LOCUS       V026957                10570 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V026957
VERSION     V026957
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2317..4596
                     /label="FAP(NM_004460)"
                     /note="FAP(NM_004460)"
                     /gene="FAP"
     CDS             4603..4668
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        4712..4923
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             4936..5204
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      5233..5242
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             5263..5925
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             5995..6048
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             6049..6648
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    6649..7237
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7311..7544
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    7616..7737
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      7777..7912
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(7945..7961)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    7969..7985
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(7993..8023)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8038..8059
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8347..8935)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9106..9966)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(9967..10071)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     10545..10561
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc ctgatacgaa ctcggaattc gccaccatga agacttgggt aaaaatcgta
     2341 tttggagttg ccacctctgc tgtgcttgcc ttattggtga tgtgcattgt cttacgccct
     2401 tcaagagttc ataactctga agaaaataca atgagagcac tcacactgaa ggatatttta
     2461 aatggaacat tttcttataa aacatttttt ccaaactgga tttcaggaca agaatatctt
     2521 catcaatctg cagataacaa tatagtactt tataatattg aaacaggaca atcatatacc
     2581 attttgagta atagaaccat gaaaagtgtg aatgcttcaa attacggctt atcacctgat
     2641 cggcaatttg tatatctaga aagtgattat tcaaagcttt ggagatactc ttacacagca
     2701 acatattaca tctatgacct tagcaatgga gaatttgtaa gaggaaatga gcttcctcgt
     2761 ccaattcagt atttatgctg gtcgcctgtt gggagtaaat tagcatatgt ctatcaaaac
     2821 aatatctatt tgaaacaaag accaggagat ccaccttttc aaataacatt taatggaaga
     2881 gaaaataaaa tatttaatgg aatcccagac tgggtttatg aagaggaaat gcttgctaca
     2941 aaatatgctc tctggtggtc tcctaatgga aaatttttgg catatgcgga atttaatgat
     3001 acggatatac cagttattgc ctattcctat tatggcgatg aacaatatcc tagaacaata
     3061 aatattccat acccaaaggc tggagctaag aatcccgttg ttcggatatt tattatcgat
     3121 accacttacc ctgcgtatgt aggtccccag gaagtgcctg ttccagcaat gatagcctca
     3181 agtgattatt atttcagttg gctcacgtgg gttactgatg aacgagtatg tttgcagtgg
     3241 ctaaaaagag tccagaatgt ttcggtcctg tctatatgtg acttcaggga agactggcag
     3301 acatgggatt gtccaaagac ccaggagcat atagaagaaa gcagaactgg atgggctggt
     3361 ggattctttg tttcaacacc agttttcagc tatgatgcca tttcgtacta caaaatattt
     3421 agtgacaagg atggctacaa acatattcac tatatcaaag acactgtgga aaatgctatt
     3481 caaattacaa gtggcaagtg ggaggccata aatatattca gagtaacaca ggattcactg
     3541 ttttattcta gcaatgaatt tgaagaatac cctggaagaa gaaacatcta cagaattagc
     3601 attggaagct atcctccaag caagaagtgt gttacttgcc atctaaggaa agaaaggtgc
     3661 caatattaca cagcaagttt cagcgactac gccaagtact atgcacttgt ctgctacggc
     3721 ccaggcatcc ccatttccac ccttcatgat ggacgcactg atcaagaaat taaaatcctg
     3781 gaagaaaaca aggaattgga aaatgctttg aaaaatatcc agctgcctaa agaggaaatt
     3841 aagaaacttg aagtagatga aattacttta tggtacaaga tgattcttcc tcctcaattt
     3901 gacagatcaa agaagtatcc cttgctaatt caagtgtatg gtggtccctg cagtcagagt
     3961 gtaaggtctg tatttgctgt taattggata tcttatcttg caagtaagga agggatggtc
     4021 attgccttgg tggatggtcg aggaacagct ttccaaggtg acaaactcct ctatgcagtg
     4081 tatcgaaagc tgggtgttta tgaagttgaa gaccagatta cagctgtcag aaaattcata
     4141 gaaatgggtt tcattgatga aaaaagaata gccatatggg gctggtccta tggaggatac
     4201 gtttcatcac tggcccttgc atctggaact ggtcttttca aatgtggtat agcagtggct
     4261 ccagtctcca gctgggaata ttacgcgtct gtctacacag agagattcat gggtctccca
     4321 acaaaggatg ataatcttga gcactataag aattcaactg tgatggcaag agcagaatat
     4381 ttcagaaatg tagactatct tctcatccac ggaacagcag atgataatgt gcactttcaa
     4441 aactcagcac agattgctaa agctctggtt aatgcacaag tggatttcca ggcaatgtgg
     4501 tactctgacc agaaccacgg cttatccggc ctgtccacga accacttata cacccacatg
     4561 acccacttcc taaagcagtg tttctctttg tcagacggat ccgactacaa agaccatgac
     4621 ggtgattata aagatcatga catcgactac aaggatgacg atgacaagta gcggccgcga
     4681 aggatctgcg atcgctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc
     4741 gagaagttgg ggggaggggt cggcaattga acgggtgcct agagaaggtg gcgcggggta
     4801 aactgggaaa gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg
     4861 tatataagtg cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca
     4921 cagctgaagc ttcgaggggc tcgcatctct ccttcacgcg cccgccgccc tacctgaggc
     4981 cgccatccac gccggttgag tcgcgttctg ccgcctcccg cctgtggtgc ctcctgaact
     5041 gcgtccgccg tctaggtaag tttaaagctc aggtcgagac cgggcctttg tccggcgctc
     5101 ccttggagcc tacctagact cagccggctc tccacgcttt gcctgaccct gcttgctcaa
     5161 ctctacgtct ttgtttcgtt ttctgttctg cgccgttaca gatccaagct gtgaccggcg
     5221 cctacgctag acgccaccat ggagagcgac gagagcggcc tgcccgccat ggagatcgag
     5281 tgccgcatca ccggcaccct gaacggcgtg gagttcgagc tggtgggcgg cggagagggc
     5341 acccccaagc agggccgcat gaccaacaag atgaagagca ccaaaggcgc cctgaccttc
     5401 agcccctacc tgctgagcca cgtgatgggc tacggcttct accacttcgg cacctacccc
     5461 agcggctacg agaacccctt cctgcacgcc atcaacaacg gcggctacac caacacccgc
     5521 atcgagaagt acgaggacgg cggcgtgctg cacgtgagct tcagctaccg ctacgaggcc
     5581 ggccgcgtga tcggcgactt caaggtggtg ggcaccggct tccccgagga cagcgtgatc
