LOCUS V046685 11076 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V046685 VERSION V046685 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers LTR 234..414 /note="truncated 5' long terminal repeat (LTR) from HIV-1" /label="5' LTR (truncated)" misc_feature 458..583 /note="packaging signal of human immunodeficiency virus type 1" /label="HIV-1 Ψ" misc_feature 1076..1309 /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." /label="RRE" CDS 1493..1537 /codon_start=1 /note="recognized by the 2H10 single-chain llama nanobody" /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /transl_table=1 /translation="KNEQELLELDKWASL" /label="gp41 peptide" misc_feature 1804..1920 /note="central polypurine tract and central termination sequence of HIV-1" /label="cPPT/CTS" promoter 2010..2213 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" CDS 2301..5930 /EC_number="" /codon_start=1 /gene="" /note="" /product="precursor of human epidermal growth factor receptor" /protein_id="" /transl_table=1 /translation="MRPSGTAGAALLALLAALCPASRA,LEEKKVCQGTSNKLTQLGTF EDHFLSLQRMFNNCEVVLGNLEITYVQRNYDLSFLKTIQEVAGYVLIALNTVERIPLEN LQIIRGNMYYENSYALAVLSNYDANKTGLKELPMRNLQEILHGAVRFSNNPALCNVESI QWRDIVSSDFLSNMSMDFQNHLGSCQKCDPSCPNGSCWGAGEENCQKLTKIICAQQCSG RCRGKSPSDCCHNQCAAGCTGPRESDCLVCRKFRDEATCKDTCPPLMLYNPTTYQMDVN PEGKYSFGATCVKKCPRNYVVTDHGSCVRACGADSYEMEEDGVRKCKKCEGPCRKVCNG IGIGEFKDSLSINATNIKHFKNCTSISGDLHILPVAFRGDSFTHTPPLDPQELDILKTV KEITGFLLIQAWPENRTDLHAFENLEIIRGRTKQHGQFSLAVVSLNITSLGLRSLKEIS DGDVIISGNKNLCYANTINWKKLFGTSGQKTKIISNRGENSCKATGQVCHALCSPEGCW GPEPRDCVSCRNVSRGRECVDKCNLLEGEPREFVENSECIQCHPECLPQAMNITCTGRG PDNCIQCAHYIDGPHCVKTCPAGVMGENNTLVWKYADAGHVCHLCHPNCTYGCTGPGLE GCPTNGPKIPS,IATGMVGALLLLLVVALGIGLFM,RRRHIVRKRTLRRLLQERELVEP LTPSGEAPNQALLRILKETEFKKIKVLGSGAFGTVYKGLWIPEGEKVKIPVAIKELREA TSPKANKEILDEAYVMASVDNPHVCRLLGICLTSTVQLITQLMPFGCLLDYVREHKDNI GSQYLLNWCVQIAKGMNYLEDRRLVHRDLAARNVLVKTPQHVKITDFGLAKLLGAEEKE YHAEGGKVPIKWMALESILHRIYTHQSDVWSYGVTVWELMTFGSKPYDGIPASEISSIL EKGERLPQPPICTIDVYMIMVKCWMIDADSRPKFRELIIEFSKMARDPQRYLVIQGDER MHLPSPTDSNFYRALMDEEDMDDVVDADEYLIPQQGFFSSPSTSRTPLLSSLSATSNNS TVACIDRNGLQSCPIKEDSFLQRYSSDPTGALTEDSIDDTFLPVPEYINQSVPKRPAGS VQNPVYHNQPLNPAPSRDPHYQDPHSTAVGNPEYLNTVQPTCVNSTFDSPAHWAQKGSH QISLDNPDYQQDFFPKEAKPNGIFKGSTAENAEYLRVAPQSSEFIGA" /label="EGFR" CDS 5937..6002 /codon_start=1 /product="three tandem FLAG® epitope tags, followed by an enterokinase cleavage site" /transl_table=1 /translation="DYKDHDGDYKDHDIDYKDDDDK" /label="3xFLAG" promoter 6046..6257 /note="core promoter for human elongation factor EF-1α" /label="EF-1α core promoter" LTR 6270..6538 /note="truncated 5' long terminal repeat (LTR) from human T-cell leukemia virus (HTLV) type 1" /label="5' LTR (truncated)" CDS 6565..7164 /codon_start=1 /gene="pac from Streptomyces alboniger" /note="confers resistance to puromycin" /product="puromycin N-acetyltransferase" /transl_table=1 /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*" /label="PuroR" misc_feature 7171..7759 /note="woodchuck hepatitis virus posttranscriptional regulatory element" /label="WPRE" LTR 7833..8066 /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" /label="3' LTR (ΔU3)" polyA_signal 8138..8259 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" rep_origin 8278..8413 /note="SV40 origin of replication" /label="SV40 ori" primer_bind complement(8451..8467) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 8475..8491 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(8499..8529) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 8544..8565 