LOCUS       V046231                10045 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V046231
VERSION     V046231
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2317..4071
                     /label="EIF2A(NM_032025)"
                     /note="EIF2A(NM_032025)"
                     /gene="EIF2A"
     CDS             4078..4143
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        4187..4398
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             4411..4679
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      4708..4717
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             4738..5400
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             5470..5523
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             5524..6123
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    6124..6712
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             6786..7019
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    7091..7212
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      7252..7387
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(7420..7436)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    7444..7460
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(7468..7498)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    7513..7534
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(7822..8410)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(8581..9441)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(9442..9546)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     10020..10036
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc ctgatacgaa ctcggaattc gccaccatgg cgccgtccac gccgctcttg
     2341 acagtccgag gatcagaagg actgtacatg gtgaatggac caccacattt tacagaaagc
     2401 acagtgtttc caagggaatc tgggaagaat tgcaaagtct gtatctttag taaggatggg
     2461 accttgtttg cctggggcaa tggagaaaaa gtaaatatta tcagtgtcac taacaaggga
     2521 ctactgcact ccttcgacct cctgaaggca gtttgccttg aattctcacc caaaaatact
     2581 gtcctggcaa cgtggcagcc ttacactact tctaaagatg gcacagctgg gatacccaac
     2641 ctacaacttt atgatgtgaa aactgggaca tgtttgaaat ctttcatcca gaaaaaaatg
     2701 caaaattggt gtccatcctg gtcagaagat gaaactcttt gtgcccgcaa tgttaacaat
     2761 gaagttcact tctttgaaaa caacaatttt aacacaattg caaataaatt gcatttgcaa
     2821 aaaattaatg attttgtatt atcacctgga ccccaaccat acaaggtggc tgtctatgtt
     2881 ccaggaagta aaggtgcacc ttcatttgtt agattatatc agtaccccaa ctttgctgga
     2941 cctcatgcag ctttagctaa taaaagtttc tttaaggcag ataaagttac aatgctgtgg
     3001 aataaaaaag ctactgctgt gttggtaata gctagcacag atgttgacaa gacaggagct
     3061 tcctactatg gagaacaaac tctacactac attgcaacaa atggagaaag tgctgtagtg
     3121 caattaccaa aaaatggccc catttatgat gtagtttgga attctagttc tactgagttt
     3181 tgtgctgtat atggttttat gcctgccaaa gcgacaattt tcaacttgaa atgtgatcct
     3241 gtatttgact ttggaactgg tcctcgtaat gcagcctact atagccctca tggacatata
     3301 ttagtattag ctggatttgg aaatctgagg ggacaaatgg aagtgtggga tgtgaaaaac
     3361 tacaaactta tttctaaacc ggtggcttct gattctacat attttgcttg gtgcccggat
     3421 ggtgagcata ttttaacagc tacatgtgct cccaggttac gggttaataa tggatacaaa
     3481 atttggcatt atactggctc tatcttgcac aagtatgatg tgccatcaaa tgcagaatta
     3541 tggcaggttt cttggcagcc atttttggat ggaatatttc cagcaaaaac aataacttac
     3601 caagcagttc caagtgaagt acccaatgag gaacctaaag ttgcaacagc ttatagaccc
     3661 ccagctttaa gaaataaacc aatcaccaat tccaaattgc atgaagagga accacctcag
     3721 aatatgaaac cacaatcagg aaacgataag ccattatcaa aaacagctct taaaaatcaa
     3781 aggaagcatg aagctaagaa agctgcaaag caggaagcaa gaagtgacaa gagtccagat
     3841 ttggcaccta ctcctgcccc acagagcaca ccacgaaaca ctgtctctca gtcaatttct
     3901 ggggaccctg agatagacaa aaaaatcaag aacctaaaga agaaactgaa agcaatcgaa
     3961 caactgaaag aacaagcagc aactggaaaa cagctagaaa aaaatcagtt ggagaaaatt
     4021 cagaaagaaa cagcccttct ccaggagctg gaagatttgg aattgggtat tggatccgac
     4081 tacaaagacc atgacggtga ttataaagat catgacatcg actacaagga tgacgatgac
     4141 aagtagcggc cgcgaaggat ctgcgatcgc tccggtgccc gtcagtgggc agagcgcaca
     4201 tcgcccacag tccccgagaa gttgggggga ggggtcggca attgaacggg tgcctagaga
     4261 aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact ggctccgcct ttttcccgag
     4321 ggtgggggag aaccgtatat aagtgcagta gtcgccgtga acgttctttt tcgcaacggg
     4381 tttgccgcca gaacacagct gaagcttcga ggggctcgca tctctccttc acgcgcccgc
     4441 cgccctacct gaggccgcca tccacgccgg ttgagtcgcg ttctgccgcc tcccgcctgt
     4501 ggtgcctcct gaactgcgtc cgccgtctag gtaagtttaa agctcaggtc gagaccgggc
     4561 ctttgtccgg cgctcccttg gagcctacct agactcagcc ggctctccac gctttgcctg
     4621 accctgcttg ctcaactcta cgtctttgtt tcgttttctg ttctgcgccg ttacagatcc
     4681 aagctgtgac cggcgcctac gctagacgcc accatggaga gcgacgagag cggcctgccc
     4741 gccatggaga tcgagtgccg catcaccggc accctgaacg gcgtggagtt cgagctggtg
     4801 ggcggcggag agggcacccc caagcagggc cgcatgacca acaagatgaa gagcaccaaa
     4861 ggcgccctga ccttcagccc ctacctgctg agccacgtga tgggctacgg cttctaccac
     4921 ttcggcacct accccagcgg ctacgagaac cccttcctgc acgccatcaa caacggcggc
     4981 tacaccaaca cccgcatcga gaagtacgag gacggcggcg tgctgcacgt gagcttcagc
     5041 taccgctacg aggccggccg cgtgatcggc gacttcaagg tggtgggcac cggcttcccc
     5101 gaggacagcg tgatcttcac cgacaagatc atccgcagca acgccaccgt ggagcacctg
     5161 caccccatgg gcgataacgt gctggtgggc agcttcgccc gcaccttcag cctgcgcgac
     5221 ggcggctact acagcttcgt ggtggacagc cacatgcact tcaagagcgc catccacccc
