LOCUS       V029303                 9964 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V029303
VERSION     V029303
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2317..3990
                     /label="Fancc(NM_007985)"
                     /note="Fancc(NM_007985)"
                     /gene="Fancc"
     CDS             3997..4062
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        4106..4317
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             4330..4598
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      4627..4636
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             4657..5319
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             5389..5442
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             5443..6042
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    6043..6631
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             6705..6938
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    7010..7131
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      7171..7306
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(7339..7355)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    7363..7379
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(7387..7417)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    7432..7453
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(7741..8329)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(8500..9360)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(9361..9465)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     9939..9955
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc ctgatacgaa ctcggaattc gccaccatgg ctcaggagtc tgcagacctt
     2341 gcttctgact gtcagtcttg gctgcagaag ctttctgcat gggaacaggc ctcttctgag
     2401 gaaacccaga aggacacttg tcttcacttg tccgggttcc aggagttcct gaggcagatg
     2461 tatgaaatct tgaaggagat ggattctgat gcaatcctgg aaaggttccc cacaattggt
     2521 caactgttgg caaaagcttg ttggaatcct ctcatcttag catatgatga aagccaaaaa
     2581 attgtaatat ggtgcttatg ttgtctgatg aacaaagaac ctcggacttc tgcagagtca
     2641 ggacttaact cgtggatccg gggtttgtta tctcatgtac tttctgcatt cagattcgac
     2701 atgaaagaag tttgtctttt taccaaaagt cttggatatg agtctattga ttactatcct
     2761 agtttgctta aaaatatggt tttgtcatta gtgtctgagc tcagagagag tcatcttaat
     2821 ggactgagca ctcaaagtcg gatggctcct gagcgcatga tgtccctgtc agaagtttgt
     2881 gtccctcttg tcactctgcc tgatatggaa cccctggtag aggctctact cacctaccat
     2941 ggacatgagc cccaggaagt cctggctcct gagttcttcg aagctgtaaa tgaggccttc
     3001 ttgtcggaaa aaattgttct acccacgtcc tctgtggtca gcctctggtt tcggcatctc
     3061 cccagtcttg aaaaagcaac gctgcatctt tttgaaaagc ttttctccag caagataatt
     3121 tgcctgagaa ggatggagtg ctgtataaga gagtcattcc tgcctcaagc agcctgccaa
     3181 cctgccatct tcagaattgt tgatgaaatg ttcaggtttg tgctgctgga aactgacgga
     3241 gccccagaag tactagctgc tcttcaggtt ttcacatcgt gcttggtaga agctctgaaa
     3301 aaagaaaaca agcagctgac gtttgccctc aggacctact ttccttacgg tgctccatgt
     3361 cttgctgcag cgctgtccca gcaccctgaa gcaatcccac agggacaccg gctccagcct
     3421 ctgctgcaca tttcccaact cctcagagaa gcagttgaag actgtactcg tgggtctccg
     3481 cgaaatccct ttgagagctg gtttttgttt gttcactttg gaggatgggt tgacctggct
     3541 gtggcagagt tactgctgag ggaggaagct gagcctcctg ctggcctgct gtggctcttg
     3601 gtgttctatt acagcccaca ggatgggagt cagcagagag agcagagcat ggtggagctg
     3661 aaggtattaa tcaaccgtct cctgatgctg ctcagaagcg gccccctctc agctactgat
     3721 ctgcaggaag cagctgagag tcccagtgga gaccccagac cacctgtatg tggacagctg
     3781 gtcagacgcc ttcttcttag tctcttgctc tggaccccag aaggccatgc aattgtctgg
     3841 gaagctgtca cccatatggc ccacacggat gctgtaatcc atgagattat tggttttctt
     3901 gaccagacct tgtacagatc acaacatctt tgtgttgaag cctcgagaaa actggccaga
     3961 gacctcctaa aggagctgca agcccaggtc ggatccgact acaaagacca tgacggtgat
     4021 tataaagatc atgacatcga ctacaaggat gacgatgaca agtagcggcc gcgaaggatc
     4081 tgcgatcgct ccggtgcccg tcagtgggca gagcgcacat cgcccacagt ccccgagaag
     4141 ttggggggag gggtcggcaa ttgaacgggt gcctagagaa ggtggcgcgg ggtaaactgg
     4201 gaaagtgatg tcgtgtactg gctccgcctt tttcccgagg gtgggggaga accgtatata
     4261 agtgcagtag tcgccgtgaa cgttcttttt cgcaacgggt ttgccgccag aacacagctg
     4321 aagcttcgag gggctcgcat ctctccttca cgcgcccgcc gccctacctg aggccgccat
     4381 ccacgccggt tgagtcgcgt tctgccgcct cccgcctgtg gtgcctcctg aactgcgtcc
     4441 gccgtctagg taagtttaaa gctcaggtcg agaccgggcc tttgtccggc gctcccttgg
     4501 agcctaccta gactcagccg gctctccacg ctttgcctga ccctgcttgc tcaactctac
     4561 gtctttgttt cgttttctgt tctgcgccgt tacagatcca agctgtgacc ggcgcctacg
     4621 ctagacgcca ccatggagag cgacgagagc ggcctgcccg ccatggagat cgagtgccgc
     4681 atcaccggca ccctgaacgg cgtggagttc gagctggtgg gcggcggaga gggcaccccc
     4741 aagcagggcc gcatgaccaa caagatgaag agcaccaaag gcgccctgac cttcagcccc
     4801 tacctgctga gccacgtgat gggctacggc ttctaccact tcggcaccta ccccagcggc
     4861 tacgagaacc ccttcctgca cgccatcaac aacggcggct acaccaacac ccgcatcgag
     4921 aagtacgagg acggcggcgt gctgcacgtg agcttcagct accgctacga ggccggccgc
     4981 gtgatcggcg acttcaaggt ggtgggcacc ggcttccccg aggacagcgt gatcttcacc
     5041 gacaagatca tccgcagcaa cgccaccgtg gagcacctgc accccatggg cgataacgtg
     5101 ctggtgggca gcttcgcccg caccttcagc ctgcgcgacg gcggctacta cagcttcgtg
     5161 gtggacagcc acatgcactt caagagcgcc atccacccca gcatcctgca gaacgggggc
     5221 cccatgttcg ccttccgccg cgtggaggag ctgcacagca acaccgagct gggcatcgtg
