LOCUS       V040623                10486 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V040623
VERSION     V040623
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2317..4512
                     /label="Elmo2(NM_207706)"
                     /note="Elmo2(NM_207706)"
                     /gene="Elmo2"
     CDS             4519..4584
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        4628..4839
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             4852..5120
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      5149..5158
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             5179..5841
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             5911..5964
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             5965..6564
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    6565..7153
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7227..7460
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    7532..7653
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      7693..7828
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(7861..7877)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    7885..7901
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(7909..7939)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    7954..7975
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8263..8851)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9022..9882)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(9883..9987)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     10461..10477
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc ctgatacgaa ctcggaattc gccaccatgc cgcctccgtc tgacattgtc
     2341 aaagtggcca tcgagtggcc aggtgctaac gcccagctcc ttgaaattga ccagaaacgg
     2401 ccgctggcat ccatcatcaa ggaggtgtgc gatgggtggt cactgccgaa cccggagtac
     2461 tacaccctcc gctatgcaga tgggcctcag ctctacgtca cggagcagac ccgaaatgac
     2521 attaagaacg ggacaatctt acaactggcc gtctccccgt cccgggctgc acgccagctg
     2581 atggaaagga cccagtcatc tagtatggag acccggctgg atgccatgaa ggagttggct
     2641 aagctctcag ctgacgtgac tttcgccacg gagttcatta acatggacgg catcattgtg
     2701 ctgacgaggc tcgtggagag tgggaccaag ctcctgtccc actacagtga gatgctggca
     2761 ttcaccctga ctgccttctt agagctcatg gatcatggca ttgtctcctg ggacatggtt
     2821 tcagtcacct ttattaagca gattgcaggg tacgtgagcc agcccatggt cgatgtgtcc
     2881 atcctccagc ggtccctggc catcctagag agcatggtac taaacagcca gagcctgtac
     2941 cagaagatag cggaggagat caccgtggga cagctcatct cccacctgca ggtctccaac
     3001 caggagatcc agacctacgc cattgctctg attaacgcgc tgttcctgaa ggcccccgaa
     3061 gacaagagac aggacaagca ccttaaccct ctagacctgc ccgtcactga catggccaat
     3121 gcctttgcac agaagcacct tcggtccata atcctgaacc atgtgatcag agggaatcgt
     3181 ccaatcaaaa cagagatggc ccatcagctg tatgtccttc aggtcttgac ctttaacctt
     3241 ctggaagaaa gaatgatgac caagatggat cccaatgacc aggctcagag agacattata
     3301 tttgaactga ggaggattgc cttcgacgca gagtctgacc ccagcaacgt ccccgggagt
     3361 gggactgaaa agcgcaaggc catgtatacc aaggactata aaatgctggg cttcaccaac
     3421 catatcaacc cagccttgga cttcacccag actcctcctg gaatgctggc gctggacaac
     3481 atgctgtacc tggctaaagt ccaccaggac acctacatcc ggatcgtgct ggagaacagc
     3541 agtcgggagg acaaacacga gtgtccgttc ggccgcagtg ccatcgagct caccaagatg
     3601 ctctgtgaga tcctgcaggt cggggagctc cctaatgaag ggcgcaatga ctaccacccc
     3661 atgttcttca cccacgaccg agccttcgag gaactcttcg ggatctgcat ccagctgctg
     3721 aacaagacct ggaaggagat gagggcgaca gccgaggatt tcaacaaggt tatgcaagtt
     3781 gtccgagagc agatcacccg ggctctgccc tctaaaccca actctttgga tcagttcaag
     3841 agtaaacttc gtagcctgag ctactcagaa attctgcggt tgcgccagtc tgagaggatg
     3901 agccaggatg atttccagtc cccaccaatt gtggagcttc gagagaagat acagcctgag
     3961 atcctggagc tgatcaagca acagcgcctc aaccggctat gcgagggcag cagcttccgg
     4021 aaaatcggga accgtcgtcg gcaagagagg ttctggcact gccgcttggc actgaaccac
     4081 aaggttttgc attacggtga cttggatgac aaccctcaag gggaggtgac attcgaatcc
     4141 ctgcaggaga aaattcctgt tgcagatatt aaggccattg ttactggaaa agactgtcct
     4201 cacatgaaag agaagagtgc cctgaaacag aacaaggagg tgttggaatt ggccttctcc
     4261 atcctgtatg atcctgatga gacactgaat ttcattgctc ccaacaagta tgagtactgc
     4321 atctggattg atggactcag cgctctcctg gggaaggata tgtccagcga gctaaccaag
     4381 agcgacttgg acacgctgct gagcatggag atgaagctgc ggcttctgga cttggagaac
     4441 attcagatcc ccgaggcgcc acctccggtc cccaaggagc ccagcagcta tgacttcgtc
     4501 tatcactatg gcggatccga ctacaaagac catgacggtg attataaaga tcatgacatc
     4561 gactacaagg atgacgatga caagtagcgg ccgcgaagga tctgcgatcg ctccggtgcc
     4621 cgtcagtggg cagagcgcac atcgcccaca gtccccgaga agttgggggg aggggtcggc
     4681 aattgaacgg gtgcctagag aaggtggcgc ggggtaaact gggaaagtga tgtcgtgtac
     4741 tggctccgcc tttttcccga gggtggggga gaaccgtata taagtgcagt agtcgccgtg
     4801 aacgttcttt ttcgcaacgg gtttgccgcc agaacacagc tgaagcttcg aggggctcgc
     4861 atctctcctt cacgcgcccg ccgccctacc tgaggccgcc atccacgccg gttgagtcgc
     4921 gttctgccgc ctcccgcctg tggtgcctcc tgaactgcgt ccgccgtcta ggtaagttta
     4981 aagctcaggt cgagaccggg cctttgtccg gcgctccctt ggagcctacc tagactcagc
     5041 cggctctcca cgctttgcct gaccctgctt gctcaactct acgtctttgt ttcgttttct
     5101 gttctgcgcc gttacagatc caagctgtga ccggcgccta cgctagacgc caccatggag
     5161 agcgacgaga gcggcctgcc cgccatggag atcgagtgcc gcatcaccgg caccctgaac
     5221 ggcgtggagt tcgagctggt gggcggcgga gagggcaccc ccaagcaggg ccgcatgacc
     5281 aacaagatga agagcaccaa aggcgccctg accttcagcc cctacctgct gagccacgtg
     5341 atgggctacg gcttctacca cttcggcacc taccccagcg gctacgagaa ccccttcctg
     5401 cacgccatca acaacggcgg ctacaccaac acccgcatcg agaagtacga ggacggcggc
     5461 gtgctgcacg tgagcttcag ctaccgctac gaggccggcc gcgtgatcgg cgacttcaag
