LOCUS       V030030                12416 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V030030
VERSION     V030030
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1920
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2010..2213
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2302..7248
                     /label="EIF2AK4(NM_001013703)"
                     /note="EIF2AK4(NM_001013703)"
                     /gene="EIF2AK4"
     CDS             7255..7281
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             7285..7311
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             7315..7341
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     promoter        7386..7597
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             7610..7878
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     CDS             7905..8504
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    8511..9099
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             9173..9406
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    9478..9599
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      9618..9753
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(9791..9807)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    9815..9831
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(9839..9869)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    9884..9905
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(10193..10781)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(10952..11812)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(11813..11917)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     12391..12407
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt
     1921 atcgatacta gtattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
     1981 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
     2041 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
     2101 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
     2161 tgacgcaaat gggcggtagg cgtgtacggt gggaggttta tataagcaga gctcgtttag
     2221 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagattct
     2281 agagctagcg aattcgccac catggctggg ggccgtgggg cccccgggcg cggccgggac
     2341 gagcctccgg agagctaccc gcaacgacag gaccacgagc tacaggccct ggaggccatt
     2401 tacggcgcgg acttccaaga cctgcggccg gacgcttgcg gaccggtcaa agagccccct
     2461 gaaatcaatt tagttttgta ccctcaaggc ctaactggtg aagaagtata tgtaaaagtg
     2521 gatttgaggg ttaaatgccc acctacctat ccagatgtag ttcctgaaat agagttaaaa
     2581 aatgccaaag gtctatcaaa tgaaagtgtc aatttgttaa aatctcgcct agaagaactg
     2641 gccaagaaac actgtgggga ggtgatgatc tttgaactgg cttaccacgt gcagtcattt
     2701 ctcagcgagc ataacaagcc ccctcccaag tcttttcatg aagaaatgct ggaaaggcgg
     2761 gctcaggagg agcagcagag gctgttggag gccaagcgga aagaagagca ggagcaacgt
     2821 gaaatcctgc atgagattca gagaaggaaa gaagagataa aagaagagaa aaaaaggaaa
     2881 gaaatggcta agcaggaacg tttggaaatt gctagtttgt caaaccaaga tcatacctct
     2941 aagaaggacc caggaggaca cagaacggct gccattctac atggaggctc tcctgacttt
     3001 gtaggaaatg gtaaacatcg ggcaaactcc tcaggaaggt ctaggcgaga acgtcagtat
     3061 tctgtatgta atagtgaaga ttctcctggc tcttgtgaaa ttctgtattt caatatgggg
     3121 agtcctgatc agctcatggt gcacaaaggg aaatgtattg gcagtgatga acaacttgga
     3181 aaattagtct acaatgcttt ggaaacagcc actggtggct ttgtcttgtt gtatgagtgg
     3241 gtccttcagt ggcagaaaaa aatgggtcca ttccttacca gtcaagaaaa agagaagatt
     3301 gataagtgca aaaagcagat tcaaggaaca gaaacagaat tcaactcact ggtaaaattg
     3361 agccatccaa atgtagtacg ctaccttgca atgaatctca aagagcaaga cgactccatc
     3421 gtggtggaca ttttagtgga gcacattagt ggggtctctc ttgctgcaca cctgagccac
     3481 tcaggcccca tccctgtgca tcagcttcgc aggtacacag ctcagctcct gtcaggcctt
     3541 gattatctgc acagcaattc tgtggtgcat aaggtcctga gtgcatctaa tgtcttggtg
     3601 gatgcagaag gcaccgtcaa gattacggac tatagcattt ctaagcgcct cgcagacatt
     3661 tgcaaggagg atgtgtttga gcaaacccga gttcgtttta gtgacaatgc tctgccttat
     3721 aaaacgggga agaaaggaga tgtttggcgt cttggccttc tgctgctgtc cctcagccaa
     3781 ggacaggaat gtggagagta ccctgtgacc atccctagtg acttaccagc tgactttcaa
     3841 gattttctaa agaaatgtgt gtgcttggat gacaaggaaa gatggagtcc ccagcagttg
     3901 ttgaaacaca gctttataaa tccccagcca aaaatgcctc tagtggaaca aagtcctgaa
     3961 gattctgaag gacaagatta tgttgagact gttattccta gcaaccggct acccagtgct
     4021 gccttcttta gtgagacaca gagacagttt tcccgatact tcattgagtt tgaagaatta
     4081 caacttcttg gtaaaggagc ttttggagct gtcatcaagg tgcagaacaa gttggacggc
     4141 tgctgctacg cagtgaagcg catccccatc aacccggcca gccggcagtt ccgcaggatc
     4201 aagggcgaag tgacactgct gtcacggctg caccatgaga acattgtgcg ctactacaac
     4261 gcctggatcg agcggcacga gcggccggcg ggaccgggga cgccgccccc ggactccggg
     4321 cccctggcca aggatgaccg agctgcacgc gggcagccgg cgagcgacac agacggcctg
