LOCUS       V034877                12703 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V034877
VERSION     V034877
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2317..6729
                     /label="FANCD2(NM_033084)"
                     /note="FANCD2(NM_033084)"
                     /gene="FANCD2"
     CDS             6736..6801
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        6845..7056
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             7069..7337
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      7366..7375
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             7396..8058
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             8128..8181
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             8182..8781
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    8782..9370
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             9444..9677
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    9749..9870
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      9910..10045
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(10078..10094)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    10102..10118
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(10126..10156)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    10171..10192
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(10480..11068)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(11239..12099)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(12100..12204)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     12678..12694
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc ctgatacgaa ctcggaattc gccaccatgg tttccaaaag aagactgtca
     2341 aaatctgagg ataaagagag cctgacagaa gatgcctcca aaaccaggaa gcaaccactt
     2401 tccaaaaaga caaagaaatc tcatattgct aatgaagttg aagaaaatga cagcatcttt
     2461 gtaaagcttc ttaagatatc aggaattatt cttaaaacgg gagagagtca gaatcaacta
     2521 gctgtggatc aaatagcttt ccaaaagaag ctctttcaga ccctgaggag acacccttcc
     2581 tatcccaaaa taatagaaga atttgttagt ggcctggagt cttacattga ggatgaagac
     2641 agtttcagga actgcctttt gtcttgtgag cgtctgcagg atgaggaagc cagtatgggt
     2701 gcatcttatt ctaagagtct catcaaactg cttctgggga ttgacatact gcagcctgcc
     2761 attatcaaaa ccttatttga gaagttgcca gaatattttt ttgaaaacaa gaacagtgat
     2821 gaaatcaaca tacctcgact cattgtcagt caactaaaat ggcttgacag agttgtggat
     2881 ggcaaggacc tcaccaccaa gatcatgcag ctgatcagta ttgctccaga gaacctgcag
     2941 catgacatca tcaccagcct acctgagatc ctaggggatt cccagcacgc tgatgtgggg
     3001 aaagaactca gtgacctact gatagagaat acttcactca ctgtcccaat cctggatgtc
     3061 ctttcaagcc tccgacttga cccaaacttc ctattgaagg ttcgccagtt ggtgatggat
     3121 aagttgtcgt ctattagatt ggaggattta cctgtgataa taaagttcat tcttcattcc
     3181 gtaacagcca tggatacact tgaggtaatt tctgagcttc gggagaagtt ggatctgcag
     3241 cattgtgttt tgccatcacg gttacaggct tcccaagtaa agttgaaaag taaaggacga
     3301 gcaagttcct caggaaatca agaaagcagc ggtcagagct gtattattct cctctttgat
     3361 gtaataaagt cagctattag atatgagaaa accatttcag aagcctggat taaggcaatt
     3421 gaaaacactg cctcagtatc tgaacacaag gtgtttgacc tggtgatgct tttcatcatc
     3481 tatagcacca atactcagac aaagaagtac attgacaggg tgctaagaaa taagattcga
     3541 tcaggctgca ttcaagaaca gctgctccag agtacattct ctgttcatta cttagttctt
     3601 aaggatatgt gttcatccat tctgtcgctg gctcagagtt tgcttcactc tctagaccag
     3661 agtataattt catttggcag tctcctatac aaatatgcat ttaagttttt tgacacgtac
     3721 tgccagcagg aagtggttgg tgccttagtg acccatatct gcagtgggaa tgaagctgaa
     3781 gttgatactg ccttagatgt ccttctagag ttggtagtgt taaacccatc tgctatgatg
     3841 atgaatgctg tctttgtaaa gggcatttta gattatctgg ataacatatc ccctcagcaa
     3901 atacgaaaac tcttctatgt tctcagcaca ctggcattta gcaaacagaa tgaagccagc
     3961 agccacatcc aggatgacat gcacttggtg ataagaaagc agctctctag caccgtattc
     4021 aagtacaagc tcattgggat tattggtgct gtgaccatgg ctggcatcat ggcggcagac
     4081 agaagtgaat cacctagttt gacccaagag agagccaacc tgagcgatga gcagtgcaca
     4141 caggtgacct ccttgttgca gttggttcat tcctgcagtg agcagtctcc tcaggcctct
     4201 gcactttact atgatgaatt tgccaacctg atccaacatg aaaagctgga tccaaaagcc
     4261 ctggaatggg ttgggcatac catctgtaat gatttccagg atgccttcgt agtggactcc
     4321 tgtgttgttc cggaaggtga ctttccattt cctgtgaaag cactgtacgg actggaagaa
     4381 tacgacactc aggatgggat tgccataaac ctcctgccgc tgctgttttc tcaggacttt
     4441 gcaaaagatg ggggtccggt gacctcacag gaatcaggcc aaaaattggt gtctccgctg
     4501 tgcctggctc cgtatttccg gttactgaga ctttgtgtgg agagacagca taacggaaac
     4561 ttggaggaga ttgatggtct actagattgt cctatattcc taactgacct ggagcctgga
     4621 gagaagttgg agtccatgtc tgctaaagag cgttcattca tgtgttctct catatttctt
     4681 actctcaact ggttccgaga gattgtaaat gccttctgcc aggaaacatc acctgagatg
