LOCUS       V028814                10864 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V028814
VERSION     V028814
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2317..4890
                     /label="Eef2(NM_007907)"
                     /note="Eef2(NM_007907)"
                     /gene="Eef2"
     CDS             4897..4962
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        5006..5217
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             5230..5498
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      5527..5536
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             5557..6219
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             6289..6342
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             6343..6942
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    6943..7531
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7605..7838
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    7910..8031
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      8071..8206
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(8239..8255)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8263..8279
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8287..8317)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8332..8353
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8641..9229)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9400..10260)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10261..10365)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     10839..10855
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc ctgatacgaa ctcggaattc gccaccatgg tgaacttcac agtagatcag
     2341 atccgtgcca tcatggacaa gaaagccaac atccggaaca tgtcagtcat cgcccatgtg
     2401 gaccacggca agtccacgct gaccgactcc cttgtgtgca aggctggcat cattgcctct
     2461 gcccgagctg gggagacgcg cttcactgac actcgcaagg atgagcagga gcgctgcatc
     2521 acaatcaaat ccaccgccat ctccctcttc tacgagctct ctgagaacga cctgaacttc
     2581 attaagcaga gcaaggatgg ctcgggcttc ctcatcaacc tcatcgactc tccaggccat
     2641 gtggacttct cttcagaggt gacagctgcc ttgcgtgtca ccgatggagc tctggtggtg
     2701 gtggactgtg tgtctggcgt gtgtgtgcag acagaaactg tgctgcgcca ggccatcgct
     2761 gaacgcatca agcccgtcct gatgatgaac aagatggacc gggccctgct ggagctgcag
     2821 ctggagcccg aggagctcta ccagaccttc cagcgcatcg tggagaacgt caacgtcatc
     2881 atctctacct acggcgaggg cgagagtggg cccatgggca atatcatgat tgaccccgtc
     2941 ctgggcaccg taggctttgg ttctggcctg catggctggg ccttcaccct gaagcagttt
     3001 gcggagatgt atgtggccaa gtttgcagcc aagggtgagg gccagctgag cgcagccgag
     3061 cgtgccaaga aagtagagga catgatgaag aagctgtggg gagaccggta ctttgatccg
     3121 gccaatggca agttcagtaa gtcagccaat agcccagatg ggaaaaaact tccccgcacc
     3181 ttctgccagc tcatcctgga ccccatcttc aaggtgttcg acgccatcat gaacttcagg
     3241 aaggaggaga cagccaagct gatcgagaag ctggacatca agctggacag cgaggacaaa
     3301 gacaaggagg gcaagccatt gctcaaggct gtgatgcgcc gctggctgcc tgcaggggac
     3361 gccctcctgc agatgatcac catccactta ccatcccccg tcactgcaca gaagtaccgt
     3421 tgtgagctgc tatacgaggg gcctcccgac gatgaggccg ccatgggtat taagagctgc
     3481 gaccccaaag gcccacttat gatgtacatt tccaagatgg tgccaacctc tgacaaaggc
     3541 cgcttctatg cctttggtag agtgttctct ggggtggtgt ccacgggcct gaaggtccgg
     3601 atcatgggcc ccaactacac gcctgggaag aaagaggacc tatacctgaa gcctatccag
     3661 agaaccattc tgatgatggg ccgctacgtg gagccgattg aggacgtgcc atgtggaaac
     3721 attgtggggc tggtcggagt ggaccagttc cttgtgaaga cggggaccat cactaccttt
     3781 gagcacgctc acaacatgcg cgtgatgaag ttcagcgtca gccctgtcgt ccgtgtggca
     3841 gtggaggcca agaacccagc tgacctgccc aagctggtgg aggggctgaa gcggctggct
     3901 aagtctgacc ctatggtgca gtgcatcatt gaggagtctg gagaacatat tattgctggc
     3961 gccggtgagc tgcacctgga gatctgcctt aaggacctgg aggaggacca tgcctgcatc
     4021 cccatcaaga aatctgaccc tgttgtgtca tatcgggaga cagtcagtga ggaatccaac
     4081 gtgctgtgtc tgtccaagtc ccccaataag cacaaccggc tgtacatgaa ggccaggccc
