LOCUS       V035568                11035 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V035568
VERSION     V035568
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2317..5061
                     /label="ENPP2(NM_006209)"
                     /note="ENPP2(NM_006209)"
                     /gene="ENPP2"
     CDS             5068..5133
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        5177..5388
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             5401..5669
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      5698..5707
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             5728..6390
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             6460..6513
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             6514..7113
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    7114..7702
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7776..8009
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    8081..8202
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      8242..8377
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(8410..8426)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8434..8450
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8458..8488)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8503..8524
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8812..9400)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9571..10431)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10432..10536)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     11010..11026
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc ctgatacgaa ctcggaattc gccaccatgg caaggaggag ctcgttccag
     2341 tcgtgtcaga taatatccct gttcactttt gccgttggag tcaatatctg cttaggattc
     2401 actgcacatc gaattaagag agcagaagga tgggaggaag gtcctcctac agtgctatca
     2461 gactccccct ggaccaacat ctccggatct tgcaagggca ggtgctttga acttcaagag
     2521 gctggacctc ctgattgtcg ctgtgacaac ttgtgtaaga gctataccag ttgctgccat
     2581 gactttgatg agctgtgttt gaagacagcc cgtggctggg agtgtactaa ggacagatgt
     2641 ggagaagtca gaaatgaaga aaatgcctgt cactgctcag aggactgctt ggccagggga
     2701 gactgctgta ccaattacca agtggtttgc aaaggagagt cgcattgggt tgatgatgac
     2761 tgtgaggaaa taaaggccgc agaatgccct gcagggtttg ttcgccctcc attaatcatc
     2821 ttctccgtgg atggcttccg tgcatcatac atgaagaaag gcagcaaagt catgcctaat
     2881 attgaaaaac taaggtcttg tggcacacac tctccctaca tgaggccggt gtacccaact
     2941 aaaacctttc ctaacttata cactttggcc actgggctat atccagaatc acatggaatt
     3001 gttggcaatt caatgtatga tcctgtattt gatgccactt ttcatctgcg agggcgagag
     3061 aaatttaatc atagatggtg gggaggtcaa ccgctatgga ttacagccac caagcaaggg
     3121 gtgaaagctg gaacattctt ttggtctgtt gtcatccctc acgagcggag aatattaacc
     3181 atattgcagt ggctcaccct gccagatcat gagaggcctt cggtctatgc cttctattct
     3241 gagcaacctg atttctctgg acacaaatat ggccctttcg gccctgagga gagtagttat
     3301 ggctcacctt ttactccggc taagagacct aagaggaaag ttgcccctaa gaggagacag
     3361 gaaagaccag ttgctcctcc aaagaaaaga agaagaaaaa tacataggat ggatcattat
     3421 gctgcggaaa ctcgtcagga caaaatgaca aatcctctga gggaaatcga caaaattgtg
     3481 gggcaattaa tggatggact gaaacaacta aaactgcatc ggtgtgtcaa cgtcatcttt
     3541 gtcggagacc atggaatgga agatgtcaca tgtgatagaa ctgagttctt gagtaattac
     3601 ctaactaatg tggatgatat tactttagtg cctggaactc taggaagaat tcgatccaaa
     3661 tttagcaaca atgctaaata tgaccccaaa gccattattg ccaatctcac gtgtaaaaaa
     3721 ccagatcagc actttaagcc ttacttgaaa cagcaccttc ccaaacgttt gcactatgcc
     3781 aacaacagaa gaattgagga tatccattta ttggtggaac gcagatggca tgttgcaagg
     3841 aaacctttgg atgtttataa gaaaccatca ggaaaatgct ttttccaggg agaccacgga
     3901 tttgataaca aggtcaacag catgcagact gtttttgtag gttatggctc aacatttaag
     3961 tacaagacta aagtgcctcc atttgaaaac attgaacttt acaatgttat gtgtgatctc
     4021 ctgggattga agccagctcc taataatggg acccatggaa gtttgaatca tctcctgcgc
     4081 actaatacct tcaggccaac catgccagag gaagttacca gacccaatta tccagggatt
     4141 atgtaccttc agtctgattt tgacctgggc tgcacttgtg atgataaggt agagccaaag