     5521 gtggtgggca ccggcttccc cgaggacagc gtgatcttca ccgacaagat catccgcagc
     5581 aacgccaccg tggagcacct gcaccccatg ggcgataacg tgctggtggg cagcttcgcc
     5641 cgcaccttca gcctgcgcga cggcggctac tacagcttcg tggtggacag ccacatgcac
     5701 ttcaagagcg ccatccaccc cagcatcctg cagaacgggg gccccatgtt cgccttccgc
     5761 cgcgtggagg agctgcacag caacaccgag ctgggcatcg tggagtacca gcacgccttc
     5821 aagaccccca tcgccttcgc cagatcccgc gctcagtcgt ccaattctgc cgtggacggc
     5881 accgccggac ccggctccac cggatctcgc gagggcagag gaagtcttct aacatgcggt
     5941 gacgtggagg agaatcccgg ccctatgacc gagtacaagc ccacggtgcg cctcgccacc
     6001 cgcgacgacg tccccagggc cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc
     6061 acgcgccaca ccgtcgatcc ggaccgccac atcgagcggg tcaccgagct gcaagaactc
     6121 ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg tcgcggacga cggcgccgcg
     6181 gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc
     6241 ccgcgcatgg ccgagttgag cggttcccgg ctggccgcgc agcaacagat ggaaggcctc
     6301 ctggcgccgc accggcccaa ggagcccgcg tggttcctgg ccaccgtcgg cgtctcgccc
     6361 gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag
     6421 cgcgccgggg tgcccgcctt cctggagacc tccgcgcccc gcaacctccc cttctacgag
     6481 cggctcggct tcaccgtcac cgccgacgtc gaggtgcccg aaggaccgcg cacctggtgc
     6541 atgacccgca agcccggtgc ctgaaatcaa cctctggatt acaaaatttg tgaaagattg
     6601 actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct
     6661 ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg
     6721 ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact
     6781 gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc
     6841 gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc
     6901 cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa
     6961 tcatcgtcct ttccttggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc
     7021 ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg
     7081 gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg
     7141 gccgcctccc cgcctggtac ctttaagacc aatgacttac aaggcagctg tagatcttag
     7201 ccacttttta aaagaaaagg ggggactgga agggctaatt cactcccaac gaagataaga
     7261 tctgcttttt gcttgtactg ggtctctctg gttagaccag atctgagcct gggagctctc
     7321 tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt
     7381 agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc
     7441 agtgtggaaa atctctagca gtagtagttc atgtcatctt attattcagt atttataact
     7501 tgcaaagaaa tgaatatcag agagtgagag gaacttgttt attgcagctt ataatggtta
     7561 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag
     7621 ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggctctagc tatcccgccc
     7681 ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc
     7741 tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag
     7801 aagtagtgag gaggcttttt tggaggccta gacttttgca gagaccaaat tcgtaatcat
     7861 gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc
     7921 cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc
     7981 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat
     8041 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac
     8101 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
     8161 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
     8221 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
     8281 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     8341 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     8401 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
     8461 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     8521 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     8581 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
     8641 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
     8701 aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
     8761 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
     8821 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
     8881 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
     8941 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
     9001 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
     9061 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
     9121 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
     9181 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
     9241 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
     9301 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc
     9361 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
     9421 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
     9481 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
     9541 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
     9601 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca
     9661 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
     9721 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
     9781 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
     9841 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
     9901 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
     9961 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta
    10021 agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga ggccctttcg
    10081 tctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac atgcagctcc cggagacggt
    10141 cacagcttgt ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg cgtcagcggg
    10201 tgttggcggg tgtcggggct ggcttaacta tgcggcatca gagcagattg tactgagagt
    10261 gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc gcatcaggcg
    10321 ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct
    10381 attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg
    10441 gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc aagctg
//