     4381 gacagcgtag aggccgccgc gccgccaccc atcctcagca gctcggtgga gtggagcact
     4441 tcgggcgagc gctcggccag tgcccgtttc cccgccaccg gcccgggctc cagcgatgac
     4501 gaggacgacg acgaggacga gcacggtggc gtcttctccc agtccttcct gcctgcttca
     4561 gattctgaaa gtgatattat ctttgacaat gaagatgaga acagtaaaag tcagaatcag
     4621 gatgaagatt gcaatgaaaa gaatggctgc catgaaagtg agccatcagt gacgactgag
     4681 gctgtgcact acctatacat ccagatggag tactgtgaga agagcacttt acgagacacc
     4741 attgaccagg gactgtatcg agacaccgtc agactctgga ggctttttcg agagattctg
     4801 gatggattag cttatatcca tgagaaagga atgattcacc gggatttgaa gcctgtcaac
     4861 atttttttgg attctgatga ccatgtgaaa ataggtgatt ttggtttggc gacagaccat
     4921 ctagcctttt ctgctgacag caaacaagac gatcagacag gagacttgat taagtcagac
     4981 ccttcaggtc acttaactgg gatggttggc actgctctct atgtaagccc agaggtccaa
     5041 ggaagcacca aatctgcata caaccagaaa gtggatctct tcagcctggg aattatcttc
     5101 tttgagatgt cctatcaccc catggtcacg gcttcagaaa ggatctttgt tctcaaccaa
     5161 ctcagagatc ccacttcgcc taagtttcca gaagactttg acgatggaga gcatgcaaag
     5221 cagaaatcag tcatctcctg gctgttgaac cacgatccag caaaacggcc cacagccaca
     5281 gaactgctca agagtgagct gctgccccca ccccagatgg aggagtcaga gctgcatgaa
     5341 gtgctgcacc acacgctgac caacgtggat gggaaggcct accgcaccat gatggcccag
     5401 atcttctcgc agcgcatctc ccctgccatc gattacacct atgacagcga catactgaag
     5461 ggcaacttct caatccgtac agccaagatg cagcagcatg tgtgtgaaac catcatccgc
     5521 atctttaaaa gacatggagc tgttcagttg tgtactccac tactgcttcc ccgaaacaga
     5581 caaatatatg agcacaacga agctgcccta ttcatggacc acagcgggat gctggtgatg
     5641 cttccttttg acctgcggat cccttttgca agatatgtgg caagaaataa tatattgaat
     5701 ttaaaacgat actgcataga acgtgtgttc aggccgcgca agttagatcg atttcatccc
     5761 aaagaacttc tggagtgtgc atttgatatt gtcacttcta ccaccaacag ctttctgccc
     5821 actgctgaaa ttatctacac tatctatgaa atcatccaag agtttccagc acttcaggaa
     5881 agaaattaca gtatttattt gaaccatacc atgttattga aagcaatact cttacactgt
     5941 gggatcccag aagataaact cagtcaagtc tacattattc tgtatgatgc tgtgacagag
     6001 aagctgacga ggagagaagt ggaagctaaa ttttgtaatc tgtctttgtc ttctaatagt
     6061 ctgtgtcgac tctacaagtt tattgaacag aagggagatt tgcaagatct tatgccaaca
     6121 ataaattcat taataaaaca gaaaacaggt attgcacagt tggtgaagta tggcttaaaa
     6181 gacctagagg aggttgttgg actgttgaag aaactcggca tcaagttaca ggtcttgatc
     6241 aatttgggct tggtttacaa ggtgcagcag cacaatggaa tcatcttcca gtttgtggct
     6301 ttcatcaaac gaaggcaaag ggctgtacct gaaatcctcg cagctggagg cagatatgac
     6361 ctgctgattc cccagtttag agggccacaa gctctggggc cagttcccac tgccattggg
     6421 gtcagcatag ctatagacaa gatatctgct gctgtcctca acatggagga atctgttaca
     6481 ataagctctt gtgacctcct ggttgtaagt gttggccaga tgtctatgtc cagggccatc
     6541 aacctaaccc agaaactctg gacagcaggc atcacagcag aaatcatgta cgactggtca
     6601 cagtcccaag aggaattaca agagtactgc agacatcatg aaatcaccta tgtggccctt
     6661 gtctcggata aagaaggaag ccatgtcaag gttaagtctt tcgagaagga aaggcagaca
     6721 gagaagcgtg tgctggagac tgaacttgtg gaccatgtac tgcagaaact gaggactaaa
     6781 gtcactgatg aaaggaatgg cagagaagct tccgataatc ttgcagtgca aaatctgaag
     6841 gggtcatttt ctaatgcttc aggtttgttt gaaatccatg gagcaacagt ggttcccatt
     6901 gtgagtgtgc tagccccgga gaagctgtca gccagcacta ggaggcgcta tgaaactcag
     6961 gtacaaactc gacttcagac ctcccttgcc aacttacatc agaaaagcag tgaaattgaa
     7021 attctggctg tggatctacc caaagaaaca atattacagt ttttatcatt agagtgggat
     7081 gctgatgaac aggcatttaa cacaactgtg aagcagctgc tgtcacgcct gccaaagcaa
     7141 agatacctca aattagtctg tgatgaaatt tataacatca aagtagaaaa aaaggtgtct
     7201 gtgctatttc tgtacagcta tagagatgac tactacagaa tcttatttgg atcctaccca
     7261 tacgacgtac ctgattatgc aacttatcct tatgatgtac ctgattatgc tagctatcct
     7321 tatgatgtcc cagactacgc ataggcggcc gcgaaggatc tgcgatcgct ccggtgcccg
     7381 tcagtgggca gagcgcacat cgcccacagt ccccgagaag ttggggggag gggtcggcaa
     7441 ttgaacgggt gcctagagaa ggtggcgcgg ggtaaactgg gaaagtgatg tcgtgtactg
     7501 gctccgcctt tttcccgagg gtgggggaga accgtatata agtgcagtag tcgccgtgaa
     7561 cgttcttttt cgcaacgggt ttgccgccag aacacagctg aagcttcgag gggctcgcat
     7621 ctctccttca cgcgcccgcc gccctacctg aggccgccat ccacgccggt tgagtcgcgt
     7681 tctgccgcct cccgcctgtg gtgcctcctg aactgcgtcc gccgtctagg taagtttaaa
     7741 gctcaggtcg agaccgggcc tttgtccggc gctcccttgg agcctaccta gactcagccg
     7801 gctctccacg ctttgcctga ccctgcttgc tcaactctac gtctttgttt cgttttctgt
     7861 tctgcgccgt tacagatcca agctgtgacc ggcgcctacg ctagatgacc gagtacaagc
     7921 ccacggtgcg cctcgccacc cgcgacgacg tcccccgggc cgtacgcacc ctcgccgccg
     7981 cgttcgccga ctaccccgcc acgcgccaca ccgtcgaccc ggaccgccac atcgagcggg
     8041 tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg
     8101 tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg
     8161 cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg ctggccgcgc
