LOCUS V022270 12426 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V022270 VERSION V022270 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers LTR 234..414 /note="truncated 5' long terminal repeat (LTR) from HIV-1" /label="5' LTR (truncated)" misc_feature 458..583 /note="packaging signal of human immunodeficiency virus type 1" /label="HIV-1 Ψ" misc_feature 1076..1309 /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." /label="RRE" CDS 1493..1537 /codon_start=1 /note="recognized by the 2H10 single-chain llama nanobody" /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /transl_table=1 /translation="KNEQELLELDKWASL" /label="gp41 peptide" misc_feature 1804..1921 /note="central polypurine tract and central termination sequence of HIV-1" /label="cPPT/CTS" promoter 2011..2214 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" regulatory 2291..2300 /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" /regulatory_class="other" /label="Kozak sequence" CDS 2300..2323 /codon_start=1 /product="FLAG® epitope tag, followed by an enterokinase cleavage site" /transl_table=1 /translation="DYKDDDDK" /label="FLAG" CDS 2324..6541 /label="FAM135B(NM_015912)" /note="FAM135B(NM_015912)" /gene="FAM135B" promoter 6585..6796 /note="core promoter for human elongation factor EF-1α" /label="EF-1α core promoter" LTR 6809..7077 /note="truncated 5' long terminal repeat (LTR) from human T-cell leukemia virus (HTLV) type 1" /label="5' LTR (truncated)" regulatory 7106..7115 /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" /regulatory_class="other" /label="Kozak sequence" CDS 7136..7798 /codon_start=1 /product="green fluorescent protein 2 from Pontellina plumata, also known as ppluGFP2 (Shagin et al., 2004)" /transl_table=1 /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA" /label="CopGFP" CDS 7868..7921 /codon_start=1 /note="Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides." /product="2A peptide from Thosea asigna virus capsid protein" /protein_id="" /transl_table=1 /translation="EGRGSLLTCGDVEENPGP" /label="T2A" CDS 7922..8521 /codon_start=1 /gene="pac from Streptomyces alboniger" /note="confers resistance to puromycin" /product="puromycin N-acetyltransferase" /transl_table=1 /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*" /label="PuroR" misc_feature 8522..9109 /note="woodchuck hepatitis virus posttranscriptional regulatory element" /label="WPRE" LTR 9183..9416 /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" /label="3' LTR (ΔU3)" polyA_signal 9488..9609 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" rep_origin 9628..9763 /note="SV40 origin of replication" /label="SV40 ori" primer_bind complement(9801..9817) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 9825..9841 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(9849..9879) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 9894..9915 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(10203..10791) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(10962..11822) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(11823..11927) /gene="bla" /label="AmpR promoter" primer_bind 12401..12417 /note="common sequencing primer, one of multiple similar variants" /label="M13 fwd" ORIGIN 1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc gccaccatgg attacaagga tgacgacgat aagatgtctg aaatacaagg 2341 aacggttgag ttttcggtag agctacataa attttataat gtggatctct ttcagagagg 2401 gtattaccag atccgagtga ccttgaaggt gtcttcaagg atcccccaca gactgagtgc 2461 ctccatcgct gggcagacag agagcagcag cctgcattca gcctgtgtcc atgacagcac 2521 cgtgcacagc cgggtctttc agatcttata ccggaatgaa gaggtaccca taaatgatgc 2581 tgtggtcttc cgagttcatt tactcttggg tggtgaaagg atggaagacg cactgagtga 2641 agtagatttt caactcaagg