907
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      7926..8061
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(8099..8115)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8123..8139
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8147..8177)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8192..8213
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8501..9089)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9260..10120)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10121..10225)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     10699..10715
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc gaattcgcca ccatgaagac accgtggaag gttcttctgg gactgctggg
     2341 tgctgctgcg cttgtcacca tcatcaccgt gcccgtggtt ctgctgaaca aaggcacaga
     2401 tgatgctaca gctgacagtc gcaaaactta cactctaact gattacttaa aaaatactta
     2461 tagactgaag ttatactcct taagatggat ttcagatcat gaatatctct acaaacaaga
     2521 aaataatatc ttggtattca atgctgaata tggaaacagc tcagttttct tggagaacag
     2581 tacatttgat gagtttggac attctatcaa tgattattca atatctcctg atgggcagtt
     2641 tattctctta gaatacaact acgtgaagca atggaggcat tcctacacag cttcatatga
     2701 catttatgat ttaaataaaa ggcagctgat tacagaagag aggattccaa acaacacaca
     2761 gtgggtcaca tggtcaccag tgggtcataa attggcatat gtttggaaca atgacattta
     2821 tgttaaaatt gaaccaaatt taccaagtta cagaatcaca tggacgggga aagaagatat
     2881 aatatataat ggaataactg actgggttta tgaagaggaa gtcttcagtg cctactctgc
     2941 tctgtggtgg tctccaaacg gcactttttt agcatatgcc caatttaacg acacagaagt
     3001 cccacttatt gaatactcct tctactctga tgagtcactg cagtacccaa agactgtacg
     3061 ggttccatat ccaaaggcag gagctgtgaa tccaactgta aagttctttg ttgtaaatac
     3121 agactctctc agctcagtca ccaatgcaac ttccatacaa atcactgctc ctgcttctat
     3181 gttgataggg gatcactact tgtgtgatgt gacatgggca acacaagaaa gaatttcttt
     3241 gcagtggctc aggaggattc agaactattc ggtcatggat atttgtgact atgatgaatc
     3301 cagtggaaga tggaactgct tagtggcacg gcaacacatt gaaatgagta ctactggctg
     3361 ggttggaaga tttaggcctt cagaacctca ttttaccctt gatggtaata gcttctacaa
     3421 gatcatcagc aatgaagaag gttacagaca catttgctat ttccaaatag ataaaaaaga
     3481 ctgcacattt attacaaaag gcacctggga agtcatcggg atagaagctc taaccagtga
     3541 ttatctatac tacattagta atgaatataa aggaatgcca ggaggaagga atctttataa
     3601 aatccaactt agtgactata caaaagtgac atgcctcagt tgtgagctga atccggaaag
     3661 gtgtcagtac tattctgtgt cattcagtaa agaggcgaag tattatcagc tgagatgttc
     3721 cggtcctggt ctgcccctct atactctaca cagcagcgtg aatgataaag ggctgagagt
     3781 cctggaagac aattcagctt tggataaaat gctgcagaat gtccagatgc cctccaaaaa
     3841 actggacttc attattttga atgaaacaaa attttggtat cagatgatct tgcctcctca
     3901 ttttgataaa tccaagaaat atcctctact attagatgtg tatgcaggcc catgtagtca
     3961 aaaagcagac actgtcttca gactgaactg ggccacttac cttgcaagca cagaaaacat
     4021 tatagtagct agctttgatg gcagaggaag tggttaccaa ggagataaga tcatgcatgc
     4081 aatcaacaga agactgggaa catttgaagt tgaagatcaa attgaagcag ccagacaatt
     4141 ttcaaaaatg ggatttgtgg acaacaaacg aattgcaatt tggggctggt catatggagg
     4201 gtacgtaacc tcaatggtcc tgggatcggg aagtggcgtg ttcaagtgtg gaatagccgt
     4261 ggcgcctgta tcccggtggg agtactatga ctcagtgtac acagaacgtt acatgggtct
     4321 cccaactcca gaagacaacc ttgaccatta cagaaattca acagtcatga gcagagctga
     4381 aaattttaaa caagttgagt acctccttat tcatggaaca gcagatgata acgttcactt
     4441 tcagcagtca gctcagatct ccaaagccct ggtcgatgtt ggagtggatt tccaggcaat
     4501 gtggtatact gatgaagacc atggaatagc tagcagcaca gcacaccaac atatatatac
     4561 ccacatgagc cacttcataa aacaatgttt ctctttacct ggatccgact acaaagacca
     4621 tgacggtgat tataaagatc atgacatcga ctacaaggat gacgatgaca agtagcggcc
     4681 gcgaaggatc tgcgatcgct ccggtgcccg tcagtgggca gagcgcacat cgcccacagt
     4741 ccccgagaag ttggggggag gggtcggcaa ttgaacgggt gcctagagaa ggtggcgcgg
     4801 ggtaaactgg gaaagtgatg tcgtgtactg gctccgcctt tttcccgagg gtgggggaga
     4861 accgtatata agtgcagtag tcgccgtgaa cgttcttttt cgcaacgggt ttgccgccag
     4921 aacacagctg aagcttcgag gggctcgcat ctctccttca cgcgcccgcc gccctacctg
     4981 aggccgccat ccacgccggt tgagtcgcgt tctgccgcct cccgcctgtg gtgcctcctg
     5041 aactgcgtcc gccgtctagg taagtttaaa gctcaggtcg agaccgggcc tttgtccggc
     5101 gctcccttgg agcctaccta gactcagccg gctctccacg ctttgcctga ccctgcttgc
     5161 tcaactctac gtctttgttt cgttttctgt tctgcgccgt tacagatcca agctgtgacc
     5221 ggcgcctacg ctagacgcca ccatggtgag caagggcgag gaggataaca tggccatcat
     5281 caaggagttc atgcgcttca aggtgcacat ggagggctcc gtgaacggcc acgagttcga
     5341 gatcgagggc gagggcgagg gccgccccta cgagggcacc cagaccgcca agctgaaggt
     5401 gaccaagggt ggccccctgc ccttcgcctg ggacatcctg tcccctcagt tcatgtacgg
     5461 ctccaaggcc tacgtgaagc accccgccga catccccgac tacttgaagc tgtccttccc
     5521 cgagggcttc aagtgggagc gcgtgatgaa cttcgaggac ggcggcgtgg tgaccgtgac
     5581 ccaggactcc tccctgcagg acggcgagtt catctacaag gtgaagctgc gcggcaccaa
     5641 cttcccctcc gacggccccg taatgcagaa gaagaccatg ggctgggagg cctcctccga
     5701 gcggatgtac cccgaggacg gcgccctgaa gggcgagatc aagcagaggc tgaagctgaa
     5761 ggacggcggc cactacgacg ctgaggtcaa gaccacctac aaggccaaga agcccgtgca
     5821 gctgcccggc gcctacaacg tcaacatcaa gttggacatc acctcccaca acgaggacta
     5881 caccatcgtg gaacagtacg aacgcgccga gggccgccac tccaccggcg gcatggacga
     5941 gctgtacaag ggatctcgcg agggcagagg aagtcttcta acatgcggtg acgtggagga
