LOCUS       V024683                11903 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V024683
VERSION     V024683
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2317..5946
                     /label="Egfr(NM_207655)"
                     /note="Egfr(NM_207655)"
                     /gene="Egfr"
     CDS             5953..6018
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        6062..6273
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             6286..6554
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      6583..6592
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             6613..7275
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             7345..7398
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             7399..7998
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    7999..8586
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             8660..8893
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    8965..9086
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      9105..9240
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(9278..9294)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    9302..9318
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(9326..9356)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    9371..9392
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(9680..10268)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(10439..11299)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(11300..11404)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     11878..11894
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc ctgatacgaa ctcggaattc gccaccatgc gaccctcagg gaccgcgaga
     2341 accacactgc tggtgttgct gaccgcgctc tgcgccgcag gtggggcgtt ggaggaaaag
     2401 aaagtctgcc aaggcacaag taacaggctc acccaactgg gcacttttga agaccacttt
     2461 ctgagcctgc agaggatgta caacaactgt gaagtggtcc ttgggaactt ggaaattacc
     2521 tatgtgcaaa ggaattacga cctttccttc ttaaagacca tccaggaggt ggccggctat
     2581 gtcctcattg ccctcaacac cgtggagaga atccctttgg agaacctgca gatcatcagg
     2641 ggaaatgctc tttatgaaaa cacctatgcc ttagccatcc tgtccaacta tgggacaaac
     2701 agaactgggc ttagggaact gcccatgcgg aacttacagg aaatcctgat tggtgctgtg
     2761 cgattcagca acaaccccat cctctgcaat atggatacta tccagtggag ggacatcgtc
     2821 caaaacgtct ttatgagcaa catgtcaatg gacttacaga gccatccgag cagttgcccc
     2881 aaatgtgatc caagctgtcc caatggaagc tgctggggag gaggagagga gaactgccag
     2941 aaattgacca aaatcatctg tgcccagcaa tgttcccatc gctgtcgtgg caggtccccc
     3001 agtgactgct gccacaacca atgtgctgcg gggtgtacag ggccccgaga gagtgactgt
     3061 ctggtctgcc aaaagttcca agatgaggcc acatgcaaag acacctgccc accactcatg
     3121 ctgtacaacc ccaccaccta tcagatggat gtcaaccctg aagggaagta cagctttggt
     3181 gccacctgtg tgaagaagtg cccccgaaac tacgtggtga cagatcatgg ctcatgtgtc
     3241 cgagcctgtg ggcctgacta ctacgaagtg gaagaagatg gcatccgcaa gtgtaaaaaa
     3301 tgtgatgggc cctgtcgcaa agtttgtaat ggcataggca ttggtgaatt taaagacaca
     3361 ctctccataa atgctacaaa catcaaacac ttcaaatact gcactgccat cagcggggac
     3421 cttcacatcc tgccagtggc ctttaagggg gattctttca cgcgcactcc tcctctagac
     3481 ccacgagaac tagaaattct aaaaaccgta aaggaaataa caggcttttt gctgattcag
     3541 gcttggcctg ataactggac tgacctccat gctttcgaga acctagaaat aatacgtggc
     3601 agaacaaagc aacatggtca gttttctttg gcggtcgttg gcctgaacat cacatcactg
     3661 gggctgcgtt ccctcaagga gatcagtgat ggggatgtga tcatttctgg aaaccgaaat
     3721 ttgtgctacg caaacacaat aaactggaaa aaactcttcg ggacacccaa tcagaaaacc
     3781 aaaatcatga acaacagagc tgagaaagac tgcaaggccg tgaaccacgt ctgcaatcct
     3841 ttatgctcct cggaaggctg ctggggccct gagcccaggg actgtgtctc ctgccagaat
     3901 gtgagcagag gcagggagtg cgtggagaaa tgcaacatcc tggaggggga accaagggag
     3961 tttgtggaaa attctgaatg catccagtgc catccagaat gtctgcccca ggccatgaac
     4021 atcacctgta caggcagggg accagacaac tgcatccagt gtgcccacta cattgatggc
     4081 ccacactgtg tcaagacctg cccagctggc atcatgggag agaacaacac tctggtctgg
     4141 aagtatgcag atgccaataa tgtctgccac ctatgccacg ccaactgtac ctatggatgt
     4201 gctgggccag gtcttcaagg atgtgaagtg tggccatctg ggccaaagat accatctatt
     4261 gccactggga ttgtgggtgg cctcctcttc atagtggtgg tggcccttgg gattggccta
     4321 ttcatgcgaa gacgtcacat tgttcgaaag cgtacactac gccgcctgct tcaagagaga
     4381 gagctcgtgg aacctctcac acccagcgga gaagctccaa accaagccca cttgaggata
     4441 ttaaaggaaa cagaattcaa aaagatcaaa gttctgggtt cgggagcatt tggcacagtg
