LOCUS V026957 10570 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V026957 VERSION V026957 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers LTR 234..414 /note="truncated 5' long terminal repeat (LTR) from HIV-1" /label="5' LTR (truncated)" misc_feature 458..583 /note="packaging signal of human immunodeficiency virus type 1" /label="HIV-1 Ψ" misc_feature 1076..1309 /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." /label="RRE" CDS 1493..1537 /codon_start=1 /note="recognized by the 2H10 single-chain llama nanobody" /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /transl_table=1 /translation="KNEQELLELDKWASL" /label="gp41 peptide" misc_feature 1804..1921 /note="central polypurine tract and central termination sequence of HIV-1" /label="cPPT/CTS" promoter 2011..2214 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" CDS 2317..4596 /label="FAP(NM_004460)" /note="FAP(NM_004460)" /gene="FAP" CDS 4603..4668 /codon_start=1 /product="three tandem FLAG® epitope tags, followed by an enterokinase cleavage site" /transl_table=1 /translation="DYKDHDGDYKDHDIDYKDDDDK" /label="3xFLAG" promoter 4712..4923 /note="core promoter for human elongation factor EF-1α" /label="EF-1α core promoter" LTR 4936..5204 /note="truncated 5' long terminal repeat (LTR) from human T-cell leukemia virus (HTLV) type 1" /label="5' LTR (truncated)" regulatory 5233..5242 /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" /regulatory_class="other" /label="Kozak sequence" CDS 5263..5925 /codon_start=1 /product="green fluorescent protein 2 from Pontellina plumata, also known as ppluGFP2 (Shagin et al., 2004)" /transl_table=1 /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA" /label="CopGFP" CDS 5995..6048 /codon_start=1 /note="Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides." /product="2A peptide from Thosea asigna virus capsid protein" /protein_id="" /transl_table=1 /translation="EGRGSLLTCGDVEENPGP" /label="T2A" CDS 6049..6648 /codon_start=1 /gene="pac from Streptomyces alboniger" /note="confers resistance to puromycin" /product="puromycin N-acetyltransferase" /transl_table=1 /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*" /label="PuroR" misc_feature 6649..7237 /note="woodchuck hepatitis virus posttranscriptional regulatory element" /label="WPRE" LTR 7311..7544 /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" /label="3' LTR (ΔU3)" polyA_signal 7616..7737 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" rep_origin 7777..7912 /note="SV40 origin of replication" /label="SV40 ori" primer_bind complement(7945..7961) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 7969..7985 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(7993..8023) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 8038..8059 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(8347..8935) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(9106..9966) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(9967..10071) /gene="bla" /label="AmpR promoter" primer_bind 10545..10561 /note="common sequencing primer, one of multiple similar variants" /label="M13 fwd" ORIGIN 1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc ctgatacgaa ctcggaattc gccaccatga agacttgggt aaaaatcgta 2341 tttggagttg ccacctctgc tgtgcttgcc ttattggtga tgtgcattgt cttacgccct 2401 tcaagagttc ataactctga agaaaataca atgagagcac tcacactgaa ggatatttta 2461 aatggaacat tttcttataa aacatttttt ccaaactgga tttcaggaca agaatatctt 2521 catcaatctg cagataacaa tatagtactt tataatattg aaacaggaca atcatatacc 2581 attttgagta atagaaccat gaaaagtgtg aatgcttcaa attacggctt atcacctgat 2641 cggcaatttg tatatctaga aagtgattat tcaaagcttt ggagatactc ttacacagca 2701 acatattaca tctatgacct tagcaatgga gaatttgtaa gaggaaatga gcttcctcgt 2761 ccaattcagt atttatgctg gtcgcctgtt gggagtaaat tagcatatgt ctatcaaaac 2821 aatatctatt tgaaacaaag accaggagat ccaccttttc aaataacatt taatggaaga 2881 gaaaataaaa tatttaatgg aatcccagac tgggtttatg aagaggaaat gcttgctaca 2941 aaatatgctc tctggtggtc tcctaatgga aaatttttgg catatgcgga atttaatgat 3001 acggatatac cagttattgc ctattcctat tatggcgatg aacaatatcc tagaacaata 3061 aatattccat acccaaaggc tggagctaag aatcccgttg ttcggatatt tattatcgat 3121 accacttacc ctgcgtatgt aggtccccag gaagtgcctg