LOCUS       V025079                11201 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V025079
VERSION     V025079
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2317..5244
                     /label="EPHA2(NM_004431)"
                     /note="EPHA2(NM_004431)"
                     /gene="EPHA2"
     CDS             5251..5316
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        5360..5571
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             5584..5852
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      5881..5890
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             5911..6573
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             6643..6696
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             6697..7296
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    7297..7884
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7958..8191
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    8263..8384
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      8403..8538
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(8576..8592)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8600..8616
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8624..8654)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8669..8690
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8978..9566)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9737..10597)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10598..10702)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     11176..11192
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc ctgatacgaa ctcggaattc gccaccatgg agctccaggc agcccgcgcc
     2341 tgcttcgccc tgctgtgggg ctgtgcgctg gccgcggccg cggcggcgca gggcaaggaa
     2401 gtggtactgc tggactttgc tgcagctgga ggggagctcg gctggctcac acacccgtat
     2461 ggcaaagggt gggacctgat gcagaacatc atgaatgaca tgccgatcta catgtactcc
     2521 gtgtgcaacg tgatgtctgg cgaccaggac aactggctcc gcaccaactg ggtgtaccga
     2581 ggagaggctg agcgtatctt cattgagctc aagtttactg tacgtgactg caacagcttc
     2641 cctggtggcg ccagctcctg caaggagact ttcaacctct actatgccga gtcggacctg
     2701 gactacggca ccaacttcca gaagcgcctg ttcaccaaga ttgacaccat tgcgcccgat
     2761 gagatcaccg tcagcagcga cttcgaggca cgccacgtga agctgaacgt ggaggagcgc
     2821 tccgtggggc cgctcacccg caaaggcttc tacctggcct tccaggatat cggtgcctgt
     2881 gtggcgctgc tctccgtccg tgtctactac aagaagtgcc ccgagctgct gcagggcctg
     2941 gcccacttcc ctgagaccat cgccggctct gatgcacctt ccctggccac tgtggccggc
     3001 acctgtgtgg accatgccgt ggtgccaccg gggggtgaag agccccgtat gcactgtgca
     3061 gtggatggcg agtggctggt gcccattggg cagtgcctgt gccaggcagg ctacgagaag
     3121 gtggaggatg cctgccaggc ctgctcgcct ggatttttta agtttgaggc atctgagagc
     3181 ccctgcttgg agtgccctga gcacacgctg ccatcccctg agggtgccac ctcctgcgag
     3241 tgtgaggaag gcttcttccg ggcacctcag gacccagcgt cgatgccttg cacacgaccc
     3301 ccctccgccc cacactacct cacagccgtg ggcatgggtg ccaaggtgga gctgcgctgg
     3361 acgccccctc aggacagcgg gggccgcgag gacattgtct acagcgtcac ctgcgaacag
     3421 tgctggcccg agtctgggga atgcgggccg tgtgaggcca gtgtgcgcta ctcggagcct
     3481 cctcacggac tgacccgcac cagtgtgaca gtgagcgacc tggagcccca catgaactac
     3541 accttcaccg tggaggcccg caatggcgtc tcaggcctgg taaccagccg cagcttccgt
     3601 actgccagtg tcagcatcaa ccagacagag ccccccaagg tgaggctgga gggccgcagc
     3661 accacctcgc ttagcgtctc ctggagcatc cccccgccgc agcagagccg agtgtggaag
     3721 tacgaggtca cttaccgcaa gaagggagac tccaacagct acaatgtgcg ccgcaccgag
     3781 ggtttctccg tgaccctgga cgacctggcc ccagacacca cctacctggt ccaggtgcag
     3841 gcactgacgc aggagggcca gggggccggc agcaaggtgc acgaattcca gacgctgtcc
     3901 ccggagggat ctggcaactt ggcggtgatt ggcggcgtgg ctgtcggtgt ggtcctgctt
     3961 ctggtgctgg caggagttgg cttctttatc caccgcagga ggaagaacca gcgtgcccgc
     4021 cagtccccgg aggacgttta cttctccaag tcagaacaac tgaagcccct gaagacatac
     4081 gtggaccccc acacatatga ggaccccaac caggctgtgt tgaagttcac taccgagatc
     4141 catccatcct gtgtcactcg gcagaaggtg atcggagcag gagagtttgg ggaggtgtac
     4201 aagggcatgc tgaagacatc ctcggggaag aaggaggtgc cggtggccat caagacgctg
