LOCUS       V020183                 9775 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V020183
VERSION     V020183
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1798..1915
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2005..2208
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2289..4694
                     /label="FGFR4(NM_213647)"
                     /note="FGFR4(NM_213647)"
                     /gene="FGFR4"
     promoter        4745..4956
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             4969..5237
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     CDS             5264..5863
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    5870..6458
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             6532..6765
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    6837..6958
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      6977..7112
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(7150..7166)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    7174..7190
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(7198..7228)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    7243..7264
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(7552..8140)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(8311..9171)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(9172..9276)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     9750..9766
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggttaacttt
     1801 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc
     1861 aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa attttatcga
     1921 tactagtatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac
     1981 gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga
     2041 tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg
     2101 ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg
     2161 caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctcg tttagtgaac
     2221 cgtcagatcg cctggagacg ccatccacgc tgttttgacc tccatagaag attctagagc
     2281 tagccaccat gcggctgctg ctggccctgt tgggggtcct gctgagtgtg cctgggcctc
     2341 cagtcttgtc cctggaggcc tctgaggaag tggagcttga gccctgcctg gctcccagcc
     2401 tggagcagca agagcaggag ctgacagtag cccttgggca gcctgtgcgt ctgtgctgtg
     2461 ggcgggctga gcgtggtggc cactggtaca aggagggcag tcgcctggca cctgctggcc
     2521 gtgtacgggg ctggaggggc cgcctagaga ttgccagctt cctacctgag gatgctggcc
     2581 gctacctctg cctggcacga ggctccatga tcgtcctgca gaatctcacc ttgattacag
     2641 gtgactcctt gacctccagc aacgatgatg aggaccccaa gtcccatagg gacccctcga
     2701 ataggcacag ttacccccag caagcaccct actggacaca cccccagcgc atggagaaga
     2761 aactgcatgc agtacctgcg gggaacaccg tcaagttccg ctgtccagct gcaggcaacc
     2821 ccacgcccac catccgctgg cttaaggatg gacaggcctt tcatggggag aaccgcattg
     2881 gaggcattcg gctgcgccat cagcactgga gtctcgtgat ggagagcgtg gtgccctcgg
     2941 accgcggcac atacacctgc ctggtagaga acgctgtggg cagcatccgc tataactacc
     3001 tgctagatgt gctggagcgg tccccgcacc ggcccatcct gcaggccggg ctcccggcca
     3061 acaccacagc cgtggtgggc agcgacgtgg agctgctgtg caaggtgtac agcgatgccc
     3121 agccccacat ccagtggctg aagcacatcg tcatcaacgg cagcagcttc ggagccgacg
     3181 gtttccccta tgtgcaagtc ctaaagactg cagacatcaa tagctcagag gtggaggtcc
     3241 tgtacctgcg gaacgtgtca gccgaggacg caggcgagta cacctgcctc gcaggcaatt
     3301 ccatcggcct ctcctaccag tctgcctggc tcacggtgct gccagaggag gaccccacat
     3361 ggaccgcagc agcgcccgag gccaggtata cggacatcat cctgtacgcg tcgggctccc
     3421 tggccttggc tgtgctcctg ctgctggccg ggctgtatcg agggcaggcg ctccacggcc
     3481 ggcacccccg cccgcccgcc actgtgcaga agctctcccg cttccctctg gcccgacagt
     3541 tctccctgga gtcaggctct tccggcaagt caagctcatc cctggtacga ggcgtgcgtc
     3601 tctcctccag cggccccgcc ttgctcgccg gcctcgtgag tctagatcta cctctcgacc
     3661 cactatggga gttcccccgg gacaggctgg tgcttgggaa gcccctaggc gagggctgct
     3721 ttggccaggt agtacgtgca gaggcctttg gcatggaccc tgcccggcct gaccaagcca
     3781 gcactgtggc cgtcaagatg ctcaaagaca acgcctctga caaggacctg gccgacctgg
     3841 tctcggagat ggaggtgatg aagctgatcg gccgacacaa gaacatcatc aacctgcttg
     3901 gtgtctgcac ccaggaaggg cccctgtacg tgatcgtgga gtgcgccgcc aagggaaacc
     3961 tgcgggagtt cctgcgggcc cggcgccccc caggccccga cctcagcccc gacggtcctc
     4021 ggagcagtga ggggccgctc tccttcccag tcctggtctc ctgcgcctac caggtggccc
     4081 gaggcatgca gtatctggag tcccggaagt gtatccaccg ggacctggct gcccgcaatg
     4141 tgctggtgac tgaggacaat gtgatgaaga ttgctgactt tgggctggcc cgcggcgtcc
     4201 accacattga ctactataag aaaaccagca acggccgcct gcctgtgaag tggatggcgc
     4261 ccgaggcctt gtttgaccgg gtgtacacac accagagtga cgtgtggtct tttgggatcc
     4321 tgctatggga gatcttcacc ctcgggggct ccccgtatcc tggcatcccg gtggaggagc
     4381 tgttctcgct gctgcgggag ggacatcgga tggaccgacc cccacactgc cccccagagc
     4441 tgtacgggct gatgcgtgag tgctggcacg cagcgccctc ccagaggcct accttcaagc
     4501 agctggtgga ggcgctggac aaggtcctgc tggccgtctc tgaggagtac ctcgacctcc
     4561 gcctgacctt cggaccctat tccccctctg gtggggacgc cagcagcacc tgctcctcca
     4621 gcgattctgt cttcagccac gaccccctgc cattgggatc cagctccttc cccttcgggt
     4681 ctggggtgca gacatgagga tccgcggccg cgaaggatct gcgatcgctc cggtgcccgt
     4741 cagtgggcag agcgcacatc gcccacagtc cccgagaagt tggggggagg ggtcggcaat
     4801 tgaacgggtg cctagagaag gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg
     4861 ctccgccttt ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac
     4921 gttctttttc gcaacgggtt tgccgccaga acacagctga agcttcgagg ggctcgcatc
     4981 tctccttcac gcgcccgccg ccctacctga ggccgccatc cacgccggtt gagtcgcgtt