tggatctgca ctttacggac agtgaacagc agttgaggga 2701 tgtggctggg gcaccgatgg tcagcagccg aacgcttggc ctgcacttcc acccccggaa 2761 tggtctgcac caccaggtcc cggtcatgtt cgactatttc cacctgtctg tgatctcggt 2821 gaccgtccat gctgccctgg tggctctgca gcagccattg atcagtttta ctcgtccagg 2881 aagaggctcc tggcttggta aaggtggccc agacaccgga caagaacagt ctatcatttc 2941 tctggaaaac ttggtctttg gagctgggta ctgcaagccg acttcctcag agggaagctt 3001 ctacatcacc tctgagaact gcatgcagca cgcacacaag tggcaccgag acctgtgcct 3061 gttgctcctc cacgcttacc ggggtctccg tctccacttc ctggtgatca tgcgggacat 3121 cccagagctg ccacacacgg agctggaggc ccttgctgtt gaagaaacac tttctcagct 3181 gtgctcagag ctacagatgt tgaacaatcc agagaagatc gctgagcaga taagcaagga 3241 tctggcctgg ctcacgtccc acatgatgac tctgtggacc cagttcctgg acacagtcac 3301 tctgcactcc caagtgacca cttatctcac ccaggaacac cacaccctga gggtccgaag 3361 gttttctgag gccttctttt acatggagca ccaaaaactt gcagtcctga catttcagga 3421 gaatctgata cagacgcaca gccagctgtc cctggatatc cggaactcgg agtacctcac 3481 tagcatgccc ccgctgcctg cagagtgcct ggacatcgac ggcgattgga acaccctgcc 3541 ggtgatcttt gaggacagat acgtggactg ccctgcgaca gggcataact tgagtgttta 3601 tcctaatttt gatgttccag tgacaagtcc tacaataatg aatctgaaag acaaggaaga 3661 taactgtatg gtaaatagca atttatcttt tagggaagac cttgtcttgt ctaccataaa 3721 accatcccaa atggattctg atgaagaagt tataaggtgt ccagagccag gtgagaatgt 3781 ggccacacaa aatcatatgg acatgtgctc tgaatctcag gtgtatatat caattggtga 3841 atttcaaaac aaagcaggtg tgcctgaaga tgaatgttgg actggccaaa catctgatgc 3901 tgggacatat ccagtggcag atgtggatac ttctagaagg agtccaggtc cagaggatgg 3961 acaggcccca gtgctgacct acattgacgt aaaatctagc aataagaacc cctccagagc 4021 tgaacccctg gtggccttca atgctcagca tgagagtagg agctctagag ataagtatgg 4081 attagacagg actgggctaa gcaaagtggt agtaggtgga agccaccaaa atgccatctc 4141 ttcagacaaa acaactctcc atgaattaag tactctagga aagggaatag atcaagaggg 4201 gaagatggtg ctgctaagct tgaaactcac cccctctgag ccctgtgatc cactaagttc 4261 taccctgagg gagcccttag atattaggtc ttccctaaag gactctcaca cagaagagca 4321 ggaggaactc tcagtgctat ccggggtcat caagagatct tcatccatca tatctgattc 4381 aggcattgag agtgagccaa gctccgtcgc ctggtcagag gcccgaagca gggctctgga 4441 gttgcccagt gatcgggaag tcttgcaccc gtttgttcga agacatgccc tccaccggaa 4501 ctccctagag ggtggacaca cagaaagtaa cacaagtttg ccaagcggca tccaggcttc 4561 tctcacctcc attagctctt taccttttga ggaggatgag cgggaggtgg cactcactaa 4621 gttaaccaag tctgtatctg ctccccacat cagtagccca gaggaggctg ctgaagatgc 4681 ggacaccaag cagcaagatg gaggttttgc tgaaccttca gatatgcaca gcaagagcca 4741 aggttcccca ggatcttgct ctcaactttg tggtgactct ggaacagatg ctggagcaga 4801 ccatcccctg gtggagatag ttttagatgc tgacaaccag cagggccccg gatacataga 4861 catccccaaa gggaaaggga agcagtttga tgctcaagga cactgtcttc ctgatggcag 4921 gactgagaac actccaggtg ttggaaccaa aggtcttaat ttaaaaatac cacgcgtcat 4981 agcacttgaa aaccccagga ccagatctct tcatagagca cttgaggaaa ccccaaaggg 5041 catgcctaaa gacttgaatg tgggtcagca agctctttcc aacagtggca tctcagaggt 5101 tgagggtctc tctcaacatc aggtgcctga attgagctgt acgtcagctg ctgatgccat 5161 caacaggaac tcaacaggcc agcaaagcca aagcggttca ccttgcatta tggatgacac 5221 agcatttaat agaggagtga atgccttccc ggaggctaaa cataaagcag gcactgtgtg 5281 ccccactgtg acccattccg ttcattccca ggttttgaaa aaccaagagc tgaaggcagg 5341 cacttccatc atggggtccc atctgacttc tgcagagacc tttactctgg acagcctgaa 5401 ggctgtggag gttgtgaact tatctgtgtc ttgcactgcc acctgtctcc ctttctcatc 5461 tgtgcccaag gagacccctg ccagggctgg attctcttcc aaacagaccc tgtttcccat 5521 cacccatcag cctttgggat cctttggagt tgtttctacc cattccagca cgttggatga 5581 ggaagtcagt gagaggatgt ttagttttta tcaggccaaa gaaaaattta aaaaagaact 5641 gaagattgaa ggatttctgt acagtgactt aactgtacta gcttctgata taccatattt 5701 cccaccagag gaagaggaag aaaatttgga agatggaatt cacctggttg tctgtgtcca 5761 tggcctggat gggaacagtg cagacctccg gctggtaaag actttcatag aactggggct 5821 