LOCUS       V022270                12426 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V022270
VERSION     V022270
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     regulatory      2291..2300
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             2300..2323
                     /codon_start=1
                     /product="FLAG® epitope tag, followed by an enterokinase
                     cleavage site"
                     /transl_table=1
                     /translation="DYKDDDDK"
                     /label="FLAG"
     CDS             2324..6541
                     /label="FAM135B(NM_015912)"
                     /note="FAM135B(NM_015912)"
                     /gene="FAM135B"
     promoter        6585..6796
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             6809..7077
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      7106..7115
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             7136..7798
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             7868..7921
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             7922..8521
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    8522..9109
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             9183..9416
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    9488..9609
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      9628..9763
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(9801..9817)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    9825..9841
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(9849..9879)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    9894..9915
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(10203..10791)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(10962..11822)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(11823..11927)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     12401..12417
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc gccaccatgg attacaagga tgacgacgat aagatgtctg aaatacaagg
     2341 aacggttgag ttttcggtag agctacataa attttataat gtggatctct ttcagagagg
     2401 gtattaccag atccgagtga ccttgaaggt gtcttcaagg atcccccaca gactgagtgc
     2461 ctccatcgct gggcagacag agagcagcag cctgcattca gcctgtgtcc atgacagcac
     2521 cgtgcacagc cgggtctttc agatcttata ccggaatgaa gaggtaccca taaatgatgc
     2581 tgtggtcttc cgagttcatt tactcttggg tggtgaaagg atggaagacg cactgagtga
     2641 agtagatttt caactcaagg tggatctgca ctttacggac agtgaacagc agttgaggga
     2701 tgtggctggg gcaccgatgg tcagcagccg aacgcttggc ctgcacttcc acccccggaa
     2761 tggtctgcac caccaggtcc cggtcatgtt cgactatttc cacctgtctg tgatctcggt
     2821 gaccgtccat gctgccctgg tggctctgca gcagccattg atcagtttta ctcgtccagg
     2881 aagaggctcc tggcttggta aaggtggccc agacaccgga caagaacagt ctatcatttc
     2941 tctggaaaac ttggtctttg gagctgggta ctgcaagccg acttcctcag agggaagctt
     3001 ctacatcacc tctgagaact gcatgcagca cgcacacaag tggcaccgag acctgtgcct
     3061 gttgctcctc cacgcttacc ggggtctccg tctccacttc ctggtgatca tgcgggacat
     3121 cccagagctg ccacacacgg agctggaggc ccttgctgtt gaagaaacac tttctcagct
     3181 gtgctcagag ctacagatgt tgaacaatcc agagaagatc gctgagcaga taagcaagga
     3241 tctggcctgg ctcacgtccc acatgatgac tctgtggacc cagttcctgg acacagtcac
     3301 tctgcactcc caagtgacca cttatctcac ccaggaacac cacaccctga gggtccgaag
     3361 gttttctgag gccttctttt acatggagca ccaaaaactt gcagtcctga catttcagga
     3421 gaatctgata cagacgcaca gccagctgtc cctggatatc cggaactcgg agtacctcac
     3481 tagcatgccc ccgctgcctg cagagtgcct ggacatcgac ggcgattgga acaccctgcc
     3541 ggtgatcttt gaggacagat acgtggactg ccctgcgaca gggcataact tgagtgttta
     3601 tcctaatttt gatgttccag tgacaagtcc tacaataatg aatctgaaag acaaggaaga
     3661 taactgtatg gtaaatagca atttatcttt tagggaagac cttgtcttgt ctaccataaa
     3721 accatcccaa atggattctg atgaagaagt tataaggtgt ccagagccag gtgagaatgt
     3781 ggccacacaa aatcatatgg acatgtgctc tgaatctcag gtgtatatat caattggtga
     3841 atttcaaaac aaagcaggtg tgcctgaaga tgaatgttgg actggccaaa catctgatgc
     3901 tgggacatat ccagtggcag atgtggatac ttctagaagg agtccaggtc cagaggatgg
     3961 acaggcccca gtgctgacct acattgacgt aaaatctagc aataagaacc cctccagagc
     4021 tgaacccctg gtggccttca atgctcagca tgagagtagg agctctagag ataagtatgg
     4081 attagacagg actgggctaa gcaaagtggt agtaggtgga agccaccaaa atgccatctc
     4141 ttcagacaaa acaactctcc atgaattaag tactctagga aagggaatag atcaagaggg
     4201 gaagatggtg ctgctaagct tgaaactcac cccctctgag ccctgtgatc cactaagttc
     4261 taccctgagg gagcccttag atattaggtc ttccctaaag gactctcaca cagaagagca
     4321 ggaggaactc tcagtgctat ccggggtcat caagagatct tcatccatca tatctgattc
     4381 aggcattgag agtgagccaa gctccgtcgc ctggtcagag gcccgaagca gggctctgga
     4441 gttgcccagt gatcgggaag tcttgcaccc gtttgttcga agacatgccc tccaccggaa