     4741 aaggggaagg tgctcactcg gttaaagcac attgtagaat tgcaaataat cctggaaaag
     4801 tacttggcag tcaccccaga ctatgtccct cctcttggaa actttgatgt ggaaacttta
     4861 gatataacac ctcatactgt tactgctatt tcagcaaaaa tcagaaagaa aggaaaaata
     4921 gaaaggaaac aaaaaacaga tggcagcaag acatcctcct ctgacacact ttcagaagag
     4981 aaaaattcag aatgtgaccc tacgccatct catagaggcc agctaaacaa ggagttcaca
     5041 gggaaggaag aaaagacatc attgttacta cataattccc atgctttttt ccgagagctg
     5101 gacattgagg tcttctctat tctacattgt ggacttgtga cgaagttcat cttagatact
     5161 gaaatgcaca ctgaagctac agaagttgtg caacttgggc cccctgagct gcttttcttg
     5221 ctggaagatc tctcccagaa gctggagagt atgctgacac ctcctattgc caggagagtc
     5281 ccctttctca agaacaaagg aagccggaat attggattct cacatctcca acagagatct
     5341 gcccaagaaa ttgttcattg tgtttttcaa ctgctgaccc caatgtgtaa ccacctggag
     5401 aacattcaca actattttca gtgtttagct gctgagaatc acggtgtagt tgatggacca
     5461 ggagtgaaag ttcaggagta ccacataatg tcttcctgct atcagaggct gctgcagatt
     5521 tttcatgggc tttttgcttg gagtggattt tctcaacctg aaaatcagaa tttactgtat
     5581 tcagccctcc atgtccttag tagccgactg aaacagggag aacacagcca gcctttggag
     5641 gaactactca gccagagcgt ccattacttg cagaatttcc atcaaagcat tcccagtttc
     5701 cagtgtgctc tttatctcat cagacttttg atggttattt tggagaaatc aacagcttct
     5761 gctcagaaca aagaaaaaat tgcttccctt gccagacaat tcctctgtcg ggtgtggcca
     5821 agtggggata aagagaagag caacatctct aatgaccagc tccatgctct gctctgtatc
     5881 tacctggagc acacagagag cattctgaag gccatagagg agattgctgg tgttggtgtc
     5941 ccagaactga tcaactctcc taaagatgca tcttcctcca cattccctac actgaccagg
     6001 catacttttg ttgttttctt ccgtgtgatg atggctgaac tagagaagac ggtgaaaaaa
     6061 attgagcctg gcacagcagc agactcgcag cagattcatg aagagaaact cctctactgg
     6121 aacatggctg ttcgagactt cagtatcctc atcaacttga taaaggtatt tgatagtcat
     6181 cctgttctgc atgtatgttt gaagtatggg cgtctctttg tggaagcatt tctgaagcaa
     6241 tgtatgccgc tcctagactt cagttttaga aaacaccggg aagatgttct gagcttactg
     6301 gaaaccttcc agttggacac aaggctgctt catcacctgt gtgggcattc caagattcac
     6361 caggacacga gactcaccca acatgtgcct ctgctcaaaa agaccctgga acttttagtt
     6421 tgcagagtca aagctatgct cactctcaac aattgtagag aggctttctg gctgggcaat
     6481 ctaaaaaacc gggacttgca gggtgaagag attaagtccc aaaattccca ggagagcaca
     6541 gcagatgaga gtgaggatga catgtcatcc caggcctcca agagcaaagc cactgaggta
     6601 tctctacaaa acccaccaga gtctggcact gatggttgca ttttgttaat tgttctaagt
     6661 tggtggagca gaactttgcc tacttatgtt tattgtcaaa tgcttctatg cccatttcca
     6721 ttccctccag gatccgacta caaagaccat gacggtgatt ataaagatca tgacatcgac
     6781 tacaaggatg acgatgacaa gtagcggccg cgaaggatct gcgatcgctc cggtgcccgt
     6841 cagtgggcag agcgcacatc gcccacagtc cccgagaagt tggggggagg ggtcggcaat
     6901 tgaacgggtg cctagagaag gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg
     6961 ctccgccttt ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac
     7021 gttctttttc gcaacgggtt tgccgccaga acacagctga agcttcgagg ggctcgcatc
     7081 tctccttcac gcgcccgccg ccctacctga ggccgccatc cacgccggtt gagtcgcgtt
     7141 ctgccgcctc ccgcctgtgg tgcctcctga actgcgtccg ccgtctaggt aagtttaaag
     7201 ctcaggtcga gaccgggcct ttgtccggcg ctcccttgga gcctacctag actcagccgg
     7261 ctctccacgc tttgcctgac cctgcttgct caactctacg tctttgtttc gttttctgtt
     7321 ctgcgccgtt acagatccaa gctgtgaccg gcgcctacgc tagacgccac catggagagc
     7381 gacgagagcg gcctgcccgc catggagatc gagtgccgca tcaccggcac cctgaacggc
     7441 gtggagttcg agctggtggg cggcggagag ggcaccccca agcagggccg catgaccaac
     7501 aagatgaaga gcaccaaagg cgccctgacc ttcagcccct acctgctgag ccacgtgatg
     7561 ggctacggct tctaccactt cggcacctac cccagcggct acgagaaccc cttcctgcac
     7621 gccatcaaca acggcggcta caccaacacc cgcatcgaga agtacgagga cggcggcgtg
     7681 ctgcacgtga gcttcagcta ccgctacgag gccggccgcg tgatcggcga cttcaaggtg
     7741 gtgggcaccg gcttccccga ggacagcgtg atcttcaccg acaagatcat ccgcagcaac
     7801 gccaccgtgg agcacctgca ccccatgggc gataacgtgc tggtgggcag cttcgcccgc
     7861 accttcagcc tgcgcgacgg cggctactac agcttcgtgg tggacagcca catgcacttc
     7921 aagagcgcca tccaccccag catcctgcag aacgggggcc ccatgttcgc cttccgccgc
     7981 gtggaggagc tgcacagcaa caccgagctg ggcatcgtgg agtaccagca cgccttcaag
     8041 acccccatcg ccttcgccag atcccgcgct cagtcgtcca attctgccgt ggacggcacc
     8101 gccggacccg gctccaccgg atctcgcgag ggcagaggaa gtcttctaac atgcggtgac
     8161 gtggaggaga atcccggccc tatgaccgag tacaagccca cggtgcgcct cgccacccgc
     8221 gacgacgtcc ccagggccgt acgcaccctc gccgccgcgt tcgccgacta ccccgccacg
     8281 cgccacaccg tcgatccgga ccgccacatc gagcgggtca ccgagctgca agaactcttc
     8341 ctcacgcgcg tcgggctcga catcggcaag gtgtgggtcg cggacgacgg cgccgcggtg
     8401 gcggtctgga ccacgccgga gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg
     8461 cgcatggccg agttgagcgg ttcccggctg gccgcgcagc aacagatgga aggcctcctg