     8221 agcaacagat ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg tggttcctgg
     8281 ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc
     8341 ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt cctggagacc tccgcgcccc
     8401 gcaacctccc cttctacgag cggctcggct tcaccgtcac cgccgacgtc gaggtgcccg
     8461 aaggaccgcg cacctggtgc atgacccgca agcccggtgc ctgagtcgac aatcaacctc
     8521 tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc
     8581 tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca
     8641 ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg
     8701 tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca
     8761 ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg
     8821 cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg
     8881 acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg
     8941 ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg
     9001 accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc
     9061 ctcagacgag tcggatctcc ctttgggccg cctccccgcc tggtaccttt aagaccaatg
     9121 acttacaagg cagctgtaga tcttagccac tttttaaaag aaaagggggg actggaaggg
     9181 ctaattcact cccaacgaaa ataagatctg ctttttgctt gtactgggtc tctctggtta
     9241 gaccagatct gagcctggga gctctctggc taactaggga acccactgct taagcctcaa
     9301 taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac
     9361 tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtag tagttcatgt
     9421 catcttatta ttcagtattt ataacttgca aagaaatgaa tatcagagag tgagaggaac
     9481 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat
     9541 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat
     9601 catgtctggc tctagctatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc
     9661 atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat
     9721 tccagaagta gtgaggaggc ttttttggag gcctagactt ttgcagagac ggcccaaatt
     9781 cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca
     9841 acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca
     9901 cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc
     9961 attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt
    10021 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
    10081 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
    10141 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
    10201 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
    10261 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
    10321 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
    10381 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
    10441 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
    10501 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
    10561 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
    10621 gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
    10681 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
    10741 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
    10801 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
    10861 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
    10921 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta
    10981 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa
    11041 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac
    11101 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa
    11161 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
    11221 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg
    11281 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag
    11341 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg
    11401 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc
    11461 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat
    11521 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata
    11581 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa
    11641 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca
    11701 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
    11761 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
    11821 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
    11881 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
    11941 ctgacgtcta agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga
    12001 ggccctttcg tctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac atgcagctcc
    12061 cggagacggt cacagcttgt ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg
    12121 cgtcagcggg tgttggcggg tgtcggggct ggcttaacta tgcggcatca gagcagattg
    12181 tactgagagt gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc
    12241 gcatcaggcg ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg
    12301 cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg
    12361 taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc aagctg
//