ccctggagga aaactggact tcctaatgtc tgaaaagaat cagatggaca catttgcaga 5881 ttttgatact atgacggatc ggttattgga tgaaatcatt caacacattc agttgtacaa 5941 cctctccata tcccgaatta gcttcattgg ccattctctt ggcaacatca tcatccgatc 6001 ggtcctcaca cggccccggt tccggtatta cctcaacaaa ctccacacgt tcctgtcact 6061 ctctgggcct cacctgggaa ccctgtacaa caacagcacc ctggttagta caggcttatg 6121 gctcatgcag aaactgaaga aatccgggtc tctactgcag ctgaccttca gggataatgc 6181 tgatttgcgc aaatgtttcc tctaccaact aagccaaaaa acagggctgc agtattttaa 6241 aaacgtcgtg ctggttgctt ctccccaaga ccgttatgtg ccatttcatt cagccaggat 6301 tgaaatgtgt aaaactgccc tcaaagacag acacacaggg ccagtttatg cagaaatgat 6361 caacaacctc ctgggccctc tggttgaagc caaggactgc actttaatcc gacacaacgt 6421 gttccacgcc ctgcccaaca ctgccaacac cctgatcggc cgagccgctc acatcgctgt 6481 gctggattca gaactcttcc tggagaagtt tttcttggtg gcaggactca actacttcaa 6541 gtagcggccg cgaaggatct gcgatcgctc cggtgcccgt cagtgggcag agcgcacatc 6601 gcccacagtc cccgagaagt tggggggagg ggtcggcaat tgaacgggtg cctagagaag 6661 gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg ctccgccttt ttcccgaggg 6721 tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac gttctttttc gcaacgggtt 6781 tgccgccaga acacagctga agcttcgagg ggctcgcatc tctccttcac gcgcccgccg 6841 ccctacctga ggccgccatc cacgccggtt gagtcgcgtt ctgccgcctc ccgcctgtgg 6901 tgcctcctga actgcgtccg ccgtctaggt aagtttaaag ctcaggtcga gaccgggcct 6961 ttgtccggcg ctcccttgga gcctacctag actcagccgg ctctccacgc tttgcctgac 7021 cctgcttgct caactctacg tctttgtttc gttttctgtt ctgcgccgtt acagatccaa 7081 gctgtgaccg gcgcctacgc tagacgccac catggagagc gacgagagcg gcctgcccgc 7141 catggagatc gagtgccgca tcaccggcac cctgaacggc gtggagttcg agctggtggg 7201 cggcggagag ggcaccccca agcagggccg catgaccaac aagatgaaga gcaccaaagg 7261 cgccctgacc ttcagcccct acctgctgag ccacgtgatg ggctacggct tctaccactt 7321 cggcacctac cccagcggct acgagaaccc cttcctgcac gccatcaaca acggcggcta 7381 caccaacacc cgcatcgaga agtacgagga cggcggcgtg ctgcacgtga gcttcagcta 7441 ccgctacgag gccggccgcg tgatcggcga cttcaaggtg gtgggcaccg gcttccccga 7501 ggacagcgtg atcttcaccg acaagatcat ccgcagcaac gccaccgtgg agcacctgca 7561 ccccatgggc gataacgtgc tggtgggcag cttcgcccgc accttcagcc tgcgcgacgg 7621 cggctactac agcttcgtgg tggacagcca catgcacttc aagagcgcca tccaccccag 7681 catcctgcag aacgggggcc ccatgttcgc cttccgccgc gtggaggagc tgcacagcaa 7741 caccgagctg ggcatcgtgg agtaccagca cgccttcaag acccccatcg ccttcgccag 7801 atcccgcgct cagtcgtcca attctgccgt ggacggcacc gccggacccg gctccaccgg 7861 atctcgcgag ggcagaggaa gtcttctaac atgcggtgac gtggaggaga atcccggccc 7921 tatgaccgag tacaagccca cggtgcgcct cgccacccgc gacgacgtcc ccagggccgt 7981 acgcaccctc gccgccgcgt tcgccgacta ccccgccacg cgccacaccg tcgatccgga 8041 ccgccacatc gagcgggtca ccgagctgca agaactcttc ctcacgcgcg tcgggctcga 8101 catcggcaag gtgtgggtcg cggacgacgg cgccgcggtg gcggtctgga ccacgccgga 8161 gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg cgcatggccg agttgagcgg 8221 ttcccggctg gccgcgcagc aacagatgga aggcctcctg gcgccgcacc ggcccaagga 8281 gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac caccagggca agggtctggg 8341 cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc gccggggtgc ccgccttcct 8401 ggagacctcc gcgccccgca acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc 8461 cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc ccggtgcctg 8521 aaatcaacct ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc 8581 tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg 8641 tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt 8701 gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac 8761 tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc 8821 tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct 8881 gttgggcact gacaattccg tggtgttgtc ggggaagctg acgtcctttc catggctgct 8941 cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct 9001 caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct 9061 ccgccttcgc cctcagacga gtcggatctc cctttggccg cctccccgcc tggtaccttt 9121 aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag aaaagggggg 9181 actggaaggg ctaattcact cccaacgaaa ataagatctg ctttttgctt gtactgggtc 9241 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct 9301 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga 9361 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtag 9421 tagttcatgt catcttatta ttcagtattt ataacttgca aagaaatgaa tatcagagag 9481 tgagaggaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa 9541 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa 9601 tgtatcttat catgtctggc tctagctatc ccgcccctaa ctccgcccag ttccgcccat 9661 tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc 9721 tctgagctat tccagaagta gtgaggaggc ttttttggag gcctagactt ttgcagagac 9781 ggcccaaatt cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca 9841 attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg 9901 agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg 9961 tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc 10021 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta 10081 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag 10141 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg 10201 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg 10261 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg 10321 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga 10381 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc 10441 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt 10501 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact 10561 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg 10621 cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt 10681 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt 10741 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct 10801 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg 10861 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt 10921 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt 10981 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc 11041 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg 11101 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc 11161 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg 11221 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca 11281 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga 11341 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct 11401 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg 11461 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca 11521 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata 11581 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct 11641 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact 11701 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa 11761 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc 11821 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga 11881 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga 11941 aaagtgccac ctgacgtcta agaaaccatt attatcatga cattaaccta taaaaatagg 12001 cgtatcacga ggccctttcg tctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac 12061 atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag cagacaagcc 12121 cgtcagggcg cgtcagcggg tgttggcggg tgtcggggct ggcttaacta tgcggcatca 12181 gagcagattg tactgagagt gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg 12241 agaaaatacc gcatcaggcg ccattcgcca ttcaggctgc gcaactgttg ggaagggcga 12301 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga 12361 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc 12421 aagctg //