     8521 gcgccgcacc ggcccaagga gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac
     8581 caccagggca agggtctggg cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc
     8641 gccggggtgc ccgccttcct ggagacctcc gcgccccgca acctcccctt ctacgagcgg
     8701 ctcggcttca ccgtcaccgc cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg
     8761 acccgcaagc ccggtgcctg aaatcaacct ctggattaca aaatttgtga aagattgact
     8821 ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg
     8881 tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg
     8941 ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg
     9001 tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct cctttccggg
     9061 actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc
     9121 tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaaatca
     9181 tcgtcctttc cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc
     9241 tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct
     9301 ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc
     9361 gcctccccgc ctggtacctt taagaccaat gacttacaag gcagctgtag atcttagcca
     9421 ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaacgaa gataagatct
     9481 gctttttgct tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg
     9541 ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt
     9601 gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt
     9661 gtggaaaatc tctagcagta gtagttcatg tcatcttatt attcagtatt tataacttgc
     9721 aaagaaatga atatcagaga gtgagaggaa cttgtttatt gcagcttata atggttacaa
     9781 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg
     9841 tggtttgtcc aaactcatca atgtatctta tcatgtctgg ctctagctat cccgccccta
     9901 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga
     9961 ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag
    10021 tagtgaggag gcttttttgg aggcctagac ttttgcagag accaaattcg taatcatgtc
    10081 atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg
    10141 aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt
    10201 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg
    10261 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga
    10321 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
    10381 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
    10441 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
    10501 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
    10561 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
    10621 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
    10681 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
    10741 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
    10801 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
    10861 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
    10921 gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
    10981 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
    11041 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
    11101 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
    11161 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
    11221 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
    11281 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
    11341 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc
    11401 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
    11461 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
    11521 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc
    11581 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
    11641 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
    11701 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
    11761 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
    11821 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag
    11881 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
    11941 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
    12001 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
    12061 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
    12121 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
    12181 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga
    12241 aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc cctttcgtct
    12301 cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg agacggtcac
    12361 agcttgtctg taagcggatg ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt
    12421 tggcgggtgt cggggctggc ttaactatgc ggcatcagag cagattgtac tgagagtgca
    12481 ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga aaataccgca tcaggcgcca
    12541 ttcgccattc aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt
    12601 acgccagctg gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt
    12661 ttcccagtca cgacgttgta aaacgacggc cagtgccaag ctg
//