     6001 gaatcccggc cctgccacca tggctgaaca agatggattg cacgcaggtt ctccggccgc
     6061 ttgggtggag aggctattcg gctatgactg ggcacaacag acaatcggct gctctgatgc
     6121 cgccgtgttc cggctgtcag cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc
     6181 cggtgccctg aatgaactgc aggacgaggc agcgcggcta tcgtggctgg ccacgacggg
     6241 cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg ggaagggact ggctgctatt
     6301 gggcgaagtg ccggggcagg atctcctgtc atctcacctt gctcctgccg agaaagtatc
     6361 catcatggct gatgcaatgc ggcggctgca tacgcttgat ccggctacct gcccattcga
     6421 ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg atggaagccg gtcttgtcga
     6481 tcaggatgat ctggacgaag agcatcaggg gctcgcgcca gccgaactgt tcgccaggct
     6541 caaggcgcgc atgcccgacg gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc
     6601 gaatatcatg gtggaaaatg gccgcttttc tggattcatc gactgtggcc ggctgggtgt
     6661 ggcggaccgc tatcaggaca tagcgttggc tacccgtgat attgctgaag agcttggcgg
     6721 cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat
     6781 cgccttctat cgccttcttg acgagttctt ctgagtcgac aatcaacctc tggattacaa
     6841 aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata
     6901 cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc
     6961 cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg
     7021 tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac
     7081 ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat
     7141 cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt
     7201 ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg ccacctggat
     7261 tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc
     7321 ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag
     7381 tcggatctcc ctttgggccg cctccccgcg gtacctttaa gaccaatgac ttacaaggca
     7441 gctgtagatc ttagccactt tttaaaagaa aaggggggac tggaagggct aattcactcc
     7501 caacgaagat aagatctgct ttttgcttgt actgggtctc tctggttaga ccagatctga
     7561 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
     7621 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
     7681 agaccctttt agtcagtgtg gaaaatctct agcagtagta gttcatgtca tcttattatt
     7741 cagtatttat aacttgcaaa gaaatgaata tcagagagtg agaggaactt gtttattgca
     7801 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt
     7861 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggctc
     7921 tagctatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa
     7981 ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt
     8041 gaggaggctt ttttggaggc ctagactttt gcagagacgg cccaaattcg taatcatggt
     8101 catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg
     8161 gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt
     8221 tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg
     8281 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg
     8341 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
     8401 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
     8461 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
     8521 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
     8581 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
     8641 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
     8701 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
     8761 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
     8821 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
     8881 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
     8941 ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
     9001 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
     9061 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
     9121 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
     9181 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
     9241 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
     9301 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
     9361 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc
     9421 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
     9481 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
     9541 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt
     9601 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
     9661 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
     9721 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
     9781 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
     9841 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata
     9901 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
     9961 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
    10021 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
    10081 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
    10141 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
    10201 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag
    10261 aaaccattat tatcatgaca ttaacctata aaaataggcg tatcacgagg ccctttcgtc
    10321 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
    10381 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
    10441 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
    10501 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
    10561 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
    10621 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
    10681 tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa gctg
//