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(8853..9441) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(9612..10472) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(10473..10577) /gene="bla" /label="AmpR promoter" primer_bind 11051..11067 /note="common sequencing primer, one of multiple similar variants" /label="M13 fwd" ORIGIN 1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt 1921 atcgatacta gtattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca 1981 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc 2041 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga 2101 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat 2161 tgacgcaaat gggcggtagg cgtgtacggt gggaggttta tataagcaga gctcgtttag 2221 tgaccgtcag atcgcctgga gacgccatcc acgctgtttt gacctccata gaagattcta 2281 gagctagcga attcgccacc atgcgaccct ccgggacggc cggggcagcg ctcctggcgc 2341 tgctggctgc gctctgcccg gcgagtcggg ctctggagga aaagaaagtt tgccaaggca 2401 cgagtaacaa gctcacgcag ttgggcactt ttgaagatca ttttctcagc ctccagagga 2461 tgttcaataa ctgtgaggtg gtccttggga atttggaaat tacctatgtg cagaggaatt 2521 atgatctttc cttcttaaag accatccagg aggtggctgg ttatgtcctc attgccctca 2581 acacagtgga gcgaattcct ttggaaaacc tgcagatcat cagaggaaat atgtactacg 2641 aaaattccta tgccttagca gtcttatcta actatgatgc aaataaaacc ggactgaagg 2701 agctgcccat gagaaattta caggaaatcc tgcatggcgc cgtgcggttc agcaacaacc 2761 ctgccctgtg caacgtggag agcatccagt ggcgggacat agtcagcagt gactttctca 2821 gcaacatgtc gatggacttc cagaaccacc tgggcagctg ccaaaagtgt gatccaagct 2881 gtcccaatgg gagctgctgg ggtgcaggag aggagaactg ccagaaactg accaaaatca 2941 tctgtgccca gcagtgctcc gggcgctgcc gtggcaagtc ccccagtgac tgctgccaca 3001 accagtgtgc tgcaggctgc acaggccccc gggagagcga ctgcctggtc tgccgcaaat 3061 tccgagacga agccacgtgc aaggacacct gccccccact catgctctac aaccccacca 3121 cgtaccagat ggatgtgaac cccgagggca aatacagctt tggtgccacc tgcgtgaaga 3181 agtgtccccg taattatgtg gtgacagatc acggctcgtg cgtccgagcc tgtggggccg 3241 acagctatga gatggaggaa gacggcgtcc gcaagtgtaa gaagtgcgaa gggccttgcc 3301 gcaaagtgtg taacggaata ggtattggtg aatttaaaga ctcactctcc ataaatgcta 3361 cgaatattaa acacttcaaa aactgcacct ccatcagtgg cgatctccac atcctgccgg 3421 tggcatttag gggtgactcc ttcacacata ctcctcctct ggatccacag gaactggata 3481 ttctgaaaac cgtaaaggaa atcacagggt ttttgctgat tcaggcttgg cctgaaaaca 3541 ggacggacct ccatgccttt gagaacctag aaatcatacg cggcaggacc aagcaacatg 3601 gtcagttttc tcttgcagtc gtcagcctga acataacatc cttgggatta cgctccctca 3661 aggagataag tgatggagat gtgataattt caggaaacaa aaatttgtgc tatgcaaata 3721 caataaactg gaaaaaactg tttgggacct ccggtcagaa aaccaaaatt ataagcaaca 3781 gaggtgaaaa cagctgcaag gccacaggcc aggtctgcca tgccttgtgc tcccccgagg 3841 gctgctgggg cccggagccc agggactgcg tctcttgccg gaatgtcagc cgaggcaggg 3901 aatgcgtgga caagtgcaac cttctggagg gtgagccaag ggagtttgtg gagaactctg 3961 agtgcataca gtgccaccca gagtgcctgc ctcaggccat gaacatcacc tgcacaggac 4021 ggggaccaga caactgtatc cagtgtgccc actacattga cggcccccac tgcgtcaaga 4081 cctgcccggc aggagtcatg ggagaaaaca acaccctggt ctggaagtac gcagacgccg 4141 gccatgtgtg ccacctgtgc catccaaact gcacctacgg atgcactggg ccaggtcttg 4201 aaggctgtcc aacgaatggg cctaagatcc cgtccatcgc cactgggatg gtgggggccc 4261 tcctcttgct gctggtggtg gccctgggga tcggcctctt catgcgaagg cgccacatcg 4321 ttcggaagcg cacgctgcgg aggctgctgc aggagaggga gcttgtggag cctcttacac 4381 ccagtggaga agctcccaac caagctctct tgaggatctt gaaggaaact gaattcaaaa 4441 agatcaaagt gctgggctcc ggtgcgttcg gcacggtgta taagggactc tggatcccag 4501 aaggtgagaa agttaaaatt cccgtcgcta tcaaggaatt aagagaagca acatctccga 4561 aagccaacaa ggaaatcctc gatgaagcct acgtgatggc cagcgtggac aacccccacg 4621 tgtgccgcct gctgggcatc tgcctcacct ccaccgtgca