     4201 aacaagttgg atgaactcaa caaacggctt catacaaaag ggtctacaga agagagacac
     4261 ctcctctatg ggcgacctgc agtgctttat cggactagat atgatatctt atatcacact
     4321 gactttgaaa gtggttatag tgaaatattc ctaatgccac tctggacatc atatactgtt
     4381 tccaaacagg ctgaggtttc cagcgttcct gaccatctga ccagttgcgt ccggcctgat
     4441 gtccgtgttt ctccgagttt cagtcagaac tgtttggcct acaaaaatga taagcagatg
     4501 tcctacggat tcctctttcc tccttatctg agctcttcac cagaggctaa atatgatgca
     4561 ttccttgtaa ccaatatggt tccaatgtat cctgctttca aacgggtctg gaattatttc
     4621 caaagggtat tggtgaagaa atatgcttcg gaaagaaatg gagttaacgt gataagtgga
     4681 ccaatcttcg actatgacta tgatggctta catgacacag aagacaaaat aaaacagtac
     4741 gtggaaggca gttccattcc tgttccaact cactactaca gcatcatcac cagctgtctg
     4801 gatttcactc agcctgccga caagtgtgac ggccctctct ctgtgtcctc cttcatcctg
     4861 cctcaccggc ctgacaacga ggagagctgc aatagctcag aggacgaatc aaaatgggta
     4921 gaagaactca tgaagatgca cacagctagg gtgcgtgaca ttgaacatct caccagcctg
     4981 gacttcttcc gaaagaccag ccgcagctac ccagaaatcc tgacactcaa gacatacctg
     5041 catacatatg agagcgagat tggatccgac tacaaagacc atgacggtga ttataaagat
     5101 catgacatcg actacaagga tgacgatgac aagtagcggc cgcgaaggat ctgcgatcgc
     5161 tccggtgccc gtcagtgggc agagcgcaca tcgcccacag tccccgagaa gttgggggga
     5221 ggggtcggca attgaacggg tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat
     5281 gtcgtgtact ggctccgcct ttttcccgag ggtgggggag aaccgtatat aagtgcagta
     5341 gtcgccgtga acgttctttt tcgcaacggg tttgccgcca gaacacagct gaagcttcga
     5401 ggggctcgca tctctccttc acgcgcccgc cgccctacct gaggccgcca tccacgccgg
     5461 ttgagtcgcg ttctgccgcc tcccgcctgt ggtgcctcct gaactgcgtc cgccgtctag
     5521 gtaagtttaa agctcaggtc gagaccgggc ctttgtccgg cgctcccttg gagcctacct
     5581 agactcagcc ggctctccac gctttgcctg accctgcttg ctcaactcta cgtctttgtt
     5641 tcgttttctg ttctgcgccg ttacagatcc aagctgtgac cggcgcctac gctagacgcc
     5701 accatggaga gcgacgagag cggcctgccc gccatggaga tcgagtgccg catcaccggc
     5761 accctgaacg gcgtggagtt cgagctggtg ggcggcggag agggcacccc caagcagggc
     5821 cgcatgacca acaagatgaa gagcaccaaa ggcgccctga ccttcagccc ctacctgctg
     5881 agccacgtga tgggctacgg cttctaccac ttcggcacct accccagcgg ctacgagaac
     5941 cccttcctgc acgccatcaa caacggcggc tacaccaaca cccgcatcga gaagtacgag
     6001 gacggcggcg tgctgcacgt gagcttcagc taccgctacg aggccggccg cgtgatcggc
     6061 gacttcaagg tggtgggcac cggcttcccc gaggacagcg tgatcttcac cgacaagatc
     6121 atccgcagca acgccaccgt ggagcacctg caccccatgg gcgataacgt gctggtgggc
     6181 agcttcgccc gcaccttcag cctgcgcgac ggcggctact acagcttcgt ggtggacagc
     6241 cacatgcact tcaagagcgc catccacccc agcatcctgc agaacggggg ccccatgttc
     6301 gccttccgcc gcgtggagga gctgcacagc aacaccgagc tgggcatcgt ggagtaccag
     6361 cacgccttca agacccccat cgccttcgcc agatcccgcg ctcagtcgtc caattctgcc
     6421 gtggacggca ccgccggacc cggctccacc ggatctcgcg agggcagagg aagtcttcta
     6481 acatgcggtg acgtggagga gaatcccggc cctatgaccg agtacaagcc cacggtgcgc
     6541 ctcgccaccc gcgacgacgt ccccagggcc gtacgcaccc tcgccgccgc gttcgccgac
     6601 taccccgcca cgcgccacac cgtcgatccg gaccgccaca tcgagcgggt caccgagctg
     6661 caagaactct tcctcacgcg cgtcgggctc gacatcggca aggtgtgggt cgcggacgac
     6721 ggcgccgcgg tggcggtctg gaccacgccg gagagcgtcg aagcgggggc ggtgttcgcc
     6781 gagatcggcc cgcgcatggc cgagttgagc ggttcccggc tggccgcgca gcaacagatg
     6841 gaaggcctcc tggcgccgca ccggcccaag gagcccgcgt ggttcctggc caccgtcggc
     6901 gtctcgcccg accaccaggg caagggtctg ggcagcgccg tcgtgctccc cggagtggag
     6961 gcggccgagc gcgccggggt gcccgccttc ctggagacct ccgcgccccg caacctcccc
     7021 ttctacgagc ggctcggctt caccgtcacc gccgacgtcg aggtgcccga aggaccgcgc
     7081 acctggtgca tgacccgcaa gcccggtgcc tgaaatcaac ctctggatta caaaatttgt
     7141 gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct
     7201 ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat
     7261 aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg
     7321 gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag
     7381 ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc
     7441 tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg
     7501 tcggggaaat catcgtcctt tccttggctg ctcgcctgtg ttgccacctg gattctgcgc
     7561 gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc
     7621 ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc
     7681 tccctttggg ccgcctcccc gcctggtacc tttaagacca atgacttaca aggcagctgt
     7741 agatcttagc cactttttaa aagaaaaggg gggactggaa gggctaattc actcccaacg
     7801 aagataagat ctgctttttg cttgtactgg gtctctctgg ttagaccaga tctgagcctg
     7861 ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt
     7921 gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc
     7981 cttttagtca gtgtggaaaa tctctagcag tagtagttca tgtcatctta ttattcagta
     8041 tttataactt gcaaagaaat gaatatcaga gagtgagagg aacttgttta ttgcagctta
     8101 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact
     8161 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggctctagct
     8221 atcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg
     8281 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag
     8341 ctattccaga agtagtgagg aggctttttt ggaggcctag acttttgcag agaccaaatt
     8401 cgtaatcatg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa
     8461 catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac
     8521 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca
     8581 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc
     8641 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
     8701 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
     8761 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
     8821 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
     8881 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
     8941 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
     9001 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
     9061 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
     9121 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
     9181 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
     9241 ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
     9301 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
     9361 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
     9421 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
     9481 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta
     9541 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat
     9601 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac
     9661 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg
     9721 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag
     9781 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt
     9841 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt
     9901 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt
     9961 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt
    10021 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct
    10081 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt
    10141 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac
    10201 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa
    10261 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa
    10321 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca
    10381 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct
    10441 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga
    10501 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc
    10561 tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag
    10621 gccctttcgt ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca tgcagctccc
    10681 ggagacggtc acagcttgtc tgtaagcgga tgccgggagc agacaagccc gtcagggcgc
    10741 gtcagcgggt gttggcgggt gtcggggctg gcttaactat gcggcatcag agcagattgt
    10801 actgagagtg caccatatgc ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg
    10861 catcaggcgc cattcgccat tcaggctgcg caactgttgg gaagggcgat cggtgcgggc
    10921 ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat taagttgggt
    10981 aacgccaggg ttttcccagt cacgacgttg taaaacgacg gccagtgcca agctg
//