     5281 agcatcctgc agaacggggg ccccatgttc gccttccgcc gcgtggagga gctgcacagc
     5341 aacaccgagc tgggcatcgt ggagtaccag cacgccttca agacccccat cgccttcgcc
     5401 agatcccgcg ctcagtcgtc caattctgcc gtggacggca ccgccggacc cggctccacc
     5461 ggatctcgcg agggcagagg aagtcttcta acatgcggtg acgtggagga gaatcccggc
     5521 cctatgaccg agtacaagcc cacggtgcgc ctcgccaccc gcgacgacgt ccccagggcc
     5581 gtacgcaccc tcgccgccgc gttcgccgac taccccgcca cgcgccacac cgtcgatccg
     5641 gaccgccaca tcgagcgggt caccgagctg caagaactct tcctcacgcg cgtcgggctc
     5701 gacatcggca aggtgtgggt cgcggacgac ggcgccgcgg tggcggtctg gaccacgccg
     5761 gagagcgtcg aagcgggggc ggtgttcgcc gagatcggcc cgcgcatggc cgagttgagc
     5821 ggttcccggc tggccgcgca gcaacagatg gaaggcctcc tggcgccgca ccggcccaag
     5881 gagcccgcgt ggttcctggc caccgtcggc gtctcgcccg accaccaggg caagggtctg
     5941 ggcagcgccg tcgtgctccc cggagtggag gcggccgagc gcgccggggt gcccgccttc
     6001 ctggagacct ccgcgccccg caacctcccc ttctacgagc ggctcggctt caccgtcacc
     6061 gccgacgtcg aggtgcccga aggaccgcgc acctggtgca tgacccgcaa gcccggtgcc
     6121 tgaaatcaac ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt
     6181 gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc
     6241 cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag
     6301 ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc
     6361 actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc
     6421 cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg
     6481 ctgttgggca ctgacaattc cgtggtgttg tcggggaaat catcgtcctt tccttggctg
     6541 ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc
     6601 ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt
     6661 cttcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc gcctggtacc
     6721 tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaaggg
     6781 gggactggaa gggctaattc actcccaacg aagataagat ctgctttttg cttgtactgg
     6841 gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact
     6901 gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg
     6961 tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag
     7021 tagtagttca tgtcatctta ttattcagta tttataactt gcaaagaaat gaatatcaga
     7081 gagtgagagg aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac
     7141 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat
     7201 caatgtatct tatcatgtct ggctctagct atcccgcccc taactccgcc catcccgccc
     7261 ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat
     7321 gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt
     7381 ggaggcctag acttttgcag agaccaaatt cgtaatcatg tcatagctgt ttcctgtgtg
     7441 aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc
     7501 ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt
     7561 ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg
     7621 cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt
     7681 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
     7741 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
     7801 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
     7861 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
     7921 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
     7981 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
     8041 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
     8101 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
     8161 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
     8221 agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg
     8281 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
     8341 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
     8401 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
     8461 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
     8521 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag
     8581 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
     8641 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
     8701 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa
     8761 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
     8821 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
     8881 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
     8941 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
     9001 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
     9061 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
     9121 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
     9181 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
     9241 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
     9301 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
     9361 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
     9421 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
     9481 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
     9541 tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa gaaaccatta ttatcatgac
     9601 attaacctat aaaaataggc gtatcacgag gccctttcgt ctcgcgcgtt tcggtgatga
     9661 cggtgaaaac ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga
     9721 tgccgggagc agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggctg
     9781 gcttaactat gcggcatcag agcagattgt actgagagtg caccatatgc ggtgtgaaat
     9841 accgcacaga tgcgtaagga gaaaataccg catcaggcgc cattcgccat tcaggctgcg
     9901 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
     9961 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
    10021 taaaacgacg gccagtgcca agctg
//
