LOCUS       V028117                15823 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V028117
VERSION     V028117
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2317..9849
                     /label="FASN(NM_004104)"
                     /note="FASN(NM_004104)"
                     /gene="FASN"
     CDS             9856..9921
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        9965..10176
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             10189..10457
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      10486..10495
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             10516..11178
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             11248..11301
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             11302..11901
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    11902..12490
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             12564..12797
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    12869..12990
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      13030..13165
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(13198..13214)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    13222..13238
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(13246..13276)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    13291..13312
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(13600..14188)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(14359..15219)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(15220..15324)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     15798..15814
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc ctgatacgaa ctcggaattc gccaccatgg aggaggtggt gattgccggc
     2341 atgtccggga agctgccaga gtcggagaac ttgcaggagt tctgggacaa cctcatcggc
     2401 ggtgtggaca tggtcacgga cgatgaccgt cgctggaagg cggggctcta cggcctgccc
     2461 cggcggtccg gcaagctgaa ggacctgtct aggtttgatg cctccttctt cggagtccac
     2521 cccaagcagg cacacacgat ggaccctcag ctgcggctgc tgctggaagt cacctatgaa
     2581 gccatcgtgg acggaggcat caacccagat tcactccgag gaacacacac tggcgtctgg
     2641 gtgggcgtga gcggctctga gacctcggag gccctgagcc gagaccccga gacactcgtg
     2701 ggctacagca tggtgggctg ccagcgagcg atgatggcca accggctctc cttcttcttc
     2761 gacttcagag ggcccagcat cgcactggac acagcctgct cctccagcct gatggccctg
     2821 cagaacgcct accaggccat ccacagcggg cagtgccctg ccgccatcgt ggggggcatc
     2881 aatgtcctgc tgaagcccaa cacctccgtg cagttcttga ggctggggat gctcagcccc
     2941 gagggcacct gcaaggcctt cgacacagcg gggaatgggt actgccgctc ggagggtgtg
     3001 gtggccgtcc tgctgaccaa gaagtccctg gcccggcggg tgtacgccac catcctgaac
     3061 gccggcacca atacagatgg cttcaaggag caaggcgtga ccttcccctc aggggatatc
     3121 caggagcagc tcatccgctc gttgtaccag tcggccggag tggcccctga gtcatttgaa
     3181 tacatcgaag cccacggcac aggcaccaag gtgggcgacc cccaggagct gaatggcatc
     3241 acccgagccc tgtgcgccac ccgccaggag ccgctgctca tcggctccac caagtccaac
     3301 atggggcacc cggagccagc ctcggggctg gcagccctgg ccaaggtgct gctgtccctg
     3361 gagcacgggc tctgggcccc caacctgcac ttccatagcc ccaaccctga gatcccagcg
     3421 ctgttggatg ggcggctgca ggtggtggac cagcccctgc ccgtccgtgg cggcaacgtg
     3481 ggcatcaact cctttggctt cgggggctcc aacgtgcaca tcatcctgag gcccaacacg
     3541 cagccgcccc ccgcacccgc cccacatgcc accctgcccc gtctgctgcg ggccagcgga
     3601 cgcacccctg aggccgtgca gaagctgctg gagcagggcc tccggcacag ccaggacctg
     3661 gctttcctga gcatgctgaa cgacatcgcg gctgtccccg ccaccgccat gcccttccgt
     3721 ggctacgctg tgctgggtgg tgagcgcggt ggcccagagg tgcagcaggt gcccgctggc
     3781 gagcgcccgc tctggttcat ctgctctggg atgggcacac agtggcgcgg gatggggctg