     4501 ctccctagag ggtggacaca cagaaagtaa cacaagtttg ccaagcggca tccaggcttc
     4561 tctcacctcc attagctctt taccttttga ggaggatgag cgggaggtgg cactcactaa
     4621 gttaaccaag tctgtatctg ctccccacat cagtagccca gaggaggctg ctgaagatgc
     4681 ggacaccaag cagcaagatg gaggttttgc tgaaccttca gatatgcaca gcaagagcca
     4741 aggttcccca ggatcttgct ctcaactttg tggtgactct ggaacagatg ctggagcaga
     4801 ccatcccctg gtggagatag ttttagatgc tgacaaccag cagggccccg gatacataga
     4861 catccccaaa gggaaaggga agcagtttga tgctcaagga cactgtcttc ctgatggcag
     4921 gactgagaac actccaggtg ttggaaccaa aggtcttaat ttaaaaatac cacgcgtcat
     4981 agcacttgaa aaccccagga ccagatctct tcatagagca cttgaggaaa ccccaaaggg
     5041 catgcctaaa gacttgaatg tgggtcagca agctctttcc aacagtggca tctcagaggt
     5101 tgagggtctc tctcaacatc aggtgcctga attgagctgt acgtcagctg ctgatgccat
     5161 caacaggaac tcaacaggcc agcaaagcca aagcggttca ccttgcatta tggatgacac
     5221 agcatttaat agaggagtga atgccttccc ggaggctaaa cataaagcag gcactgtgtg
     5281 ccccactgtg acccattccg ttcattccca ggttttgaaa aaccaagagc tgaaggcagg
     5341 cacttccatc atggggtccc atctgacttc tgcagagacc tttactctgg acagcctgaa
     5401 ggctgtggag gttgtgaact tatctgtgtc ttgcactgcc acctgtctcc ctttctcatc
     5461 tgtgcccaag gagacccctg ccagggctgg attctcttcc aaacagaccc tgtttcccat
     5521 cacccatcag cctttgggat cctttggagt tgtttctacc cattccagca cgttggatga
     5581 ggaagtcagt gagaggatgt ttagttttta tcaggccaaa gaaaaattta aaaaagaact
     5641 gaagattgaa ggatttctgt acagtgactt aactgtacta gcttctgata taccatattt
     5701 cccaccagag gaagaggaag aaaatttgga agatggaatt cacctggttg tctgtgtcca
     5761 tggcctggat gggaacagtg cagacctccg gctggtaaag actttcatag aactggggct
     5821 ccctggagga aaactggact tcctaatgtc tgaaaagaat cagatggaca catttgcaga
     5881 ttttgatact atgacggatc ggttattgga tgaaatcatt caacacattc agttgtacaa
     5941 cctctccata tcccgaatta gcttcattgg ccattctctt ggcaacatca tcatccgatc
     6001 ggtcctcaca cggccccggt tccggtatta cctcaacaaa ctccacacgt tcctgtcact
     6061 ctctgggcct cacctgggaa ccctgtacaa caacagcacc ctggttagta caggcttatg
     6121 gctcatgcag aaactgaaga aatccgggtc tctactgcag ctgaccttca gggataatgc
     6181 tgatttgcgc aaatgtttcc tctaccaact aagccaaaaa acagggctgc agtattttaa
     6241 aaacgtcgtg ctggttgctt ctccccaaga ccgttatgtg ccatttcatt cagccaggat
     6301 tgaaatgtgt aaaactgccc tcaaagacag acacacaggg ccagtttatg cagaaatgat
     6361 caacaacctc ctgggccctc tggttgaagc caaggactgc actttaatcc gacacaacgt
     6421 gttccacgcc ctgcccaaca ctgccaacac cctgatcggc cgagccgctc acatcgctgt
     6481 gctggattca gaactcttcc tggagaagtt tttcttggtg gcaggactca actacttcaa
     6541 gtagcggccg cgaaggatct gcgatcgctc cggtgcccgt cagtgggcag agcgcacatc
     6601 gcccacagtc cccgagaagt tggggggagg ggtcggcaat tgaacgggtg cctagagaag
     6661 gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg ctccgccttt ttcccgaggg
     6721 tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac gttctttttc gcaacgggtt
     6781 tgccgccaga acacagctga agcttcgagg ggctcgcatc tctccttcac gcgcccgccg
     6841 ccctacctga ggccgccatc cacgccggtt gagtcgcgtt ctgccgcctc ccgcctgtgg
     6901 tgcctcctga actgcgtccg ccgtctaggt aagtttaaag ctcaggtcga gaccgggcct
     6961 ttgtccggcg ctcccttgga gcctacctag actcagccgg ctctccacgc tttgcctgac
     7021 cctgcttgct caactctacg tctttgtttc gttttctgtt ctgcgccgtt acagatccaa
     7081 gctgtgaccg gcgcctacgc tagacgccac catggagagc gacgagagcg gcctgcccgc
     7141 catggagatc gagtgccgca tcaccggcac cctgaacggc gtggagttcg agctggtggg
     7201 cggcggagag ggcaccccca agcagggccg catgaccaac aagatgaaga gcaccaaagg
     7261 cgccctgacc ttcagcccct acctgctgag ccacgtgatg ggctacggct tctaccactt
     7321 cggcacctac cccagcggct acgagaaccc cttcctgcac gccatcaaca acggcggcta
     7381 caccaacacc cgcatcgaga agtacgagga cggcggcgtg ctgcacgtga gcttcagcta
     7441 ccgctacgag gccggccgcg tgatcggcga cttcaaggtg gtgggcaccg gcttccccga
     7501 ggacagcgtg atcttcaccg acaagatcat ccgcagcaac gccaccgtgg agcacctgca
     7561 ccccatgggc gataacgtgc tggtgggcag cttcgcccgc accttcagcc tgcgcgacgg
     7621 cggctactac agcttcgtgg tggacagcca catgcacttc aagagcgcca tccaccccag
     7681 catcctgcag aacgggggcc ccatgttcgc cttccgccgc gtggaggagc tgcacagcaa
     7741 caccgagctg ggcatcgtgg agtaccagca cgccttcaag acccccatcg ccttcgccag
     7801 atcccgcgct cagtcgtcca attctgccgt ggacggcacc gccggacccg gctccaccgg
     7861 atctcgcgag ggcagaggaa gtcttctaac atgcggtgac gtggaggaga atcccggccc
     7921 tatgaccgag tacaagccca cggtgcgcct cgccacccgc gacgacgtcc ccagggccgt
     7981 acgcaccctc gccgccgcgt tcgccgacta ccccgccacg cgccacaccg tcgatccgga
     8041 ccgccacatc gagcgggtca ccgagctgca agaactcttc ctcacgcgcg tcgggctcga
     8101 catcggcaag gtgtgggtcg cggacgacgg cgccgcggtg gcggtctgga ccacgccgga
     8161 gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg cgcatggccg agttgagcgg
     8221 ttcccggctg gccgcgcagc aacagatgga aggcctcctg gcgccgcacc ggcccaagga