     4141 ttccctgatg gcctggcaga ggacatcgat aagggtgagg tgtctgcccg ccaggagctc
     4201 aaggcacgtg cccgctacct ggccgaaaag tatgagtggg acgttgctga agcccgcaag
     4261 atctggtgct ttggccctga tggcactggc cccaacattc tcaccgacat caccaagggt
     4321 gtgcagtacc tgaatgagat caaggacagt gtggtggctg gcttccagtg ggctactaag
     4381 gagggcgctc tctgtgagga aaacatgcgt ggtgtgcggt ttgatgttca tgatgtgacc
     4441 ctgcatgctg atgccattca ccggggaggt ggccagatca tccccacagc acgccgctgc
     4501 ctgtatgcca gtgtgctgac cgcacagccc cgcctcatgg agcctatcta tctggtggag
     4561 atccagtgtc ctgagcaagt ggtgggtggc atctacggtg tcctgaacag gaagcgtggc
     4621 catgtgtttg aggagtccca ggtggctggt acccccatgt ttgtggtcaa ggcatacctg
     4681 cctgtcaatg agtcctttgg cttcaccgct gatctgcgat ccaacaccgg cggccaggcc
     4741 ttcccccagt gcgtgtttga ccactggcag atcctgcctg gggatccttt tgacaacagc
     4801 agccgcccca gccaagtggt agctgagacg cgcaagcgca agggcctgaa agagggcatc
     4861 ccagcgctgg acaacttcct ggacaaactg ggatccgact acaaagacca tgacggtgat
     4921 tataaagatc atgacatcga ctacaaggat gacgatgaca agtagcggcc gcgaaggatc
     4981 tgcgatcgct ccggtgcccg tcagtgggca gagcgcacat cgcccacagt ccccgagaag
     5041 ttggggggag gggtcggcaa ttgaacgggt gcctagagaa ggtggcgcgg ggtaaactgg
     5101 gaaagtgatg tcgtgtactg gctccgcctt tttcccgagg gtgggggaga accgtatata
     5161 agtgcagtag tcgccgtgaa cgttcttttt cgcaacgggt ttgccgccag aacacagctg
     5221 aagcttcgag gggctcgcat ctctccttca cgcgcccgcc gccctacctg aggccgccat
     5281 ccacgccggt tgagtcgcgt tctgccgcct cccgcctgtg gtgcctcctg aactgcgtcc
     5341 gccgtctagg taagtttaaa gctcaggtcg agaccgggcc tttgtccggc gctcccttgg
     5401 agcctaccta gactcagccg gctctccacg ctttgcctga ccctgcttgc tcaactctac
     5461 gtctttgttt cgttttctgt tctgcgccgt tacagatcca agctgtgacc ggcgcctacg
     5521 ctagacgcca ccatggagag cgacgagagc ggcctgcccg ccatggagat cgagtgccgc
     5581 atcaccggca ccctgaacgg cgtggagttc gagctggtgg gcggcggaga gggcaccccc
     5641 aagcagggcc gcatgaccaa caagatgaag agcaccaaag gcgccctgac cttcagcccc
     5701 tacctgctga gccacgtgat gggctacggc ttctaccact tcggcaccta ccccagcggc
     5761 tacgagaacc ccttcctgca cgccatcaac aacggcggct acaccaacac ccgcatcgag
     5821 aagtacgagg acggcggcgt gctgcacgtg agcttcagct accgctacga ggccggccgc
     5881 gtgatcggcg acttcaaggt ggtgggcacc ggcttccccg aggacagcgt gatcttcacc
     5941 gacaagatca tccgcagcaa cgccaccgtg gagcacctgc accccatggg cgataacgtg
     6001 ctggtgggca gcttcgcccg caccttcagc ctgcgcgacg gcggctacta cagcttcgtg
     6061 gtggacagcc acatgcactt caagagcgcc atccacccca gcatcctgca gaacgggggc
     6121 cccatgttcg ccttccgccg cgtggaggag ctgcacagca acaccgagct gggcatcgtg
     6181 gagtaccagc acgccttcaa gacccccatc gccttcgcca gatcccgcgc tcagtcgtcc
     6241 aattctgccg tggacggcac cgccggaccc ggctccaccg gatctcgcga gggcagagga
     6301 agtcttctaa catgcggtga cgtggaggag aatcccggcc ctatgaccga gtacaagccc
     6361 acggtgcgcc tcgccacccg cgacgacgtc cccagggccg tacgcaccct cgccgccgcg
     6421 ttcgccgact accccgccac gcgccacacc gtcgatccgg accgccacat cgagcgggtc
     6481 accgagctgc aagaactctt cctcacgcgc gtcgggctcg acatcggcaa ggtgtgggtc
     6541 gcggacgacg gcgccgcggt ggcggtctgg accacgccgg agagcgtcga agcgggggcg
     6601 gtgttcgccg agatcggccc gcgcatggcc gagttgagcg gttcccggct ggccgcgcag
     6661 caacagatgg aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg gttcctggcc
     6721 accgtcggcg tctcgcccga ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc
     6781 ggagtggagg cggccgagcg cgccggggtg cccgccttcc tggagacctc cgcgccccgc
     6841 aacctcccct tctacgagcg gctcggcttc accgtcaccg ccgacgtcga ggtgcccgaa
     6901 ggaccgcgca cctggtgcat gacccgcaag cccggtgcct gaaatcaacc tctggattac
     6961 aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga
     7021 tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc
     7081 tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa
     7141 cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc
     7201 acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc
     7261 atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc
     7321 gtggtgttgt cggggaaatc atcgtccttt ccttggctgc tcgcctgtgt tgccacctgg
     7381 attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct
     7441 tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg
     7501 agtcggatct ccctttgggc cgcctccccg cctggtacct ttaagaccaa tgacttacaa
     7561 ggcagctgta gatcttagcc actttttaaa agaaaagggg ggactggaag ggctaattca
     7621 ctcccaacga agataagatc tgctttttgc ttgtactggg tctctctggt tagaccagat
     7681 ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt
     7741 gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc
     7801 cctcagaccc ttttagtcag tgtggaaaat ctctagcagt agtagttcat gtcatcttat
     7861 tattcagtat ttataacttg caaagaaatg aatatcagag agtgagagga acttgtttat
     7921 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
     7981 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg
     8041 gctctagcta tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc
     8101 cattctccgc cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg
     8161 gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctaga cttttgcaga
     8221 gaccaaattc gtaatcatgt catagctgtt tcctgtgtga aattgttatc cgctcacaat
     8281 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag
     8341 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg
     8401 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc
     8461 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
     8521 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
     8581 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     8641 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     8701 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     8761 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     8821 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     8881 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     8941 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     9001 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     9061 taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac
     9121 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
     9181 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     9241 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
     9301 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
     9361 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
     9421 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
     9481 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
     9541 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
     9601 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
     9661 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
     9721 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
     9781 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
     9841 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
     9901 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
     9961 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
    10021 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
    10081 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
    10141 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
    10201 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
    10261 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
    10321 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
    10381 agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg
    10441 tatcacgagg ccctttcgtc tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat
    10501 gcagctcccg gagacggtca cagcttgtct gtaagcggat gccgggagca gacaagcccg
    10561 tcagggcgcg tcagcgggtg ttggcgggtg tcggggctgg cttaactatg cggcatcaga
    10621 gcagattgta ctgagagtgc accatatgcg gtgtgaaata ccgcacagat gcgtaaggag
    10681 aaaataccgc atcaggcgcc attcgccatt caggctgcgc aactgttggg aagggcgatc
    10741 ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt
    10801 aagttgggta acgccagggt tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa
    10861 gctg
//