     3841 agcctcatgc gcctggaccg cttccgagat tccatcctac gctccgatga ggctgtgaag
     3901 ccattcggcc tgaaggtgtc acagctgctg ctgagcacag acgagagcac ctttgatgac
     3961 atcgtccatt cgtttgtgag cctgactgcc atccagatag gcctcataga cctgctgagc
     4021 tgcatggggc tgaggccaga tggcatcgtc ggccactccc tgggggaggt ggcctgtggc
     4081 tacgccgacg gctgcctgtc ccaggaggag gccgtcctcg ctgcctactg gaggggacag
     4141 tgcatcaaag aagcccatct cccgccgggc gccatggcag ccgtgggctt gtcctgggag
     4201 gagtgtaaac agcgctgccc cccgggcgtg gtgcccgcct gccacaactc caaggacaca
     4261 gtcaccatct cgggacctca ggccccggtg tttgagttcg tggagcagct gaggaaggag
     4321 ggtgtgtttg ccaaggaggt gcggaccggc ggtatggcct tccactccta cttcatggag
     4381 gccatcgcac ccccactgct gcaggagctc aagaaggtga tccgggagcc gaagccacgt
     4441 tcagcccgct ggctcagcac ctctatcccc gaggcccagt ggcacagcag cctggcacgc
     4501 acgtcctccg ccgagtacaa tgtcaacaac ctggtgagcc ctgtgctgtt ccaggaggcc
     4561 ctgtggcacg tgcctgagca cgcggtggtg ctggagatcg cgccccacgc cctgctgcag
     4621 gctgtcctga agcgtggcct gaagccgagc tgcaccatca tccccctgat gaagaaggat
     4681 cacagggaca acctggagtt cttcctggcc ggcatcggca ggctgcacct ctcaggcatc
     4741 gacgccaacc ccaatgcctt gttcccacct gtggagttcc cagctccccg aggaactccc
     4801 ctcatctccc cactcatcaa gtgggaccac agcctggcct gggacgtgcc ggccgccgag
     4861 gacttcccca acggttcagg ttccccctca gccgccatct acaacatcga caccagctcc
     4921 gagtctcctg accactacct ggtggaccac accctcgacg gtcgcgtcct cttccccgcc
     4981 actggctacc tgagcatagt gtggaagacg ctggcccgcg ccctgggcct gggcgtcgag
     5041 cagctgcctg tggtgtttga ggatgtggtg ctgcaccagg ccaccatcct gcccaagact
     5101 gggacagtgt ccctggaggt acggctcctg gaggcctccc gtgccttcga ggtgtcagag
     5161 aacggcaacc tggtagtgag tgggaaggtg taccagtggg atgaccctga ccccaggctc
     5221 ttcgaccacc cggaaagccc cacccccaac cccacggagc ccctcttcct ggcccaggct
     5281 gaagtttaca aggagctgcg tctgcgtggc tacgactacg gccctcattt ccagggcatc
     5341 ctggaggcca gcctggaagg tgactcgggg aggctgctgt ggaaggataa ctgggtgagc
     5401 ttcatggaca ccatgctgca gatgtccatc ctgggctcgg ccaagcacgg cctgtacctg
     5461 cccacccgtg tcaccgccat ccacatcgac cctgccaccc acaggcagaa gctgtacaca
     5521 ctgcaggaca aggcccaagt ggctgacgtg gtggtgagca ggtggctgag ggtcacagtg
     5581 gccggaggcg tccacatctc cgggctccac actgagtcgg ccccgcggcg gcagcaggag
     5641 cagcaggtgc ccatcctgga gaagttttgc ttcactcccc acacggagga ggggtgcctg
     5701 tctgagcgcg ctgccctgca ggaggagctg caactgtgca aggggctggt gcaggcactg
     5761 cagaccaagg tgacccagca ggggctgaag atggtggtgc ccggactgga tggggcccag
     5821 atcccccggg acccctcaca gcaggaactg ccccggctgt tgtcggctgc ctgcaggctt
     5881 cagctcaacg ggaacctgca gctggagctg gcgcaggtgc tggcccagga gaggcccaag
     5941 ctgccagagg accctctgct cagcggcctc ctggactccc cggcactcaa ggcctgcctg
     6001 gacactgccg tggagaacat gcccagcctg aagatgaagg tggtggaggt gctggctggc
     6061 cacggtcacc tgtattcccg catcccaggc ctgctcagcc cccatcccct gctgcagctg
     6121 agctacacgg ccaccgaccg ccacccccag gccctggagg ctgcccaggc cgagctgcag
     6181 cagcacgacg ttgcccaggg ccagtgggat cccgcagacc ctgcccccag cgccctgggc
     6241 agcgccgacc tcctggtgtg caactgtgct gtggctgccc tcggggaccc ggcctcagct
     6301 ctcagcaaca tggtggctgc cctgagagaa gggggctttc tgctcctgca cacactgctc
     6361 cgggggcacc ccctcgggga catcgtggcc ttcctcacct ccactgagcc gcagtatggc
     6421 cagggcatcc tgagccagga cgcgtgggag agcctcttct ccagggtgtc gctgcgcctg
     6481 gtgggcctga agaagtcctt ctacggctcc acgctcttcc tgtgccgccg gcccaccccg
     6541 caggacagcc ccatcttcct gccggtggac gataccagct tccgctgggt ggagtctctg
     6601 aagggcatcc tggctgacga agactcttcc cggcctgtgt ggctgaaggc catcaactgt
     6661 gccacctcgg gcgtggtggg cttggtgaac tgtctccgcc gagagcccgg cgggaaccgc
     6721 ctccggtgtg tgctgctctc caacctcagc agcacctccc acgtcccgga ggtggacccg