     8281 gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac caccagggca agggtctggg
     8341 cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc gccggggtgc ccgccttcct
     8401 ggagacctcc gcgccccgca acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc
     8461 cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc ccggtgcctg
     8521 aaatcaacct ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc
     8581 tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg
     8641 tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt
     8701 gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac
     8761 tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc
     8821 tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct
     8881 gttgggcact gacaattccg tggtgttgtc ggggaagctg acgtcctttc catggctgct
     8941 cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct
     9001 caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct
     9061 ccgccttcgc cctcagacga gtcggatctc cctttggccg cctccccgcc tggtaccttt
     9121 aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag aaaagggggg
     9181 actggaaggg ctaattcact cccaacgaaa ataagatctg ctttttgctt gtactgggtc
     9241 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct
     9301 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga
     9361 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtag
     9421 tagttcatgt catcttatta ttcagtattt ataacttgca aagaaatgaa tatcagagag
     9481 tgagaggaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
     9541 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
     9601 tgtatcttat catgtctggc tctagctatc ccgcccctaa ctccgcccag ttccgcccat
     9661 tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc
     9721 tctgagctat tccagaagta gtgaggaggc ttttttggag gcctagactt ttgcagagac
     9781 ggcccaaatt cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca
     9841 attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg
     9901 agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg
     9961 tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc
    10021 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
    10081 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
    10141 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
    10201 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
    10261 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
    10321 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
    10381 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
    10441 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
    10501 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
    10561 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
    10621 cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt
    10681 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
    10741 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
    10801 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
    10861 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
    10921 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
    10981 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
    11041 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
    11101 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
    11161 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
    11221 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca
    11281 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
    11341 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
    11401 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
    11461 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
    11521 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata
    11581 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
    11641 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
    11701 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
    11761 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
    11821 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
    11881 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
    11941 aaagtgccac ctgacgtcta agaaaccatt attatcatga cattaaccta taaaaatagg
    12001 cgtatcacga ggccctttcg tctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac
    12061 atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag cagacaagcc
    12121 cgtcagggcg cgtcagcggg tgttggcggg tgtcggggct ggcttaacta tgcggcatca
    12181 gagcagattg tactgagagt gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg
    12241 agaaaatacc gcatcaggcg ccattcgcca ttcaggctgc gcaactgttg ggaagggcga
    12301 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
    12361 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc
    12421 aagctg
//