gctcatcacg cagctcatgc 4681 ccttcggctg cctcctggac tatgtccggg aacacaaaga caatattggc tcccagtacc 4741 tgctcaactg gtgtgtgcag atcgcaaagg gcatgaacta cttggaggac cgtcgcttgg 4801 tgcaccgcga cctggcagcc aggaacgtac tggtgaaaac accgcagcat gtcaagatca 4861 cagattttgg gctggccaaa ctgctgggtg cggaagagaa agaataccat gcagaaggag 4921 gcaaagtgcc tatcaagtgg atggcattgg aatcaatttt acacagaatc tatacccacc 4981 agagtgatgt ctggagctac ggggtgactg tttgggagtt gatgaccttt ggatccaagc 5041 catatgacgg aatccctgcc agcgagatct cctccatcct ggagaaagga gaacgcctcc 5101 ctcagccacc catatgtacc atcgatgtct acatgatcat ggtcaagtgc tggatgatag 5161 acgcagatag tcgcccaaag ttccgtgagt tgatcatcga attctccaaa atggcccgag 5221 acccccagcg ctaccttgtc attcaggggg atgaaagaat gcatttgcca agtcctacag 5281 actccaactt ctaccgtgcc ctgatggatg aagaagacat ggacgacgtg gtggatgccg 5341 acgagtacct catcccacag cagggcttct tcagcagccc ctccacgtca cggactcccc 5401 tcctgagctc tctgagtgca accagcaaca attccaccgt ggcttgcatt gatagaaatg 5461 ggctgcaaag ctgtcccatc aaggaagaca gcttcttgca gcgatacagc tcagacccca 5521 caggcgcctt gactgaggac agcatagacg acaccttcct cccagtgcct gaatacataa 5581 accagtccgt tcccaaaagg cccgctggct ctgtgcagaa tcctgtctat cacaatcagc 5641 ctctgaaccc cgcgcccagc agagacccac actaccagga cccccacagc actgcagtgg 5701 gcaaccccga gtatctcaac actgtccagc ccacctgtgt caacagcaca ttcgacagcc 5761 ctgcccactg ggcccagaaa ggcagccacc aaattagcct ggacaaccct gactaccagc 5821 aggacttctt tcccaaggaa gccaagccaa atggcatctt taagggctcc acagctgaaa 5881 atgcagaata cctaagggtc gcgccacaaa gcagtgaatt tattggagca ggatccgact 5941 acaaagacca tgacggtgat tataaagatc atgacatcga ctacaaggat gacgatgaca 6001 agtagcggcc gcgaaggatc tgcgatcgct ccggtgcccg tcagtgggca gagcgcacat 6061 cgcccacagt ccccgagaag ttggggggag gggtcggcaa ttgaacgggt gcctagagaa 6121 ggtggcgcgg ggtaaactgg gaaagtgatg tcgtgtactg gctccgcctt tttcccgagg 6181 gtgggggaga accgtatata agtgcagtag tcgccgtgaa cgttcttttt cgcaacgggt 6241 ttgccgccag aacacagctg aagcttcgag gggctcgcat ctctccttca cgcgcccgcc 6301 gccctacctg aggccgccat ccacgccggt tgagtcgcgt tctgccgcct cccgcctgtg 6361 gtgcctcctg aactgcgtcc gccgtctagg taagtttaaa gctcaggtcg agaccgggcc 6421 tttgtccggc gctcccttgg agcctaccta gactcagccg gctctccacg ctttgcctga 6481 ccctgcttgc tcaactctac gtctttgttt cgttttctgt tctgcgccgt tacagatcca 6541 agctgtgacc ggcgcctacg ctagatgacc gagtacaagc ccacggtgcg cctcgccacc 6601 cgcgacgacg tcccccgggc cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc 6661 acgcgccaca ccgtcgaccc ggaccgccac atcgagcggg tcaccgagct gcaagaactc 6721 ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg tcgcggacga cggcgccgcg 6781 gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc 6841 ccgcgcatgg ccgagttgag cggttcccgg ctggccgcgc agcaacagat ggaaggcctc 6901 ctggcgccgc accggcccaa ggagcccgcg tggttcctgg ccaccgtcgg cgtctcgccc 6961 gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag 7021 cgcgccgggg tgcccgcctt cctggagacc tccgcgcccc gcaacctccc cttctacgag 7081 cggctcggct tcaccgtcac cgccgacgtc gaggtgcccg aaggaccgcg cacctggtgc 7141 atgacccgca agcccggtgc ctgagtcgac aatcaacctc tggattacaa aatttgtgaa 7201 agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta 7261 atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa 7321 tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg 7381 tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc 7441 ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc 7501 cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg 7561 gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg ccacctggat tctgcgcggg 7621 acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg 7681 ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc 