LOCUS       V029894                11560 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V029894
VERSION     V029894
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2317..5586
                     /label="Fcsk(NM_172283)"
                     /note="Fcsk(NM_172283)"
                     /gene="Fcsk"
     CDS             5593..5658
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        5702..5913
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             5926..6194
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      6223..6232
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             6253..6915
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             6985..7038
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             7039..7638
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    7639..8227
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             8301..8534
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    8606..8727
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      8767..8902
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(8935..8951)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8959..8975
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8983..9013)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    9028..9049
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(9337..9925)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(10096..10956)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10957..11061)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     11535..11551
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc ctgatacgaa ctcggaattc gccaccatgg agcagtcaga gggagtcaat
     2341 tggactgtca ttatcctgac atgccagtac aaggacagtg tccaggtctt tcagagagag
     2401 ctggaggtaa ggcagagacg ggagcagatt cctgcgggga cgatgttact ggctgtggag
     2461 gatccccaga ctcgagtcgg cagcggagga gccaccctca acgcactgct ggtggctgct
     2521 gaacacttga gtgcccgagc tggcttcact gtggtcacgt ccgatgtcct gcactctgcc
     2581 tggatcctca tcttgcacat gggccgagac ttccccttcg atgactgtgg cagggccttc
     2641 acttgcctcc ctgtggagaa cccacaggcc cctgtggagg ccttggtatg caacctggac
     2701 tgcctgttgg atatcatgac ccaccggctg ggtccaggtt ccccaccagg tgtgtgggtc
     2761 tgcagcaccg acatgcttct gtctgttcct ccaaaccctg ggatcagttg ggatggcttc
     2821 cggggagcca gagtgatcgc ctttcctggg agcctggcct atgcgttgaa ccacggtgtc
     2881 tacctcactg actcacaggg cttggttttg gacatttact accagggcac taaggcggag
     2941 atacaacgtt gtgtcggacc tgatgggctg gtaccattgg tctccggggt cgtcttcttc
     3001 tctgtggaga ctgctgagca cctcctagcc acccatgtga gcccaccgct ggatgcctgc
     3061 acctatatgg gcttggactc tggagcccag cctgtgcagc tgtctctgtt tttcgacatc
     3121 ctgctctgca tggctcggaa tatgagcagg gagaacttcc tggctgggcg gcccccggag
     3181 ttggggcaag gtgacatgga tgtagcaagt tacctgaagg gagcccgggc ccagctgtgg
     3241 agggagcttc gagatcagcc cctcacaatg gtgtatgtcc ctgacggcgg ctacagctac
     3301 atgacgactg atgccaccga gttcctgcac agactcacga tgcctggagt agctgtggca
     3361 cagattgttc actcccaggt ggaggagcca cagctgctag aggctacgtg ctcggtggtc
     3421 agctgcctgc tcgagggccc tgtgcacctg gggcctcgaa gtgtcctgca gcactgtcac
     3481 ctgaggggcc ccattcgcat cggcgctggc tgctttgtga gtggtctgga tacagcccac
     3541 tcggaggcac tgcatggcct ggagctccat gatgtcatcc tgcagggaca ccatgtgcgg
     3601 ctgcatggct ccctgagccg tgtatttact cttgctggcc gtctggacag ctgggaaaga
     3661 cagggggcag gcatgtatct caacatgtcc tggaatgagt tcttcaagaa gacaggcatt
     3721 cgagactggg acctgtggga cccagataca cccccctcag atcgatgcct cctcactgcc
     3781 cgccttttcc ctgtgctcca ccccacgagg gccctggggc cccaggatgt gctgtggatg
     3841 ctgcaccccc gcaaacacag aggtgaggcc cttcgggcct ggcgagcctc ctggcgtctg
     3901 tcctgggagc agctgcaacc ttgtgtggac cgggctgcca cactggactt ccgccgagat
     3961 ctgttcttct gccaggcctt gcagaaggca aggcatgtgt tagaggcgcg gcaggacctc
     4021 tgcctacgtc cactgatccg ggccgctgtc ggggaaggtt gctctgggcc cctgctggcc
     4081 acacttgaca aggttgcagc tggggcagaa gatcctggcg tggcagcccg ggctctggct
     4141 tgtgtggccg atgtgctggg ctgcatggca gagggccgag gaggcttgcg cagtgggcca
     4201 gctgccaacc ctgagtggat tcagcctttc tcatacttgg agtgtggaga cctgatgagg
     4261 ggtgtggagg cgcttgccca ggagagagag aagtggctga ccaggcctgc cttgctggtt
     4321 cgagctgccc gccattacga gggggccgag cagatcctga tccgccaggc tgtgatgaca