     5641 ttcaccgaca agatcatccg cagcaacgcc accgtggagc acctgcaccc catgggcgat
     5701 aacgtgctgg tgggcagctt cgcccgcacc ttcagcctgc gcgacggcgg ctactacagc
     5761 ttcgtggtgg acagccacat gcacttcaag agcgccatcc accccagcat cctgcagaac
     5821 gggggcccca tgttcgcctt ccgccgcgtg gaggagctgc acagcaacac cgagctgggc
     5881 atcgtggagt accagcacgc cttcaagacc cccatcgcct tcgccagatc ccgcgctcag
     5941 tcgtccaatt ctgccgtgga cggcaccgcc ggacccggct ccaccggatc tcgcgagggc
     6001 agaggaagtc ttctaacatg cggtgacgtg gaggagaatc ccggccctat gaccgagtac
     6061 aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca gggccgtacg caccctcgcc
     6121 gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg atccggaccg ccacatcgag
     6181 cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg
     6241 tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca cgccggagag cgtcgaagcg
     6301 ggggcggtgt tcgccgagat cggcccgcgc atggccgagt tgagcggttc ccggctggcc
     6361 gcgcagcaac agatggaagg cctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc
     6421 ctggccaccg tcggcgtctc gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg
     6481 ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga gacctccgcg
     6541 ccccgcaacc tccccttcta cgagcggctc ggcttcaccg tcaccgccga cgtcgaggtg
     6601 cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg gtgcctgaaa tcaacctctg
     6661 gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta
     6721 tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt
     6781 ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc
     6841 aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt
     6901 gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg
     6961 gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac
     7021 aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc ctgtgttgcc
     7081 acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac
     7141 cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct
     7201 cagacgagtc ggatctccct ttgggccgcc tccccgcctg gtacctttaa gaccaatgac
     7261 ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac tggaagggct
     7321 aattcactcc caacgaagat aagatctgct ttttgcttgt actgggtctc tctggttaga
     7381 ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata
     7441 aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta
     7501 gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtagta gttcatgtca
     7561 tcttattatt cagtatttat aacttgcaaa gaaatgaata tcagagagtg agaggaactt
     7621 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa
     7681 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca
     7741 tgtctggctc tagctatccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt
     7801 tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc
     7861 gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg cctagacttt
     7921 tgcagagacc aaattcgtaa tcatgtcata gctgtttcct gtgtgaaatt gttatccgct
     7981 cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg
     8041 agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct
     8101 gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg
     8161 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
     8221 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
     8281 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
     8341 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
     8401 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
     8461 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
     8521 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
     8581 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
     8641 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
     8701 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
     8761 gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc
     8821 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
     8881 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
     8941 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
     9001 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
     9061 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
     9121 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
     9181 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
     9241 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
     9301 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
     9361 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
     9421 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
     9481 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
     9541 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
     9601 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
     9661 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
     9721 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
     9781 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
     9841 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
     9901 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
     9961 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
    10021 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
    10081 ccgaaaagtg ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa
    10141 taggcgtatc acgaggccct ttcgtctcgc gcgtttcggt gatgacggtg aaaacctctg
    10201 acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca
    10261 agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggctggctta actatgcggc
    10321 atcagagcag attgtactga gagtgcacca tatgcggtgt gaaataccgc acagatgcgt
    10381 aaggagaaaa taccgcatca ggcgccattc gccattcagg ctgcgcaact gttgggaagg
    10441 gcgatcggtg cgggcctctt cgctattacg ccagctggcg aaagggggat gtgctgcaag
    10501 gcgattaagt tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa cgacggccag
    10561 tgccaagctg
//