     6781 ggctccgcag aactgcagaa ggtgttgcag ggagacctgg tgatgaacgt ctaccgcgac
     6841 ggggcctggg gggctttccg ccacttcctg ctggaggagg acaagcctga ggagccgacg
     6901 gcacatgcct ttgtgagcac cctcacccgg ggggacctgt cctccatccg ctgggtctgc
     6961 tcctcgctgc gccatgccca gcccacctgc cctggcgccc agctctgcac ggtctactac
     7021 gcctccctca acttccgcga catcatgctg gccactggca agctgtcccc tgatgccatc
     7081 ccagggaagt ggacctccca ggacagcctg ctaggtatgg agttctcggg ccgagacgcc
     7141 agcggcaagc gtgtgatggg actggtgcct gccaagggcc tggccacctc tgtcctgctg
     7201 tcaccggact tcctctggga tgtgccttcc aactggacgc tggaggaggc ggcctcggtg
     7261 cctgtcgtct acagcacggc ctactacgcg ctggtggtgc gtgggcgggt gcgccccggg
     7321 gagacgctgc tcatccactc gggctcgggc ggcgtgggcc aggccgccat cgccatcgcc
     7381 ctcagtctgg gctgccgcgt cttcaccacc gtggggtcgg ctgagaagcg ggcgtacctc
     7441 caggccaggt tcccccagct cgacagcacc agcttcgcca actcccggga cacatccttc
     7501 gagcagcatg tgctgtggca cacgggcggg aagggcgttg acctggtctt gaactccttg
     7561 gcggaagaga agctgcaggc cagcgtgagg tgcttggcta cgcacggtcg cttcctggaa
     7621 attggcaaat tcgacctttc tcagaaccac ccgctcggca tggctatctt cctgaagaac
     7681 gtgacattcc acggggtcct actggatgcg ttcttcaacg agagcagtgc tgactggcgg
     7741 gaggtgtggg cgcttgtgca ggccggcatc cgggatgggg tggtacggcc cctcaagtgc
     7801 acggtgttcc atggggccca ggtggaggac gccttccgct acatggccca agggaagcac
     7861 attggcaaag tcgtcgtgca ggtgcttgcg gaggagccgg aggcagtgct gaagggggcc
     7921 aaacccaagc tgatgtcggc catctccaag accttctgcc cggcccacaa gagctacatc
     7981 atcgctggtg gtctgggtgg cttcggcctg gagttggcgc agtggctgat acagcgtggg
     8041 gtgcagaagc tcgtgttgac ttctcgctcc gggatccgga caggctacca ggccaagcag
     8101 gtccgccggt ggaggcgcca gggcgtacag gtgcaggtgt ccaccagcaa catcagctca
     8161 ctggaggggg cccggggcct cattgccgag gcggcgcagc ttgggcccgt gggcggcgtc
     8221 ttcaacctgg ccgtggtctt gagagatggc ttgctggaga accagacccc agagttcttc
     8281 caggacgtct gcaagcccaa gtacagcggc accctgaacc tggacagggt gacccgagag
     8341 gcgtgccctg agctggacta ctttgtggtc ttctcctctg tgagctgcgg gcgtggcaat
     8401 gcgggacaga gcaactacgg ctttgccaat tccgccatgg agcgtatctg tgagaaacgc
     8461 cggcacgaag gcctcccagg cctggccgtg cagtggggcg ccatcggcga cgtgggcatt
     8521 ttggtggaga cgatgagcac caacgacacg atcgtcagtg gcacgctgcc ccagcgcatg
     8581 gcgtcctgcc tggaggtgct ggacctcttc ctgaaccagc cccacatggt cctgagcagc
     8641 tttgtgctgg ctgagaaggc tgcggcctat agggacaggg acagccagcg ggacctggtg
     8701 gaggccgtgg cacacatcct gggcatccgc gacttggctg ctgtcaacct ggacagctca
     8761 ctggcggacc tgggcctgga ctcgctcatg agcgtggagg tgcgccagac gctggagcgt
     8821 gagctcaacc tggtgctgtc cgtgcgcgag gtgcggcaac tcacgctccg gaaactgcag
     8881 gagctgtcct caaaggcgga tgaggccagc gagctggcat gccccacgcc caaggaggat
     8941 ggtctggccc agcagcagac tcagctgaac ctgcgctccc tgctggtgaa cccggagggc
     9001 cccaccctga tgcggctcaa ctccgtgcag agctcggagc ggcccctgtt cctggtgcac
     9061 ccaatcgagg gctccaccac cgtgttccac agcctggcct cccggctcag catccccacc
     9121 tatggcctgc agtgcacccg agctgcgccc cttgacagca tccacagcct ggctgcctac
     9181 tacatcgact gcatcaggca ggtgcagccc gagggcccct accgcgtggc cggctactcc
     9241 tacggggcct gcgtggcctt tgaaatgtgc tcccagctgc aggcccagca gagcccagcc
     9301 cccacccaca acagcctctt cctgttcgac ggctcgccca cctacgtact ggcctacacc
     9361 cagagctacc gggcaaagct gaccccaggc tgtgaggctg aggctgagac ggaggccata
     9421 tgcttcttcg tgcagcagtt cacggacatg gagcacaaca gggtgctgga ggcgctgctg
     9481 ccgctgaagg gcctagagga gcgtgtggca gccgccgtgg acctgatcat caagagccac
     9541 cagggcctgg accgccagga gctgagcttt gcggcccggt ccttctacta caagctgcgt
     9601 gccgctgagc agtacacacc caaggccaag taccatggca acgtgatgct actgcgcgcc
