LOCUS       V039760                11920 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V039760
VERSION     V039760
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2317..5946
                     /EC_number=""
                     /codon_start=1
                     /gene=""
                     /note=""
                     /product="precursor of human epidermal growth factor
                     receptor"
                     /protein_id=""
                     /transl_table=1
                     /translation="MRPSGTAGAALLALLAALCPASRA,LEEKKVCQGTSNKLTQLGTF
                     EDHFLSLQRMFNNCEVVLGNLEITYVQRNYDLSFLKTIQEVAGYVLIALNTVERIPLEN
                     LQIIRGNMYYENSYALAVLSNYDANKTGLKELPMRNLQEILHGAVRFSNNPALCNVESI
                     QWRDIVSSDFLSNMSMDFQNHLGSCQKCDPSCPNGSCWGAGEENCQKLTKIICAQQCSG
                     RCRGKSPSDCCHNQCAAGCTGPRESDCLVCRKFRDEATCKDTCPPLMLYNPTTYQMDVN
                     PEGKYSFGATCVKKCPRNYVVTDHGSCVRACGADSYEMEEDGVRKCKKCEGPCRKVCNG
                     IGIGEFKDSLSINATNIKHFKNCTSISGDLHILPVAFRGDSFTHTPPLDPQELDILKTV
                     KEITGFLLIQAWPENRTDLHAFENLEIIRGRTKQHGQFSLAVVSLNITSLGLRSLKEIS
                     DGDVIISGNKNLCYANTINWKKLFGTSGQKTKIISNRGENSCKATGQVCHALCSPEGCW
                     GPEPRDCVSCRNVSRGRECVDKCNLLEGEPREFVENSECIQCHPECLPQAMNITCTGRG
                     PDNCIQCAHYIDGPHCVKTCPAGVMGENNTLVWKYADAGHVCHLCHPNCTYGCTGPGLE
                     GCPTNGPKIPS,IATGMVGALLLLLVVALGIGLFM,RRRHIVRKRTLRRLLQERELVEP
                     LTPSGEAPNQALLRILKETEFKKIKVLGSGAFGTVYKGLWIPEGEKVKIPVAIKELREA
                     TSPKANKEILDEAYVMASVDNPHVCRLLGICLTSTVQLITQLMPFGCLLDYVREHKDNI
                     GSQYLLNWCVQIAKGMNYLEDRRLVHRDLAARNVLVKTPQHVKITDFGLAKLLGAEEKE
                     YHAEGGKVPIKWMALESILHRIYTHQSDVWSYGVTVWELMTFGSKPYDGIPASEISSIL
                     EKGERLPQPPICTIDVYMIMVKCWMIDADSRPKFRELIIEFSKMARDPQRYLVIQGDER
                     MHLPSPTDSNFYRALMDEEDMDDVVDADEYLIPQQGFFSSPSTSRTPLLSSLSATSNNS
                     TVACIDRNGLQSCPIKEDSFLQRYSSDPTGALTEDSIDDTFLPVPEYINQSVPKRPAGS
                     VQNPVYHNQPLNPAPSRDPHYQDPHSTAVGNPEYLNTVQPTCVNSTFDSPAHWAQKGSH
                     QISLDNPDYQQDFFPKEAKPNGIFKGSTAENAEYLRVAPQSSEFIGA"
                     /label="EGFR"
     CDS             5953..6018
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        6062..6273
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             6286..6554
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      6583..6592
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             6613..7275
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             7345..7398
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             7399..7998
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    7999..8587
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             8661..8894
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    8966..9087
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      9127..9262
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(9295..9311)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    9319..9335
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(9343..9373)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    9388..9409
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(9697..10285)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(10456..11316)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(11317..11421)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     11895..11911
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc ctgatacgaa ctcggaattc gccaccatgc gaccctccgg gacggccggg
     2341 gcagcgctcc tggcgctgct ggctgcgctc tgcccggcga gtcgggctct ggaggaaaag
     2401 aaagtttgcc aaggcacgag taacaagctc acgcagttgg gcacttttga agatcatttt