     4261 aaagccggct acacagagaa gcagcgagtg gacttcctcg gcgaggccgg catcatgggc
     4321 cagttcagcc accacaacat catccgccta gagggcgtca tctccaaata caagcccatg
     4381 atgatcatca ctgagtacat ggagaatggg gccctggaca agttccttcg ggagaaggat
     4441 ggcgagttca gcgtgctgca gctggtgggc atgctgcggg gcatcgcagc tggcatgaag
     4501 tacctggcca acatgaacta tgtgcaccgt gacctggctg cccgcaacat cctcgtcaac
     4561 agcaacctgg tctgcaaggt gtctgacttt ggcctgtccc gcgtgctgga ggacgacccc
     4621 gaggccacct acaccaccag tggcggcaag atccccatcc gctggaccgc cccggaggcc
     4681 atttcctacc ggaagttcac ctctgccagc gacgtgtgga gctttggcat tgtcatgtgg
     4741 gaggtgatga cctatggcga gcggccctac tgggagttgt ccaaccacga ggtgatgaaa
     4801 gccatcaatg atggcttccg gctccccaca cccatggact gcccctccgc catctaccag
     4861 ctcatgatgc agtgctggca gcaggagcgt gcccgccgcc ccaagttcgc tgacatcgtc
     4921 agcatcctgg acaagctcat tcgtgcccct gactccctca agaccctggc tgactttgac
     4981 ccccgcgtgt ctatccggct ccccagcacg agcggctcgg agggggtgcc cttccgcacg
     5041 gtgtccgagt ggctggagtc catcaagatg cagcagtata cggagcactt catggcggcc
     5101 ggctacactg ccatcgagaa ggtggtgcag atgaccaacg acgacatcaa gaggattggg
     5161 gtgcggctgc ccggccacca gaagcgcatc gcctacagcc tgctgggact caaggaccag
     5221 gtgaacactg tggggatccc catcggatcc gactacaaag accatgacgg tgattataaa
     5281 gatcatgaca tcgactacaa ggatgacgat gacaagtagc ggccgcgaag gatctgcgat
     5341 cgctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
     5401 ggaggggtcg gcaattgaac gggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
     5461 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
     5521 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca gctgaagctt
     5581 cgaggggctc gcatctctcc ttcacgcgcc cgccgcccta cctgaggccg ccatccacgc
     5641 cggttgagtc gcgttctgcc gcctcccgcc tgtggtgcct cctgaactgc gtccgccgtc
     5701 taggtaagtt taaagctcag gtcgagaccg ggcctttgtc cggcgctccc ttggagccta
     5761 cctagactca gccggctctc cacgctttgc ctgaccctgc ttgctcaact ctacgtcttt
     5821 gtttcgtttt ctgttctgcg ccgttacaga tccaagctgt gaccggcgcc tacgctagac
     5881 gccaccatgg agagcgacga gagcggcctg cccgccatgg agatcgagtg ccgcatcacc
     5941 ggcaccctga acggcgtgga gttcgagctg gtgggcggcg gagagggcac ccccaagcag
     6001 ggccgcatga ccaacaagat gaagagcacc aaaggcgccc tgaccttcag cccctacctg
     6061 ctgagccacg tgatgggcta cggcttctac cacttcggca cctaccccag cggctacgag
     6121 aaccccttcc tgcacgccat caacaacggc ggctacacca acacccgcat cgagaagtac
     6181 gaggacggcg gcgtgctgca cgtgagcttc agctaccgct acgaggccgg ccgcgtgatc
     6241 ggcgacttca aggtggtggg caccggcttc cccgaggaca gcgtgatctt caccgacaag
     6301 atcatccgca gcaacgccac cgtggagcac ctgcacccca tgggcgataa cgtgctggtg
     6361 ggcagcttcg cccgcacctt cagcctgcgc gacggcggct actacagctt cgtggtggac
     6421 agccacatgc acttcaagag cgccatccac cccagcatcc tgcagaacgg gggccccatg
     6481 ttcgccttcc gccgcgtgga ggagctgcac agcaacaccg agctgggcat cgtggagtac
     6541 cagcacgcct tcaagacccc catcgccttc gccagatccc gcgctcagtc gtccaattct
     6601 gccgtggacg gcaccgccgg acccggctcc accggatctc gcgagggcag aggaagtctt
     6661 ctaacatgcg gtgacgtgga ggagaatccc ggccctatga ccgagtacaa gcccacggtg
     6721 cgcctcgcca cccgcgacga cgtccccagg gccgtacgca ccctcgccgc cgcgttcgcc
     6781 gactaccccg ccacgcgcca caccgtcgat ccggaccgcc acatcgagcg ggtcaccgag
     6841 ctgcaagaac tcttcctcac gcgcgtcggg ctcgacatcg gcaaggtgtg ggtcgcggac
     6901 gacggcgccg cggtggcggt ctggaccacg ccggagagcg tcgaagcggg ggcggtgttc
     6961 gccgagatcg gcccgcgcat ggccgagttg agcggttccc ggctggccgc gcagcaacag
     7021 atggaaggcc tcctggcgcc gcaccggccc aaggagcccg cgtggttcct ggccaccgtc
     7081 ggcgtctcgc ccgaccacca gggcaagggt ctgggcagcg ccgtcgtgct ccccggagtg
     7141 gaggcggccg agcgcgccgg ggtgcccgcc ttcctggaga cctccgcgcc ccgcaacctc
     7201 cccttctacg agcggctcgg cttcaccgtc accgccgacg tcgaggtgcc cgaaggaccg
     7261 cgcacctggt gcatgacccg caagcccggt gcctgaaatc aacctctgga ttacaaaatt
     7321 tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct
     7381 gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg
     7441 tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc
     7501 gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt
     7561 cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc
     7621 gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg
     7681 ttgtcgggga agctgacgtc ctttccatgg ctgctcgcct gtgttgccac ctggattctg
     7741 cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc
     7801 ggcctgctgc cggctctgcg gcctcttccg cgtctccgcc ttcgccctca gacgagtcgg
     7861 atctcccttt ggccgcctcc ccgcctggta cctttaagac caatgactta caaggcagct
     7921 gtagatctta gccacttttt aaaagaaaag gggggactgg aagggctaat tcactcccaa
     7981 cgaaaataag atctgctttt tgcttgtact gggtctctct ggttagacca gatctgagcc
     8041 tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga
     8101 gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga
     8161 cccttttagt cagtgtggaa aatctctagc agtagtagtt catgtcatct tattattcag
     8221 tatttataac ttgcaaagaa atgaatatca gagagtgaga ggaacttgtt tattgcagct
     8281 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca
     8341 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggctctag
     8401 ctatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt
     8461 tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag
     8521 gaggcttttt tggaggccta gacttttgca gagacggccc aaattcgtaa tcatggtcat
     8581 agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa
     8641 gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc
     8701 gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc
     8761 aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact
     8821 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac
     8881 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
     8941 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
     9001 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
     9061 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
     9121 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
     9181 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
     9241 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
     9301 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
     9361 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga
     9421 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
     9481 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
     9541 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
     9601 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
     9661 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
     9721 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc
     9781 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg
     9841 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag
     9901 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt
     9961 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag
    10021 ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt
    10081 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca
    10141 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg
    10201 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat
    10261 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta
    10321 tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca
    10381 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct
    10441 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat
    10501 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa
    10561 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt
    10621 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa
    10681 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac gtctaagaaa
    10741 ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc tttcgtctcg
    10801 cgcgtttcgg tgatgacggt gaaaacctct gacacatgca gctcccggag acggtcacag
    10861 cttgtctgta agcggatgcc gggagcagac aagcccgtca gggcgcgtca gcgggtgttg
    10921 gcgggtgtcg gggctggctt aactatgcgg catcagagca gattgtactg agagtgcacc
    10981 atatgcggtg tgaaataccg cacagatgcg taaggagaaa ataccgcatc aggcgccatt
    11041 cgccattcag gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct tcgctattac
    11101 gccagctggc gaaaggggga tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt
    11161 cccagtcacg acgttgtaaa acgacggcca gtgccaagct g
//