     9661 aagacgggtg gcgcctacgg cgaggacctg ggcgcggact acaacctctc ccaggtatgc
     9721 gacgggaaag tatccgtcca cgtcatcgag ggtgaccacc gcacgctgct ggagggcagc
     9781 ggcctggagt ccatcatcag catcatccac agctccctgg ctgagccacg cgtgagcgtg
     9841 cgggagggcg gatccgacta caaagaccat gacggtgatt ataaagatca tgacatcgac
     9901 tacaaggatg acgatgacaa gtagcggccg cgaaggatct gcgatcgctc cggtgcccgt
     9961 cagtgggcag agcgcacatc gcccacagtc cccgagaagt tggggggagg ggtcggcaat
    10021 tgaacgggtg cctagagaag gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg
    10081 ctccgccttt ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac
    10141 gttctttttc gcaacgggtt tgccgccaga acacagctga agcttcgagg ggctcgcatc
    10201 tctccttcac gcgcccgccg ccctacctga ggccgccatc cacgccggtt gagtcgcgtt
    10261 ctgccgcctc ccgcctgtgg tgcctcctga actgcgtccg ccgtctaggt aagtttaaag
    10321 ctcaggtcga gaccgggcct ttgtccggcg ctcccttgga gcctacctag actcagccgg
    10381 ctctccacgc tttgcctgac cctgcttgct caactctacg tctttgtttc gttttctgtt
    10441 ctgcgccgtt acagatccaa gctgtgaccg gcgcctacgc tagacgccac catggagagc
    10501 gacgagagcg gcctgcccgc catggagatc gagtgccgca tcaccggcac cctgaacggc
    10561 gtggagttcg agctggtggg cggcggagag ggcaccccca agcagggccg catgaccaac
    10621 aagatgaaga gcaccaaagg cgccctgacc ttcagcccct acctgctgag ccacgtgatg
    10681 ggctacggct tctaccactt cggcacctac cccagcggct acgagaaccc cttcctgcac
    10741 gccatcaaca acggcggcta caccaacacc cgcatcgaga agtacgagga cggcggcgtg
    10801 ctgcacgtga gcttcagcta ccgctacgag gccggccgcg tgatcggcga cttcaaggtg
    10861 gtgggcaccg gcttccccga ggacagcgtg atcttcaccg acaagatcat ccgcagcaac
    10921 gccaccgtgg agcacctgca ccccatgggc gataacgtgc tggtgggcag cttcgcccgc
    10981 accttcagcc tgcgcgacgg cggctactac agcttcgtgg tggacagcca catgcacttc
    11041 aagagcgcca tccaccccag catcctgcag aacgggggcc ccatgttcgc cttccgccgc
    11101 gtggaggagc tgcacagcaa caccgagctg ggcatcgtgg agtaccagca cgccttcaag
    11161 acccccatcg ccttcgccag atcccgcgct cagtcgtcca attctgccgt ggacggcacc
    11221 gccggacccg gctccaccgg atctcgcgag ggcagaggaa gtcttctaac atgcggtgac
    11281 gtggaggaga atcccggccc tatgaccgag tacaagccca cggtgcgcct cgccacccgc
    11341 gacgacgtcc ccagggccgt acgcaccctc gccgccgcgt tcgccgacta ccccgccacg
    11401 cgccacaccg tcgatccgga ccgccacatc gagcgggtca ccgagctgca agaactcttc
    11461 ctcacgcgcg tcgggctcga catcggcaag gtgtgggtcg cggacgacgg cgccgcggtg
    11521 gcggtctgga ccacgccgga gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg
    11581 cgcatggccg agttgagcgg ttcccggctg gccgcgcagc aacagatgga aggcctcctg
    11641 gcgccgcacc ggcccaagga gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac
    11701 caccagggca agggtctggg cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc
    11761 gccggggtgc ccgccttcct ggagacctcc gcgccccgca acctcccctt ctacgagcgg
    11821 ctcggcttca ccgtcaccgc cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg
    11881 acccgcaagc ccggtgcctg aaatcaacct ctggattaca aaatttgtga aagattgact
    11941 ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg
    12001 tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg
    12061 ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg
    12121 tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct cctttccggg
    12181 actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc
    12241 tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaaatca
    12301 tcgtcctttc cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc
    12361 tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct
    12421 ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc
    12481 gcctccccgc ctggtacctt taagaccaat gacttacaag gcagctgtag atcttagcca
    12541 ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaacgaa gataagatct