     2461 ctcagcctcc agaggatgtt caataactgt gaggtggtcc ttgggaattt ggaaattacc
     2521 tatgtgcaga ggaattatga tctttccttc ttaaagacca tccaggaggt ggctggttat
     2581 gtcctcattg ccctcaacac agtggagcga attcctttgg aaaacctgca gatcatcaga
     2641 ggaaatatgt actacgaaaa ttcctatgcc ttagcagtct tatctaacta tgatgcaaat
     2701 aaaaccggac tgaaggagct gcccatgaga aatttacagg aaatcctgca tggcgccgtg
     2761 cggttcagca acaaccctgc cctgtgcaac gtggagagca tccagtggcg ggacatagtc
     2821 agcagtgact ttctcagcaa catgtcgatg gacttccaga accacctggg cagctgccaa
     2881 aagtgtgatc caagctgtcc caatgggagc tgctggggtg caggagagga gaactgccag
     2941 aaactgacca aaatcatctg tgcccagcag tgctccgggc gctgccgtgg caagtccccc
     3001 agtgactgct gccacaacca gtgtgctgca ggctgcacag gcccccggga gagcgactgc
     3061 ctggtctgcc gcaaattccg agacgaagcc acgtgcaagg acacctgccc cccactcatg
     3121 ctctacaacc ccaccacgta ccagatggat gtgaaccccg agggcaaata cagctttggt
     3181 gccacctgcg tgaagaagtg tccccgtaat tatgtggtga cagatcacgg ctcgtgcgtc
     3241 cgagcctgtg gggccgacag ctatgagatg gaggaagacg gcgtccgcaa gtgtaagaag
     3301 tgcgaagggc cttgccgcaa agtgtgtaac ggaataggta ttggtgaatt taaagactca
     3361 ctctccataa atgctacgaa tattaaacac ttcaaaaact gcacctccat cagtggcgat
     3421 ctccacatcc tgccggtggc atttaggggt gactccttca cacatactcc tcctctggat
     3481 ccacaggaac tggatattct gaaaaccgta aaggaaatca cagggttttt gctgattcag
     3541 gcttggcctg aaaacaggac ggacctccat gcctttgaga acctagaaat catacgcggc
     3601 aggaccaagc aacatggtca gttttctctt gcagtcgtca gcctgaacat aacatccttg
     3661 ggattacgct ccctcaagga gataagtgat ggagatgtga taatttcagg aaacaaaaat
     3721 ttgtgctatg caaatacaat aaactggaaa aaactgtttg ggacctccgg tcagaaaacc
     3781 aaaattataa gcaacagagg tgaaaacagc tgcaaggcca caggccaggt ctgccatgcc
     3841 ttgtgctccc ccgagggctg ctggggcccg gagcccaggg actgcgtctc ttgccggaat
     3901 gtcagccgag gcagggaatg cgtggacaag tgcaaccttc tggagggtga gccaagggag
     3961 tttgtggaga actctgagtg catacagtgc cacccagagt gcctgcctca ggccatgaac
     4021 atcacctgca caggacgggg accagacaac tgtatccagt gtgcccacta cattgacggc
     4081 ccccactgcg tcaagacctg cccggcagga gtcatgggag aaaacaacac cctggtctgg
     4141 aagtacgcag acgccggcca tgtgtgccac ctgtgccatc caaactgcac ctacggatgc
     4201 actgggccag gtcttgaagg ctgtccaacg aatgggccta agatcccgtc catcgccact
     4261 gggatggtgg gggccctcct cttgctgctg gtggtggccc tggggatcgg cctcttcatg
     4321 cgaaggcgcc acatcgttcg gaagcgcacg ctgcggaggc tgctgcagga gagggagctt
     4381 gtggagcctc ttacacccag tggagaagct cccaaccaag ctctcttgag gatcttgaag
     4441 gaaactgaat tcaaaaagat caaagtgctg ggctccggtg cgttcggcac ggtgtataag
     4501 ggactctgga tcccagaagg tgagaaagtt aaaattcccg tcgctatcaa ggaattaaga
     4561 gaagcaacat ctccgaaagc caacaaggaa atcctcgatg aagcctacgt gatggccagc
     4621 gtggacaacc cccacgtgtg ccgcctgctg ggcatctgcc tcacctccac cgtgcagctc
     4681 atcacgcagc tcatgccctt cggctgcctc ctggactatg tccgggaaca caaagacaat
     4741 attggctccc agtacctgct caactggtgt gtgcagatcg caaagggcat gaactacttg
     4801 gaggaccgtc gcttggtgca ccgcgacctg gcagccagga acgtactggt gaaaacaccg
     4861 cagcatgtca agatcacaga ttttgggctg gccaaactgc tgggtgcgga agagaaagaa
     4921 taccatgcag aaggaggcaa agtgcctatc aagtggatgg cattggaatc aattttacac
     4981 agaatctata cccaccagag tgatgtctgg agctacgggg tgactgtttg ggagttgatg
     5041 acctttggat ccaagccata tgacggaatc cctgccagcg agatctcctc catcctggag
     5101 aaaggagaac gcctccctca gccacccata tgtaccatcg atgtctacat gatcatggtc
     5161 aagtgctgga tgatagacgc agatagtcgc ccaaagttcc gtgagttgat catcgaattc
     5221 tccaaaatgg cccgagaccc ccagcgctac cttgtcattc agggggatga aagaatgcat
     5281 ttgccaagtc ctacagactc caacttctac cgtgccctga tggatgaaga agacatggac
     5341 gacgtggtgg atgccgacga gtacctcatc ccacagcagg gcttcttcag cagcccctcc
     5401 acgtcacgga ctcccctcct gagctctctg agtgcaacca gcaacaattc caccgtggct