7741 ctttgggccg cctccccgcc tggtaccttt aagaccaatg acttacaagg cagctgtaga 7801 tcttagccac tttttaaaag aaaagggggg actggaaggg ctaattcact cccaacgaaa 7861 ataagatctg ctttttgctt gtactgggtc tctctggtta gaccagatct gagcctggga 7921 gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct 7981 tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt 8041 ttagtcagtg tggaaaatct ctagcagtag tagttcatgt catcttatta ttcagtattt 8101 ataacttgca aagaaatgaa tatcagagag tgagaggaac ttgtttattg cagcttataa 8161 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca 8221 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctggc tctagctatc 8281 ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt 8341 atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc 8401 ttttttggag gcctagactt ttgcagagac ggcccaaatt cgtaatcatg gtcatagctg 8461 tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata 8521 aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca 8581 ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc 8641 gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg 8701 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta 8761 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc 8821 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag 8881 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac 8941 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc 9001 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt 9061 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc 9121 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga 9181 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta 9241 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta 9301 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga 9361 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg 9421 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag 9481 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc 9541 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact 9601 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt 9661 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta 9721 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta 9781 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc 9841 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat 9901 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt 9961 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg 10021 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca 10081 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta 10141 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg 10201 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact 10261 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg 10321 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt 10381 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga 10441 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc 10501 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa 10561 caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta agaaaccatt 10621 attatcatga cattaaccta taaaaatagg cgtatcacga ggccctttcg tctcgcgcgt 10681 ttcggtgatg acggtgaaaa cctctgacac atgcagctcc cggagacggt cacagcttgt 10741 ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg 10801 tgtcggggct ggcttaacta tgcggcatca gagcagattg tactgagagt gcaccatatg 10861 cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc gcatcaggcg ccattcgcca 10921 ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct attacgccag 10981 ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg gttttcccag 11041 tcacgacgtt gtaaaacgac ggccagtgcc aagctg //