    12601 gctttttgct tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg
    12661 ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt
    12721 gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt
    12781 gtggaaaatc tctagcagta gtagttcatg tcatcttatt attcagtatt tataacttgc
    12841 aaagaaatga atatcagaga gtgagaggaa cttgtttatt gcagcttata atggttacaa
    12901 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg
    12961 tggtttgtcc aaactcatca atgtatctta tcatgtctgg ctctagctat cccgccccta
    13021 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga
    13081 ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag
    13141 tagtgaggag gcttttttgg aggcctagac ttttgcagag accaaattcg taatcatgtc
    13201 atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg
    13261 aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt
    13321 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg
    13381 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga
    13441 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
    13501 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
    13561 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
    13621 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
    13681 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
    13741 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
    13801 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
    13861 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
    13921 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
    13981 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
    14041 gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
    14101 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
    14161 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
    14221 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
    14281 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
    14341 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
    14401 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
    14461 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc
    14521 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
    14581 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
    14641 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc
    14701 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
    14761 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
    14821 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
    14881 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
    14941 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag
    15001 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
    15061 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
    15121 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
    15181 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
    15241 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
    15301 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga
    15361 aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc cctttcgtct
    15421 cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg agacggtcac
    15481 agcttgtctg taagcggatg ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt
    15541 tggcgggtgt cggggctggc ttaactatgc ggcatcagag cagattgtac tgagagtgca
    15601 ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga aaataccgca tcaggcgcca
    15661 ttcgccattc aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt
    15721 acgccagctg gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt
    15781 ttcccagtca cgacgttgta aaacgacggc cagtgccaag ctg
//