ttccagcaat gatagcctca 3181 agtgattatt atttcagttg gctcacgtgg gttactgatg aacgagtatg tttgcagtgg 3241 ctaaaaagag tccagaatgt ttcggtcctg tctatatgtg acttcaggga agactggcag 3301 acatgggatt gtccaaagac ccaggagcat atagaagaaa gcagaactgg atgggctggt 3361 ggattctttg tttcaacacc agttttcagc tatgatgcca tttcgtacta caaaatattt 3421 agtgacaagg atggctacaa acatattcac tatatcaaag acactgtgga aaatgctatt 3481 caaattacaa gtggcaagtg ggaggccata aatatattca gagtaacaca ggattcactg 3541 ttttattcta gcaatgaatt tgaagaatac cctggaagaa gaaacatcta cagaattagc 3601 attggaagct atcctccaag caagaagtgt gttacttgcc atctaaggaa agaaaggtgc 3661 caatattaca cagcaagttt cagcgactac gccaagtact atgcacttgt ctgctacggc 3721 ccaggcatcc ccatttccac ccttcatgat ggacgcactg atcaagaaat taaaatcctg 3781 gaagaaaaca aggaattgga aaatgctttg aaaaatatcc agctgcctaa agaggaaatt 3841 aagaaacttg aagtagatga aattacttta tggtacaaga tgattcttcc tcctcaattt 3901 gacagatcaa agaagtatcc cttgctaatt caagtgtatg gtggtccctg cagtcagagt 3961 gtaaggtctg tatttgctgt taattggata tcttatcttg caagtaagga agggatggtc 4021 attgccttgg tggatggtcg aggaacagct ttccaaggtg acaaactcct ctatgcagtg 4081 tatcgaaagc tgggtgttta tgaagttgaa gaccagatta cagctgtcag aaaattcata 4141 gaaatgggtt tcattgatga aaaaagaata gccatatggg gctggtccta tggaggatac 4201 gtttcatcac tggcccttgc atctggaact ggtcttttca aatgtggtat agcagtggct 4261 ccagtctcca gctgggaata ttacgcgtct gtctacacag agagattcat gggtctccca 4321 acaaaggatg ataatcttga gcactataag aattcaactg tgatggcaag agcagaatat 4381 ttcagaaatg tagactatct tctcatccac ggaacagcag atgataatgt gcactttcaa 4441 aactcagcac agattgctaa agctctggtt aatgcacaag tggatttcca ggcaatgtgg 4501 tactctgacc agaaccacgg cttatccggc ctgtccacga accacttata cacccacatg 4561 acccacttcc taaagcagtg tttctctttg tcagacggat ccgactacaa agaccatgac 4621 ggtgattata aagatcatga catcgactac aaggatgacg atgacaagta gcggccgcga 4681 aggatctgcg atcgctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc 4741 gagaagttgg ggggaggggt cggcaattga acgggtgcct agagaaggtg gcgcggggta 4801 aactgggaaa gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg 4861 tatataagtg cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca 4921 cagctgaagc ttcgaggggc tcgcatctct ccttcacgcg cccgccgccc tacctgaggc 4981 cgccatccac gccggttgag tcgcgttctg ccgcctcccg cctgtggtgc ctcctgaact 5041 gcgtccgccg tctaggtaag tttaaagctc aggtcgagac cgggcctttg tccggcgctc 5101 ccttggagcc tacctagact cagccggctc tccacgcttt gcctgaccct gcttgctcaa 5161 ctctacgtct ttgtttcgtt ttctgttctg cgccgttaca gatccaagct gtgaccggcg 5221 cctacgctag acgccaccat ggagagcgac gagagcggcc tgcccgccat ggagatcgag 5281 tgccgcatca ccggcaccct gaacggcgtg gagttcgagc tggtgggcgg cggagagggc 5341 acccccaagc agggccgcat gaccaacaag atgaagagca ccaaaggcgc cctgaccttc 5401 agcccctacc tgctgagcca cgtgatgggc tacggcttct accacttcgg cacctacccc 5461 agcggctacg agaacccctt cctgcacgcc atcaacaacg gcggctacac caacacccgc 5521 atcgagaagt acgaggacgg cggcgtgctg cacgtgagct tcagctaccg ctacgaggcc 5581 ggccgcgtga tcggcgactt caaggtggtg ggcaccggct tccccgagga cagcgtgatc 5641 ttcaccgaca agatcatccg cagcaacgcc accgtggagc acctgcaccc catgggcgat 5701 aacgtgctgg tgggcagctt cgcccgcacc ttcagcctgc gcgacggcgg ctactacagc 5761 ttcgtggtgg acagccacat gcacttcaag agcgccatcc accccagcat cctgcagaac 5821 gggggcccca tgttcgcctt ccgccgcgtg gaggagctgc acagcaacac cgagctgggc 5881 atcgtggagt accagcacgc cttcaagacc cccatcgcct tcgccagatc ccgcgctcag 5941 tcgtccaatt ctgccgtgga cggcaccgcc ggacccggct ccaccggatc tcgcgagggc 6001 agaggaagtc ttctaacatg cggtgacgtg gaggagaatc ccggccctat gaccgagtac 6061 aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca gggccgtacg caccctcgcc 6121 gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg atccggaccg ccacatcgag 6181 cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg 6241 tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca cgccggagag cgtcgaagcg 6301 ggggcggtgt tcgccgagat cggcccgcgc atggccgagt tgagcggttc ccggctggcc 6361 gcgcagcaac agatggaagg cctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc 6421 ctggccaccg tcggcgtctc gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg 6481 ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga gacctccgcg 6541 ccccgcaacc tccccttcta cgagcggctc ggcttcaccg tcaccgccga cgtcgaggtg 6601 cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg gtgcctgaaa tcaacctctg 6661 gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta 6721 tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt 6781 ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc 6841 aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt 6901 gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg 6961 gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac 7021 aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc ctgtgttgcc 7081 acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac 7141 cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct 7201 cagacgagtc ggatctccct ttgggccgcc tccccgcctg gtacctttaa gaccaatgac 7261 ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac tggaagggct 7321 aattcactcc caacgaagat aagatctgct ttttgcttgt actgggtctc tctggttaga 7381 ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata 7441 aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta 7501 gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtagta gttcatgtca 7561 tcttattatt cagtatttat aacttgcaaa gaaatgaata tcagagagtg agaggaactt 7621 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa 7681 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca 7741 tgtctggctc tagctatccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt 7801 tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc 7861 gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg cctagacttt 7921 tgcagagacc aaattcgtaa tcatgtcata gctgtttcct gtgtgaaatt gttatccgct 7981 cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg 8041 agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct 8101 gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg 8161 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc 8221 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg 8281 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct 8341 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca 8401 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct 8461 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc 8521 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt 8581 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc 8641 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc 8701 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg 8761 gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc 8821 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag 8881 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga 8941 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat 9001 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag 9061 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat 9121 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc 9181 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat 9241 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag 9301 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg 9361 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc 9421 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca 9481 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg 9541 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc 9601 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta 9661 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc 9721 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg 9781 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc 9841 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc 9901 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat 9961 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag 10021 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc 10081 ccgaaaagtg ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa 10141 taggcgtatc acgaggccct ttcgtctcgc gcgtttcggt gatgacggtg aaaacctctg 10201 acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca 10261 agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggctggctta actatgcggc 10321 atcagagcag attgtactga gagtgcacca tatgcggtgt gaaataccgc acagatgcgt 10381 aaggagaaaa taccgcatca ggcgccattc gccattcagg ctgcgcaact gttgggaagg 10441 gcgatcggtg cgggcctctt cgctattacg ccagctggcg aaagggggat gtgctgcaag 10501 gcgattaagt tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa cgacggccag 10561 tgccaagctg //