     5281 gagtaccagc acgccttcaa gacccccatc gccttcgcca gatcccgcgc tcagtcgtcc
     5341 aattctgccg tggacggcac cgccggaccc ggctccaccg gatctcgcga gggcagagga
     5401 agtcttctaa catgcggtga cgtggaggag aatcccggcc ctatgaccga gtacaagccc
     5461 acggtgcgcc tcgccacccg cgacgacgtc cccagggccg tacgcaccct cgccgccgcg
     5521 ttcgccgact accccgccac gcgccacacc gtcgatccgg accgccacat cgagcgggtc
     5581 accgagctgc aagaactctt cctcacgcgc gtcgggctcg acatcggcaa ggtgtgggtc
     5641 gcggacgacg gcgccgcggt ggcggtctgg accacgccgg agagcgtcga agcgggggcg
     5701 gtgttcgccg agatcggccc gcgcatggcc gagttgagcg gttcccggct ggccgcgcag
     5761 caacagatgg aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg gttcctggcc
     5821 accgtcggcg tctcgcccga ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc
     5881 ggagtggagg cggccgagcg cgccggggtg cccgccttcc tggagacctc cgcgccccgc
     5941 aacctcccct tctacgagcg gctcggcttc accgtcaccg ccgacgtcga ggtgcccgaa
     6001 ggaccgcgca cctggtgcat gacccgcaag cccggtgcct gaaatcaacc tctggattac
     6061 aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga
     6121 tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc
     6181 tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa
     6241 cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc
     6301 acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc
     6361 atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc
     6421 gtggtgttgt cggggaaatc atcgtccttt ccttggctgc tcgcctgtgt tgccacctgg
     6481 attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct
     6541 tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg
     6601 agtcggatct ccctttgggc cgcctccccg cctggtacct ttaagaccaa tgacttacaa
     6661 ggcagctgta gatcttagcc actttttaaa agaaaagggg ggactggaag ggctaattca
     6721 ctcccaacga agataagatc tgctttttgc ttgtactggg tctctctggt tagaccagat
     6781 ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt
     6841 gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc
     6901 cctcagaccc ttttagtcag tgtggaaaat ctctagcagt agtagttcat gtcatcttat
     6961 tattcagtat ttataacttg caaagaaatg aatatcagag agtgagagga acttgtttat
     7021 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
     7081 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg
     7141 gctctagcta tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc
     7201 cattctccgc cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg
     7261 gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctaga cttttgcaga
     7321 gaccaaattc gtaatcatgt catagctgtt tcctgtgtga aattgttatc cgctcacaat
     7381 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag
     7441 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg
     7501 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc
     7561 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
     7621 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
     7681 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     7741 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     7801 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     7861 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     7921 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     7981 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     8041 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     8101 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     8161 taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac
     8221 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
     8281 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     8341 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
     8401 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
     8461 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
     8521 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
     8581 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
     8641 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
     8701 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
     8761 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
     8821 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
     8881 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
     8941 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
     9001 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
     9061 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
     9121 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
     9181 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
     9241 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
     9301 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
     9361 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
     9421 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
     9481 agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg
     9541 tatcacgagg ccctttcgtc tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat
     9601 gcagctcccg gagacggtca cagcttgtct gtaagcggat gccgggagca gacaagcccg
     9661 tcagggcgcg tcagcgggtg ttggcgggtg tcggggctgg cttaactatg cggcatcaga
     9721 gcagattgta ctgagagtgc accatatgcg gtgtgaaata ccgcacagat gcgtaaggag
     9781 aaaataccgc atcaggcgcc attcgccatt caggctgcgc aactgttggg aagggcgatc
     9841 ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt
     9901 aagttgggta acgccagggt tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa
     9961 gctg
//