     5041 ctgccgcctc ccgcctgtgg tgcctcctga actgcgtccg ccgtctaggt aagtttaaag
     5101 ctcaggtcga gaccgggcct ttgtccggcg ctcccttgga gcctacctag actcagccgg
     5161 ctctccacgc tttgcctgac cctgcttgct caactctacg tctttgtttc gttttctgtt
     5221 ctgcgccgtt acagatccaa gctgtgaccg gcgcctacgc tagatgaccg agtacaagcc
     5281 cacggtgcgc ctcgccaccc gcgacgacgt cccccgggcc gtacgcaccc tcgccgccgc
     5341 gttcgccgac taccccgcca cgcgccacac cgtcgacccg gaccgccaca tcgagcgggt
     5401 caccgagctg caagaactct tcctcacgcg cgtcgggctc gacatcggca aggtgtgggt
     5461 cgcggacgac ggcgccgcgg tggcggtctg gaccacgccg gagagcgtcg aagcgggggc
     5521 ggtgttcgcc gagatcggcc cgcgcatggc cgagttgagc ggttcccggc tggccgcgca
     5581 gcaacagatg gaaggcctcc tggcgccgca ccggcccaag gagcccgcgt ggttcctggc
     5641 caccgtcggc gtctcgcccg accaccaggg caagggtctg ggcagcgccg tcgtgctccc
     5701 cggagtggag gcggccgagc gcgccggggt gcccgccttc ctggagacct ccgcgccccg
     5761 caacctcccc ttctacgagc ggctcggctt caccgtcacc gccgacgtcg aggtgcccga
     5821 aggaccgcgc acctggtgca tgacccgcaa gcccggtgcc tgagtcgaca atcaacctct
     5881 ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct
     5941 atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat
     6001 tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt
     6061 caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat
     6121 tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc
     6181 ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga
     6241 caattccgtg gtgttgtcgg ggaaatcatc gtcctttcct tggctgctcg cctgtgttgc
     6301 cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga
     6361 ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc gccttcgccc
     6421 tcagacgagt cggatctccc tttgggccgc ctccccgcct ggtaccttta agaccaatga
     6481 cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga ctggaagggc
     6541 taattcactc ccaacgaaaa taagatctgc tttttgcttg tactgggtct ctctggttag
     6601 accagatctg agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat
     6661 aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact
     6721 agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtagt agttcatgtc
     6781 atcttattat tcagtattta taacttgcaa agaaatgaat atcagagagt gagaggaact
     6841 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata
     6901 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc
     6961 atgtctggct ctagctatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca
     7021 tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt
     7081 ccagaagtag tgaggaggct tttttggagg cctagacttt tgcagagacg gcccaaattc
     7141 gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa
     7201 catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac
     7261 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca
     7321 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc
     7381 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
     7441 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
     7501 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
     7561 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
     7621 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
     7681 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
     7741 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
     7801 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
     7861 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
     7921 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
     7981 ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
     8041 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
     8101 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
     8161 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
     8221 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta
     8281 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat
     8341 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac
     8401 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg
     8461 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag
     8521 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt
     8581 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt
     8641 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt
     8701 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt
     8761 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct
     8821 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt
     8881 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac
     8941 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa
     9001 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa
     9061 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca
     9121 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct
     9181 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga
     9241 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc
     9301 tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag
     9361 gccctttcgt ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca tgcagctccc
     9421 ggagacggtc acagcttgtc tgtaagcgga tgccgggagc agacaagccc gtcagggcgc
     9481 gtcagcgggt gttggcgggt gtcggggctg gcttaactat gcggcatcag agcagattgt
     9541 actgagagtg caccatatgc ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg
     9601 catcaggcgc cattcgccat tcaggctgcg caactgttgg gaagggcgat cggtgcgggc
     9661 ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat taagttgggt
     9721 aacgccaggg ttttcccagt cacgacgttg taaaacgacg gccagtgcca agctg
//