     4501 tataagggtc tctggatccc agaaggtgag aaagtaaaaa tcccggtggc catcaaggag
     4561 ttaagagaag ccacatctcc aaaagccaac aaagaaatcc ttgacgaagc ctatgtgatg
     4621 gctagtgtgg acaaccctca tgtatgccgc ctcctgggca tctgtctgac ctccactgtc
     4681 cagctcatta cacagctcat gccctacggt tgcctcctgg actacgtccg agaacacaag
     4741 gacaacattg gctcccagta cctcctcaac tggtgtgtgc agattgcaaa gggcatgaac
     4801 tacctggaag atcggcgttt ggtgcaccgt gacttggcag ccaggaatgt actggtgaag
     4861 acaccacagc atgtcaagat cacagatttt gggctggcca aactgcttgg tgctgaagag
     4921 aaagaatatc atgccgaggg gggcaaagtg cctatcaagt ggatggcttt ggaatcaatt
     4981 ttacaccgaa tttatacaca ccaaagtgat gtctggagct atggtgtcac tgtgtgggaa
     5041 ctgatgacct ttgggtccaa gccttatgat ggaatcccag caagtgacat ctcatccatc
     5101 ctagagaaag gagagcgcct tccacagcca cctatctgca ccatcgatgt ctacatgatc
     5161 atggtcaagt gctggatgat agatgctgat agccgcccaa agttccgaga gttgattctt
     5221 gaattctcca aaatggcccg agacccacag cgctaccttg ttatccaggg ggatgaaaga
     5281 atgcatttgc caagccctac agactccaac ttttaccgag ccctgatgga tgaagaggac
     5341 atggaggatg tagttgatgc tgatgagtat cttatcccac agcaaggctt cttcaacagc
     5401 ccgtccacgt cgaggactcc cctcttgagt tctctgagtg caactagcaa caattccact
     5461 gtggcttgca ttaatagaaa tgggagctgc cgtgtcaaag aagacgcctt cttgcagcgg
     5521 tacagctccg accccacagg tgctgtaaca gaggacaaca tagatgacgc attcctccct
     5581 gtacctgaat atgtaaacca atctgttccc aagaggccag caggctctgt gcagaaccct
     5641 gtctatcaca atcagcccct gcatccagct cctggaagag acctgcatta tcaaaatccc
     5701 cacagcaatg cagtgggcaa ccctgagtat ctcaacactg cccagcctac ctgtctcagt
     5761 agtgggttta acagccctgc actctggatc cagaaaggca gtcaccaaat gagcctagac
     5821 aaccctgact accagcagga cttcttcccc aaggaaacca agccaaatgg catatttaag
     5881 ggccccacag ctgaaaatgc agagtaccta cgggtggcac ctccaagcag tgagtttatt
     5941 ggagcaggat ccgactacaa agaccatgac ggtgattata aagatcatga catcgactac
     6001 aaggatgacg atgacaagta gcggccgcga aggatctgcg atcgctccgg tgcccgtcag
     6061 tgggcagagc gcacatcgcc cacagtcccc gagaagttgg ggggaggggt cggcaattga
     6121 acgggtgcct agagaaggtg gcgcggggta aactgggaaa gtgatgtcgt gtactggctc
     6181 cgcctttttc ccgagggtgg gggagaaccg tatataagtg cagtagtcgc cgtgaacgtt
     6241 ctttttcgca acgggtttgc cgccagaaca cagctgaagc ttcgaggggc tcgcatctct
     6301 ccttcacgcg cccgccgccc tacctgaggc cgccatccac gccggttgag tcgcgttctg
     6361 ccgcctcccg cctgtggtgc ctcctgaact gcgtccgccg tctaggtaag tttaaagctc
     6421 aggtcgagac cgggcctttg tccggcgctc ccttggagcc tacctagact cagccggctc
     6481 tccacgcttt gcctgaccct gcttgctcaa ctctacgtct ttgtttcgtt ttctgttctg
     6541 cgccgttaca gatccaagct gtgaccggcg cctacgctag acgccaccat ggagagcgac
     6601 gagagcggcc tgcccgccat ggagatcgag tgccgcatca ccggcaccct gaacggcgtg
     6661 gagttcgagc tggtgggcgg cggagagggc acccccaagc agggccgcat gaccaacaag
     6721 atgaagagca ccaaaggcgc cctgaccttc agcccctacc tgctgagcca cgtgatgggc
     6781 tacggcttct accacttcgg cacctacccc agcggctacg agaacccctt cctgcacgcc
     6841 atcaacaacg gcggctacac caacacccgc atcgagaagt acgaggacgg cggcgtgctg
     6901 cacgtgagct tcagctaccg ctacgaggcc ggccgcgtga tcggcgactt caaggtggtg
     6961 ggcaccggct tccccgagga cagcgtgatc ttcaccgaca agatcatccg cagcaacgcc
     7021 accgtggagc acctgcaccc catgggcgat aacgtgctgg tgggcagctt cgcccgcacc
     7081 ttcagcctgc gcgacggcgg ctactacagc ttcgtggtgg acagccacat gcacttcaag
     7141 agcgccatcc accccagcat cctgcagaac gggggcccca tgttcgcctt ccgccgcgtg
     7201 gaggagctgc acagcaacac cgagctgggc atcgtggagt accagcacgc cttcaagacc
     7261 cccatcgcct tcgccagatc ccgcgctcag tcgtccaatt ctgccgtgga cggcaccgcc
     7321 ggacccggct ccaccggatc tcgcgagggc agaggaagtc ttctaacatg cggtgacgtg
     7381 gaggagaatc ccggccctat gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac
     7441 gacgtcccca gggccgtacg caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc
     7501 cacaccgtcg atccggaccg ccacatcgag cgggtcaccg agctgcaaga actcttcctc
     7561 acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg
     7621 gtctggacca cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc
     7681 atggccgagt tgagcggttc ccggctggcc gcgcagcaac agatggaagg cctcctggcg
     7741 ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac
     7801 cagggcaagg gtctgggcag cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc
     7861 ggggtgcccg ccttcctgga gacctccgcg ccccgcaacc tccccttcta cgagcggctc
     7921 ggcttcaccg tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc
     7981 cgcaagcccg gtgcctgaaa tcaacctctg gattacaaaa tttgtgaaag attgactggt
     8041 attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat
     8101 catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg
     8161 tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt
     8221 gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact
     8281 ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc
     8341 tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaagctgacg
     8401 tcctttccat ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc
     8461 tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg
     8521 cggcctcttc cgcgtctccg ccttcgccct cagacgagtc ggatctccct ttggccgcct
     8581 ccccgcctgg tacctttaag accaatgact tacaaggcag ctgtagatct tagccacttt
     8641 ttaaaagaaa aggggggact ggaagggcta attcactccc aacgaaaata agatctgctt
     8701 tttgcttgta ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa
     8761 ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt
     8821 gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg
     8881 aaaatctcta gcagtagtag ttcatgtcat cttattattc agtatttata acttgcaaag
     8941 aaatgaatat cagagagtga gaggaacttg tttattgcag cttataatgg ttacaaataa
     9001 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt
     9061 ttgtccaaac tcatcaatgt atcttatcat gtctggctct agctatcccg cccctaactc
     9121 cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg
     9181 ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc
     9241 tagacttttg cagagacggc ccaaattcgt aatcatggtc atagctgttt cctgtgtgaa
     9301 attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct
     9361 ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc
     9421 agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg
     9481 gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
     9541 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
     9601 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
     9661 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
     9721 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
     9781 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
     9841 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
     9901 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
     9961 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
    10021 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
    10081 agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg
    10141 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
    10201 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
    10261 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
    10321 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
    10381 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
    10441 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
    10501 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
    10561 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc
    10621 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
    10681 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
    10741 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
    10801 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
    10861 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
    10921 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
    10981 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
    11041 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
    11101 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
    11161 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
    11221 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
    11281 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
    11341 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
    11401 cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga aaccattatt atcatgacat
    11461 taacctataa aaataggcgt atcacgaggc cctttcgtct cgcgcgtttc ggtgatgacg
    11521 gtgaaaacct ctgacacatg cagctcccgg agacggtcac agcttgtctg taagcggatg
    11581 ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggctggc
    11641 ttaactatgc ggcatcagag cagattgtac tgagagtgca ccatatgcgg tgtgaaatac
    11701 cgcacagatg cgtaaggaga aaataccgca tcaggcgcca ttcgccattc aggctgcgca
    11761 actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg
    11821 gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta
    11881 aaacgacggc cagtgccaag ctg
//