     4381 gcccggcact tcgtctccac ccagcccgtg gagctgcccg cacccgggca gtgggtggtg
     4441 actgagtgcc cagcccgtgt ggatttctct gggggctgga gtgacacacc gcccattgcc
     4501 tatgagcttg gtggagcagt gttgggcctg gctgtgcggg tggatggccg ccggcccatc
     4561 ggggccaaag cacgccgcat cccggagcct gagctctggc tggcagtggg acctcggcag
     4621 gatgagatga ccatgaggat agtgtgccgg agcctggatg acctgcggga ttactgccag
     4681 cctcatgccc caggggcctt gctgaaggca gcctttatct gtgctggcat tgtgcatctc
     4741 cactcagagc tccctctgct tgaacagttg ttacactcct ttaatggtgg ctttgagctg
     4801 cacacgtggt cagagctgcc gcacggctct ggtcttggca ccagcagcat cctggcaggg
     4861 gctgccctgg ctgccttaca gcgggctgca ggccgggcag tgggcacgga ggctctcatc
     4921 cacgcagtgc tgcacctgga gcaggtgctc accacaggag gtggctggca ggaccaagtc
     4981 agtggcctaa tgcctggcat caaagtgggg cgctcccggg cccagctgcc cctcaaggtg
     5041 gaggtggagg aaatcactgt gcctgagggc tttgtccaga agatcaatga ccatctgctc
     5101 ctggtttata ccggcaagac ccgattggcc cggaatctgc tgcaggacgt gctgaggaac
     5161 tggtacgctc ggttgcccgt tgtggtacag aatgcccgca gactggtgcg acagaccgag
     5221 aagtgcgctg aagctttccg ccaaggaaac ctgcctctgc tgggacagta cctgacctca
     5281 tactgggagc agaagaagct tatggcccca ggctgcgagc cgctggccgt gcagcgaatg
     5341 atggatgtcc tggccccgta tgcgtatggc caaagcctgg caggggcagg tggtgggggc
     5401 tttctctatc tattgaccaa ggaaccccgg cagaaagaga ctctggaagc tgtcctggcc
     5461 aaggctgagg gccttggcaa ctacagtgtc cacctggtgg aagtggatcc tcagggcctg
     5521 agcctgcagc tgctgggaca cgacacccgt ctttgtgggg ccgggccctc tgaagtgggc
     5581 accaccggat ccgactacaa agaccatgac ggtgattata aagatcatga catcgactac
     5641 aaggatgacg atgacaagta gcggccgcga aggatctgcg atcgctccgg tgcccgtcag
     5701 tgggcagagc gcacatcgcc cacagtcccc gagaagttgg ggggaggggt cggcaattga
     5761 acgggtgcct agagaaggtg gcgcggggta aactgggaaa gtgatgtcgt gtactggctc
     5821 cgcctttttc ccgagggtgg gggagaaccg tatataagtg cagtagtcgc cgtgaacgtt
     5881 ctttttcgca acgggtttgc cgccagaaca cagctgaagc ttcgaggggc tcgcatctct
     5941 ccttcacgcg cccgccgccc tacctgaggc cgccatccac gccggttgag tcgcgttctg
     6001 ccgcctcccg cctgtggtgc ctcctgaact gcgtccgccg tctaggtaag tttaaagctc
     6061 aggtcgagac cgggcctttg tccggcgctc ccttggagcc tacctagact cagccggctc
     6121 tccacgcttt gcctgaccct gcttgctcaa ctctacgtct ttgtttcgtt ttctgttctg
     6181 cgccgttaca gatccaagct gtgaccggcg cctacgctag acgccaccat ggagagcgac
     6241 gagagcggcc tgcccgccat ggagatcgag tgccgcatca ccggcaccct gaacggcgtg
     6301 gagttcgagc tggtgggcgg cggagagggc acccccaagc agggccgcat gaccaacaag
     6361 atgaagagca ccaaaggcgc cctgaccttc agcccctacc tgctgagcca cgtgatgggc
     6421 tacggcttct accacttcgg cacctacccc agcggctacg agaacccctt cctgcacgcc
     6481 atcaacaacg gcggctacac caacacccgc atcgagaagt acgaggacgg cggcgtgctg
     6541 cacgtgagct tcagctaccg ctacgaggcc ggccgcgtga tcggcgactt caaggtggtg
     6601 ggcaccggct tccccgagga cagcgtgatc ttcaccgaca agatcatccg cagcaacgcc
     6661 accgtggagc acctgcaccc catgggcgat aacgtgctgg tgggcagctt cgcccgcacc
     6721 ttcagcctgc gcgacggcgg ctactacagc ttcgtggtgg acagccacat gcacttcaag
     6781 agcgccatcc accccagcat cctgcagaac gggggcccca tgttcgcctt ccgccgcgtg
     6841 gaggagctgc acagcaacac cgagctgggc atcgtggagt accagcacgc cttcaagacc
     6901 cccatcgcct tcgccagatc ccgcgctcag tcgtccaatt ctgccgtgga cggcaccgcc
     6961 ggacccggct ccaccggatc tcgcgagggc agaggaagtc ttctaacatg cggtgacgtg
     7021 gaggagaatc ccggccctat gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac
     7081 gacgtcccca gggccgtacg caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc
     7141 cacaccgtcg atccggaccg ccacatcgag cgggtcaccg agctgcaaga actcttcctc
     7201 acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg
     7261 gtctggacca cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc
     7321 atggccgagt tgagcggttc ccggctggcc gcgcagcaac agatggaagg cctcctggcg
     7381 ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac
     7441 cagggcaagg gtctgggcag cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc
     7501 ggggtgcccg ccttcctgga gacctccgcg ccccgcaacc tccccttcta cgagcggctc
     7561 ggcttcaccg tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc
     7621 cgcaagcccg gtgcctgaaa tcaacctctg gattacaaaa tttgtgaaag attgactggt
     7681 attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat
     7741 catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg
     7801 tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt
     7861 gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact
     7921 ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc
     7981 tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaaatcatcg
     8041 tcctttcctt ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc
     8101 tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg
     8161 cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc
     8221 tccccgcctg gtacctttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt
     8281 tttaaaagaa aaggggggac tggaagggct aattcactcc caacgaagat aagatctgct
     8341 ttttgcttgt actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta
     8401 actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg
     8461 tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg
     8521 gaaaatctct agcagtagta gttcatgtca tcttattatt cagtatttat aacttgcaaa
     8581 gaaatgaata tcagagagtg agaggaactt gtttattgca gcttataatg gttacaaata
     8641 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
     8701 tttgtccaaa ctcatcaatg tatcttatca tgtctggctc tagctatccc gcccctaact
     8761 ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta
     8821 atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag
     8881 tgaggaggct tttttggagg cctagacttt tgcagagacc aaattcgtaa tcatgtcata
     8941 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag
     9001 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg
     9061 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca
     9121 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc
     9181 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
     9241 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
     9301 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
     9361 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
     9421 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
     9481 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
     9541 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
     9601 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
     9661 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
     9721 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac
     9781 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
     9841 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
     9901 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
     9961 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
    10021 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
    10081 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
    10141 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
    10201 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
    10261 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
    10321 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
    10381 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt
    10441 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
    10501 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
    10561 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
    10621 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
    10681 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
    10741 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
    10801 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
    10861 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
    10921 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
    10981 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
    11041 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac
    11101 cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtctcgc
    11161 gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga cggtcacagc
    11221 ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg
    11281 cgggtgtcgg ggctggctta actatgcggc atcagagcag attgtactga gagtgcacca
    11341 tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcgccattc
    11401 gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg
    11461 ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc
    11521 ccagtcacga cgttgtaaaa cgacggccag tgccaagctg
//