     5461 tgcattgata gaaatgggct gcaaagctgt cccatcaagg aagacagctt cttgcagcga
     5521 tacagctcag accccacagg cgccttgact gaggacagca tagacgacac cttcctccca
     5581 gtgcctgaat acataaacca gtccgttccc aaaaggcccg ctggctctgt gcagaatcct
     5641 gtctatcaca atcagcctct gaaccccgcg cccagcagag acccacacta ccaggacccc
     5701 cacagcactg cagtgggcaa ccccgagtat ctcaacactg tccagcccac ctgtgtcaac
     5761 agcacattcg acagccctgc ccactgggcc cagaaaggca gccaccaaat tagcctggac
     5821 aaccctgact accagcagga cttctttccc aaggaagcca agccaaatgg catctttaag
     5881 ggctccacag ctgaaaatgc agaataccta agggtcgcgc cacaaagcag tgaatttatt
     5941 ggagcaggat ccgactacaa agaccatgac ggtgattata aagatcatga catcgactac
     6001 aaggatgacg atgacaagta gcggccgcga aggatctgcg atcgctccgg tgcccgtcag
     6061 tgggcagagc gcacatcgcc cacagtcccc gagaagttgg ggggaggggt cggcaattga
     6121 acgggtgcct agagaaggtg gcgcggggta aactgggaaa gtgatgtcgt gtactggctc
     6181 cgcctttttc ccgagggtgg gggagaaccg tatataagtg cagtagtcgc cgtgaacgtt
     6241 ctttttcgca acgggtttgc cgccagaaca cagctgaagc ttcgaggggc tcgcatctct
     6301 ccttcacgcg cccgccgccc tacctgaggc cgccatccac gccggttgag tcgcgttctg
     6361 ccgcctcccg cctgtggtgc ctcctgaact gcgtccgccg tctaggtaag tttaaagctc
     6421 aggtcgagac cgggcctttg tccggcgctc ccttggagcc tacctagact cagccggctc
     6481 tccacgcttt gcctgaccct gcttgctcaa ctctacgtct ttgtttcgtt ttctgttctg
     6541 cgccgttaca gatccaagct gtgaccggcg cctacgctag acgccaccat ggagagcgac
     6601 gagagcggcc tgcccgccat ggagatcgag tgccgcatca ccggcaccct gaacggcgtg
     6661 gagttcgagc tggtgggcgg cggagagggc acccccaagc agggccgcat gaccaacaag
     6721 atgaagagca ccaaaggcgc cctgaccttc agcccctacc tgctgagcca cgtgatgggc
     6781 tacggcttct accacttcgg cacctacccc agcggctacg agaacccctt cctgcacgcc
     6841 atcaacaacg gcggctacac caacacccgc atcgagaagt acgaggacgg cggcgtgctg
     6901 cacgtgagct tcagctaccg ctacgaggcc ggccgcgtga tcggcgactt caaggtggtg
     6961 ggcaccggct tccccgagga cagcgtgatc ttcaccgaca agatcatccg cagcaacgcc
     7021 accgtggagc acctgcaccc catgggcgat aacgtgctgg tgggcagctt cgcccgcacc
     7081 ttcagcctgc gcgacggcgg ctactacagc ttcgtggtgg acagccacat gcacttcaag
     7141 agcgccatcc accccagcat cctgcagaac gggggcccca tgttcgcctt ccgccgcgtg
     7201 gaggagctgc acagcaacac cgagctgggc atcgtggagt accagcacgc cttcaagacc
     7261 cccatcgcct tcgccagatc ccgcgctcag tcgtccaatt ctgccgtgga cggcaccgcc
     7321 ggacccggct ccaccggatc tcgcgagggc agaggaagtc ttctaacatg cggtgacgtg
     7381 gaggagaatc ccggccctat gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac
     7441 gacgtcccca gggccgtacg caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc
     7501 cacaccgtcg atccggaccg ccacatcgag cgggtcaccg agctgcaaga actcttcctc
     7561 acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg
     7621 gtctggacca cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc
     7681 atggccgagt tgagcggttc ccggctggcc gcgcagcaac agatggaagg cctcctggcg
     7741 ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac
     7801 cagggcaagg gtctgggcag cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc
     7861 ggggtgcccg ccttcctgga gacctccgcg ccccgcaacc tccccttcta cgagcggctc
     7921 ggcttcaccg tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc
     7981 cgcaagcccg gtgcctgaaa tcaacctctg gattacaaaa tttgtgaaag attgactggt
     8041 attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat
     8101 catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg
     8161 tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt
     8221 gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact
     8281 ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc
     8341 tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaaatcatcg
     8401 tcctttcctt ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc
     8461 tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg
     8521 cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc
     8581 tccccgcctg gtacctttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt
     8641 tttaaaagaa aaggggggac tggaagggct aattcactcc caacgaagat aagatctgct
     8701 ttttgcttgt actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta
     8761 actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg
     8821 tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg
     8881 gaaaatctct agcagtagta gttcatgtca tcttattatt cagtatttat aacttgcaaa
     8941 gaaatgaata tcagagagtg agaggaactt gtttattgca gcttataatg gttacaaata
     9001 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
     9061 tttgtccaaa ctcatcaatg tatcttatca tgtctggctc tagctatccc gcccctaact
     9121 ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta
     9181 atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag
     9241 tgaggaggct tttttggagg cctagacttt tgcagagacc aaattcgtaa tcatgtcata
     9301 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag
     9361 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg
     9421 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca
     9481 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc
     9541 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
     9601 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
     9661 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
     9721 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
     9781 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
     9841 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
     9901 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
     9961 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
    10021 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
    10081 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac
    10141 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
    10201 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
    10261 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
    10321 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
    10381 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
    10441 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
    10501 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
    10561 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
    10621 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
    10681 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
    10741 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt
    10801 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
    10861 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
    10921 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
    10981 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
    11041 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
    11101 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
    11161 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
    11221 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
    11281 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
    11341 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
    11401 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac
    11461 cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtctcgc
    11521 gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga cggtcacagc
    11581 ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg
    11641 cgggtgtcgg ggctggctta actatgcggc atcagagcag attgtactga gagtgcacca
    11701 tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcgccattc
    11761 gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg
    11821 ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc
    11881 ccagtcacga cgttgtaaaa cgacggccag tgccaagctg
//
